Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,224,242 A→T 5.0% intergenic (+335/+37) ycgI → / ← minE pseudogene/cell division topological specificity factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,224,2420AT 5.0% 355.5 / 6.1 119intergenic (+335/+37)ycgI/minEpseudogene/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base A (60/52);  new base T (2/4);  total (62/57)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01

Only 100 of 125 total aligned reads displayed.

CAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATT  >  NC_000913/1224093‑1224391
                                                                                                                                                     |                                                                                                                                                     
cAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTTAAGGGGGAGGTGGAAATAGCAATGAGGAGTCTCAGCAAGAATACTCGCCGCATTACCACAACGTGGATGAGAGGGATGAAAAACTCTAGGAAGAGATaa                                                                                                                                                       >  2:338259/1‑150 (MQ=255)
cAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATaa                                                                                                                                                       >  2:1448346/1‑150 (MQ=255)
cAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATaa                                                                                                                                                       >  2:600550/1‑150 (MQ=255)
cAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATaa                                                                                                                                                       >  1:410398/1‑150 (MQ=255)
        cATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCt                                                                                                                                               >  1:1166924/1‑150 (MQ=255)
         aTAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCtt                                                                                                                                              <  1:1132361/150‑1 (MQ=255)
          tAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTg                                                                                                                                             >  2:413056/1‑150 (MQ=255)
          tAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTg                                                                                                                                             <  1:612850/150‑1 (MQ=255)
          tAAACATTTTGTTTTGTGCGGGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCACTGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTg                                                                                                                                             <  2:308824/150‑1 (MQ=255)
            aaCATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGag                                                                                                                                           <  2:734601/150‑2 (MQ=255)
             aCATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAg                                                                                                                                          <  2:1571651/150‑1 (MQ=255)
             aCATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAg                                                                                                                                          <  2:1676303/150‑1 (MQ=255)
             aCATTTTGTTGTGTGCGTTGGAACAGCCTTAAGGTGTAAAGGGGGATGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGATGTATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagt                                                                                                                                          <  1:1171268/150‑3 (MQ=255)
              cATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGa                                                                                                                                         <  1:652992/150‑1 (MQ=255)
               aTTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagttt                                                                                                                                        <  2:663540/150‑5 (MQ=255)
               aTTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGAt                                                                                                                                        >  2:457725/1‑150 (MQ=255)
                  ttgttttgtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATaaa                                                                                                                                     >  1:91706/1‑150 (MQ=255)
                          tgCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCttt                                                                                                                             >  1:1011317/1‑150 (MQ=255)
                          tgCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCttt                                                                                                                             >  2:209772/1‑150 (MQ=255)
                           gCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCtttt                                                                                                                            <  1:809197/150‑1 (MQ=255)
                              tGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACa                                                                                                                         <  2:660928/150‑1 (MQ=255)
                               gggAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAg                                                                                                                        <  1:816316/150‑1 (MQ=255)
                                 gAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCg                                                                                                                      <  2:623160/150‑1 (MQ=255)
                                    cAGCCTTAAGTTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAATAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGc                                                                                                                   <  2:105787/150‑1 (MQ=255)
                                     aGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCt                                                                                                                  >  1:1037421/1‑150 (MQ=255)
                                      gCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCtt                                                                                                                 >  2:1660954/1‑150 (MQ=255)
                                       ccTTAATGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTa                                                                                                                <  2:1550527/150‑1 (MQ=255)
                                        cTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCCTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAt                                                                                                               >  2:1343086/1‑150 (MQ=255)
                                         ttAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAtt                                                                                                              <  2:614351/150‑1 (MQ=255)
                                         ttAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGAAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATTCGCTTATACAGCGGGCTTAtt                                                                                                              >  1:499685/1‑150 (MQ=255)
                                           aaGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTc                                                                                                            <  2:91706/150‑1 (MQ=255)
                                           aaGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTc                                                                                                            <  2:894799/150‑1 (MQ=255)
                                             ggTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACACCGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAg                                                                                                          >  2:362166/1‑150 (MQ=255)
                                                  aaaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGTGATAACTGTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtctt                                                                                                     >  2:1111087/1‑150 (MQ=255)
                                                  aaaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtctt                                                                                                     >  2:1383667/1‑150 (MQ=255)
                                                   aaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTc                                                                                                            <  1:923296/142‑1 (MQ=255)
                                                   aaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTc                                                                                                            >  2:923296/1‑142 (MQ=255)
                                                   aaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCAAAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtcttc                                                                                                    <  2:153937/150‑1 (MQ=255)
                                                   aaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTAGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtcttc                                                                                                    >  1:1016144/1‑150 (MQ=255)
                                                       gggAGGTTGAAATAGCAATTAGGAGTATCAGAAAGAATACTCTCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCt                                                                                                <  1:1891584/150‑1 (MQ=255)
                                                        ggAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCtt                                                                                               >  1:907617/1‑150 (MQ=255)
                                                            gTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCgg                                                                                           >  2:1808619/1‑150 (MQ=255)
                                                             tGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTTCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGt                                                                                          >  2:855239/1‑150 (MQ=255)
                                                                aaaTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAg                                                                                       <  1:818369/150‑1 (MQ=255)
                                                                 aaTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAgg                                                                                      >  1:1589346/1‑150 (MQ=255)
                                                                  aTAGCAATGAGGAGTATCAGCAAGAATACTAGCCGCATTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCGTATTTCAGCTCTTCTGCTTCCGGTAAGGt                                                                                     <  1:260796/150‑1 (MQ=255)
                                                                   tAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTc                                                                                    >  1:619876/1‑150 (MQ=255)
                                                                   tAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTAAGCTCTTCTGCTTCCGGTAAGGTc                                                                                    >  2:1097614/1‑150 (MQ=255)
                                                                     gCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGAAAACACTGCCTTGAAGATATCTGCGCGTTTACAGCGGGCTTATTTGAGCTATTCTGCTGCCGGGAATGTCAc                                                                                  >  2:295896/1‑150 (MQ=255)
                                                                      cAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACg                                                                                 <  2:718121/150‑1 (MQ=255)
                                                                       aaTGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGt                                                                                <  1:990535/150‑1 (MQ=255)
                                                                         tGAGGAGTAGCAGCAAGTCTACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTc                                                                              <  2:1417554/150‑1 (MQ=255)
                                                                          gaggagTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCa                                                                             <  2:133906/150‑1 (MQ=255)
                                                                            ggagTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGc                                                                           <  1:1554824/150‑1 (MQ=255)
                                                                                     gCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCAAGTGCAGCTCAAGAATa                                                                  >  1:1696583/1‑150 (MQ=255)
                                                                                      cAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAg                                                                 <  2:1864516/150‑1 (MQ=255)
                                                                                      cAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAg                                                                 >  2:1333206/1‑150 (MQ=255)
                                                                                      cAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATCCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAg                                                                 <  2:1354478/150‑1 (MQ=255)
                                                                                         gAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGaaa                                                              <  1:1627766/150‑1 (MQ=255)
                                                                                               tCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGc                                                        <  1:413056/150‑1 (MQ=255)
                                                                                               tCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGc                                                        <  2:185512/150‑1 (MQ=255)
                                                                                                cgccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGcc                                                       >  2:1016494/1‑150 (MQ=255)
                                                                                                cgccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCGTCTGCTTCAGGTAAGGTCACGTTCAGATCAAGAATAGAAATATCGcc                                                       >  1:98407/1‑150 (MQ=255)
                                                                                                   cgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATc                                                    >  2:445782/1‑150 (MQ=255)
                                                                                                        tACCACCACGGGGATGAGAGGGAGCTAAAACTCAAGGCAGAGATACCGCTGCCGTGAAGATATATGCGCTTTTACAGAGGGCTGATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAATAATAGAAATATCGCCATCttttt                                               <  2:280579/150‑1 (MQ=255)
                                                                                                          ccACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTgg                                             >  2:322593/1‑149 (MQ=255)
                                                                                                          ccACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCGTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCAGGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGc                                             >  2:286607/1‑150 (MQ=255)
                                                                                                          ccACAACGTGGATGAGAGGGAGGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGAGCATTTACAGCGGGCATATTTCAGCTCTTCTGCTTCCCGTAAGTTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGc                                             >  2:122600/1‑150 (MQ=255)
                                                                                                             caACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCa                                          <  1:209772/150‑1 (MQ=255)
                                                                                                             caACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCATGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCATCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCa                                          >  1:919231/1‑150 (MQ=255)
                                                                                                                 gTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCTGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCt                                      <  1:496715/150‑1 (MQ=255)
                                                                                                                  tGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTATTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAAAATCGCCATATTTTTGCTCAAGCTg                                     >  2:1315331/1‑150 (MQ=255)
                                                                                                                   ggATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGt                                    >  2:981581/1‑150 (MQ=255)
                                                                                                                     aTGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTAc                                  >  1:554193/1‑150 (MQ=255)
                                                                                                                      tGAGAGGGATGAAAAACTCAAGGCATAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGATTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACg                                 <  1:1104378/150‑1 (MQ=255)
                                                                                                                       gagaGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACgg                                <  1:1419304/150‑1 (MQ=255)
                                                                                                                          agGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTa                             <  1:1719972/150‑1 (MQ=255)
                                                                                                                          agGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTa                             <  2:624324/150‑1 (MQ=255)
                                                                                                                            ggATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTAcc                           >  1:895406/1‑150 (MQ=255)
                                                                                                                             gATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCa                          <  1:1448346/150‑1 (MQ=255)
                                                                                                                              aTGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCTAGATTAGTAATATGGCCATCTTTTTGATCAAGCTGTACGGTTACCAt                         >  1:357477/1‑150 (MQ=255)
                                                                                                                               tGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCAtc                        >  2:931386/1‑150 (MQ=255)
                                                                                                                                gAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCAtct                       >  2:356344/1‑150 (MQ=255)
                                                                                                                                gAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCAtct                       >  1:1181617/1‑150 (MQ=255)
                                                                                                                                   aaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAg                    <  1:1312965/150‑1 (MQ=255)
                                                                                                                                      cTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGAt                 <  1:1707754/150‑1 (MQ=255)
                                                                                                                                        cAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCa               >  1:510462/1‑150 (MQ=255)
                                                                                                                                        cAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCa               >  1:1788290/1‑150 (MQ=255)
                                                                                                                                        cAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCa               >  1:728804/1‑150 (MQ=255)
                                                                                                                                         aaGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCaa              >  2:1585122/1‑150 (MQ=255)
                                                                                                                                         aaGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCaa              >  2:855729/1‑150 (MQ=255)
                                                                                                                                          aGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAt             >  1:1688124/1‑150 (MQ=255)
                                                                                                                                           ggCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGATATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAtt            >  1:335563/1‑150 (MQ=255)
                                                                                                                                           ggCAGAGATAACTCTGCCTTGAAGATAAATGCGATTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAtt            <  1:1374001/150‑1 (MQ=255)
                                                                                                                                                agaTAACTCTGCCTTGAAGATAACTGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTAc       <  2:452741/150‑1 (MQ=255)
                                                                                                                                                   tAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTa    >  1:1493081/1‑150 (MQ=255)
                                                                                                                                                   tAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTa    <  2:1100375/150‑1 (MQ=255)
                                                                                                                                                   tAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTa    <  2:1872885/150‑1 (MQ=255)
                                                                                                                                                    aaCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCGCAGGATCAATTTGTACGTAt   >  2:1679413/1‑150 (MQ=255)
                                                                                                                                                     aCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTAtt  <  2:907617/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATT  >  NC_000913/1224093‑1224391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: