BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,990,927	T→A	11.0%	pseudogene (374/396 nt)	ygeF →	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,990,927	0	T	A	11.0%	327.1 / 7.9	120	pseudogene (374/396 nt)	ygeF	pseudogene
Reads supporting (aligned to +/- strand): ref base T (58/45); new base A (2/11); total (61/59)
Fisher's exact test for biased strand distribution p-value = 6.95e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

TTATCTGCTATGAGCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGA  >  NC_000913/2990779‑2991070

ttatCTGCTATGAGCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTc                                                                                                                                                >  1:511842/1‑150 (MQ=255)

ttatCTGCTATGAGCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTc                                                                                                                                                >  1:511758/1‑150 (MQ=255)

  atCTGCTATGAGCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCAc                                                                                                                                              >  2:1083174/1‑150 (MQ=255)

   tCTGCTATGAGCATATATAAATTTTCGATAACAGATGATTTAATGTGTATGGCTGTAAATGTACTCATAATTCTGACGAATTTAGAGAATAATGTTGACCTGAATAAATTCACTACTATATGCTCAGACGTTCTATCCCCCCGCGTCAcc                                                                                                                                             >  2:1093157/1‑150 (MQ=255)

   tCTGCTATGAGCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCAcc                                                                                                                                             >  2:1965146/1‑150 (MQ=255)

   tCTGCTATGAGCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCAcc                                                                                                                                             >  2:1965268/1‑150 (MQ=255)

     tGCTATGAGCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAg                                                                                                                                           >  1:557817/1‑150 (MQ=255)

        tATGAGTATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAATACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGAACAGACGTACTATCCCACCGCGACACCAGACa                                                                                                                                        >  2:1394997/1‑150 (MQ=255)

           tatcATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACataa                                                                                                                                     <  2:2233450/147‑1 (MQ=255)

           gAGCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACataa                                                                                                                                     <  2:378447/150‑1 (MQ=255)

             gCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACataata                                                                                                                                   >  1:1033909/1‑150 (MQ=255)

             gCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACataata                                                                                                                                   >  2:708601/1‑150 (MQ=255)

                     aaaTTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGaaaaa                                                                                                                           >  2:1749318/1‑150 (MQ=255)

                        ttttCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAaaac                                                                                                                        <  2:815594/150‑1 (MQ=255)

                        ttttCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAaaac                                                                                                                        >  2:2526187/1‑150 (MQ=255)

                        ttttCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAaaac                                                                                                                        >  2:1048157/1‑150 (MQ=255)

                        ttttCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCCCGGCCCCAGACAGAAGAAAGAAAAaaac                                                                                                                        >  1:1226781/1‑150 (MQ=255)

                               tAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGAAGTTCTATCCCCCCGCGACACCAGAAATAATAAAGAAAAAAACAAACGaa                                                                                                                 >  2:2439145/1‑149 (MQ=255)

                                   aGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTc                                                                                                             >  2:1511409/1‑150 (MQ=255)

                                   aGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTc                                                                                                             >  1:53809/1‑150 (MQ=255)

                                   aGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTc                                                                                                             >  1:2102935/1‑150 (MQ=255)

                                     atgatTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTa                                                                                                           >  2:2140610/1‑150 (MQ=255)

                                         tttAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCt                                                                                                       >  1:1535854/1‑150 (MQ=255)

                                          ttAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCtt                                                                                                      >  2:692843/1‑150 (MQ=255)

                                           tAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTtc                                                                                                     <  2:1472279/150‑1 (MQ=255)

                                                 gTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCaaaa                                                                                               <  2:1945070/150‑1 (MQ=255)

                                                     tgatttaaattaaatAATAAACCTGAAAAAATTATATATTATTTTATACCTTATTAAATTCACTAATTTATGAACAGACTTTCTACCCCCCCGCTAAACCATAAATAATAAATAAAAAAAAAAACGACATTCTACCCTTCTCAAAAATcc                                                                                           <  1:1494042/145‑1 (MQ=255)

                                                     gggtGTAAATTAACTCATAAAACTGACAAAAATTGATATTTTTTTAGACCCTAATAAATAAAATATTTTATGCACTGAGTTTCTACCCCCCCGGTTCACCATAAATAATAAACAAAAAAACACACGACATTCTACCCTTCTCAAAAATcc                                                                                           <  1:1494041/147‑1 (MQ=255)

                                                      gCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATccc                                                                                          >  1:1572199/1‑150 (MQ=255)

                                                      gCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATccc                                                                                          >  1:615002/1‑150 (MQ=255)

                                                         gTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCtt                                                                                       >  1:134828/1‑150 (MQ=255)

                                                         gTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCACCtt                                                                                       >  2:2217834/1‑150 (MQ=255)

                                                          tAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCTTCACCAGACATAATAAATAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCttt                                                                                      <  2:1110036/150‑1 (MQ=255)

                                                          tAAATGAAATAATAAAACTGACCAAAATAGAGAATAATTTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCttt                                                                                      <  2:1543137/150‑1 (MQ=255)

                                                           aaaTTAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCtttt                                                                                     <  2:1852890/150‑1 (MQ=255)

                                                               gAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAAt                                                                                 >  1:1192515/1‑150 (MQ=255)

                                                               gAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAAt                                                                                 >  1:1192607/1‑150 (MQ=255)

                                                               gAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAAt                                                                                 >  1:1916867/1‑150 (MQ=255)

                                                                 aCTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAAttt                                                                               >  2:1522601/1‑150 (MQ=255)

                                                                 aCTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAAttt                                                                               >  1:803199/1‑150 (MQ=255)

                                                                  cTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAAtttt                                                                              <  2:372484/150‑1 (MQ=255)

                                                                  cTCATAAAACTGACAAAAATAGAGAATAATGTATACCTTAATAAATTCACTACTATATGCACAGACGTTCTACCCCCCCGCGTCACCAGAAATAATAAACAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAAtttt                                                                              <  2:502998/150‑1 (MQ=255)

                                                                   tCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTc                                                                             <  2:2535829/150‑1 (MQ=255)

                                                                    aaTAAAACTGACAAAAATACACAATATTTTAGACATTAATAAATACACTACTATATCAACAGACGTTCTACCCCCCCGCGACACCACACATAATAAACAAAAAAACACACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCa                                                                            <  1:1465971/149‑1 (MQ=255)

                                                                              aCCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAAt                                                                  >  1:2295696/1‑150 (MQ=255)

                                                                              aCCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATAGAAAAAAt                                                                  >  1:2295672/1‑150 (MQ=255)

                                                                              aCCAAAATAGAGAATAATGTAGACATGAATAAAATCACAACTATAAGCACAGACGTACTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACACACGACATACTACCCTACTCAAAAATCCCATTTTTAATTTTCAATGAAAAAAt                                                                  >  1:2357210/1‑150 (MQ=255)

                                                                                cAAAATAGAGAATAATTTAGACCTCAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGACACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATgg                                                                <  1:2061699/150‑1 (MQ=255)

                                                                                aaaaaTAGAGAATAATTTATACCTGAATAAATTCACTACTATATGCACAGACTTTCTACCCCCCCGCGACACCACACATAATAAACAAAAAAACACACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATgg                                                                <  1:2061847/149‑1 (MQ=255)

                                                                                  aaaTAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCa                                                              >  2:1638731/1‑150 (MQ=255)

                                                                                       tataataatGTAGACCTTAATAAATTCACTACTATATGAACTGAGGTTCTAACCCCCCGCGTCACCAGACATAATAAAGAAAAAAAAACACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGa                                                         <  1:1265274/147‑1 (MQ=255)

                                                                                       tagaTTAATTTAGACATGAATAATTTCAATAATATATGAACAGACGTTCTATCCCCCCGCGTCACCATAAATAATAAACAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGa                                                         <  1:1023884/149‑1 (MQ=255)

                                                                                       gagaATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAACAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGa                                                         <  2:277272/150‑1 (MQ=255)

                                                                                       cagaATAATGTAGACCTCAAAAAATACACAACTATATGCACAGACGTTCTATCCCCCCGCGTCACCACAAATAATAAATAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGa                                                         <  1:1355206/149‑1 (MQ=255)

                                                                                         taatattTTAGACCTGAATAAATTCACTACTATATCCACAGACGTTCTAACCCCCCGCGCCACCAGACATAATAAACAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATa                                                       <  1:2421395/149‑1 (MQ=255)

                                                                                         gaATAATTTATACCTTAATAAATTCACTACTATATGAACAGCCGTTCTACCCCCCCGCGACACCAGAAAAAAAAAACAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATa                                                       <  1:2499954/150‑1 (MQ=255)

                                                                                            tattGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGAAATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTa                                                    <  1:1083174/147‑1 (MQ=255)

                                                                                             attGTAGACCTTAATAAATTCACTACTATATGCACAGACGTTATATCCCCCCGCGCAACCACACATAATAAACAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTAt                                                   <  2:474087/148‑1 (MQ=255)

                                                                                              atGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATg                                                  <  2:2039285/150‑1 (MQ=255)

                                                                                                gTAGACCTGAATAAATACACTACTATATGCACAGACTTTCTACCCCCCCGCGACACCATACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGaa                                                <  1:1846834/150‑1 (MQ=255)

                                                                                                  aGACCTCAATAAATTCACTACTATATGCACAGACGTTATAACCCCCCGCTTCACCACACATAATAAACAAAAAAACACACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATc                                              <  1:710924/150‑1 (MQ=255)

                                                                                                    aCCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCa                                            <  2:811965/150‑1 (MQ=255)

                                                                                                    aCCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCa                                            <  2:811989/150‑1 (MQ=255)

                                                                                                      cttaaTAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAgg                                          <  1:2145007/147‑1 (MQ=255)

                                                                                                      cttaaTAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACAACAAATAATAAACAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAgg                                          <  1:2519432/147‑1 (MQ=255)

                                                                                                      cttaaTAAATTCACTACTATATGCACAGACGTTCTACCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAgg                                          <  1:2246793/147‑1 (MQ=255)

                                                                                                      cTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAgg                                          >  1:785380/1‑150 (MQ=255)

                                                                                                         aaTAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCa                                       >  1:1116262/1‑150 (MQ=255)

                                                                                                         aaTAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCa                                       >  1:1116271/1‑150 (MQ=255)

                                                                                                          aTAAATTCAATACTATATGCACATACGTTATATCCCCCCGCTTCACCATACATAATAAAGAAAAAAAAAAACGACATTCTAACCTTCTCAAAAATCCCCTTTTTAATTTTCATTTAAAAAATGGCAATTGATACTATGAATCCAGGTCAt                                      <  2:2439968/150‑1 (MQ=255)

                                                                                                           tAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATa                                     >  1:1296943/1‑150 (MQ=255)

                                                                                                              aTTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATacac                                  <  2:1072147/150‑1 (MQ=255)

                                                                                                              aTTCACTACTATATGCACAGACGTTCTATCCCCCCGCGACACCAGAAATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATacac                                  <  2:2231982/150‑1 (MQ=255)

                                                                                                              aTTCACTACTATATGCACAGACGTTCTACCCCCCCGCTTCACCAGAAATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATacac                                  <  2:2231975/150‑1 (MQ=255)

                                                                                                                  actactATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTa                              >  1:149892/1‑150 (MQ=255)

                                                                                                                    tactaTATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAg                            <  1:170245/150‑1 (MQ=255)

                                                                                                                    tactaTATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAg                            >  1:942280/1‑150 (MQ=255)

                                                                                                                    tactaTATGCACAGACGTTCTATCCCCCCGCGTCACCAGAAATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAg                            <  2:2564693/150‑1 (MQ=255)

                                                                                                                          atGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAATGCAttt                      >  1:886222/1‑150 (MQ=255)

                                                                                                                          atGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGTCAttt                      >  1:886206/1‑150 (MQ=255)

                                                                                                                          atGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCAttt                      >  1:315089/1‑150 (MQ=255)

                                                                                                                            gCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTAt                    <  2:557817/150‑1 (MQ=255)

                                                                                                                             cacaGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATg                   >  2:644827/1‑150 (MQ=255)

                                                                                                                                aGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTaa                <  2:374023/150‑1 (MQ=255)

                                                                                                                                aGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTaa                >  1:108809/1‑150 (MQ=255)

                                                                                                                                    gTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTc            <  1:2504237/150‑1 (MQ=255)

                                                                                                                                    gTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTc            <  2:1628845/150‑1 (MQ=255)

                                                                                                                                     ttCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCg           >  1:215893/1‑150 (MQ=255)

                                                                                                                                      tCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGt          >  1:2211181/1‑150 (MQ=255)

                                                                                                                                      tCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCATTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGt          <  1:796195/150‑1 (MQ=255)

                                                                                                                                      tCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCATTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGt          <  1:983416/150‑1 (MQ=255)

                                                                                                                                      tCTATCCCCCCGCGACACCATAAATAATAAACAAAAAAACAAACGACATTCTACCATTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGt          <  1:451224/150‑1 (MQ=255)

                                                                                                                                      tCTAACCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCATTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGt          <  1:879641/150‑1 (MQ=255)

                                                                                                                                       cTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGtc         >  1:1049551/1‑149 (MQ=255)

                                                                                                                                       cTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTa         >  2:282894/1‑150 (MQ=255)

                                                                                                                                        tATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTaa        >  2:1675478/1‑150 (MQ=255)

                                                                                                                                        tATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTaa        >  2:949405/1‑150 (MQ=255)

                                                                                                                                         aTCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTTATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAAt       >  2:2245977/1‑150 (MQ=255)

                                                                                                                                         aTCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAAt       >  1:438272/1‑150 (MQ=255)

                                                                                                                                              cccGCGCCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAACTCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGa  <  1:477833/150‑1 (MQ=255)

TTATCTGCTATGAGCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGA  >  NC_000913/2990779‑2991070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`