Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,356,057 T→G 5.9% N352H (AAC→CAC)  sapA ← antimicrobial peptide transport ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,356,0570TG 5.9% 298.1 / 4.8 107N352H (AAC→CAC) sapAantimicrobial peptide transport ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (47/51);  new base G (4/3);  total (52/55)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

CTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAA  >  NC_000913/1355908‑1356206
                                                                                                                                                     |                                                                                                                                                     
cTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGtt                                                                                                                                                       <  1:1687490/150‑1 (MQ=255)
  gAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGt                                                                                                                                                     <  2:426637/150‑1 (MQ=255)
    aTCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCa                                                                                                                                                   >  2:1897638/1‑150 (MQ=255)
       aGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCAGGATTGTATAGTGTAATTTTATCCTCGTTGTCATAg                                                                                                                                                >  2:1432001/1‑150 (MQ=255)
        gTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTGGTCATAgg                                                                                                                                               >  1:1384930/1‑150 (MQ=255)
           cGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGccc                                                                                                                                            >  2:1529288/1‑150 (MQ=255)
            ggCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCa                                                                                                                                           <  1:1413906/150‑1 (MQ=255)
             gaaGTTTTCAGTGGACTGGGGTTACACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCAc                                                                                                                                          <  1:351386/148‑1 (MQ=255)
               aggtttCAGTGGACTGGGGTTCCACGCCTGCGAAGGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGGTCGCGCGAGAGCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGa                                                                                                                                        <  1:306646/147‑1 (MQ=255)
               aGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGa                                                                                                                                        <  1:1060232/150‑1 (MQ=255)
                   ttCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGgcg                                                                                                                                    >  2:1517593/1‑150 (MQ=255)
                    acAGTGGACTGGGGGTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTGGGCCTATGACAACGAGgcta                                                                                                                                   <  2:1466719/149‑3 (MQ=255)
                     cAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGgcgcg                                                                                                                                  <  1:745847/150‑1 (MQ=255)
                     cAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGgcgcg                                                                                                                                  >  2:1735099/1‑150 (MQ=255)
                     cAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTACGCGCGATTTCGCCGTATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGTCCCACCAcgcggg                                                                                                                                  >  2:1040788/1‑148 (MQ=255)
                       gTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGcgcgcg                                                                                                                                <  1:1344021/150‑1 (MQ=255)
                       gTGGACTGGGGGTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTGCGCGCGATTGCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGcgcgcg                                                                                                                                <  2:985299/150‑1 (MQ=255)
                         ggACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCACCTGTTCGCGCGAGGTCGCCGGATGGTAGTCAGGACTTTTAGCCTCGTGGTCATAGGCCCACGAGGCGCGCGGt                                                                                                                              >  1:227396/1‑150 (MQ=255)
                             tGGCGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCCACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTATAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTaaaa                                                                                                                          <  1:85581/150‑1 (MQ=255)
                              ggggTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTCGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAAt                                                                                                                         >  2:502124/1‑150 (MQ=255)
                               gggTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCACCTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATa                                                                                                                        >  1:1726162/1‑150 (MQ=255)
                                ggTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATATGCCCACGAGGCGCGCGGTAAAATag                                                                                                                       <  2:1457916/150‑1 (MQ=255)
                                     cACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCCACGACTTCAACTGTTCGCGCGATTTCGCCGGAGTGGATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGgcc                                                                                                                  >  2:340210/1‑150 (MQ=255)
                                      atgCCGGCGAACGTGTGGGCACCCACAGTATCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGgccg                                                                                                                 <  1:1271485/148‑1 (MQ=255)
                                          ccgCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGt                                                                                                             >  1:1356771/3‑150 (MQ=255)
                                           tGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGtt                                                                                                            >  2:747454/1‑150 (MQ=255)
                                           tGCGAACGTGTGGGCACCCACAGTATCAGCGTTTAATTTTCCAGCCCCAACGCCTTCAACTTTTCGCGAGAATTCGCCGGAATGTATTCAGTAAATTTAGCCTCGTAGTCATACGCCCACGAGGCGCTCGGTAAAATTGCGGCCGCCttt                                                                                                            >  2:91393/1‑150 (MQ=255)
                                            gCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCACCGttt                                                                                                           >  1:1486648/1‑150 (MQ=255)
                                             cGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTc                                                                                                          <  2:543074/150‑1 (MQ=255)
                                             cGAACGTGTGGGCACCCACAGTTTAAGCGTTAAATTTTCCAGCCCCATCGACTTCAACTGTTCGCGCGATTTCGCCGGTTTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTc                                                                                                          <  2:781410/150‑1 (MQ=255)
                                               aaCGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAg                                                                                                        <  1:131589/150‑1 (MQ=255)
                                               aaCGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAg                                                                                                        <  2:423299/150‑1 (MQ=255)
                                                aCGTGTGGGCACCCTCAGTTGCAGCGTTAAATTATCCAGCCCCAACGATTTCATCTGTTAGCGCGATTTCGCCGTATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCatc                                                                                                       <  2:336648/150‑3 (MQ=255)
                                                aCGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAAGGACTTCAAATGTTCGCGCGATTTCGCCGGTTTGTATTCAGTAATTTTAGCCTCGTTGTAATAGGCACACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGc                                                                                                       <  1:436464/150‑1 (MQ=255)
                                                     tgGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACCGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTAc                                                                                                  <  2:1295130/150‑1 (MQ=255)
                                                      gggCACCCACAGTTTCAGCGTTAAATTTACCAGCCCCAACGACTTCAACTGTTAGCGCGATTTCGACGGATTTTATTCAGTTATTTTAGCCTCGTTGTCCTGGGCCCCCGGGGCGCGCGGTTAAATAGAGGCCGGCGTTAAAGCCGTAcc                                                                                                 >  2:121439/1‑150 (MQ=255)
                                                       ggCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAATATAGAGGCCGCCGTTTCAGCCGTACCa                                                                                                >  2:748761/1‑150 (MQ=255)
                                                        gCACCCACAGTTTCAGCGTTAAATTTACCAGCCACAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCat                                                                                               <  2:250001/150‑1 (MQ=255)
                                                         cACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCata                                                                                              <  2:1591703/150‑1 (MQ=255)
                                                          aCCCACAGTTTCAGCGTTAAATTTTCCAGCCCCACCGACTTCAACAGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATATGCCCACTAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCATCCGTAACataa                                                                                             >  2:1199822/1‑150 (MQ=255)
                                                           cccACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCataat                                                                                            >  1:202853/1‑150 (MQ=255)
                                                           cccACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCataat                                                                                            >  2:1518635/1‑150 (MQ=255)
                                                            ccACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCataata                                                                                           <  2:1181628/150‑1 (MQ=255)
                                                                 gTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATgg                                                                                      <  2:1108467/150‑1 (MQ=255)
                                                                    tCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGAtt                                                                                   <  2:565753/150‑1 (MQ=255)
                                                                    tCAGCGTTAAATATTCCAGCACGAACGACTTAAAATGTTCGCGGGATTTCGGCCGATTTTATTCAGTAAATTTAGCCGCGTTGTGATAGGCACACGAGGGGCGCGGTAAAATTGAGGCCGGCGTTTCAGCCGTACCATAATAGATGGAtt                                                                                   <  2:926764/150‑1 (MQ=255)
                                                                     cAGCGTTAAATTTTCCAGCCCCAACGACTTCTACTGTTAGCTCGATTTCGCCGGATTTTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTg                                                                                  <  1:1040788/150‑1 (MQ=255)
                                                                     cAGCGTTAAAATTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTg                                                                                  >  2:58936/1‑150 (MQ=255)
                                                                       gCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCa                                                                                >  1:636504/1‑150 (MQ=255)
                                                                        cGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCAt                                                                               >  2:456419/1‑150 (MQ=255)
                                                                         gTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATc                                                                              >  1:1390872/1‑150 (MQ=255)
                                                                            aaaTTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAgg                                                                           >  1:1201163/1‑150 (MQ=255)
                                                                             aaTTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGCCGCGCGGTAAAATAGAGGCCGCCCTTTCAGCCGTACCATAATAGATGGATTGCATCAGgc                                                                          >  2:514257/1‑150 (MQ=255)
                                                                              aTTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGgcg                                                                         >  2:19190/1‑150 (MQ=255)
                                                                                tttCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCt                                                                       >  1:1072087/1‑150 (MQ=255)
                                                                                   ccAGCCCCACCGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGGTGTCTTAGGCCCACGAGGCGGGCGGTAAAATATAGGCAGCCGGTTCCGCCGTACCATAATAGATGGAGTGCATCCGgcgagggt                                                                    >  2:278005/1‑145 (MQ=255)
                                                                                   ccAGCCCCAACGACTTCATCTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGt                                                                    <  2:1883951/150‑1 (MQ=255)
                                                                                   ccAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGt                                                                    >  2:701825/1‑150 (MQ=255)
                                                                                    cAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGtt                                                                   >  1:402737/1‑150 (MQ=255)
                                                                                       ccccACCGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGttatt                                                                <  2:1200846/150‑1 (MQ=255)
                                                                                       ccccAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGttatt                                                                <  1:200096/150‑1 (MQ=255)
                                                                                       ccccAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGACGACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCTTAATAGATGGATTGCATCAGGCGCTGGttatt                                                                <  1:74941/150‑1 (MQ=255)
                                                                                         ccAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTaa                                                              >  2:1834512/1‑150 (MQ=255)
                                                                                            aCGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCg                                                           <  1:532306/150‑1 (MQ=255)
                                                                                              gACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGcc                                                         <  2:1012366/150‑1 (MQ=255)
                                                                                              gACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTCGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGcc                                                         <  2:603693/150‑1 (MQ=255)
                                                                                               aCTTCAACTGTTCGCGCGAATTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGTTAAAATAGCGGCCGCCCTTTCAGCCGTACCATAATAGATGGATAGCATCAGGCGCTGGTTAGTAAGCGccc                                                        >  2:302150/1‑149 (MQ=255)
                                                                                                cTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAg                                                       >  1:985423/1‑150 (MQ=255)
                                                                                                cTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAg                                                       >  2:1610188/1‑150 (MQ=255)
                                                                                                 ttCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGt                                                      >  2:1317764/1‑150 (MQ=255)
                                                                                                  tCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTg                                                     >  1:697236/1‑150 (MQ=255)
                                                                                                   cAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGc                                                    >  1:425981/1‑150 (MQ=255)
                                                                                                        gTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAgcg                                               >  2:48896/1‑150 (MQ=255)
                                                                                                        gTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCAGGTTATTAATCGCCAGAGCCAgcc                                               >  1:426000/1‑149 (MQ=255)
                                                                                                           cgcgcgATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCAt                                            >  2:892696/1‑150 (MQ=255)
                                                                                                           cgcgcgATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCAt                                            <  2:925534/150‑1 (MQ=255)
                                                                                                               cgATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCg                                        <  2:1765761/150‑1 (MQ=255)
                                                                                                                  tttCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGAc                                     <  2:1729531/150‑1 (MQ=255)
                                                                                                                     cGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGc                                  >  2:1529120/1‑150 (MQ=255)
                                                                                                                      gCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCg                                 >  1:1651759/1‑150 (MQ=255)
                                                                                                                       ccGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCgg                                >  1:879423/1‑150 (MQ=255)
                                                                                                                          gATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGat                             <  1:1176740/150‑1 (MQ=255)
                                                                                                                          gATTGTATTCAGTAATTTTAGCCGCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGat                             <  1:184008/150‑1 (MQ=255)
                                                                                                                            ttGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGATGCCGCCGTTTCAGCCGTACCATAATAGATTGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGatta                           <  2:1512442/150‑1 (MQ=255)
                                                                                                                            ttGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGatta                           <  2:1910791/150‑1 (MQ=255)
                                                                                                                            ttGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGatta                           >  1:1113617/1‑150 (MQ=255)
                                                                                                                               tATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGGTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTAttt                        <  2:895549/150‑1 (MQ=255)
                                                                                                                                aTTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGCGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTa                       <  1:1529288/150‑1 (MQ=255)
                                                                                                                                  tCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAgc                     <  2:257288/150‑1 (MQ=255)
                                                                                                                                  tCAGTAATTTTAGCCTCGTTGACATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAgc                     <  1:1006099/150‑1 (MQ=255)
                                                                                                                                        aTTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGt               <  1:167096/150‑1 (MQ=255)
                                                                                                                                         cgcgcgCCTCGTGGGCCTATGACAACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGGACCATTATAGATGGATTGCATCAGGCGCGGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGAGGATTTAGCGGCGGtt              >  1:937439/6‑150 (MQ=255)
                                                                                                                                            tAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGTTTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTgg           <  1:1367926/150‑1 (MQ=255)
                                                                                                                                             cgCCTCGTGGGCCTATGACAACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCCGCCGTGCCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATGTAGCGGCGGGGTGGc          >  2:653248/2‑150 (MQ=255)
                                                                                                                                              gCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCg         <  1:1028513/150‑1 (MQ=255)
                                                                                                                                               ccTCGTTGTCATCGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATACTAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCgg        <  1:1897638/150‑1 (MQ=255)
                                                                                                                                                 tCGTGGTCATAGGCCCCCGCGGCGCGCGGGAAAATCGAGGCCGCCGTTGCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGCTATTCCTCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGgtg      <  2:1240180/150‑1 (MQ=255)
                                                                                                                                                 gcGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATACTAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGgtg      <  1:114765/149‑1 (MQ=255)
                                                                                                                                                   gTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGtt    >  2:4617/1‑150 (MQ=255)
                                                                                                                                                     tGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAAACGCCAGTGCCAGCGCATGGCGGACCGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTaa  >  2:1699084/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAA  >  NC_000913/1355908‑1356206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: