Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,967,404 G→T V330F (GTT→TTT)  rho → transcription termination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,967,4040GT77.1% 322.0 / 104.9 180V330F (GTT→TTT) rhotranscription termination factor
Reads supporting (aligned to +/- strand):  ref base G (18/23);  new base T (66/72);  total (84/96)
Fisher's exact test for biased strand distribution p-value = 7.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01

Only 100 of 181 total aligned reads displayed.

CAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAA  >  NC_000913/3967258‑3967553
                                                                                                                                                  |                                                                                                                                                     
cAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGCCCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTa                                                                                                                                                    >  2:1386475/1‑150 (MQ=255)
   gTAAAGTGTAGAACGCTGGTGTCGAAGAAAACTACAAACATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCt                                                                                                                                                 >  2:1613364/1‑150 (MQ=255)
    tAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTa                                                                                                                                                <  1:492648/150‑1 (MQ=255)
     aaaGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGTCCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTAc                                                                                                                                               <  1:1947087/150‑1 (MQ=255)
     aaaGTGTTCACCGGTGGTGTGGATGCCACCGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAACAGGGCGGCAGCCTGACCATTAACGCGACGGCGCTTATCGATACCTGGTCTAAAATCGTCGAATTTATCTAc                                                                                                                                               >  2:300947/1‑150 (MQ=255)
        gtgtTGCCCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGCTACCGGTTCTAAAATGGACGAAGTTATCTACGaa                                                                                                                                            >  1:104029/1‑150 (MQ=255)
          gctGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAaga                                                                                                                                          >  1:1514545/3‑150 (MQ=255)
             gACCGGTGGTGTGGATGCCAAGGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGtt                                                                                                                                       <  1:1401254/150‑1 (MQ=255)
             gACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGtt                                                                                                                                       >  2:503597/1‑150 (MQ=255)
               ccGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTa                                                                                                                                     <  2:1278930/150‑1 (MQ=255)
                 ggtggtGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTaaa                                                                                                                                   >  2:1293509/1‑150 (MQ=255)
                 ggtggtGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTaaa                                                                                                                                   >  1:529695/1‑150 (MQ=255)
                  gtggtgTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAg                                                                                                                                  <  2:489097/150‑1 (MQ=255)
                   tggtgTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAgg                                                                                                                                 <  1:1630549/150‑1 (MQ=255)
                   gggtgTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAgg                                                                                                                                 <  1:252932/149‑1 (MQ=255)
                    ggtgTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGt                                                                                                                                >  2:2024290/1‑150 (MQ=255)
                     gtgtGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTa                                                                                                                               >  1:1030599/1‑150 (MQ=255)
                       gtgGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACa                                                                                                                             <  1:1570529/150‑1 (MQ=255)
                       gtgGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACa                                                                                                                             <  2:760949/150‑1 (MQ=255)
                         ggATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAgg                                                                                                                           >  2:1261534/1‑150 (MQ=255)
                             gCCAACGCCCTTCAGCGTCCGAAACGCTTCTTTGGTGGGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCTCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAAc                                                                                                                       <  1:1103890/150‑1 (MQ=255)
                               cAACGCCCTGCATCGTCCGAAACGCTTCTATGGTGCGGCGCGTAACGTGGAACAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGGTATCTACGAAGAGTTTAAAGGTTCAGGCAACAt                                                                                                                     >  1:406514/1‑150 (MQ=255)
                                aaCGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATg                                                                                                                    <  2:536274/150‑1 (MQ=255)
                                   gCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGaa                                                                                                                 >  2:620204/1‑150 (MQ=255)
                                    cccTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAAc                                                                                                                <  1:1322877/150‑1 (MQ=255)
                                        gCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGCCGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGcc                                                                                                            >  1:1427788/1‑149 (MQ=255)
                                          aTCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCAcc                                                                                                          <  1:1881801/150‑1 (MQ=255)
                                           tCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACct                                                                                                         >  1:678750/1‑150 (MQ=255)
                                               ccGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGGGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATCGACCTGCACctctct                                                                                                     >  2:209396/1‑150 (MQ=255)
                                                cGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCtctctc                                                                                                    >  1:661118/1‑150 (MQ=255)
                                                cGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCtctctc                                                                                                    >  1:209478/1‑150 (MQ=255)
                                                 gAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCg                                                                                                   >  2:1661135/1‑150 (MQ=255)
                                                  aaaCGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGa                                                                                                                  <  1:789884/134‑1 (MQ=255)
                                                  aaaCGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGt                                                                                                  >  2:1029554/1‑150 (MQ=255)
                                                  aaaCGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATCGAACTGCACCTCTCTCGt                                                                                                  >  2:67002/1‑150 (MQ=255)
                                                   aaCGCTTCTTTGGTGCGGCGCGTAACGTGGAAGATGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTa                                                                                                 <  2:1114048/150‑1 (MQ=255)
                                                   aaCGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTa                                                                                                 <  1:1661459/150‑1 (MQ=255)
                                                       cttcttTGGTGCGGCGCGTAACGTGTAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGAt                                                                                             <  1:1476536/150‑1 (MQ=255)
                                                         tcttTCGTGCGGCGCGTAACGAGGAAGAGGGCGGCAGCCTGACCTTTATCGCGACGGCGCTTATCGATACCGGTCCTAAAATGGACGAATATATCTACGACGAGTTTAAAGGAACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCg                                                                                           <  2:406330/150‑1 (MQ=255)
                                                           tttGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCt                                                                                         <  1:1866888/150‑1 (MQ=255)
                                                           tttGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCt                                                                                         <  2:224273/150‑1 (MQ=255)
                                                           tttGGTGCGGAGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCTATACCGTTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCt                                                                                         <  2:471742/150‑1 (MQ=255)
                                                            ttGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACATCTCTCGTAAGCTCGCTg                                                                                        >  2:1424566/1‑150 (MQ=255)
                                                            tgggTGCGGCGCGTAACGTGGAAGACGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACCTGCACCATGGCACTGCACCTCTCTCGTAAGATCGCTg                                                                                        <  1:1183584/148‑1 (MQ=255)
                                                                  cggcgCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAAc                                                                                  <  2:1507939/150‑1 (MQ=255)
                                                                   ggcgCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAAcg                                                                                 <  1:1613364/150‑1 (MQ=255)
                                                                     cgcgTAACGGGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGTCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAAcgcg                                                                               <  2:1060385/150‑1 (MQ=255)
                                                                        gTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCt                                                                            <  1:1721678/150‑1 (MQ=255)
                                                                         tAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCtt                                                                           >  2:852565/1‑150 (MQ=255)
                                                                           aCGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTcc                                                                         >  2:1009692/1‑150 (MQ=255)
                                                                             gTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCg                                                                       <  2:627400/150‑1 (MQ=255)
                                                                             gTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCg                                                                       >  2:1184665/1‑150 (MQ=255)
                                                                              tGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGACTTTAAAGGTACAGGCAACATCTAACTGCACCTCTCTCGTAAGATCGCTGAAAAAAGCGTCTTCACgg                                                                      >  2:645556/1‑150 (MQ=255)
                                                                                    agGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCg                                                                <  2:1438782/150‑1 (MQ=255)
                                                                                     gggCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGa                                                               <  1:1009692/150‑1 (MQ=255)
                                                                                     gggCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGa                                                               >  1:622794/1‑150 (MQ=255)
                                                                                       gcggcAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACt                                                             <  2:1535120/150‑1 (MQ=255)
                                                                                        cggcAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTa                                                            <  1:1766448/150‑1 (MQ=255)
                                                                                            aGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAAc                                                        <  1:1184253/150‑1 (MQ=255)
                                                                                              ccTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCg                                                      >  2:1787115/1‑150 (MQ=255)
                                                                                                tGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGtt                                                    <  1:1252255/150‑1 (MQ=255)
                                                                                                   ccATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTg                                                 >  1:890988/1‑150 (MQ=255)
                                                                                                   ccATTATCGCGACGGCGCTTATCGATACCGGTGCTAAAATGGACGAATTTATCTACGAAGAGTGTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAACACGCGTCTTCCCGGCTATCGACTACAACCGTTCTg                                                 <  1:1552229/150‑1 (MQ=255)
                                                                                                    cATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTgg                                                <  2:882131/150‑1 (MQ=255)
                                                                                                    cATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTgg                                                <  1:1705805/150‑1 (MQ=255)
                                                                                                    cATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTgg                                                <  2:1761253/150‑1 (MQ=255)
                                                                                                      ttATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTa                                              >  2:1478865/1‑150 (MQ=255)
                                                                                                        aTCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGGCTTCCCGGCTATCGACTACAACCGTTCTGGTAcc                                            <  1:1384421/150‑1 (MQ=255)
                                                                                                        aTCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACTGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAAACGTTCTGGTAcc                                            >  1:709316/1‑150 (MQ=255)
                                                                                                        aTCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTAcc                                            >  1:1262754/1‑150 (MQ=255)
                                                                                                          cgcgAGGGCGCTTATCTATACCGGTTCTAAAATGGACGAATTTATCTAGGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCGGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCg                                          <  1:540452/150‑1 (MQ=255)
                                                                                                          cgcgACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCg                                          <  1:734333/150‑1 (MQ=255)
                                                                                                             gACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTaa                                       >  1:758404/1‑150 (MQ=255)
                                                                                                                 gcgcTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGCACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGATATCGACTAAAACCGTTCTGGTACCCGGAaagaa                                   >  2:212631/1‑150 (MQ=255)
                                                                                                                 gcgcTTATCGATACCGGTTCTAAAATGGACGAATTTATCGACGAAGCGGTTAACGGTACAGGCAACATGGAACTGCACCGCTCTCGTAATATCGCTGAAAACCGCGTCTACCCGGCTATCGACGACACCCGTTCGGGGACCCGGAaagaa                                   >  2:219758/1‑150 (MQ=255)
                                                                                                                   gcTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAagaaga                                 <  2:758404/150‑1 (MQ=255)
                                                                                                                    cTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAagag                                >  2:1077867/1‑150 (MQ=255)
                                                                                                                    cTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAagag                                >  2:830504/1‑150 (MQ=255)
                                                                                                                         cGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAgctgct                           <  1:915833/150‑1 (MQ=255)
                                                                                                                         cGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAgctgct                           <  2:577549/150‑1 (MQ=255)
                                                                                                                            tACGGGTTCTAAAATGGACGAATTTATCTACGTAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAATACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCAc                        <  1:1157388/150‑1 (MQ=255)
                                                                                                                            tACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCAc                        <  2:1245885/150‑1 (MQ=255)
                                                                                                                            tACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCAc                        <  2:1684425/150‑1 (MQ=255)
                                                                                                                             aCCGGTTCTAAAATGCACGAATTTATCAACGAAGAGTTTAAAGGGACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAACCGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCGCACg                       <  2:564698/150‑1 (MQ=255)
                                                                                                                               gggTTCTAAATTGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGAc                     <  2:1736179/149‑1 (MQ=255)
                                                                                                                               cGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGAc                     >  2:500663/1‑150 (MQ=255)
                                                                                                                                ggTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTAAAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGGGTCTTCCCGGCTATCGACTACAACAGGTCTTGTACCCGTAATGAAGCGCTGCTACCGACt                    >  2:2145899/1‑150 (MQ=255)
                                                                                                                                 gTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCACTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAGAGAAGAGCTGATACCGACTc                   >  1:1644110/1‑150 (MQ=255)
                                                                                                                                  ttCAAAAATGCACGAATTTATCTACGAAGAGTTAAAAGGTACAGCCAACATGGAACTGCACCTCTCGCGTCAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACACCCGTTCTGGTACCCGTAAATAAGAGCTCCTCACGACTCa                  <  2:1841674/150‑1 (MQ=255)
                                                                                                                                    cTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTTAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAgg                <  2:1949942/150‑1 (MQ=255)
                                                                                                                                    cTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGCGCTGCTCACGACTCAgg                >  2:1432385/1‑150 (MQ=255)
                                                                                                                                     tAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGCACCCGTAAAGAAGAGCTGCTCACGACTCAGga               >  2:786501/1‑150 (MQ=255)
                                                                                                                                      aaaaTGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGATCTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGgaa              >  2:2002050/1‑150 (MQ=255)
                                                                                                                                      aaaaTGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGgaa              >  2:18709/1‑150 (MQ=255)
                                                                                                                                       aaaTGGACGAATTAATATACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCTTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGgaag             <  1:2007338/150‑1 (MQ=255)
                                                                                                                                           ggACGAATTAATCTACTAAGAGTTTAAAGGTACAGGCAACATGTAACTGCACCTCTCTCGTAATATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACt         <  2:2103881/150‑1 (MQ=255)
                                                                                                                                            gACGAATTTATCTACGAATAGTTTAAAGGTACAGGCAAAATGGAACTGCACCTCTCTCGTACTATCGCTGAAAAACGCGTCTTCCCGGCTATCGAATACAACCGTTCTGGTACCCTTAAAGACTAGCTTCTCACGACTCAGGAAGAACTg        <  2:1597193/150‑1 (MQ=255)
                                                                                                                                               gAATTTATCTACGAAGAGTTTAATGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAg     <  2:1880611/150‑1 (MQ=255)
                                                                                                                                               gAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAg     <  2:800756/150‑1 (MQ=255)
                                                                                                                                                  gTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGaaa  >  1:2129845/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAA  >  NC_000913/3967258‑3967553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑