Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,057,349 C→G 5.1% L354L (CTG→CTC)  serA ← D‑3‑phosphoglycerate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,057,3490CG 5.1% 585.5 / 17.2 158L354L (CTG→CTC) serAD‑3‑phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (70/80);  new base G (4/4);  total (74/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 158 total aligned reads displayed.

TGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCAGGC  >  NC_000913/3057201‑3057497
                                                                                                                                                    |                                                                                                                                                    
tGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCa                                                                                                                                                     <  1:962613/150‑1 (MQ=255)
   tACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAgcg                                                                                                                                                  >  1:2522584/1‑150 (MQ=255)
   tACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCAGCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAgcg                                                                                                                                                  >  1:11111/1‑150 (MQ=255)
     cccGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCa                                                                                                                                                <  2:1710964/150‑1 (MQ=255)
     cccGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCa                                                                                                                                                >  1:1541495/1‑150 (MQ=255)
     cccGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCa                                                                                                                                                <  2:669384/150‑1 (MQ=255)
     cccGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCa                                                                                                                                                <  2:94448/150‑1 (MQ=255)
           aTAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGc                                                                                                                                          <  2:1153609/150‑1 (MQ=255)
            tAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCa                                                                                                                                         <  1:1236703/150‑1 (MQ=255)
            tAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCa                                                                                                                                         <  2:945496/150‑1 (MQ=255)
                tttCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTGAGCGCAGTTAGCACGcc                                                                                                                                     >  2:1903208/1‑150 (MQ=255)
                tttCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTGAGCGCAGTTAGCACGcc                                                                                                                                     >  1:1932031/1‑150 (MQ=255)
                tttCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGcc                                                                                                                                     >  2:1174424/1‑150 (MQ=255)
                 ttCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGccc                                                                                                                                    <  1:2309646/150‑1 (MQ=255)
                      tGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACg                                                                                                                               <  1:1641777/150‑1 (MQ=255)
                        ccTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGt                                                                                                                             >  2:1589171/1‑150 (MQ=255)
                           gcagcaCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGtttt                                                                                                                          <  1:621185/150‑1 (MQ=255)
                           gCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGtttt                                                                                                                          <  1:893328/150‑1 (MQ=255)
                           gCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGtttt                                                                                                                          <  1:2048172/150‑1 (MQ=255)
                            cAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTc                                                                                                                         >  1:448730/1‑150 (MQ=255)
                              gcgcTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGt                                                                                                                       >  1:2012832/1‑150 (MQ=255)
                              gcgcTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGt                                                                                                                       <  2:2075019/150‑1 (MQ=255)
                              gcgcTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGt                                                                                                                       <  2:2305322/150‑1 (MQ=255)
                                  tttttCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACCTAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGAt                                                                                                                   <  1:2428138/150‑1 (MQ=255)
                                        ggCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCAt                                                                                                             <  2:1426434/150‑1 (MQ=255)
                                        ggCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCAt                                                                                                             <  2:2196716/150‑1 (MQ=255)
                                          cAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCa                                                                                                           >  2:1613609/1‑150 (MQ=255)
                                          cAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCa                                                                                                           >  1:145174/1‑150 (MQ=255)
                                          cAACGTCGTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCa                                                                                                           <  2:608206/150‑1 (MQ=255)
                                             cGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAgac                                                                                                        >  1:1703618/1‑150 (MQ=255)
                                               tCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAgacga                                                                                                      <  1:94545/150‑1 (MQ=255)
                                               tCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAgacga                                                                                                      <  2:222738/150‑1 (MQ=255)
                                               tCTTCGTCGGCGTCAATATCAATAACCACCTAACCCCTCGGGGCGGAAGTGTGCAGATATGGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACTCCCGGACGGTTTTCGTGGATGTGCATCAgacga                                                                                                      <  1:475737/150‑1 (MQ=255)
                                                cTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAgacgac                                                                                                     >  2:990891/1‑150 (MQ=255)
                                                cTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAgacgac                                                                                                     >  2:923949/1‑150 (MQ=255)
                                                        gCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCg                                                                                             >  1:1614660/1‑150 (MQ=255)
                                                          ttCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTg                                                                                           >  1:1046835/1‑150 (MQ=255)
                                                          ttCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTg                                                                                           >  1:118342/1‑150 (MQ=255)
                                                          ttCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTg                                                                                           >  1:118364/1‑150 (MQ=255)
                                                          ttCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTg                                                                                           >  1:2329038/1‑150 (MQ=255)
                                                          ttCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTg                                                                                           >  2:2014111/1‑150 (MQ=255)
                                                          ttCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTg                                                                                           >  1:2125787/1‑150 (MQ=255)
                                                          ttCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTg                                                                                           >  1:819767/1‑150 (MQ=255)
                                                          ttCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTGTTCGTGGATGTGCATCAGACGACGCCCACCGTg                                                                                           >  2:2045738/1‑150 (MQ=255)
                                                             aatatcaataACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAg                                                                                        <  2:15658/150‑1 (MQ=255)
                                                               tatcaataACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTg                                                                                      <  2:2093205/150‑1 (MQ=255)
                                                                atcaataACCCCCTAACCCATCTGGGCGGTAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGGTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTgg                                                                                     <  2:1302999/150‑1 (MQ=255)
                                                                       aCCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCgag                                                                              >  2:774772/1‑150 (MQ=255)
                                                                        ccACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCgaga                                                                             <  2:1103570/150‑1 (MQ=255)
                                                                         cacaTAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGAc                                                                            <  2:2344282/150‑1 (MQ=255)
                                                                         cacaTAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGAc                                                                            <  2:249108/150‑1 (MQ=255)
                                                                         cacaTAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGAc                                                                            >  1:1350191/1‑150 (MQ=255)
                                                                         cacaTAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGAc                                                                            <  2:1754279/150‑1 (MQ=255)
                                                                         cacaTAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGAc                                                                            >  1:1230212/1‑150 (MQ=255)
                                                                         cacaGACCCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGAc                                                                            <  2:1754436/150‑1 (MQ=255)
                                                                          acaTAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACt                                                                           >  2:2415436/1‑150 (MQ=255)
                                                                               aCCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCgg                                                                      <  1:2371228/150‑1 (MQ=255)
                                                                                          cGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCg                                                           <  1:635206/150‑1 (MQ=255)
                                                                                          cGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCg                                                           <  1:635238/150‑1 (MQ=255)
                                                                                           ggAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGc                                                          >  1:866549/1‑150 (MQ=255)
                                                                                                tttGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCagaga                                                     >  2:1220417/1‑150 (MQ=255)
                                                                                                tttGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCagaga                                                     >  2:2387296/1‑150 (MQ=255)
                                                                                                  tGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGc                                                   >  1:2175914/1‑150 (MQ=255)
                                                                                                   gCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCg                                                  >  1:1960594/1‑150 (MQ=255)
                                                                                                   gCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCg                                                  >  1:1669449/1‑150 (MQ=255)
                                                                                                     aGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTGAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGtt                                                <  1:1903208/150‑1 (MQ=255)
                                                                                                     aGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTGAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGtt                                                <  2:1932031/150‑1 (MQ=255)
                                                                                                     aGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGtt                                                <  2:2184213/150‑1 (MQ=255)
                                                                                                     aGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGtt                                                <  2:1929508/150‑1 (MQ=255)
                                                                                                      gATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTg                                               <  1:409916/150‑1 (MQ=255)
                                                                                                       atatTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGa                                              >  2:928211/1‑150 (MQ=255)
                                                                                                              gcggcgATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTg                                       <  1:1680011/150‑1 (MQ=255)
                                                                                                              gcggcgATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTg                                       <  1:718102/150‑1 (MQ=255)
                                                                                                               cggcgATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGt                                      <  1:1492059/150‑1 (MQ=255)
                                                                                                                ggcgATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTc                                     <  1:1896213/150‑1 (MQ=255)
                                                                                                                 gcgATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCa                                    <  2:1244729/150‑1 (MQ=255)
                                                                                                                    aTGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGaa                                 <  1:158239/150‑1 (MQ=255)
                                                                                                                       ttGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATAc                              >  2:2238144/1‑150 (MQ=255)
                                                                                                                       ttGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATAc                              >  2:490089/1‑150 (MQ=255)
                                                                                                                         gACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACtt                            <  1:576046/150‑1 (MQ=255)
                                                                                                                          aCGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTg                           >  1:1749871/1‑150 (MQ=255)
                                                                                                                              ccTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCa                       <  1:2563860/150‑1 (MQ=255)
                                                                                                                              ccTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCa                       >  2:871516/1‑150 (MQ=255)
                                                                                                                                tGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAAt                     <  1:2303027/150‑1 (MQ=255)
                                                                                                                                 gCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAAtt                    <  2:2329038/150‑1 (MQ=255)
                                                                                                                                    cGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTAc                 <  2:808377/150‑1 (MQ=255)
                                                                                                                                    cGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTAc                 <  2:392089/150‑1 (MQ=255)
                                                                                                                                    cGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTAc                 <  2:392100/150‑1 (MQ=255)
                                                                                                                                     ggCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTAcc                >  2:1655655/1‑150 (MQ=255)
                                                                                                                                     ggCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTAcc                >  1:346780/1‑150 (MQ=255)
                                                                                                                                      gCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTAccc               >  2:1055785/1‑150 (MQ=255)
                                                                                                                                      gCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTAccc               >  2:1721197/1‑150 (MQ=255)
                                                                                                                                      gCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTAccc               >  1:2111627/1‑150 (MQ=255)
                                                                                                                                            aTTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACt         >  1:1990095/1‑150 (MQ=255)
                                                                                                                                                tGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCa     >  2:503885/1‑150 (MQ=255)
                                                                                                                                                tGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCa     >  2:294627/1‑150 (MQ=255)
                                                                                                                                                  ttCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCAgg   <  2:1541495/150‑1 (MQ=255)
                                                                                                                                                  ttCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCAgg   >  1:1562222/1‑150 (MQ=255)
                                                                                                                                                   tCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCAGGc  <  2:1465812/150‑1 (MQ=255)
                                                                                                                                                   tCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCAGGc  >  1:943146/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCAGGC  >  NC_000913/3057201‑3057497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑