Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 164,581 A→C 12.4% intergenic (+47/‑149) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913164,5810AC12.4% 115.2 / 5.8 66intergenic (+47/‑149)hrpB/mrcBputative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (22/35);  new base C (2/7);  total (24/42)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGG  >  NC_000913/164435‑164720
                                                                                                                                                  |                                                                                                                                             
tGAGGTGCAAAAAGAGATGAATGGGCGTTATCCCAAACATGTCGGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCa                                                                                                                                            <  2:2186224/150‑1 (MQ=255)
tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAACCTTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa                                                                                                                                            <  2:226201/150‑1 (MQ=255)
tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa                                                                                                                                            >  2:2572852/1‑150 (MQ=255)
tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa                                                                                                                                            <  2:226175/150‑1 (MQ=255)
tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa                                                                                                                                            >  2:1999978/1‑150 (MQ=255)
tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGGAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATcc                                                                                                                                            >  2:805439/1‑149 (MQ=255)
       cAAAAAGTGATGATTGGGCGTTATCCCAAACATGTCTGGCGGGACGACCCGGCAAATACTGCACCGACGCGAGGGACTAAAATTTTTTGGTATGGTTGGTTTTCTCCCTCTCCGTGTGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCa                                                                                                                                     <  2:1913029/150‑1 (MQ=255)
       cAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCa                                                                                                                                     >  1:225813/1‑150 (MQ=255)
            atagaTGAAAGGGCGTTATCCCAAACATGTGGGGCCGGACGACCCGGCAAATAGTGCACCGACGCGACGGACGAAAAAGTTTTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTtc                                                                                                                                <  2:1669881/148‑1 (MQ=38)
            agagATGAAAGGGCGTTATCCCAAACATGTCGGGCCGGACGACCCGGCAACTACTGCACCGACGCGACGGACGAAACAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtc                                                                                                                                <  2:631201/150‑1 (MQ=255)
                 ttaacggggGTTATCCAAAACATGTCGGGCCGGACGACCCGGCAAATACTGCACCGACGCGAGGGACGAAAAAGTTTTCGTATGGTTGGTTTTCTCCATCTCCCTGGGGTAGTGGGCGGGGTTGTGGGCATCAGCGCGCACGTtcaccct                                                                                                                           <  2:994263/145‑1 (MQ=255)
                 tGAACGGGCTTTACCCCAAACTTTTCGGGCGGGACGACCGGGAAATTACTGCACCGAGGCTCGGTAGGAAAACTTTTTGGTATGTTTGGTTTTCTCCCTCTCCGTGGGGGTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTtcaccct                                                                                                                           <  2:2015478/150‑1 (MQ=255)
                  gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtgacccac                                                                                                                          >  1:281294/1‑148 (MQ=255)
                  gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtgacccac                                                                                                                          >  1:2460339/1‑148 (MQ=255)
                  gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcacccac                                                                                                                          >  2:1681345/1‑148 (MQ=255)
                   aaaGGGCTTAAACCAAAACATGACTCCCCGCACCACCCCCAAAAAACTGCACCCACCCCACGCACTAAAAACAATACTAAAGTTTGTTTTTCCCCCACTCCCTGCGGGAGAGGGCCGGGGTGAGGCCATCAGCGCGCACGTtcaccctca                                                                                                                         <  2:734330/150‑1 (MQ=255)
                               cccAAAGTTGTGGGGCCGGACGACCGGGAAAATCCTGCCCGGCCGCGAGGGACTAAAATTTTTTCTTATGTTTGTTTTCCTCCCTCCCCGTGGGGTTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctct                                                                                                             <  2:716644/150‑1 (MQ=255)
                                ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCCCACCCCAAcacccac                                                                                                            >  1:1139713/1‑144 (MQ=37)
                                ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCACACCCCaaaaacaac                                                                                                            >  1:1336744/1‑143 (MQ=37)
                                ccAAACATGTCGGGCCGGACGCCCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTGGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                                            <  2:2289492/150‑1 (MQ=38)
                                 cAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCACACCCGAccccgcacc                                                                                                           >  1:2254989/1‑145 (MQ=38)
                                                  ccgacCCGCAAAATCCTGCACCGCCGCGACGGACTAAAACGTTTTCGTACGTTTGGTTTTCTCCCCCGCCCGGTGGGAGCGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAc                                                                                          <  2:337949/149‑1 (MQ=255)
                                                  acgacCCGGCAAATATTGCACCGACGCGACGCACGAAAAATTATTCCTAATGTTGTTTTTCTCCCTATCCGTTTGGGTGAGGGCGGGGGTTAGGGCATCAGCGCGCACGTTCACACTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAc                                                                                          <  2:337941/150‑1 (MQ=255)
                                                       ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGCTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCCCACCCAAACCCAAACCCCCCACGGAGAggggcgccctc                                                                                     >  1:1565684/1‑143 (MQ=25)
                                                        cGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAACCCACACCCACAAGGGAGAGGGGCCCGATCg                                                                                    >  2:1726721/1‑150 (MQ=38)
                                                          gCAAATCCGCCACCGCCGCGCCGGACGAAAATGTATTCGTACGGTTGGTTTCCGCCCTCCCCGGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg                                                                                  <  2:817229/150‑1 (MQ=255)
                                                                    cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGAAACCGCACACAAAACAAAAACCAAAAGGGTGTGGGGACCCATAGAGCACaaaatat                                                                       >  1:2135200/1‑146 (MQ=16)
                                                                        gacgcgacgGACGAAAATGTATTCGTAAGGTTGGTTTTCTCCCTCGCCCTGTGGGAGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgaga                                                                   <  1:1860830/150‑1 (MQ=255)
                                                                        gacgcgacgGACGAAAAAGTCTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgaga                                                                   <  1:1860826/150‑1 (MQ=255)
                                                                                 gacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttc                                                          <  1:2144853/150‑1 (MQ=255)
                                                                                      aaaaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCGAACACACAACCACAAAGGAGAGGGGACCCATCGAGCCCAAAATTTGATAGTTATCTTATTCTg                                                     >  2:1691205/1‑150 (MQ=25)
                                                                                          ttATTCGTATGGTTGGTTTTCTCCCTCGCCCGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt                                                <  1:2528591/149‑1 (MQ=255)
                                                                                          ttATTCGTATGGTTGGTTTTCGCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt                                                <  1:2524832/149‑1 (MQ=255)
                                                                                          gTATTCGTAAGGTTGGTTTTCTCCCACTCCCGGTGGGAGCGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt                                                <  2:983726/150‑1 (MQ=255)
                                                                                                gtccggttggttTTCTCCCTCGCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCCCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACa                                          <  2:1270371/146‑1 (MQ=255)
                                                                                                        ggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCACACCCCAACCCACTCCCGCAAGGGAGAGGGGCCCGATCGAGCCCAAATTTTGAGAGATATGTTCTTCTGTCGTGTAACAGAAGAACa                                  >  2:1080506/1‑150 (MQ=37)
                                                                                                        ggttTTCTCCCTCGCCCTGTGGGAGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCTAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACa                                  <  1:2351867/150‑1 (MQ=255)
                                                                                                        ggttTTCGCCCTCGCCGGGGGGGTGCGGGCCGGGGTGTGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACa                                  <  1:1414297/150‑1 (MQ=255)
                                                                                                        ggttTCCCCCCTCGCCCTGGGGGCGTGGGCCGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACa                                  <  2:2248170/150‑1 (MQ=255)
                                                                                                            ttCTCCCTCTCCGTGGGGTTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGaaa                              <  1:1869396/150‑1 (MQ=255)
                                                                                                            atctccctctcccTGGGGGTGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGaaa                              <  1:1869397/149‑1 (MQ=255)
                                                                                                             tctcccgctcccTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTTACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGaaaa                             <  1:2572852/150‑1 (MQ=255)
                                                                                                               tccctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc                           <  2:1431167/150‑1 (MQ=255)
                                                                                                               tccctctccctGTGGGAGAGGGCCGGGGGGCGCGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGAGATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc                           <  2:409111/150‑1 (MQ=255)
                                                                                                                 cctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCGAAACCACAGCCACAAGGGGGAGGGGGCCGAGCGAGCGCCAAAGTTGTGGGATATGTTGATATGTCTTTTTAGAGAACTACAGaaaaagat                         >  2:1869396/1‑146 (MQ=12)
                                                                                                                 ccgcccccgGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCgg                         <  2:1770878/145‑1 (MQ=255)
                                                                                                                  ctctccctGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGCACCCACACCCGAACCCACTACCACACCGGGCGGGGGGCCCATCGGGCCCCAAGTTTGTGAGATATCTTAGTCGGTCTTGTTACAGGAGAACAGAAAATCggg                        >  2:2483359/1‑150 (MQ=16)
                                                                                                                  ctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCACACCCCAAACCACGCCCACCAGGGGGAGGGGGCCCCTCGCGCACAAATTTTGTGTGATATCTTATTGAGTGTTGTTACAGAAGAACAGAAAAACggg                        >  2:190855/1‑150 (MQ=19)
                                                                                                                  ctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCCCACCCAAAACCTCACCCACAAGGGGGGGGGGACCCAGCGAGCCCAAAATTTTAGAGATATATTCTTCTGTATTGTAAAAGAAAAACCGaaaaaacgg                        >  2:1306858/1‑145 (MQ=19)
                                                                                                                     cccctGGGGGCGGGGGGGGGGGGGTGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTGTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCtt                     <  2:656660/149‑1 (MQ=255)
                                                                                                                       cctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCGAAACCACTCCCGCACGGGAGTGGGGGCCCATCGCGCCCAAAATTTTTGAGATATCTTATTCTGTGGTGTAACAGAAGAACACAAAAACGGGCttgt                   >  2:1052343/1‑148 (MQ=19)
                                                                                                                       cctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCAACCACACCCCAAACCACTCCCGCAAGGGCGCGGGGCCCGAAAGCGCCCCAAATTTGAGTGATATATTCTTATGTGTTGTGACAGAATGACAGAAAAACggggggtt                   >  2:1052544/1‑145 (MQ=16)
                                                                                                                         tgtgGGAGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgc                 <  2:927603/150‑1 (MQ=255)
                                                                                                                           tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc               <  1:1306858/150‑1 (MQ=255)
                                                                                                                           ggggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc               <  2:331918/149‑1 (MQ=255)
                                                                                                                            gggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCACAACCAAACCCACAACCACAAGGGTGTGGGGGCCCCTCGAGCCCAAAATTTGTGAGATATATTCTTGGGTGTTTGTAAACAAGCACAGAAAATAGGGCTTTTGCGcc              >  2:1679662/1‑150 (MQ=255)
                                                                                                                            gggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCGCACCCGAAACCACTCCCACAACGGGGAGGGGGCCGATCGGGCACCAAGTTGTTGAGATATCTTGTTAGGTCGTTTGAACGCAGAACAGGAACTCGGGGGTTTGCGcc              >  2:1679361/1‑150 (MQ=255)
                                                                                                                             ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt             <  2:315873/150‑1 (MQ=255)
                                                                                                                                 agGGCCGGGGTGAGGGCATCAGCGCGCACGTTGCCCCGCACCCCAAACCACACCCGCCAGGGCGAGGGGGCCGCTAGAGCCCACAATTTTGTATAAATCTTGATGTGTGTTTGTACAGAAGGACAGAAAATAGGGCGTTTGCGccgggat         >  2:2518087/1‑145 (MQ=255)
                                                                                                                                     ccGGGGTGAGGGCATCACCGCGCACGTTCACCCTCACCCTAACCCACTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAACTTCTACTGACTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTg     <  2:1093807/150‑1 (MQ=255)
                                                                                                                                       ggggTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg   <  2:1336744/150‑1 (MQ=255)
                                                                                                                                       ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg   <  2:1681190/150‑1 (MQ=255)
                                                                                                                                       ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg   <  2:145560/150‑1 (MQ=255)
                                                                                                                                       ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg   <  2:1072697/150‑1 (MQ=255)
                                                                                                                                       ggggCGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTCACCCTCTCCCTCAAGGGAGAGGGGACCGCTCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg   <  1:2514480/150‑1 (MQ=255)
                                                                                                                                        gggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg  <  2:1427740/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
TGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGG  >  NC_000913/164435‑164720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: