Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,856,834 T→A 5.1% intergenic (‑28/‑259) ygbA ← / → mutS uncharacterized protein/methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,856,8340TA 5.1% 425.8 / 9.2 117intergenic (‑28/‑259)ygbA/mutSuncharacterized protein/methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base T (66/45);  new base A (4/2);  total (70/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 119 total aligned reads displayed.

GCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGT  >  NC_000913/2856685‑2856980
                                                                                                                                                     |                                                                                                                                                  
gcgTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTAttt                                                                                                                                                    <  1:2010137/150‑1 (MQ=255)
 cgTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTta                                                                                                                                                   >  1:1876821/1‑150 (MQ=255)
     aacaaacaACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATAtt                                                                                                                                               >  2:1274176/1‑150 (MQ=255)
     aacaaacaACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATAtt                                                                                                                                               >  2:1274189/1‑150 (MQ=255)
      acaaacaaCGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTa                                                                                                                                              <  1:1379125/150‑1 (MQ=255)
        aaacaaCGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACa                                                                                                                                            <  2:55936/150‑1 (MQ=255)
        aaacaaCGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACa                                                                                                                                            >  2:1356489/1‑150 (MQ=255)
        aaacaaCGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACa                                                                                                                                            >  1:832232/1‑150 (MQ=255)
           caacGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatct                                                                                                                                         <  2:1696741/150‑1 (MQ=255)
              cGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaa                                                                                                                                      <  1:1861335/150‑1 (MQ=255)
              cGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaa                                                                                                                                      <  2:2543613/150‑1 (MQ=255)
               gCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTAATATAAATACatcttaat                                                                                                                                     <  1:418030/150‑1 (MQ=255)
                ccTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatc                                                                                                                                    >  2:1352593/1‑150 (MQ=255)
                  tCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCCTTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctt                                                                                                                                  >  1:1682600/1‑150 (MQ=255)
                  tCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctt                                                                                                                                  >  2:2223641/1‑150 (MQ=255)
                  tCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctt                                                                                                                                  >  1:369998/1‑150 (MQ=255)
                  tCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctt                                                                                                                                  >  1:2317373/1‑150 (MQ=255)
                  tCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctt                                                                                                                                  >  2:1804641/1‑150 (MQ=255)
                   cGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctta                                                                                                                                 >  1:1912695/1‑150 (MQ=255)
                   cGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctta                                                                                                                                 >  1:851954/1‑150 (MQ=255)
                    gTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACAtcttaatcttaa                                                                                                                                <  1:1219402/150‑1 (MQ=255)
                     tAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTaaa                                                                                                                               >  1:222891/1‑150 (MQ=255)
                     tAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCGTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTaaa                                                                                                                               >  1:2170507/1‑150 (MQ=255)
                        tGCTCCGGCTCCGCTGGCGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAggg                                                                                                                            <  1:671297/150‑1 (MQ=255)
                          ctccggctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCa                                                                                                                          >  2:464257/1‑150 (MQ=255)
                          ctccggctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCa                                                                                                                          >  1:777188/1‑150 (MQ=255)
                            ccggctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACt                                                                                                                        >  2:1232048/1‑150 (MQ=255)
                             cggctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTa                                                                                                                       >  2:595146/1‑150 (MQ=255)
                             cggatacgCAGACGCATGCCGGAATTTCTCTTGATAAAGATCGATCATTTTATTAATCTTAAGTTTTTAACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAATATTTATTTATATTACATCTTAATCTTAAATGGCACTa                                                                                                                       >  2:847152/7‑150 (MQ=255)
                              ggctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTAt                                                                                                                      <  2:1531569/150‑1 (MQ=255)
                                ctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGa                                                                                                                    <  1:2537063/150‑1 (MQ=255)
                                ctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGa                                                                                                                    <  1:2537084/150‑1 (MQ=255)
                                ctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGa                                                                                                                    <  1:589560/150‑1 (MQ=255)
                                 tccgCGGACGCCAGCGGGCATTGCGCTTGATCCCGATCGATCATTTTTTGAATCGGCAGAATTTCACGAGAGATACGCTGGCCGGACATGCTGCCTCCACCTCATTAAGATCTCTATATATTACATCTTAATCTTAAAGGGCACTAAGAc                                                                                                                   >  2:2571786/1‑150 (MQ=255)
                                   cgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTc                                                                                                                 >  1:70605/1‑150 (MQ=255)
                                      tGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCaa                                                                                                              <  1:457799/150‑1 (MQ=255)
                                       gACGCCTGCGGGCTTTTCGCTTGATATAGATCGATCATTTTTTTAATCGTCTGTTTTTCAGGAGATATTCGCGTGCCGGACATGCTGCCTCGACCTCAGTTAGTGGTATTTATTTTACATCTTAATCTTAAAGGGCACTATGACTCCaaa                                                                                                             <  1:582644/150‑1 (MQ=255)
                                          gCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAaagaa                                                                                                          <  2:1224507/150‑1 (MQ=255)
                                          gCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAaagaa                                                                                                          <  2:494873/150‑1 (MQ=255)
                                           ccTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAaagaag                                                                                                         >  2:1021343/1‑150 (MQ=255)
                                              gCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAaagaagaag                                                                                                      <  2:1813377/150‑1 (MQ=255)
                                               cGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAgg                                                                                                     <  1:1908297/150‑1 (MQ=255)
                                                   cATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTa                                                                                                 <  2:1055615/150‑1 (MQ=255)
                                                    aTTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAg                                                                                                <  1:2310065/150‑1 (MQ=255)
                                                       tCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCa                                                                                             >  2:1346181/1‑150 (MQ=255)
                                                         gCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAAc                                                                                           >  1:890730/1‑150 (MQ=255)
                                                          cTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAAcc                                                                                          >  1:1897041/1‑150 (MQ=255)
                                                          cTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAAcc                                                                                          >  1:128905/1‑150 (MQ=255)
                                                           ttGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCCCGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCg                                                                                         <  1:192648/150‑1 (MQ=255)
                                                               tAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATAc                                                                                     <  2:1482073/150‑1 (MQ=255)
                                                               tAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATAc                                                                                     <  1:1727155/150‑1 (MQ=255)
                                                               tAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATAc                                                                                     >  2:424875/1‑150 (MQ=255)
                                                               tAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTACACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATAc                                                                                     <  1:1009706/150‑1 (MQ=255)
                                                                   gatcgatcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAAtt                                                                                 >  2:631329/1‑150 (MQ=255)
                                                                       gatcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGc                                                                             >  2:1188472/1‑150 (MQ=255)
                                                                       gatcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGc                                                                             >  2:731030/1‑150 (MQ=255)
                                                                        atcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCg                                                                            <  2:300329/150‑1 (MQ=255)
                                                                        atcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCg                                                                            >  1:268051/1‑150 (MQ=255)
                                                                        atcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCg                                                                            >  1:652525/1‑150 (MQ=255)
                                                                           aTTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTAc                                                                         >  1:681506/1‑150 (MQ=255)
                                                                            tttttttAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACt                                                                        >  2:1048027/1‑150 (MQ=255)
                                                                            tttttttAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACt                                                                        >  1:997930/1‑150 (MQ=255)
                                                                               ttttAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGc                                                                     <  2:719398/150‑1 (MQ=255)
                                                                                       gTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAg                                                             >  1:679229/1‑150 (MQ=255)
                                                                                        tCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGc                                                            >  1:1260329/1‑150 (MQ=255)
                                                                                         cAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCa                                                           >  1:1248810/1‑150 (MQ=255)
                                                                                                    gagagaTACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAg                                                >  1:2295349/1‑150 (MQ=255)
                                                                                                    gagagaTACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAg                                                >  2:1815653/1‑150 (MQ=255)
                                                                                                      gagaTACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCt                                              <  1:1232048/150‑1 (MQ=255)
                                                                                                       agaTACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTg                                             <  2:867432/150‑1 (MQ=255)
                                                                                                              cgtGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACa                                      <  1:2197881/148‑1 (MQ=255)
                                                                                                               ttGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAg                                     >  1:693992/1‑150 (MQ=255)
                                                                                                                  ccGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCAt                                  <  2:2290101/150‑1 (MQ=255)
                                                                                                                   cGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATc                                 >  2:2502412/1‑150 (MQ=255)
                                                                                                                   cGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCGTCATAAGCATCACGCAAAAGCTGCAAAACAGCATc                                 >  2:231186/1‑150 (MQ=255)
                                                                                                                        aTGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTcc                            >  2:1475571/1‑150 (MQ=255)
                                                                                                                         tgctgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTccc                           <  1:2403093/150‑1 (MQ=255)
                                                                                                                         tgctgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTccc                           <  2:1309388/150‑1 (MQ=255)
                                                                                                                          gctgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCg                          >  2:1931512/1‑150 (MQ=255)
                                                                                                                           ctgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCgg                         >  1:1435961/1‑150 (MQ=255)
                                                                                                                           ctgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCgg                         >  1:1435964/1‑150 (MQ=255)
                                                                                                                           ctgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCgg                         >  1:880158/1‑150 (MQ=255)
                                                                                                                            tgcCTCCACCTCATTAAGTTGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCATAGAAGTAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGa                        >  2:40111/1‑150 (MQ=255)
                                                                                                                             gcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGaa                       <  2:47042/150‑1 (MQ=255)
                                                                                                                                tCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCa                    >  2:2419167/1‑150 (MQ=255)
                                                                                                                                 ctttaagaTTAAGATGTAATATAAATACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAg                   >  1:1035683/8‑150 (MQ=39)
                                                                                                                                 ctttaagaTTAAGATGTAATATAAATACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAg                   >  2:143483/8‑150 (MQ=39)
                                                                                                                                 ctttaagaTTAAGATGTAATATAAATACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGAGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAg                   >  2:883182/8‑150 (MQ=38)
                                                                                                                                 ccACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAg                   >  2:1645972/1‑150 (MQ=255)
                                                                                                                                 acACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAATGGCACTATGACTCCAAAGAAGAAGGGTTATCCAACCGATACAAATTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCAGGAACCAg                   >  2:826580/2‑150 (MQ=255)
                                                                                                                                  cACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGc                  <  1:114958/150‑1 (MQ=255)
                                                                                                                                    ccTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCAt                <  2:760881/150‑1 (MQ=255)
                                                                                                                                    ccTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCAt                >  1:492755/1‑150 (MQ=255)
                                                                                                                                       cATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCaa             <  2:1551397/150‑1 (MQ=255)
                                                                                                                                             gATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTc       <  2:1672957/150‑1 (MQ=255)
                                                                                                                                              aTGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCg      >  2:1626891/1‑150 (MQ=255)
                                                                                                                                                 tATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCg   >  2:2292400/1‑150 (MQ=255)
                                                                                                                                                  aTTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGt  >  2:2199333/1‑150 (MQ=255)
                                                                                                                                                  aTTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGt  >  2:2199216/1‑150 (MQ=255)
                                                                                                                                                  aTTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGt  >  1:512847/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGT  >  NC_000913/2856685‑2856980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: