Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,270,143 C→T 9.5% intergenic (+541/+73) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1430CT 9.5% 292.4 / 15.4 118intergenic (+541/+73)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base C (63/40);  new base T (2/9);  total (67/51)
Fisher's exact test for biased strand distribution p-value = 8.91e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

GACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAG  >  NC_000913/3270000‑3270292
                                                                                                                                               |                                                                                                                                                      
gACCGATAAACCGCTTTCTTTTGGGTATAGTGACGTCGACAGACATTCAACTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGTGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTg                                                                                                                                                  <  1:2052950/150‑1 (MQ=11)
    gATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACa                                                                                                                                              <  2:706162/150‑1 (MQ=37)
    gATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACa                                                                                                                                              <  2:706137/150‑1 (MQ=37)
    gATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACa                                                                                                                                              >  2:1600330/1‑150 (MQ=37)
    gATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACa                                                                                                                                              <  2:373395/150‑1 (MQ=37)
    gATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTAAAGCGTCGTGGaaa                                                                                                                                              >  2:298428/1‑148 (MQ=12)
         tccGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcat                                                                                                                                         <  2:991319/149‑1 (MQ=37)
         gCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAACCACGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGtcaa                                                                                                                                         >  2:2180576/1‑149 (MQ=255)
          ccGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAACCGGGTTTTTACGTAAAACAGGTGAAACTGAACGATAAGCCGCTTTCTTTTGGGTATAGCGACGAGGAAAGtcaaa                                                                                                                                        >  2:1285024/1‑148 (MQ=11)
          ccGCGTCCTTTTGGGTATAGTCTCGTGGACAGACATCCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACCTAAACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcatt                                                                                                                                        <  2:1434836/150‑1 (MQ=11)
           cgatctctttTGGCGTTTGTGTCGTGGACAGACTCTCAACTTTCTGCCACTCCACAATCAAAAACCCGCCGCATCGGGTTTTTACGTAACCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattc                                                                                                                                       <  2:1900924/145‑1 (MQ=11)
            gCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattca                                                                                                                                      <  1:1134774/150‑1 (MQ=37)
             ctttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattcat                                                                                                                                     <  1:568548/150‑1 (MQ=37)
               ttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGCCAGgcaatcaacc                                                                                                                                   >  2:1971453/1‑147 (MQ=11)
                  ttttGGGTATAGTGTCGTGGAAAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGACGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGAAAAGCCGCGTTCATTTGGGTATAGCGTCGTGTCCAGTCAatcatcgtaa                                                                                                                                >  2:50669/1‑146 (MQ=11)
                        gTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgt                                                                                                                                                   <  1:60115/125‑1 (MQ=17)
                        gTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAACGTTAAGcccc                                                                                                                          >  2:1572434/1‑150 (MQ=11)
                          ataGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTc                                                                                                                        <  1:2363237/150‑1 (MQ=37)
                               gtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                                                                                   <  1:1071636/150‑1 (MQ=37)
                                tCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAg                                                                                                                  >  2:846805/1‑150 (MQ=37)
                                tCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTACAAAAg                                                                                                                  >  2:1470491/1‑150 (MQ=255)
                                tCGTGGACAGTCATTCATCTTTCTGCCCCTACAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTACATCAACTTTCTGACCCTCCAAAAg                                                                                                                  >  1:2231759/1‑150 (MQ=11)
                                tCGTGGACAGTCATTCATCTTACTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTATCTGTTGGGTATAGCGTCGTGGGCAGTCATTCATCTTTCTGCCCCTCCAAAAg                                                                                                                  >  2:1557024/1‑150 (MQ=255)
                                         gTCCTTCATCTTTCTGCCCCTCCAAAAGCATAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAccc                                                                                                        <  2:1736767/150‑1 (MQ=255)
                                         gTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAccc                                                                                                        <  1:1462133/150‑1 (MQ=37)
                                          tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                                                                                       >  1:1649490/1‑150 (MQ=37)
                                          tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                                                                                       >  1:850771/1‑150 (MQ=37)
                                          tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAACccg                                                                                                       >  1:1851536/1‑150 (MQ=37)
                                          tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATATTTATGCCCCTCCAAAATCAAAAACccg                                                                                                       >  1:1851775/1‑150 (MQ=255)
                                            attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                                                                                     >  1:1791032/1‑150 (MQ=37)
                                             ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgccg                                                                                                    >  1:2101297/1‑150 (MQ=37)
                                                 tCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTc                                                                                                                        <  1:1775486/127‑1 (MQ=17)
                                                   tttCTGCCCCTCCAATAGCTAAAACCCGCCGTATCGTGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                              <  2:1212899/150‑1 (MQ=11)
                                                   tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCTTGGACAGTCAATCATATTTCAGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                              >  2:1238644/1‑150 (MQ=255)
                                                   tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                              >  1:643753/1‑150 (MQ=37)
                                                   tttCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACAGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAATCATTAAAATTACTGAACAACCAAAAGCAAAAACCAGCCGAAGCgg                                                                                              >  2:1998703/1‑150 (MQ=11)
                                                   gttCGGCCCCGCCACACGCAAAAACCCGCGGTCGCGGGTTTTTACGTTCTCCCGGTGAACCTGACCGATAAGCCGCTTTCTTTTGGGTATCGGGTCGTGGACAGTCTTTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                              <  1:913842/149‑1 (MQ=11)
                                                          cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                       >  1:283590/1‑150 (MQ=37)
                                                          cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGAGTCGTGGAAAGTCAATCATCTTACAGCCCCTACAAAAGCAAAAACCCGCCGAAGCGGGgttttt                                                                                       >  2:1413704/1‑149 (MQ=11)
                                                          cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGTAGAGCGTCGTGGACAGTCCAGCATCATTCTGCCCCTCCAACAGCAAAAACCCGCCGAAGCgggggtgta                                                                                       >  2:1413681/1‑144 (MQ=11)
                                                           ccTCCACCAGCACAAACCCGCCGGATCGGTTTATTACGTAAACCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                      <  2:1332810/150‑1 (MQ=11)
                                                           ccTCCAAAATCACAAACCCGCCGCAGCGGTTTATTACGTACAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                      <  2:20305/150‑1 (MQ=11)
                                                           ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCGCCTCCAAAAGCAAAAACACGCCGAAGCGGGGTGTTAc                                                                                      >  1:447419/1‑150 (MQ=255)
                                                           ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                      >  1:447316/1‑150 (MQ=37)
                                                            cTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                      <  2:85113/150‑1 (MQ=255)
                                                              ccAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTa                                                                                   >  2:1291508/1‑150 (MQ=37)
                                                                            ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt                                                                     >  1:1210279/1‑150 (MQ=37)
                                                                            ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt                                                                     >  1:520014/1‑150 (MQ=37)
                                                                            ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGtaaat                                                                     >  1:404573/1‑148 (MQ=255)
                                                                            ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAACTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt                                                                     >  2:927916/1‑150 (MQ=37)
                                                                              gccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATAAGGTGAAACTGa                                                                   >  1:951669/1‑150 (MQ=37)
                                                                              gccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTGTTACGTAAATCAGGTGAAACTGa                                                                   >  1:951686/1‑150 (MQ=37)
                                                                              gccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGa                                                                   >  1:2100758/1‑150 (MQ=37)
                                                                               ccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAc                                                                  >  2:2476319/1‑150 (MQ=37)
                                                                                      gggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTGATCTTTCTGCCCCTCCAATAGCAAATACCCGCCGTAGCGGGTTTTTAGGTTAATCAGGTGTAAGTGTCCGATAAg                                                           >  2:1806138/1‑150 (MQ=11)
                                                                                       ggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGc                                                          >  1:406148/1‑150 (MQ=37)
                                                                                       ggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTTACCGATAAGc                                                          >  1:406151/1‑150 (MQ=255)
                                                                                               cGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCt                                                  >  2:395419/1‑150 (MQ=17)
                                                                                                gTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTg                                                 >  1:146788/1‑150 (MQ=255)
                                                                                                   catcAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCg                                              <  2:1503833/147‑1 (MQ=255)
                                                                                                    atcAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt                                             <  2:835966/148‑1 (MQ=255)
                                                                                                    atcAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt                                             <  1:1358803/148‑1 (MQ=255)
                                                                                                    aaccggTGAAACTGACCGATAAGCCGCTTTCTTTTGTGGATAGCGTCGCCGACAGACATTCATATTTATGCCCCTCAAAAAGCATAAACCCGCCGTAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt                                             <  1:46481/146‑1 (MQ=14)
                                                                                                    aacAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGTTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt                                             <  2:362664/148‑1 (MQ=255)
                                                                                                    aCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt                                             >  2:1468596/1‑150 (MQ=255)
                                                                                                    aCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAGTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt                                             <  2:2101297/150‑1 (MQ=255)
                                                                                                      cAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTgg                                           >  2:1556952/1‑150 (MQ=255)
                                                                                                         gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACa                                        <  1:1453273/150‑1 (MQ=255)
                                                                                                          tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAg                                       >  2:2060364/1‑150 (MQ=255)
                                                                                                            aaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTATTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtc                                     <  2:75696/150‑1 (MQ=255)
                                                                                                              aCTGACAGATAATCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATAAGGGGAAACTGACCGAAAAGCCGGGGTATGTCGTGGACAGtcat                                   >  2:2370433/1‑150 (MQ=14)
                                                                                                                tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                          <  1:1518581/93‑1 (MQ=33)
                                                                                                                    cGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAACTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCCGGTTTTTACGTAAATCAGGTGAAACTGACCGATAACCCCGGTTCTGTCGTGGACAGTCATGCATCt                             >  2:1299927/1‑150 (MQ=17)
                                                                                                                     gATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTa                            <  2:2099510/150‑1 (MQ=255)
                                                                                                                     gATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTa                            <  1:1237287/150‑1 (MQ=255)
                                                                                                                     gATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTa                            <  1:1291508/150‑1 (MQ=255)
                                                                                                                     gATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTa                            <  1:2513205/150‑1 (MQ=255)
                                                                                                                         aGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCAAGGcc                        >  2:1928188/1‑150 (MQ=255)
                                                                                                                          gCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                       <  2:1434673/150‑1 (MQ=255)
                                                                                                                            cGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAATCAAAAAACCCCCCAAGCGGGTTTTTAAGTAAAAAAGGTGAAAAAGAACCATAAGCCGGGTTATTTCGTGGACACTAAATCAACTATGCCacc                     >  1:405195/1‑148 (MQ=9)
                                                                                                                             gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAATACCCGCCGAAGCGGGTTTTTTCGTAAATGAGGTGTAACTGAGCGTTAATCCGGGTTCTGTCGTGGACAGTCATTGTTCTAGGgcagca                    >  2:626310/1‑150 (MQ=17)
                                                                                                                             gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAt                                                                                <  1:600335/90‑1 (MQ=35)
                                                                                                                              ctttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCaa                   <  1:306663/150‑1 (MQ=255)
                                                                                                                              ctttctttTGGGTATAGCGTCGTGGACAGTCAATCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACCGTCATTCATCTAGGCCAGCaa                   >  1:1336984/1‑150 (MQ=255)
                                                                                                                               tttctttTGGGTATAGCGTCGTGGGCAGTCAATAATCTTTCTGCCCCACCAAAACCAAAAAACCCCCCAAGCCGGTTTTTACGTAAATAAGGTTAAAATGACCGATAAGCCGGGTTCAGTCGTGGACAGTCAATAAACTAGGCCAGCaaa                  >  1:992288/1‑149 (MQ=255)
                                                                                                                               tttctttAGGGTATAGCGTCGTGGACAGTCATACAACTATCTGCCCCTCCAAAAGCAAAAACCCCCCGAAGCGGGTTTTTACGTAAAACAGGTGAAAAAGACCGATAAGCCGGGGTCAGTCGAGGACAGTCATTCATCGAGGCCACCaaa                  >  1:48133/1‑149 (MQ=17)
                                                                                                                                ttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATc                 <  1:1111155/150‑1 (MQ=255)
                                                                                                                                ttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATc                 <  1:2392133/150‑1 (MQ=255)
                                                                                                                                ttctttTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGTAAAGACAATAATAAAGGCCATCaaac                 >  2:275823/1‑148 (MQ=14)
                                                                                                                                ttctttGGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAACAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATc                 <  1:1111235/150‑1 (MQ=255)
                                                                                                                                  ctttTGGGTATAGCGTCGTGGACAGTCATTCATCATTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGc               <  1:846808/150‑1 (MQ=255)
                                                                                                                                  cgtctGGGGATAGCGGCGTGGACCGTCCGTCATCTTTCTGCCCCGCCCCCAGCAAACACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATACGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGc               <  2:1301000/146‑1 (MQ=21)
                                                                                                                                   ttttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCt              >  2:1065776/1‑150 (MQ=255)
                                                                                                                                        ggTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTg         >  2:1103197/1‑150 (MQ=255)
                                                                                                                                        ggTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTg         >  2:1181000/1‑150 (MQ=255)
                                                                                                                                        ggTATAGCGTCGTGGACAGTCATTCAAATTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCCAACGCTCCCTg         >  1:105007/1‑150 (MQ=18)
                                                                                                                                          tataGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCATAAGTATAAACCCGCCGCAGCGGTTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGc       <  2:1417115/150‑1 (MQ=21)
                                                                                                                                            taGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTc     >  2:2583205/1‑150 (MQ=255)
                                                                                                                                               cgtcgtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAag  >  2:676356/1‑150 (MQ=255)
                                                                                                                                               cgtcgtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAag  >  2:2049751/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                      
GACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAG  >  NC_000913/3270000‑3270292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: