BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,817,916	T→G	11.3%	intergenic (‑56/+143)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,916	0	T	G	11.3%	284.2 / 32.8	107	intergenic (‑56/+143)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (44/51); new base G (6/6); total (50/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.65e-01

Only 100 of 111 total aligned reads displayed.

GAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATG  >  NC_000913/2817767‑2818060

gAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAt                                                                                                                                                  <  1:2132438/150‑1 (MQ=255)

gAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAt                                                                                                                                                  >  2:473396/1‑150 (MQ=255)

gAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTAATTCTACTGATAATATTGAACCCCATACTAAACACATATCAAt                                                                                                                                                  >  2:1143429/1‑150 (MQ=14)

 aaGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAtt                                                                                                                                                 <  2:1695808/150‑1 (MQ=255)

   ggTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTaa                                                                                                                                               <  1:1860056/150‑1 (MQ=255)

   ggTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTaa                                                                                                                                               <  2:2382008/150‑1 (MQ=255)

   ggTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTaa                                                                                                                                               <  2:747697/150‑1 (MQ=255)

   ggTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGGAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTaa                                                                                                                                               <  2:908012/150‑1 (MQ=255)

    gTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAag                                                                                                                                              >  2:1963302/1‑150 (MQ=255)

           agaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGT                                                                                                                                       <  2:750959‑M1/150‑1 (MQ=255)

                   ntgCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGG                                                                                                                               >  1:1203363‑M1/2‑150 (MQ=255)

                   gTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGG                                                                                                                               <  1:653114‑M1/150‑1 (MQ=255)

                   gTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGG                                                                                                                               >  1:950274‑M1/1‑150 (MQ=255)

                   gTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGG                                                                                                                               >  1:1203367‑M1/1‑150 (MQ=255)

                        tCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGA                                                                                                                          <  1:347928‑M1/150‑1 (MQ=255)

                              aggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTc                                                                                                                    <  1:763704/150‑1 (MQ=255)

                                  ttCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAAATTATTCTACTGCTGATATTGATACCGCTAGTAAAGCCATATCAATTAAGTGATGGTGCAGCCGGGAGGATTccacgccg                                                                                                                >  1:565525‑M1/1‑142 (MQ=255)

                                      aaCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTcgaaccgacgac                                                                                                            >  1:1302901‑M1/1‑138 (MQ=255)

                                          tCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTc                                                                                                        <  1:1912522/150‑1 (MQ=255)

                                                   tCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCgg                                                                                               >  1:901350/1‑150 (MQ=255)

                                                   tCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTAGCATGGCGGCTCCCCCGTCgg                                                                                               >  1:1271039/1‑150 (MQ=17)

                                                       ttCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCg                                                                                           <  1:648562/150‑1 (MQ=255)

                                                        tCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGt                                                                                          <  1:198526/150‑1 (MQ=255)

                                                        tCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGt                                                                                          <  1:198536/150‑1 (MQ=255)

                                                        tCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGt                                                                                          <  1:1640553/150‑1 (MQ=255)

                                                           tAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGc                                                                                       >  2:737596/1‑150 (MQ=255)

                                                           tAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGc                                                                                       <  1:2110933/150‑1 (MQ=255)

                                                               ctaGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAATACTTATTCTACTGCTGATATTGATACAGCTACTAAAGCCATATCAATTTAGAGATGGTGCATCCGGGAGGATTACTCTCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaaa                                                                                   <  2:165088/148‑1 (MQ=255)

                                                               cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaaa                                                                                   <  1:2282594/150‑1 (MQ=255)

                                                               cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaaa                                                                                   <  1:47553/150‑1 (MQ=255)

                                                               cGAGTACTCTATCCAGCTGAGCTACGGAAGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCttta                                                                                   <  2:1955617/150‑4 (MQ=255)

                                                                  gTACTCTTTCCTGCTGCGATACGTATGCATCGGGAAAAGTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGAGGGTGCTTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCa                                                                                <  2:351810/150‑1 (MQ=17)

                                                                    aCTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAAc                                                                              <  2:114616/150‑1 (MQ=255)

                                                                     ctctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCACTTAAGAGATGGTGAAGCCGGGAGCAAAACTCGCAATGCGGATCGCCCGTCGGGCCGTTGCTAAAGccccg                                                                             >  1:2448348/1‑146 (MQ=17)

                                                                     ctctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCACCg                                                                             >  1:2013626/1‑150 (MQ=255)

                                                                     ctctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACg                                                                             >  1:694995/1‑150 (MQ=255)

                                                                     ctctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGAATCCAGGAGGAAAACTCGCATTGCGGCTCGCCCTTCGGGCCGTGGCTAACGCAACg                                                                             >  1:174035/1‑150 (MQ=17)

                                                                     ctctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGATACTAAAGCCATAACAATTAAGAGATGGTGCATCCGGGAGGATAACTCGCCGTGCGGCTCGCCCTTCGGGCCGATGCTAAAGCCACg                                                                             >  1:2448353/1‑150 (MQ=17)

                                                                     ctctATCCAGCTGAGCTACGGATGCATCGGGAAAACTAATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATAACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACg                                                                             >  1:2013623/1‑150 (MQ=255)

                                                                     ctctATCCAGCTGAGCTACGGATGCATCGGGAAAACTAATTCTACGCCGGATATTGATACCGCTACTAAAGCCATAGCACTAAAGAGAGGGTGCATCCGGCAGCAAAACACGCCATGCGGCGCCCCCTTCGGGCCGGTGCTCACGCAACg                                                                             >  1:2013513/1‑150 (MQ=17)

                                                                       ctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGGGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGtt                                                                           <  2:2110022/150‑1 (MQ=255)

                                                                       atATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGtt                                                                           <  2:513294/149‑1 (MQ=255)

                                                                          tCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatc                                                                        <  1:468462/150‑1 (MQ=255)

                                                                            ctgCTGAGATTCGTATTCATCTGTACTACTTATTCTTCTTCTTATATTGATACCGCTTATAAAGCCATATCTTTTAAGAGATGGTGCATCCGGGAGGATTACTCCCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatcat                                                                      <  1:1963581/148‑1 (MQ=14)

                                                                            cAGCTGAGCTACGGATGCTTCGGGAAAACTTATTCTACTGCTGATTTTGATACCTCTACTAAAGCCATATCAATTTAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatcat                                                                      <  1:1718763/150‑1 (MQ=255)

                                                                            cAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatcat                                                                      <  1:1718739/150‑1 (MQ=255)

                                                                              gCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATcc                                                                    >  2:2021512/1‑150 (MQ=255)

                                                                               cTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTATAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATccc                                                                   <  2:1517001/150‑1 (MQ=255)

                                                                                    cTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctctc                                                                                >  1:2376264‑M1/1‑92 (MQ=255)

                                                                                    cTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatc                                                                                <  2:2376264‑M1/132‑41 (MQ=255)

                                                                                        ggATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTg                                                          <  1:1963302/150‑1 (MQ=255)

                                                                                                cGGTAAATCTTATTCTAGTGCTGATTTTGCTAGCGATACTAAAGCCATATCAATTAAGAGATGGTGCCTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAAt                                                  <  2:1704253/150‑1 (MQ=17)

                                                                                                cGGGAATAGTTATTCTACTGCTGTTATTGATAGAGCTACTAAAGCCATATCTTTTATTAGATGGTGCCTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAAt                                                  <  2:141374/150‑1 (MQ=17)

                                                                                                 gggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCAGAGAGCCTAGCCCTACTGGCCGTTTCTAAAGCACCGATATCATCCCTAGTGCCAGCGGTTAAta                                                 >  2:1472447/1‑149 (MQ=18)

                                                                                                 gggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACACGCGTAGCGGCTCGCCCTACTAGCCGAGTCGAAAGAACCGATATCCTCCGTAGTCCCTGCGATTGAta                                                 >  2:1472460/1‑149 (MQ=17)

                                                                                                  gggaaaTTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGc                                                                                       <  1:1135757/108‑1 (MQ=11)

                                                                                                  gggaaaTTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGc                                                                                       >  2:1135757/4‑111 (MQ=11)

                                                                                                  ggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTAGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTc                                                >  2:2412956/1‑150 (MQ=255)

                                                                                                  ggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCAAGAGCCACGCCCTACTGGCCGTTTCAAAAGAAACGATATCATCCCTAGTCACTGCGGAAAATTc                                                >  2:280720/1‑150 (MQ=18)

                                                                                                      aatttattTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggg                                            >  2:915941‑M1/4‑74 (MQ=255)

                                                                                                         ttATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAcc                                         >  1:2547131/1‑150 (MQ=255)

                                                                                                         ttATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAcc                                         >  2:1951598/1‑150 (MQ=255)

                                                                                                         ttATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCAGGGCGGCTCGCCCGTCGGGCCGTTGCTCATGCAACGGTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAcc                                         >  2:2466725/1‑150 (MQ=18)

                                                                                                           aTTCTACTGCTGATATTGAAACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCATAGCGGCTCGCCCTACGTGCCGTTGCTAAAGCAACGATATCATCCCTAGTGCCTGCTGTTAATTCATCTACctc                                       >  1:1053100/1‑150 (MQ=17)

                                                                                                               tACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCGTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCg                                   >  2:2198718/1‑150 (MQ=255)

                                                                                                                   gctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATAc                               >  2:463314/1‑150 (MQ=255)

                                                                                                                   gctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATAc                               >  2:1099806/1‑150 (MQ=255)

                                                                                                                   gctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATAc                               >  2:2342693/1‑150 (MQ=255)

                                                                                                                   gctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGATATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATAc                               >  2:79024/1‑150 (MQ=255)

                                                                                                                    cttatatCGATACCGCTACTACAGCCATATCAATTCCGAGATGGTGCCTCCGGGAGGATTTCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATAcc                              <  1:846334/147‑1 (MQ=17)

                                                                                                                    ctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATAcc                              <  1:2326254/150‑1 (MQ=255)

                                                                                                                       atatTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaac                                   >  1:593849‑M1/1‑57 (MQ=255)

                                                                                                                       atatTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaac                                   >  1:593813‑M1/1‑57 (MQ=255)

                                                                                                                       atatTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaac                                   <  2:593849‑M1/142‑86 (MQ=255)

                                                                                                                       atatTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaac                                   <  2:593813‑M1/142‑86 (MQ=255)

                                                                                                                         atTGCTCCCGCTACTACAGCCATATCTATTACGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCAt                         <  1:1262708/150‑1 (MQ=17)

                                                                                                                         atTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCGGTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGATCTCATCCCTAGTGCCTGCGGTTAATTCCGCTAACTCTTCGATACCTTCAt                         >  2:1915569/1‑150 (MQ=17)

                                                                                                                         atTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCGTGCGGCTACCCCTTCGGGCCGTTGCTAAAGCAACGGTCTCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCag                         >  2:1197030/1‑149 (MQ=17)

                                                                                                                         atTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACAACCATAGCCCCGCCCCCTTCGGGCCGTTGCAAAAGAAACGTTCTCATCCCGAGTGCCAGCGGTTATTACCGCTAGCACTACGATACCTTCag                         >  2:1915502/1‑149 (MQ=9)

                                                                                                                              tACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTg                    <  2:1203367/150‑1 (MQ=255)

                                                                                                                              tACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTg                    <  2:1203363/150‑1 (MQ=255)

                                                                                                                              tACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcgg                                                     >  1:1647720‑M1/1‑50 (MQ=255)

                                                                                                                               aCCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATGCGTGAGGTTTACTCGCCTTGGGGCTCGGACTTGGGGCCTTTTCTTAAGCAACGTTATCATCCCTTGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGa                   <  1:91650/150‑1 (MQ=25)

                                                                                                                                ccGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGc                                                                                                             <  1:1564242/59‑1 (MQ=255)

                                                                                                                                ccGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGc                                                                                                             >  2:1564242/1‑59 (MQ=255)

                                                                                                                                ccGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGaa                  >  2:2487299/1‑150 (MQ=255)

                                                                                                                                ccGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGaa                  >  1:1231981/1‑150 (MQ=255)

                                                                                                                                ccGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGaa                  <  2:164794/150‑1 (MQ=255)

                                                                                                                                 cGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAg                 >  1:811619/1‑150 (MQ=255)

                                                                                                                                 cGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAg                 >  1:171045/1‑150 (MQ=255)

                                                                                                                                 cGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAg                 >  1:579508/1‑150 (MQ=255)

                                                                                                                                  gCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGa                >  2:2161212/1‑150 (MQ=255)

                                                                                                                                  gCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATT                                                                                                                        <  1:1777287‑M1/46‑1 (MQ=255)

                                                                                                                                  gCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATT                                                                                                                        >  2:1777287‑M1/1‑46 (MQ=255)

                                                                                                                                  gCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaaccccggatgcagcttttgac                >  1:1470753‑M1/1‑46 (MQ=255)

                                                                                                                                     actaAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaaccccggatgcagcttttgaccgc             <  2:1470753‑M1/150‑108 (MQ=255)

                                                                                                                                             cATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAat     <  2:901350/150‑1 (MQ=255)

                                                                                                                                             cATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaaccccggatgcagcttttgaccgcatactccc     <  1:915941‑M1/150‑116 (MQ=255)

                                                                                                                                                atCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATg  <  2:2161415/150‑1 (MQ=255)

                                                                                                                                                atCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTGGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTCGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATg  <  2:383397/150‑1 (MQ=255)

GAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATG  >  NC_000913/2817767‑2818060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`