Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 756,886 T→G L327R (CTG→CGG)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,8860TG94.3% 378.2 / 6.4 124L327R (CTG→CGG) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (2/5);  new base G (51/66);  total (53/71)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 124 total aligned reads displayed.

CGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGC  >  NC_000913/756739‑757031
                                                                                                                                                   |                                                                                                                                                 
cGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACCCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGc                                                                                                                                                 >  1:2044997/1‑150 (MQ=255)
 gAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGcc                                                                                                                                                >  1:1524925/1‑150 (MQ=255)
     gCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGt                                                                                                                                            >  1:1791056/1‑150 (MQ=255)
      ccAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAAACCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATGACCTGGGTAAAGAACGTCTCGAATCCCCTCGGCCGGGtt                                                                                                                                           >  1:1433581/1‑149 (MQ=255)
         aaccccTGGGGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGCAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAAGTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATcc                                                                                                                                        <  1:833780/146‑1 (MQ=255)
              cTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAg                                                                                                                                   <  1:1728542/150‑1 (MQ=255)
                ggCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCt                                                                                                                                 >  2:1251809/1‑150 (MQ=255)
                  cGGGCCGTGACGTGGGTGCGCGTTCCATCATGATCGAAATCCGTGAAGGCCGCGGCTGGGATGGGCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCttt                                                                                                                               <  2:1328362/150‑1 (MQ=255)
                     gCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTccc                                                                                                                            >  1:1185993/1‑150 (MQ=255)
                      ccGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCg                                                                                                                           <  2:469780/150‑1 (MQ=255)
                      ccGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCg                                                                                                                           <  1:1581623/150‑1 (MQ=255)
                      ccGGGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGGGAAGGTCGCGGCTGTGTTGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCg                                                                                                                           <  2:326784/150‑1 (MQ=255)
                        gTGACGTGGTTGCGCGTTCGATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTa                                                                                                                         <  1:1110125/150‑1 (MQ=255)
                        gTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTa                                                                                                                         <  2:71476/150‑1 (MQ=255)
                              tGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCg                                                                                                                   >  1:291958/1‑150 (MQ=255)
                               ggTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGc                                                                                                                  <  1:1594686/150‑1 (MQ=255)
                                gattcgcgTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCt                                                                                                                 <  2:337676/146‑1 (MQ=255)
                                gTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGcg                                                                                                                 >  1:1498123/1‑149 (MQ=255)
                                    cgcgTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACg                                                                                                             >  1:2173561/1‑150 (MQ=255)
                                      cgTGCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGTGAAACTGAAACTCGATCTCCTGGGTAAAGAAGTTCTCGAATTCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTc                                                                                                           <  1:371067/150‑1 (MQ=255)
                                          ccATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGGTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATc                                                                                                       <  1:733675/150‑1 (MQ=255)
                                          ccATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACCCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATc                                                                                                       >  1:863573/1‑150 (MQ=255)
                                          ccATCATGATCGAAATCCGGGAAGGTCGCGGCTGTGATGGTCCGGGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCAGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATc                                                                                                       <  2:1277457/150‑1 (MQ=255)
                                            atcatGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCg                                                                                                     >  2:376885/1‑150 (MQ=255)
                                             tcatGATCGCAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGACCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCgg                                                                                                    >  1:1757710/1‑150 (MQ=255)
                                             tcatGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCgg                                                                                                    >  1:770701/1‑150 (MQ=255)
                                             tcatGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCgg                                                                                                    >  1:621552/1‑150 (MQ=255)
                                                  aTCGATATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGaaa                                                                                               <  1:1467893/150‑1 (MQ=255)
                                                  aTCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGaat                                                                                               >  1:520190/1‑149 (MQ=255)
                                                    cGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAaga                                                                                             >  2:42189/1‑150 (MQ=255)
                                                     gAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGTCCTGGTATCCTGTAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAagag                                                                                            <  2:269520/150‑1 (MQ=255)
                                                      aaaTCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGc                                                                                           >  1:820737/1‑150 (MQ=255)
                                                      aaaTCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGcc                                                                                          <  1:1345329/150‑1 (MQ=255)
                                                        aTCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCg                                                                                         <  2:2043760/150‑1 (MQ=255)
                                                        aTCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCg                                                                                         <  1:580123/150‑1 (MQ=255)
                                                            gTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGGCGATCCGGTGCAAGCGCCGATTc                                                                                     >  2:123758/1‑150 (MQ=255)
                                                             tGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTcc                                                                                    <  2:674464/150‑1 (MQ=255)
                                                               aaGGGCGCGGGTGTGAGCGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGTGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTACCCGGCCCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCgg                                                                                  <  2:180140/150‑1 (MQ=255)
                                                                aGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGt                                                                                 <  1:1691569/150‑1 (MQ=255)
                                                                   tCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTAt                                                                              >  2:1670722/1‑150 (MQ=255)
                                                                    cgcgGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCATTCCCGTACCTTCGCGCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATc                                                                             >  1:1370615/1‑150 (MQ=255)
                                                                      cgGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGAGAAAGAGCCGATTCCGGTTATccc                                                                           >  1:799853/1‑150 (MQ=255)
                                                                          tgtgATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAAcc                                                                       <  1:2038769/150‑1 (MQ=255)
                                                                              aTGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTc                                                                   <  1:556461/150‑1 (MQ=255)
                                                                               tGGTCCGTGGGGGTCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCa                                                                  <  2:718022/150‑1 (MQ=255)
                                                                                ggTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCCACCTGTCAc                                                                 >  1:989008/1‑150 (MQ=255)
                                                                                ggTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAc                                                                 <  1:1967992/150‑1 (MQ=255)
                                                                                 gTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCCCCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTAAACTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGAGCAAGAGCCGAGTCCGGTTATCCCAACCTGTCACt                                                                >  1:1617448/1‑150 (MQ=255)
                                                                                 gTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACt                                                                >  2:1310468/1‑150 (MQ=255)
                                                                                    cGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACa                                                             <  1:952901/150‑1 (MQ=255)
                                                                                       gggggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatga                                                          <  1:2103150/150‑1 (MQ=255)
                                                                                         gggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatgatg                                                        >  2:1383137/1‑150 (MQ=255)
                                                                                             cacaCGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCg                                                    <  1:2157795/150‑1 (MQ=255)
                                                                                              acacGCGAATCTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGGCGATCCGGTGAAAGAGCCGATTCCGGGTATCCCAACCTGTCACTACATGATGGGCgg                                                   <  2:2044997/150‑1 (MQ=255)
                                                                                              acacGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGATCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCgg                                                   <  1:1396136/150‑1 (MQ=255)
                                                                                               cacGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTAGAATCCCGTCTGCCGCGTATACTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCCATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGt                                                  >  1:1888490/1‑150 (MQ=255)
                                                                                               cacGCGAAACGGAAACTCGATCACCTTGGGAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCAGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGt                                                  <  1:1928278/150‑1 (MQ=255)
                                                                                                acGCGAAACTGGAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTa                                                 <  2:2142231/150‑1 (MQ=255)
                                                                                                acGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGGCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTa                                                 <  1:1952025/150‑1 (MQ=255)
                                                                                                 cgcgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGCTTATCCCAACCTGTCACAACATGATGGGCGGTAt                                                >  2:778582/1‑150 (MQ=255)
                                                                                                    gaaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTcc                                             <  1:1975372/150‑1 (MQ=255)
                                                                                                    gaaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTcc                                             <  1:1558408/150‑1 (MQ=255)
                                                                                                    gaaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTcc                                             <  1:1284157/150‑1 (MQ=255)
                                                                                                    gaaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTcc                                             >  2:1750775/1‑150 (MQ=255)
                                                                                                    gaaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTcc                                             >  1:250704/1‑150 (MQ=255)
                                                                                                     aaacttaaactAGATCACCTGGTTAAAGAAGTTCTCGAATCCCTTCGGCCGGGTATCCTGGAGCTTTCCCGTACCATCTCTCACGTCGATCCGGGGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCg                                            <  2:107004/150‑1 (MQ=255)
                                                                                                      aactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGa                                           <  2:820737/150‑1 (MQ=255)
                                                                                                      aactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGa                                           >  1:794443/1‑150 (MQ=255)
                                                                                                       actgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGAc                                          <  1:1735925/150‑1 (MQ=255)
                                                                                                       actgaaactCGATCACCTGGGTAAAGAAGTTCACGAATCCCGGCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGAc                                          <  1:198767/150‑1 (MQ=255)
                                                                                                        ctgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGAcc                                         <  2:242061/150‑1 (MQ=255)
                                                                                                        ctgaaactCGATCACCTGGGAAAAGAAGTGCTCGAATCCCGGCGGCCGGGTATCCTGGAGCTTTGCCGTACCTTCGCTCACGTCGATACGGTGAAAGAGCCGTTTCCGGTTATCCCAACCTGTCACTACATGATGGGCTGTATTCCGAcc                                         <  2:1433581/150‑1 (MQ=255)
                                                                                                         tgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGCCCa                                        >  1:679833/1‑150 (MQ=255)
                                                                                                            aactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAg                                     <  2:568019/150‑1 (MQ=255)
                                                                                                            aactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAg                                     <  1:2081158/150‑1 (MQ=255)
                                                                                                              ctCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGtt                                   >  2:890403/1‑150 (MQ=255)
                                                                                                                     aCCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTc                            >  2:1868888/1‑150 (MQ=255)
                                                                                                                     aCCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTc                            >  1:749816/1‑150 (MQ=255)
                                                                                                                      ccTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCa                           <  1:1255712/150‑1 (MQ=255)
                                                                                                                      ccTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCa                           >  2:1941627/1‑150 (MQ=255)
                                                                                                                           gTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCCCGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCACCCTGTCCCTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCac                      >  2:1456552/1‑150 (MQ=255)
                                                                                                                           gTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCac                      <  1:1310468/150‑1 (MQ=255)
                                                                                                                              aagaagTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactga                   >  1:1540412/1‑150 (MQ=255)
                                                                                                                               agaagTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCAGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactgac                  <  2:291958/150‑1 (MQ=255)
                                                                                                                                 aagTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGGTCACGTCGTTCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactgactg                <  2:654345/150‑1 (MQ=255)
                                                                                                                                 aagTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTTTCCCCCCCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactgactg                >  2:336047/1‑150 (MQ=255)
                                                                                                                                  agTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGCGCCGATTCCGGTTATCCCAACCTGTAACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACtgt               >  2:2007948/1‑150 (MQ=255)
                                                                                                                                   tttCTCGAATCCCTTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTTAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACtgtg              <  2:1791056/149‑1 (MQ=255)
                                                                                                                                   gTTCTCGAATCCCGTCGGCCGGGAATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACtgtg              <  1:234025/150‑1 (MQ=255)
                                                                                                                                    ttCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGa             <  1:245706/150‑1 (MQ=255)
                                                                                                                                     tctgGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGaa            >  1:212093/1‑150 (MQ=255)
                                                                                                                                     tctcGAATCCCGTCGGCCGGGTATCCAGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCACGTTATCCCAACCTGTCACTACATGATGGGCGGTATTACGACCAAAGTTACCTCTCAGGCAATTACTGTGaa            >  2:1618948/1‑150 (MQ=255)
                                                                                                                                      ctcGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACAGGTCAGGCACTGACTGTGAAt           >  2:1752153/1‑150 (MQ=255)
                                                                                                                                       tcGACTCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGCGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATg          <  2:1182505/150‑1 (MQ=255)
                                                                                                                                       tcGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTTCCGGTCAGGCACTGACTGTGAATg          <  2:2166964/150‑1 (MQ=255)
                                                                                                                                          aaTCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATgaga       <  2:250704/150‑1 (MQ=255)
                                                                                                                                          aaTCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATgaga       <  2:770701/150‑1 (MQ=255)
                                                                                                                                              ccGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAgg   <  1:376885/150‑1 (MQ=255)
                                                                                                                                              ccGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGAGTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAgg   >  1:269608/1‑150 (MQ=255)
                                                                                                                                               cGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGc  <  2:467529/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGC  >  NC_000913/756739‑757031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: