Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,224,239 A→T 5.0% intergenic (+332/+40) ycgI → / ← minE pseudogene/cell division topological specificity factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,224,2390AT 5.0% 359.0 / 6.6 120intergenic (+332/+40)ycgI/minEpseudogene/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base A (64/50);  new base T (2/4);  total (66/54)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.85e-01

Only 100 of 123 total aligned reads displayed.

ACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTAC  >  NC_000913/1224090‑1224386
                                                                                                                                                     |                                                                                                                                                   
aCTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAgaga                                                                                                                                                     >  2:470928/1‑150 (MQ=255)
 cTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGAt                                                                                                                                                    >  2:1012583/1‑150 (MQ=255)
 cTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACAACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGAt                                                                                                                                                    <  1:284100/150‑1 (MQ=255)
   cAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTTAAGGGGGAGGTGGAAATAGCAATGAGGAGTCTCAGCAAGAATACTCGCCGCATTACCACAACGTGGATGAGAGGGATGAAAAACTCTAGGAAGAGATaa                                                                                                                                                  >  2:338259/1‑150 (MQ=255)
   cAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATaa                                                                                                                                                  >  2:1448346/1‑150 (MQ=255)
   cAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATaa                                                                                                                                                  >  2:600550/1‑150 (MQ=255)
         gtcATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTACGGGGTAAAGGGGGAGGGGGAAATAGCAATGCGGAGAATCAGCAAGAATACTCGCCGCTTTACCACAACGGGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGc                                                                                                                                            <  1:1678565/148‑1 (MQ=255)
           cATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCt                                                                                                                                          >  1:1166924/1‑150 (MQ=255)
             tAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTg                                                                                                                                        <  1:612850/150‑1 (MQ=255)
             tAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTg                                                                                                                                        >  2:1599654/1‑150 (MQ=255)
             tAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTg                                                                                                                                        >  2:413056/1‑150 (MQ=255)
             tAAACATTTTGTTTTGTGCGGGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCACTGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTg                                                                                                                                        <  2:308824/150‑1 (MQ=255)
                aCATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAg                                                                                                                                     <  2:1571651/150‑1 (MQ=255)
                aCATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAg                                                                                                                                     <  2:1676303/150‑1 (MQ=255)
                aCATTTTGTTGTGTGCGTTGGAACAGCCTTAAGGTGTAAAGGGGGATGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGATGTATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagt                                                                                                                                     <  1:1171268/150‑3 (MQ=255)
                  aTTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagttt                                                                                                                                   <  2:663540/150‑5 (MQ=255)
                   ttttgttttgTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATa                                                                                                                                  <  2:392429/150‑1 (MQ=255)
                     ttgttttgtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATaaa                                                                                                                                >  1:91706/1‑150 (MQ=255)
                       gttttgtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATg                                                                                                                              <  1:1129490/150‑1 (MQ=255)
                             tgCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCttt                                                                                                                        >  2:209772/1‑150 (MQ=255)
                             tgCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCttt                                                                                                                        >  1:1011317/1‑150 (MQ=255)
                              gCGTGGGAACAGCCTTTAGGTTTAAAGGGGGATGTGGAAATAGCAATGAGGAGTATCCGCAAGAATACTCGCCGCTTTACCACAACGTGGATTAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCtttt                                                                                                                       <  2:181347/150‑1 (MQ=255)
                              gCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCtttt                                                                                                                       <  1:809197/150‑1 (MQ=255)
                               cGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTa                                                                                                                      >  2:569426/1‑150 (MQ=255)
                                 tGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACa                                                                                                                    <  2:660928/150‑1 (MQ=255)
                                 ggggAACAGCCGTAAGGTGTAACGGGGGCGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACa                                                                                                                    <  1:947856/149‑1 (MQ=255)
                                  gggAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAg                                                                                                                   <  1:816316/150‑1 (MQ=255)
                                    gAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCAAAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTATTACAGCg                                                                                                                 >  1:316165/1‑150 (MQ=255)
                                       cAGCCTTAAGTTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAATAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGc                                                                                                              <  2:105787/150‑1 (MQ=255)
                                        aGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCt                                                                                                             >  1:1037421/1‑150 (MQ=255)
                                         gCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGAGGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCtt                                                                                                            <  1:561900/150‑1 (MQ=255)
                                          ccTTAATGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTa                                                                                                           <  2:1550527/150‑1 (MQ=255)
                                          ccTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTa                                                                                                           >  1:905233/1‑150 (MQ=255)
                                           cTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTTGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACGCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAt                                                                                                          >  2:266531/1‑150 (MQ=255)
                                           cTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCCTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAt                                                                                                          >  2:1343086/1‑150 (MQ=255)
                                            ttAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAtt                                                                                                         <  2:614351/150‑1 (MQ=255)
                                            ttAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGAAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATTCGCTTATACAGCGGGCTTAtt                                                                                                         >  1:499685/1‑150 (MQ=255)
                                              aaGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTc                                                                                                       <  2:894799/150‑1 (MQ=255)
                                              aaGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTc                                                                                                       <  2:91706/150‑1 (MQ=255)
                                               aGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCa                                                                                                      >  2:643544/1‑150 (MQ=255)
                                                     aaaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGTGATAACTGTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtctt                                                                                                >  2:1111087/1‑150 (MQ=255)
                                                     aaaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtctt                                                                                                >  2:1383667/1‑150 (MQ=255)
                                                      aaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTc                                                                                                       >  2:923296/1‑142 (MQ=255)
                                                      aaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCAAAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtcttc                                                                                               <  2:153937/150‑1 (MQ=255)
                                                      aaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTAGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtcttc                                                                                               >  1:1016144/1‑150 (MQ=255)
                                                          gggAGGTTGAAATAGCAATTAGGAGTATCAGAAAGAATACTCTCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCt                                                                                           <  1:1891584/150‑1 (MQ=255)
                                                           ggAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCtt                                                                                          >  1:907617/1‑150 (MQ=255)
                                                              ggaggAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTATACCACAACCTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGACTTGAAGATAAAAGCGCTTAAACAGCGGGCTTATTACAGCTCTACTCCATccc                                                                                       >  2:763190/4‑149 (MQ=255)
                                                               gTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCgg                                                                                      >  2:1808619/1‑150 (MQ=255)
                                                                tGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTTCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGt                                                                                     >  2:855239/1‑150 (MQ=255)
                                                                   aaaTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAg                                                                                  <  1:818369/150‑1 (MQ=255)
                                                                    aaatgCAATGAGTAGTATCAGCAAGAATACTCGCCGCTTTAGCAGAACGTGGATGAGAGGTATGATAAACTCAAGTCAGAGATAACTCTGGCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTGTGCTTCCGGTAAgg                                                                                 <  1:125936/146‑1 (MQ=255)
                                                                    aaTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAgg                                                                                 <  1:1744304/150‑1 (MQ=255)
                                                                     aTAGCAATGAGGAGTATCAGCAAGAATACTAGCCGCATTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCGTATTTCAGCTCTTCTGCTTCCGGTAAGGt                                                                                <  1:260796/150‑1 (MQ=255)
                                                                      tAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTc                                                                               >  1:619876/1‑150 (MQ=255)
                                                                      tAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTAAGCTCTTCTGCTTCCGGTAAGGTc                                                                               >  2:1097614/1‑150 (MQ=255)
                                                                         cAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACg                                                                            <  2:718121/150‑1 (MQ=255)
                                                                          aaTGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGt                                                                           <  1:990535/150‑1 (MQ=255)
                                                                            tGAGGAGTAGCAGCAAGTCTACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTc                                                                         <  2:1417554/150‑1 (MQ=255)
                                                                             gaggagTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCa                                                                        <  2:133906/150‑1 (MQ=255)
                                                                               ggagTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGc                                                                      <  1:1554824/150‑1 (MQ=255)
                                                                                        gCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCAAGTGCAGCTCAAGAATa                                                             >  1:1696583/1‑150 (MQ=255)
                                                                                         cAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAg                                                            >  2:1333206/1‑150 (MQ=255)
                                                                                         cAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATCCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAg                                                            <  2:1354478/150‑1 (MQ=255)
                                                                                            gAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGaaa                                                         <  1:1627766/150‑1 (MQ=255)
                                                                                                  tCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGc                                                   <  2:185512/150‑1 (MQ=255)
                                                                                                  tCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGc                                                   <  1:413056/150‑1 (MQ=255)
                                                                                                   cgccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGcc                                                  >  1:491332/1‑150 (MQ=255)
                                                                                                   cgccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCGTCTGCTTCAGGTAAGGTCACGTTCAGATCAAGAATAGAAATATCGcc                                                  >  1:98407/1‑150 (MQ=255)
                                                                                                      cgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATc                                               >  2:445782/1‑150 (MQ=255)
                                                                                                           tACCACCACGGGGATGAGAGGGAGCTAAAACTCAAGGCAGAGATACCGCTGCCGTGAAGATATATGCGCTTTTACAGAGGGCTGATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAATAATAGAAATATCGCCATCttttt                                          <  2:280579/150‑1 (MQ=255)
                                                                                                             ccACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTgg                                        >  2:322593/1‑149 (MQ=255)
                                                                                                             ccACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCGTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCAGGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGc                                        >  2:286607/1‑150 (MQ=255)
                                                                                                             ccACAACGTGGATGAGAGGGAGGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGAGCATTTACAGCGGGCATATTTCAGCTCTTCTGCTTCCCGTAAGTTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGc                                        >  2:122600/1‑150 (MQ=255)
                                                                                                              cacaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCCGCTCATCTGCGTCCGGTAAGGTCCCGTGCCGCTCAAGAATAGAAATTTCGCCATCTTTTTGcg                                       >  2:311982/1‑149 (MQ=255)
                                                                                                                caACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCATGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCATCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCa                                     >  1:919231/1‑150 (MQ=255)
                                                                                                                    gTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCTGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCt                                 <  1:496715/150‑1 (MQ=255)
                                                                                                                     tGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTATTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAAAATCGCCATATTTTTGCTCAAGCTg                                >  2:1315331/1‑150 (MQ=255)
                                                                                                                      ggATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGt                               >  2:981581/1‑150 (MQ=255)
                                                                                                                        aTGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTAc                             >  2:1090877/1‑150 (MQ=255)
                                                                                                                        aTGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTAc                             >  1:554193/1‑150 (MQ=255)
                                                                                                                         tGAGAGGGATGAAAAACTCAAGGCATAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGATTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACg                            <  1:1104378/150‑1 (MQ=255)
                                                                                                                          gagaGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACgg                           <  1:1419304/150‑1 (MQ=255)
                                                                                                                             agGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTa                        <  1:1719972/150‑1 (MQ=255)
                                                                                                                             agGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTa                        <  2:624324/150‑1 (MQ=255)
                                                                                                                                gATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCa                     <  1:1448346/150‑1 (MQ=255)
                                                                                                                                gATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTAAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCa                     >  1:1456181/1‑150 (MQ=255)
                                                                                                                                 aTGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCTAGATTAGTAATATGGCCATCTTTTTGATCAAGCTGTACGGTTACCAt                    >  1:357477/1‑150 (MQ=255)
                                                                                                                                  tGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCAtc                   >  2:931386/1‑150 (MQ=255)
                                                                                                                                   gAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCAtct                  >  1:1181617/1‑150 (MQ=255)
                                                                                                                                   gAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCAtct                  >  2:356344/1‑150 (MQ=255)
                                                                                                                                           cAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCa          >  1:728804/1‑150 (MQ=255)
                                                                                                                                           cAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCa          >  1:1788290/1‑150 (MQ=255)
                                                                                                                                            aaGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCaa         >  2:1585122/1‑150 (MQ=255)
                                                                                                                                            aaGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCaa         >  2:855729/1‑150 (MQ=255)
                                                                                                                                             aGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAt        >  1:1688124/1‑150 (MQ=255)
                                                                                                                                              ggCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGATATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAtt       >  1:335563/1‑150 (MQ=255)
                                                                                                                                              ggCAGAGATAACTCTGCCTTGAAGATAAATGCGATTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAtt       <  1:1374001/150‑1 (MQ=255)
                                                                                                                                                  gagaTAACTCTGCCTTGAAGATAAATGCGATTTTACAGCGGGCTTTTTTCAGCTCTTCTTCTTCCGGTAAGGTCACGTTCAGCTCAAGTATTGAAATATCGCCATCTTTTTGCTCTAGCTGTACGGTTACCTTCTCAGGATCAATTTGt  <  1:763190/150‑2 (MQ=255)
                                                                                                                                                   agaTAACTCTGCCTTGAAGATAACTGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTAc  <  2:452741/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTAC  >  NC_000913/1224090‑1224386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: