Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,856,836 T→A 5.1% intergenic (‑30/‑257) ygbA ← / → mutS uncharacterized protein/methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,856,8360TA 5.1% 429.3 / 10.1 117intergenic (‑30/‑257)ygbA/mutSuncharacterized protein/methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base T (66/45);  new base A (4/2);  total (70/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 120 total aligned reads displayed.

AACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGTTCGC  >  NC_000913/2856690‑2856984
                                                                                                                                                  |                                                                                                                                                    
aacaaacaACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATAtt                                                                                                                                                   >  2:1274189/1‑150 (MQ=255)
 acaaacaaCGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTa                                                                                                                                                  <  1:1379125/150‑1 (MQ=255)
   aaacaaCGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACa                                                                                                                                                >  1:832232/1‑150 (MQ=255)
   aaacaaCGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACa                                                                                                                                                <  2:55936/150‑1 (MQ=255)
      caacGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatct                                                                                                                                             <  2:1696741/150‑1 (MQ=255)
        acGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatctta                                                                                                                                           >  1:1551397/1‑150 (MQ=255)
         cGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaa                                                                                                                                          <  2:2543613/150‑1 (MQ=255)
         cGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaa                                                                                                                                          <  1:1861335/150‑1 (MQ=255)
          gCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTAATATAAATACatcttaat                                                                                                                                         <  1:952911/150‑1 (MQ=255)
           ccTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatc                                                                                                                                        >  2:1200358/1‑150 (MQ=255)
           ccTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatc                                                                                                                                        >  2:1352593/1‑150 (MQ=255)
             tCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCCTTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctt                                                                                                                                      >  1:1682600/1‑150 (MQ=255)
             tCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctt                                                                                                                                      >  2:2223641/1‑150 (MQ=255)
             tCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctt                                                                                                                                      >  1:369998/1‑150 (MQ=255)
             tCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctt                                                                                                                                      >  1:2317373/1‑150 (MQ=255)
              cGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctta                                                                                                                                     >  1:1912695/1‑150 (MQ=255)
              cGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACatcttaatctta                                                                                                                                     >  1:851954/1‑150 (MQ=255)
               gTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACAtcttaatcttaa                                                                                                                                    <  1:1219402/150‑1 (MQ=255)
                tAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTaaa                                                                                                                                   >  1:222891/1‑150 (MQ=255)
                tAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCGTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTaaa                                                                                                                                   >  1:2170507/1‑150 (MQ=255)
                   tGCTCCGGCTCCGCTGGCGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAggg                                                                                                                                <  1:671297/150‑1 (MQ=255)
                     ctccggctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCa                                                                                                                              >  1:777188/1‑150 (MQ=255)
                     ctccggctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCa                                                                                                                              >  2:464257/1‑150 (MQ=255)
                       ccggctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACt                                                                                                                            >  2:1232048/1‑150 (MQ=255)
                        cggctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTa                                                                                                                           >  2:595146/1‑150 (MQ=255)
                        cggatacgCAGACGCATGCCGGAATTTCTCTTGATAAAGATCGATCATTTTATTAATCTTAAGTTTTTAACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAATATTTATTTATATTACATCTTAATCTTAAATGGCACTa                                                                                                                           >  2:847152/7‑150 (MQ=255)
                         ggctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTAt                                                                                                                          <  2:1531569/150‑1 (MQ=255)
                           ctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGa                                                                                                                        <  1:589649/150‑1 (MQ=255)
                           ctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGa                                                                                                                        <  1:2537063/150‑1 (MQ=255)
                           ctccgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGa                                                                                                                        <  1:2537084/150‑1 (MQ=255)
                            tccgCGGACGCCAGCGGGCATTGCGCTTGATCCCGATCGATCATTTTTTGAATCGGCAGAATTTCACGAGAGATACGCTGGCCGGACATGCTGCCTCCACCTCATTAAGATCTCTATATATTACATCTTAATCTTAAAGGGCACTAAGAc                                                                                                                       >  2:2571786/1‑150 (MQ=255)
                              cgCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTc                                                                                                                     >  1:70605/1‑150 (MQ=255)
                                 tGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCaa                                                                                                                  <  1:457799/150‑1 (MQ=255)
                                  gACGCCTGCGGGCTTTTCGCTTGATATAGATCGATCATTTTTTTAATCGTCTGTTTTTCAGGAGATATTCGCGTGCCGGACATGCTGCCTCGACCTCAGTTAGTGGTATTTATTTTACATCTTAATCTTAAAGGGCACTATGACTCCaaa                                                                                                                 <  1:582644/150‑1 (MQ=255)
                                  gACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCaaa                                                                                                                 <  1:2436138/150‑1 (MQ=255)
                                     gCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAaagaa                                                                                                              <  2:494873/150‑1 (MQ=255)
                                     gCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAaagaa                                                                                                              <  2:1224507/150‑1 (MQ=255)
                                      ccTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAaagaag                                                                                                             >  2:1021343/1‑150 (MQ=255)
                                         gCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAaagaagaag                                                                                                          <  2:1813377/150‑1 (MQ=255)
                                          cGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAgg                                                                                                         <  1:1908297/150‑1 (MQ=255)
                                             gCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGtt                                                                                                      >  2:1333639/1‑150 (MQ=255)
                                               aTTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAg                                                                                                    <  1:2310065/150‑1 (MQ=255)
                                                  tCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCa                                                                                                 >  2:1346181/1‑150 (MQ=255)
                                                    gCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAAc                                                                                               >  1:890730/1‑150 (MQ=255)
                                                     cTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAAcc                                                                                              >  1:1897046/1‑150 (MQ=255)
                                                     cTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAAcc                                                                                              >  1:128905/1‑150 (MQ=255)
                                                      ttGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCCCGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCg                                                                                             <  1:192648/150‑1 (MQ=255)
                                                          tAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATAc                                                                                         <  1:1727155/150‑1 (MQ=255)
                                                          tAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATAc                                                                                         <  2:1482073/150‑1 (MQ=255)
                                                          tAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATAc                                                                                         >  2:424875/1‑150 (MQ=255)
                                                          tAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTACACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATAc                                                                                         <  1:1009706/150‑1 (MQ=255)
                                                              gatcgatcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAAtt                                                                                     >  2:631329/1‑150 (MQ=255)
                                                                  gatcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGc                                                                                 >  2:731030/1‑150 (MQ=255)
                                                                  gatcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGc                                                                                 >  2:1188472/1‑150 (MQ=255)
                                                                   atcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCg                                                                                <  2:1686423/150‑1 (MQ=255)
                                                                   atcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCg                                                                                <  2:300329/150‑1 (MQ=255)
                                                                   atcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCg                                                                                >  1:652525/1‑150 (MQ=255)
                                                                   atcATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCg                                                                                >  1:268051/1‑150 (MQ=255)
                                                                      aTTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTAc                                                                             >  1:681506/1‑150 (MQ=255)
                                                                       tttttttAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACt                                                                            >  2:1048027/1‑150 (MQ=255)
                                                                       tttttttAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACt                                                                            >  1:997930/1‑150 (MQ=255)
                                                                        ttttttAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACtt                                                                           <  1:2219492/150‑1 (MQ=255)
                                                                          ttttAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGc                                                                         <  2:719398/150‑1 (MQ=255)
                                                                                  gTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAg                                                                 >  1:679229/1‑150 (MQ=255)
                                                                                   tCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGc                                                                >  1:1260329/1‑150 (MQ=255)
                                                                                    cAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCa                                                               >  1:1248810/1‑150 (MQ=255)
                                                                                    cAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCa                                                               >  1:1248833/1‑150 (MQ=255)
                                                                                               gagagaTACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAg                                                    >  1:2295349/1‑150 (MQ=255)
                                                                                               gagagaTACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAg                                                    >  2:1815653/1‑150 (MQ=255)
                                                                                                  agaTACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTg                                                 <  2:867432/150‑1 (MQ=255)
                                                                                                      aCGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCaaa                                             <  2:2307320/150‑1 (MQ=255)
                                                                                                       cGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCaaaa                                            <  2:1513838/150‑1 (MQ=255)
                                                                                                             ccGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCAt                                      <  2:2290101/150‑1 (MQ=255)
                                                                                                              cGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATc                                     >  2:2502412/1‑150 (MQ=255)
                                                                                                              cGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCGTCATAAGCATCACGCAAAAGCTGCAAAACAGCATc                                     >  2:231186/1‑150 (MQ=255)
                                                                                                                   aTGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTcc                                >  2:1475571/1‑150 (MQ=255)
                                                                                                                    tgctgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTccc                               <  2:1309388/150‑1 (MQ=255)
                                                                                                                     gctgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCg                              >  2:1931512/1‑150 (MQ=255)
                                                                                                                      ctgcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCgg                             >  1:1435964/1‑150 (MQ=255)
                                                                                                                       tgcCTCCACCTCATTAAGTTGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCATAGAAGTAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGa                            >  2:40111/1‑150 (MQ=255)
                                                                                                                        gcCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGaa                           <  2:47042/150‑1 (MQ=255)
                                                                                                                           tCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCa                        >  2:2419167/1‑150 (MQ=255)
                                                                                                                            ctttaagaTTAAGATGTAATATAAATACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAg                       >  2:143483/8‑150 (MQ=39)
                                                                                                                            ctttaagaTTAAGATGTAATATAAATACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAg                       >  1:1035683/8‑150 (MQ=39)
                                                                                                                            ctttaagaTTAAGATGTAATATAAATACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGAGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAg                       >  2:883182/8‑150 (MQ=38)
                                                                                                                            ccACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAg                       >  2:1645972/1‑150 (MQ=255)
                                                                                                                            acACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAATGGCACTATGACTCCAAAGAAGAAGGGTTATCCAACCGATACAAATTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCAGGAACCAg                       >  2:826580/2‑150 (MQ=255)
                                                                                                                             tttaagaTTAAGATGTAATATAAATACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGc                      >  1:725721/7‑150 (MQ=255)
                                                                                                                             cACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGc                      <  1:114958/150‑1 (MQ=255)
                                                                                                                               ccTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCAt                    >  1:492755/1‑150 (MQ=255)
                                                                                                                               ccTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCAt                    <  2:760881/150‑1 (MQ=255)
                                                                                                                                      aaGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAAc             <  1:424875/150‑1 (MQ=255)
                                                                                                                                       aGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACt            >  2:208921/1‑150 (MQ=255)
                                                                                                                                        gATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTc           <  2:1672957/150‑1 (MQ=255)
                                                                                                                                         aTGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCg          >  2:1626891/1‑150 (MQ=255)
                                                                                                                                            tATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCg       >  2:2292400/1‑150 (MQ=255)
                                                                                                                                             aTTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGt      >  2:2199216/1‑150 (MQ=255)
                                                                                                                                             aTTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGt      >  1:512847/1‑150 (MQ=255)
                                                                                                                                                 atatTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGTTCGc  <  2:1080218/150‑1 (MQ=255)
                                                                                                                                                 atatTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGTTCGc  <  1:1048027/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTCACGAGAGATACGCTTGCCGGACATGCTGCCTCCACCTCATTAAGATGTATTTATATTACATCTTAATCTTAAAGGGCACTATGACTCCAAAGAAGAAGGGTTAGCCAACCGATACAATTTTGCGTACTTGCTTCATAAGCATCACGCAAAAGCTGCAAAACAGCATCTTTCCCGGAACCAGCATCAAGAACTCGCCGTTCGC  >  NC_000913/2856690‑2856984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: