Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,856,832 T→A 5.1% intergenic (‑26/‑261) ygbA ← / → mutS uncharacterized protein/methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,856,8320TA 5.1% 430.6 / 8.7 118intergenic (‑26/‑261)ygbA/mutSuncharacterized protein/methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base T (67/45);  new base A (4/2);  total (71/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 121 total aligned reads displayed.

GCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGT  >  NC_000913/2856683‑2856980
                                                                                                                                                     |                                                                                                                                                    
gcgcGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTAt                                                                                                                                                      <  2:895000/150‑1 (MQ=255)
gcgcGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTAt                                                                                                                                                      <  2:894996/150‑1 (MQ=255)
  gcgTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTAttt                                                                                                                                                    <  1:2010137/150‑1 (MQ=255)
   cgTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTta                                                                                                                                                   >  1:1876821/1‑150 (MQ=255)
       aacaaacaACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATAtt                                                                                                                                               >  2:1274176/1‑150 (MQ=255)
       aacaaacaACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATAtt                                                                                                                                               >  2:1274189/1‑150 (MQ=255)
        acaaacaaCGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTa                                                                                                                                              <  1:1379125/150‑1 (MQ=255)
          aaacaaCGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACa                                                                                                                                            >  2:1356489/1‑150 (MQ=255)
          aaacaaCGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACa                                                                                                                                            >  1:832232/1‑150 (MQ=255)
          aaacaaCGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACa                                                                                                                                            <  2:55936/150‑1 (MQ=255)
             caacGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatct                                                                                                                                         <  2:1696741/150‑1 (MQ=255)
               acGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatctta                                                                                                                                       >  1:1551397/1‑150 (MQ=255)
                cGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaa                                                                                                                                      <  1:1861335/150‑1 (MQ=255)
                 gCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTAATATAAATACatcttaat                                                                                                                                     <  1:952911/150‑1 (MQ=255)
                 gCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTAATATAAATACatcttaat                                                                                                                                     <  1:418030/150‑1 (MQ=255)
                  ccTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatc                                                                                                                                    >  2:1352593/1‑150 (MQ=255)
                  ccTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatc                                                                                                                                    >  2:1200358/1‑150 (MQ=255)
                    tCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCCTTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctt                                                                                                                                  >  1:1682600/1‑150 (MQ=255)
                    tCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctt                                                                                                                                  >  2:2223641/1‑150 (MQ=255)
                    tCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctt                                                                                                                                  >  2:1804641/1‑150 (MQ=255)
                    tCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctt                                                                                                                                  >  1:369998/1‑150 (MQ=255)
                    tCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctt                                                                                                                                  >  1:2317373/1‑150 (MQ=255)
                     cGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctta                                                                                                                                 >  1:851954/1‑150 (MQ=255)
                     cGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctta                                                                                                                                 >  1:1912695/1‑150 (MQ=255)
                      gTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACAtcttaatcttaa                                                                                                                                <  1:1219402/150‑1 (MQ=255)
                       tAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTaaa                                                                                                                               >  1:222891/1‑150 (MQ=255)
                          tGCTCCGGCTCCGCTGGCGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAggg                                                                                                                            <  1:671297/150‑1 (MQ=255)
                              ccggctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACt                                                                                                                        >  2:1232048/1‑150 (MQ=255)
                               cggctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTa                                                                                                                       >  2:595146/1‑150 (MQ=255)
                                ggctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTAt                                                                                                                      <  2:1531569/150‑1 (MQ=255)
                                  ctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGa                                                                                                                    <  1:2537063/150‑1 (MQ=255)
                                  ctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGa                                                                                                                    <  1:2537084/150‑1 (MQ=255)
                                  ctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGa                                                                                                                    <  1:589649/150‑1 (MQ=255)
                                  ctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGa                                                                                                                    <  1:589560/150‑1 (MQ=255)
                                   tccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACACTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGAc                                                                                                                   <  2:32533/150‑1 (MQ=255)
                                   tccgCGGACGCCAGCGGGCATTGCGCTTGATCCCGATCGATCATTTTTTGAATCGGCAGAATTTCACGAGAGATACGCTGGCCGGACATGCTGCCTCCACCTCATTAAGATCTCTATATATTACATCTTAATCTTAAAGGGCACTAAGAc                                                                                                                   >  2:2571786/1‑150 (MQ=255)
                                     cgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTc                                                                                                                 >  1:70605/1‑150 (MQ=255)
                                        tGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCaa                                                                                                              <  1:457799/150‑1 (MQ=255)
                                         gACGCCTGCGGGCTTTTCGCTTGATATAGATCGATCATTTTTTTAATCGTCTGTTTTTCAGGAGATATTCGCGTGCCGGACATGCTGCCTCGACCTCAGTTAGTGGTATTTATTTTACATCTTAATCTTAAAGGGCACTATGACTCCaaa                                                                                                             <  1:582644/150‑1 (MQ=255)
                                         gACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCaaa                                                                                                             <  1:2436138/150‑1 (MQ=255)
                                            gCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAaagaa                                                                                                          <  2:494873/150‑1 (MQ=255)
                                             ccTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAaagaag                                                                                                         >  2:1021343/1‑150 (MQ=255)
                                                gCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAaagaagaag                                                                                                      <  2:1813377/150‑1 (MQ=255)
                                                 cGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAgg                                                                                                     <  1:1908297/150‑1 (MQ=255)
                                                    gCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGtt                                                                                                  >  2:1333639/1‑150 (MQ=255)
                                                     cATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTa                                                                                                 <  2:1055615/150‑1 (MQ=255)
                                                           gCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAAc                                                                                           >  1:890730/1‑150 (MQ=255)
                                                            cTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAAcc                                                                                          >  1:128905/1‑150 (MQ=255)
                                                            cTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAAcc                                                                                          >  1:1897046/1‑150 (MQ=255)
                                                             ttGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCCCGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCg                                                                                         <  1:192648/150‑1 (MQ=255)
                                                                 tAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATAc                                                                                     <  1:1727155/150‑1 (MQ=255)
                                                                 tAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATAc                                                                                     >  2:424875/1‑150 (MQ=255)
                                                                 tAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTACACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATAc                                                                                     <  1:1009706/150‑1 (MQ=255)
                                                                         gatcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGc                                                                             >  2:1188472/1‑150 (MQ=255)
                                                                         gatcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGc                                                                             >  2:731030/1‑150 (MQ=255)
                                                                          atcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCg                                                                            <  2:300329/150‑1 (MQ=255)
                                                                          atcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCg                                                                            >  1:652525/1‑150 (MQ=255)
                                                                          atcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCg                                                                            <  2:1686423/150‑1 (MQ=255)
                                                                             aTTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTAc                                                                         >  1:681506/1‑150 (MQ=255)
                                                                              tttttttAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACt                                                                        >  2:1048027/1‑150 (MQ=255)
                                                                               ttttttAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACtt                                                                       <  1:2219492/150‑1 (MQ=255)
                                                                                 ttttAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGc                                                                     <  2:719398/150‑1 (MQ=255)
                                                                                          tCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGc                                                            >  1:1260329/1‑150 (MQ=255)
                                                                                           cAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCa                                                           >  1:1248833/1‑150 (MQ=255)
                                                                                                      gagagaTACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAg                                                >  1:2295349/1‑150 (MQ=255)
                                                                                                      gagagaTACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAg                                                >  2:1815653/1‑150 (MQ=255)
                                                                                                        gagaTACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCt                                              <  1:1232048/150‑1 (MQ=255)
                                                                                                         agaTACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTg                                             <  2:867432/150‑1 (MQ=255)
                                                                                                             aCGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCaaa                                         <  2:2307320/150‑1 (MQ=255)
                                                                                                              cGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCaaaa                                        <  2:1513838/150‑1 (MQ=255)
                                                                                                                cgtGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACa                                      <  1:2197881/148‑1 (MQ=255)
                                                                                                                 ttGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAg                                     >  1:693992/1‑150 (MQ=255)
                                                                                                                    ccGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCAt                                  <  2:2290101/150‑1 (MQ=255)
                                                                                                                     cGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATc                                 >  2:2502412/1‑150 (MQ=255)
                                                                                                                     cGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCGTCATAAGCATCACGCAAAAGCTGCAAAACAGCATc                                 >  2:231186/1‑150 (MQ=255)
                                                                                                                          aTGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTcc                            >  2:1475571/1‑150 (MQ=255)
                                                                                                                           tgctgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTccc                           <  2:1309388/150‑1 (MQ=255)
                                                                                                                           tgctgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTccc                           <  1:2403093/150‑1 (MQ=255)
                                                                                                                             ctgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCgg                         >  1:880158/1‑150 (MQ=255)
                                                                                                                             ctgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCgg                         >  1:1435961/1‑150 (MQ=255)
                                                                                                                              tgcCTCCACCTCATTAAGTTGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCATAGAAGTAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGa                        >  2:40111/1‑150 (MQ=255)
                                                                                                                               gcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGaa                       <  2:47042/150‑1 (MQ=255)
                                                                                                                                  tCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCa                    >  2:2419167/1‑150 (MQ=255)
                                                                                                                                   ctttaagaTTAAGATGTAATATAAATACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAg                   >  1:1035683/8‑150 (MQ=39)
                                                                                                                                   ctttaagaTTAAGATGTAATATAAATACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAg                   >  2:143483/8‑150 (MQ=39)
                                                                                                                                   ctttaagaTTAAGATGTAATATAAATACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGAGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAg                   >  2:883182/8‑150 (MQ=38)
                                                                                                                                   ccACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAg                   >  2:1645972/1‑150 (MQ=255)
                                                                                                                                   ccACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAg                   >  2:49995/1‑150 (MQ=255)
                                                                                                                                   acACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAATGGCACTATGACTCCAAAGAAGAAGGGTTATCCAACCGATACAAATTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCAGGAACCAg                   >  2:826580/2‑150 (MQ=255)
                                                                                                                                    tttaagaTTAAGATGTAATATAAATACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGc                  >  1:725721/7‑150 (MQ=255)
                                                                                                                                    cACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGc                  <  1:114958/150‑1 (MQ=255)
                                                                                                                                      ccTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCAt                <  2:760881/150‑1 (MQ=255)
                                                                                                                                      ccTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCAt                >  1:492755/1‑150 (MQ=255)
                                                                                                                                         cATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCaa             <  2:1551397/150‑1 (MQ=255)
                                                                                                                                             aaGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAAc         <  1:424875/150‑1 (MQ=255)
                                                                                                                                              aGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACt        >  2:208921/1‑150 (MQ=255)
                                                                                                                                              aGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACt        >  2:74880/1‑150 (MQ=255)
                                                                                                                                                aTGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCg      >  2:1626891/1‑150 (MQ=255)
                                                                                                                                                    aTTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGt  >  2:2199333/1‑150 (MQ=255)
                                                                                                                                                    aTTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGt  >  2:2199216/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGT  >  NC_000913/2856683‑2856980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: