Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,767,667 T→G 6.5% intergenic (+312/‑43) rnlB → / → yfjP CP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; 50S ribosome‑binding GTPase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,767,6670TG 6.5% 478.8 / 19.5 140intergenic (+312/‑43)rnlB/yfjPCP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
Reads supporting (aligned to +/- strand):  ref base T (72/57);  new base G (7/2);  total (81/59)
Fisher's exact test for biased strand distribution p-value = 3.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

Only 100 of 142 total aligned reads displayed.

AATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGA  >  NC_000913/2767520‑2767816
                                                                                                                                                   |                                                                                                                                                     
aataatGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTcg                                                                                                                                                     >  2:2575858/1‑150 (MQ=255)
aataatGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTcg                                                                                                                                                     <  1:1409734/150‑1 (MQ=255)
  taatGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTcgcg                                                                                                                                                   <  1:870747/150‑1 (MQ=255)
      gTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTc                                                                                                                                               <  1:2253995/150‑1 (MQ=255)
      gTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTc                                                                                                                                               >  1:520159/1‑150 (MQ=255)
         aCCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCGGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCGCGAGGTTCTGc                                                                                                                                            <  2:1557465/150‑1 (MQ=255)
         aCCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGc                                                                                                                                            >  2:1979096/1‑150 (MQ=255)
         aCCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCGCGAGGTTCTGc                                                                                                                                            <  2:1557414/150‑1 (MQ=255)
          ccAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGcc                                                                                                                                           >  1:108107/1‑150 (MQ=255)
          ccAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGcc                                                                                                                                           <  2:272931/150‑1 (MQ=255)
           cAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCg                                                                                                                                          >  2:1681178/1‑150 (MQ=255)
           cAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCg                                                                                                                                          >  2:1038727/1‑150 (MQ=255)
           cAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCg                                                                                                                                          >  2:1359961/1‑150 (MQ=255)
           cAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCg                                                                                                                                          >  2:539447/1‑150 (MQ=255)
           cAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCg                                                                                                                                          >  2:723541/1‑150 (MQ=255)
           cAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGGTCTGCCg                                                                                                                                          >  1:1367205/1‑150 (MQ=255)
              ttttCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAAGCTATGTGCATGGAAAAAATCTTCAGATAGATATCATTCTCCTGAACCAGCCGATGAACGGCAGAACCTCGCGGTTCTGCCGttg                                                                                                                                       >  2:1232946/1‑149 (MQ=255)
                ttCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGttttt                                                                                                                                     <  1:1442792/150‑1 (MQ=255)
                ttCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGttttt                                                                                                                                     >  1:1058898/1‑150 (MQ=255)
                   tGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTGTGCCGTTTTTGtt                                                                                                                                  >  2:455613/1‑150 (MQ=255)
                     gAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGtttt                                                                                                                                <  1:1688267/150‑1 (MQ=255)
                     gAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGtttt                                                                                                                                >  1:753859/1‑150 (MQ=255)
                     gAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGtttt                                                                                                                                >  1:1582576/1‑150 (MQ=255)
                      aaaTCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTa                                                                                                                               <  1:2413808/150‑1 (MQ=255)
                      aaaTCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTa                                                                                                                               <  2:1372117/150‑1 (MQ=255)
                            tATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTc                                                                                                                         >  2:1968213/1‑150 (MQ=255)
                               gatgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCaga                                                                                                                      <  1:1180015/150‑1 (MQ=255)
                                    cTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAgt                                                                                                                 >  1:1342293/1‑150 (MQ=255)
                                    cTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAgt                                                                                                                 >  2:342508/1‑150 (MQ=255)
                                    cTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAgt                                                                                                                 >  1:1113233/1‑150 (MQ=255)
                                    cTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAgt                                                                                                                 >  2:1952303/1‑150 (MQ=255)
                                     tttCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAgtg                                                                                                                <  1:1386653/150‑1 (MQ=255)
                                     tttCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTGTGTTTTACTTTTCAGAGGAgtg                                                                                                                >  1:768049/1‑150 (MQ=255)
                                          cGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGtgat                                                                                                           >  2:950612/1‑150 (MQ=255)
                                            gTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATTTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTAATTTTCAGAGGAGTGGtgatga                                                                                                         >  2:1579005/1‑150 (MQ=255)
                                                 tGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAAt                                                                                                    >  1:1824889/1‑150 (MQ=255)
                                                   gTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCTGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGTTGATGAAAAAttt                                                                                                  <  2:785515/150‑1 (MQ=255)
                                                   gTGCTGTGAGTTCAGAAAGATTCGGCAATCTATTTGAATTGAAAAATTCTTCAGATAGTTATCATTCACCTGAACCATCCGCTGAACGGCATAAGCTCGCCGTTCTGCCTTTTTTTTTTTACTTTTCAGAGGATTTTTGATGAAAAAttt                                                                                                  <  2:785477/150‑1 (MQ=255)
                                                    tGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGATTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAAtttt                                                                                                 <  1:818606/150‑1 (MQ=255)
                                                      cTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGa                                                                                               <  1:91789/150‑1 (MQ=255)
                                                      cTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGa                                                                                               <  2:100819/150‑1 (MQ=255)
                                                       tgtgAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGaa                                                                                              <  2:2338070/150‑1 (MQ=255)
                                                       tgtgAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGaa                                                                                              >  2:1874805/1‑150 (MQ=255)
                                                            gTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCt                                                                                         >  1:510469/1‑150 (MQ=255)
                                                            gTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCt                                                                                         >  1:587116/1‑150 (MQ=255)
                                                            gTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCt                                                                                         <  1:2282562/150‑1 (MQ=255)
                                                             ttCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTg                                                                                        >  1:442568/1‑150 (MQ=255)
                                                             ttCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAAGCCGATGAACGGCAGAACCTCGCGGTTCTGCCGGTTTGGGTTTACTGTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTg                                                                                        >  1:2083624/1‑150 (MQ=39)
                                                              tCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATTTTCAGATAGATATCATTCTCCTGAACAATCTGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGc                                                                                       <  2:486820/150‑1 (MQ=255)
                                                               cAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCa                                                                                      <  1:2354326/150‑1 (MQ=255)
                                                                  aaaGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGcc                                                                                   >  1:265899/1‑150 (MQ=255)
                                                                       ttCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTAc                                                                              >  1:1657154/1‑150 (MQ=255)
                                                                         cTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAg                                                                            >  1:478700/1‑150 (MQ=255)
                                                                         cTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATACTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTGTTTGTGTTACTTTTAAGAGGAGTGGTGATGAAAAATTTTTAAGTTCTGCAGCCACTACAg                                                                            >  2:954937/1‑150 (MQ=39)
                                                                          tGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGa                                                                           >  1:290883/1‑150 (MQ=255)
                                                                          tGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGa                                                                           >  2:1069742/1‑150 (MQ=255)
                                                                           gCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGaa                                                                          <  2:1058898/150‑1 (MQ=255)
                                                                              aTCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAATATTTTGAAGTTATGCAGCCACTACAGAACTc                                                                       >  1:1507797/1‑150 (MQ=255)
                                                                                cTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTGTTGTTTTACTTTTCAGAGGAGGGGTGATGAAAAATTATGAAGTTCTGCAGCCACTACAGAACTccc                                                                     >  2:2362333/1‑150 (MQ=255)
                                                                                        aTTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGt                                                             <  2:753859/150‑1 (MQ=255)
                                                                                            aaaaaaTCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCCGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTAc                                                         <  2:1114722/150‑1 (MQ=255)
                                                                                              aaaaTCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCg                                                       <  1:639693/150‑1 (MQ=255)
                                                                                               aaaTCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGc                                                      <  1:2548554/150‑1 (MQ=255)
                                                                                                    ttCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATgg                                                 <  1:1650072/150‑1 (MQ=255)
                                                                                                    ttCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATgg                                                 <  1:945421/150‑1 (MQ=255)
                                                                                                    ttCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATgg                                                 <  1:443283/150‑1 (MQ=255)
                                                                                                       agatagatATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGGGTGGTGATGAAAAATTTTTAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCGATGGGTa                                              >  1:908773/1‑150 (MQ=255)
                                                                                                         atagataTCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATc                                            >  2:2048680/1‑150 (MQ=255)
                                                                                                           agataTCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTGTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACGACAGAACACCCATTCCGGTCTACCGCGATGGGGATCTg                                          >  1:1937317/1‑150 (MQ=255)
                                                                                                             atatCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGaa                                        <  1:316130/150‑1 (MQ=255)
                                                                                                             atatCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGaa                                        <  2:1342293/150‑1 (MQ=255)
                                                                                                              tatCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAAc                                       <  1:2204403/150‑1 (MQ=255)
                                                                                                              tatCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAAc                                       <  2:2553667/150‑1 (MQ=255)
                                                                                                              tatCAGTCTCCTGAACAATCCGCTGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAAc                                       <  1:2244450/150‑1 (MQ=255)
                                                                                                                tCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGGGTGGTGATGAAGAAGTTTGAAGTTCTGCAGCCACTACAGTACTCCCTTTCCGGTCTACCGCGCTGGGTATCTGAACgg                                     >  2:1447478/1‑149 (MQ=255)
                                                                                                                 cATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGta                                    <  1:1918076/150‑1 (MQ=255)
                                                                                                                   ttCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGtata                                  >  2:1503944/1‑150 (MQ=255)
                                                                                                                   ttCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGtata                                  <  2:2165676/150‑1 (MQ=255)
                                                                                                                   ttCTCCTGAACAATCCGATGAACGGCAGAACCACGCGGTTCTGCCGTTTTTGTTTTACTTTTGAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACAACAGAACGCCCATTCCGGTCTAACGCAATGGGTATCTGAACGtata                                  >  1:719126/1‑150 (MQ=255)
                                                                                                                        cTGAACACTCCGATGACCGGCAGAACCTCGCGGTTCTGCCGATTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgca                             <  2:2040759/150‑1 (MQ=255)
                                                                                                                        cTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgca                             <  1:1505126/150‑1 (MQ=255)
                                                                                                                        cTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgca                             <  2:854707/150‑1 (MQ=255)
                                                                                                                        cTGAACAATCCGAAGAACGGCAGAACCTCGCGGTACTGCCGAATTTGTTTTACTTTACAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgca                             <  2:69378/150‑1 (MQ=255)
                                                                                                                         tGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgcag                            >  2:2266247/1‑150 (MQ=255)
                                                                                                                         tGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgcag                            >  2:1978985/1‑150 (MQ=255)
                                                                                                                                tCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGCAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTCTCTGAACGTCTCTTGCAGCAGAGaa                     >  2:2524077/1‑150 (MQ=255)
                                                                                                                                tCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATaa                     >  2:1961685/1‑150 (MQ=255)
                                                                                                                                tCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATaa                     >  2:1125788/1‑150 (MQ=255)
                                                                                                                                tCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATaa                     >  2:1164702/1‑150 (MQ=255)
                                                                                                                                tCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATaa                     >  2:862109/1‑150 (MQ=255)
                                                                                                                                   gATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATc                  >  2:2246673/1‑150 (MQ=255)
                                                                                                                                   gATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATc                  >  2:398816/1‑150 (MQ=255)
                                                                                                                                        aCGGCAGAACCGCGAGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTa             >  2:117349/1‑150 (MQ=255)
                                                                                                                                         cGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTaa            <  1:1210149/150‑1 (MQ=255)
                                                                                                                                         cGGCAGAACCGCGAGGTTCTGCCGTTTGTGTTTTACTTTTCAGAGGAGGGGTGATGCAAAATTTTGAAGTTCTGCAGCCACTCCAGAACTCCCTGTCCGGTCTACCGCTATGGGTATCGGAACGTCTCTTGCAGCAGATAACTCAGttca            >  2:1344044/1‑148 (MQ=255)
                                                                                                                                            cAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATGTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTAACGCTAGCGGTATCTGAACGAATATTGCAGCAGATACATCAGTTAAccc         >  2:1332490/1‑150 (MQ=255)
                                                                                                                                            cAGAACCGCGAGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAAccc         >  2:826881/1‑150 (MQ=255)
                                                                                                                                            cAGAACCGCGAGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAAccc         >  2:689335/1‑150 (MQ=255)
                                                                                                                                             aGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCa        <  1:342508/150‑1 (MQ=255)
                                                                                                                                                   tCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGa  <  2:265899/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGA  >  NC_000913/2767520‑2767816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: