Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,368,189 A→G 5.4% A121A (GCA→GCG yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,1890AG 5.4% 377.8 / 8.3 111A121A (GCA→GCGyhcGDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
Reads supporting (aligned to +/- strand):  ref base A (62/43);  new base G (2/4);  total (64/47)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

Only 100 of 116 total aligned reads displayed.

AAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCG  >  NC_000913/3368040‑3368338
                                                                                                                                                     |                                                                                                                                                     
aaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCa                                                                                                                                                       >  1:418051/1‑150 (MQ=255)
  gtctttatttaTAATGTATAATCTTGGGTTCTATTCAAAAAACTTACGACAACTGCGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCTGCAGACGGTGTCTGCGGa                                                                                                                                                     <  2:1397403/147‑1 (MQ=255)
   gATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAACTGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCTTTGGGAATTCTGCAGACGGTGTCTGCGGaa                                                                                                                                                    <  2:1361822/150‑1 (MQ=255)
   gATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGaa                                                                                                                                                    <  2:727679/150‑1 (MQ=255)
   gATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGaa                                                                                                                                                    <  2:18939/150‑1 (MQ=255)
   gATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGaa                                                                                                                                                    <  1:552498/150‑1 (MQ=255)
   gATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGaa                                                                                                                                                    <  1:108600/150‑1 (MQ=255)
   gATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGaa                                                                                                                                                    <  2:544246/150‑1 (MQ=255)
   gATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGaa                                                                                                                                                    <  1:2445066/150‑1 (MQ=255)
    aTTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCCTTGGGAATTCTGCAGACGGTGTCTGCGGAAt                                                                                                                                                   <  2:577837/150‑1 (MQ=255)
           tCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATc                                                                                                                                            >  2:2195203/1‑150 (MQ=255)
           tCTAAGGTATAACAGTGGGTTATCTGCAAAAAACTTACGCCAAATGAGGCTTTTTAAAATCTTTTTTCAAAATGTTGAAATTCACCAGAAAAAGTAAGTCGACTTAACAACAATAGGAAATCCGCAGACACCGTATTCAGAATTCCCaac                                                                                                                                            >  2:2195200/1‑148 (MQ=255)
              aaGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGc                                                                                                                                         >  1:1308688/1‑150 (MQ=255)
              aaGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGc                                                                                                                                         >  2:1174285/1‑150 (MQ=255)
              aaGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGc                                                                                                                                         >  2:710595/1‑150 (MQ=255)
              aaGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGc                                                                                                                                         >  2:1174364/1‑150 (MQ=255)
              aaGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCAATCGGc                                                                                                                                         >  1:1416651/1‑150 (MQ=255)
                ggTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCaa                                                                                                                                       >  2:553964/1‑150 (MQ=255)
                  tataAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAg                                                                                                                                     >  1:35189/1‑150 (MQ=255)
                        cGTGGTTTCTCTGCAAAAAACTTACGCCAAATTAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTgg                                                                                                                               <  1:339293/150‑1 (MQ=255)
                        cGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCTAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTgg                                                                                                                               <  1:1717117/150‑1 (MQ=255)
                        cGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGTAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTgg                                                                                                                               <  1:1781442/150‑1 (MQ=255)
                        cGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGTAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTgg                                                                                                                               <  1:2513379/150‑1 (MQ=255)
                        cGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTgg                                                                                                                               <  1:1339431/150‑1 (MQ=255)
                             gTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGACAAAGATTTGGCAGAc                                                                                                                          >  2:1802405/1‑150 (MQ=255)
                             gTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGACAAAGATTTGGCAGAc                                                                                                                          >  2:750334/1‑150 (MQ=255)
                             gTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGACAAAGATTTGGCAGAc                                                                                                                          >  2:801296/1‑150 (MQ=255)
                             gTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGGCTGCAGAATTCCCATCGACAAAGATTTGGCAGAc                                                                                                                          >  2:801251/1‑150 (MQ=255)
                                ctctGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAcagc                                                                                                                       <  1:1013547/150‑4 (MQ=255)
                                ctctGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAcagc                                                                                                                       <  1:1155934/150‑4 (MQ=255)
                                 tctGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTg                                                                                                                      >  2:1316800/1‑150 (MQ=255)
                                       aaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGcc                                                                                                                >  2:1915335/1‑150 (MQ=255)
                                         aaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGccca                                                                                                              >  1:2035419/1‑148 (MQ=255)
                                            cTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCATCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATc                                                                                                           <  2:1050687/150‑1 (MQ=255)
                                                  ccAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTccc                                                                                                     <  2:2458803/150‑1 (MQ=255)
                                                   cAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcccc                                                                                                    >  2:2154744/1‑150 (MQ=255)
                                                        gAGGATTTTTTCCATCTTTTTTCAACTTGTTGAAATTCACCAGACAATGTGTGGCTAATTAACACCTTTGGTAATTCCGCAGACACCGTCTGCAGAATTCCAATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGcc                                                                                               <  1:2567907/150‑1 (MQ=255)
                                                           gCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAg                                                                                            >  1:2438418/1‑150 (MQ=255)
                                                           gCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATAAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATAGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAg                                                                                            >  1:2493370/1‑150 (MQ=255)
                                                                     ctctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTAc                                                                                  >  1:1363209/1‑150 (MQ=255)
                                                                     ctctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTAc                                                                                  >  2:2185933/1‑150 (MQ=255)
                                                                      tctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACg                                                                                 >  1:640895/1‑150 (MQ=255)
                                                                      tctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACg                                                                                 >  2:1759308/1‑150 (MQ=255)
                                                                      tctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACg                                                                                 >  2:1759199/1‑150 (MQ=255)
                                                                       ctTTTTTCAACATGGTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGt                                                                                <  1:604820/150‑1 (MQ=255)
                                                                        ttttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATGTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTc                                                                               >  2:356113/1‑150 (MQ=255)
                                                                        ttttttCAACATGTTGAAATTCACCAGACAATGTCAGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTc                                                                               >  2:450592/1‑150 (MQ=255)
                                                                           tttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGt                                                                            >  1:1625569/1‑150 (MQ=255)
                                                                               aaCATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTAc                                                                        <  2:293413/150‑1 (MQ=255)
                                                                               aaCATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTAc                                                                        >  2:1530607/1‑150 (MQ=255)
                                                                               aaCATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTAc                                                                        <  2:418051/150‑1 (MQ=255)
                                                                                    gTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTcc                                                                   >  2:1328233/1‑150 (MQ=255)
                                                                                     ttGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCg                                                                  >  2:2103716/1‑150 (MQ=255)
                                                                                     ttGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCg                                                                  >  1:855767/1‑150 (MQ=255)
                                                                                     ttGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCg                                                                  <  1:2486844/150‑1 (MQ=255)
                                                                                      tGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGt                                                                 >  2:760285/1‑150 (MQ=255)
                                                                                      tGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGt                                                                 >  2:2552699/1‑150 (MQ=255)
                                                                                      gttaaTTCGCCAGACATTGTCTGGTGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGt                                                                 >  2:1561102/4‑150 (MQ=255)
                                                                                       gAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGACGTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTGCCCCTTGCCCAGGTCCACCTCCGGCCGTTTACTTGCCGtt                                                                >  2:566951/1‑150 (MQ=255)
                                                                                       gAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGtt                                                                <  1:459040/150‑1 (MQ=255)
                                                                                        aaaTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTa                                                               <  2:199545/150‑1 (MQ=255)
                                                                                        aaaTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTa                                                               <  2:1532491/150‑1 (MQ=255)
                                                                                           ttCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaaa                                                            <  1:588833/150‑1 (MQ=255)
                                                                                             cACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAAc                                                          <  2:1374824/150‑1 (MQ=255)
                                                                                              aCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACg                                                         <  1:828690/150‑1 (MQ=255)
                                                                                              aCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACg                                                         <  1:14944/150‑1 (MQ=255)
                                                                                               ccAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGc                                                        <  2:546318/150‑1 (MQ=255)
                                                                                                 aGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCCGACGCTGTCTGCCAAATCTTTACCCATGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTg                                                      >  1:1951743/1‑150 (MQ=255)
                                                                                                             gCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTACCCTTGCCCAGGTCCACCTACGTCCGGTTACATTACGTTAAAAAAGCTGACGCCCGCAGCt                                          >  1:2049778/1‑150 (MQ=255)
                                                                                                             gCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCAGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCt                                          >  2:692894/1‑150 (MQ=255)
                                                                                                                 aTTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTa                                      <  1:1853383/150‑1 (MQ=255)
                                                                                                                 aTTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTa                                      <  1:1768073/150‑1 (MQ=255)
                                                                                                                 aTTAACACCATTGGGAATGCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTa                                      <  1:2567909/150‑1 (MQ=255)
                                                                                                                  ttAACACCATTGGGAAATCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGAATTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCCGGTCCACCAACGTCCGTTTACATTCCGTTAAAAACGCTGACGCCCGCAGCTTTTAt                                     >  1:1718963/1‑150 (MQ=255)
                                                                                                                   tAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCCTCGGCAAAGATTTGGCAGACGCTGTCTGCCAAAACATTCCCCTTGCCCAGGTCCCCCTACGGCCGGTTTCTTTACGTTAAAAACGCAGACGCCCGCAGCAGTTATg                                    >  1:2062843/1‑150 (MQ=255)
                                                                                                                   tAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTTGCCGACGCGGTCTGCCAAATATTTCCCCATGCCCCGGTCCACCAACGTCCCTTTACATTCCGGTAAAAACGCTGACGCCCGCAGCGTTTATg                                    >  1:1209239/1‑150 (MQ=255)
                                                                                                                        ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCTGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa                               <  2:386261/150‑1 (MQ=255)
                                                                                                                        ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa                               <  1:51312/150‑1 (MQ=255)
                                                                                                                              ggAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACg                         <  1:1530607/150‑1 (MQ=255)
                                                                                                                                  ttCTGCAGACGGTGTCTGCGGAATTCCCATCGGCAAAGATTTGGCAGACGCGGTCTGCCAAATATTTCCCCGTGCCCAGGTCCACCGAAGTCCCTTTAAATTCCGTTTAAAACGCAGAAGCCCCCAGCTTTTATGGAAAATAGAAGcaac                     >  1:1065452/1‑147 (MQ=255)
                                                                                                                                   tCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAATATTTGGCAGACGCTGTCTGCCAAATCGTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAAAGCGGACGCCCGCAGCTTTTATGAAAAAGAGACGCTACg                    >  2:512654/1‑150 (MQ=255)
                                                                                                                                   tCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCg                    >  2:722585/1‑150 (MQ=255)
                                                                                                                                   tCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATATTTCCCCTTGCCCAGGTCCACCAACGTCCGTTTAAATTACGTTAAAAACGCTGACGCCCGCAGCTTTTATGTAAAAGAGACGCTCCg                    >  1:2058016/1‑150 (MQ=255)
                                                                                                                                    ccGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTATGCCAAATCTTTCCCCTTGCCCAGGTCCAACTACGACCGTTTACTTTACGTTAAAAACGCTGACGCCCGCAGCTAAAATGAAAAAGAGACGCTCCGc                   >  2:645483/1‑150 (MQ=255)
                                                                                                                                     cGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCt                  >  2:795620/1‑150 (MQ=255)
                                                                                                                                     cGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCt                  >  2:1238397/1‑150 (MQ=255)
                                                                                                                                     cGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGAAGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCt                  >  2:1238394/1‑150 (MQ=255)
                                                                                                                                      gCAGACACCGTCTGCAGAATTCCCATCGGCAATGATTTTGCAGAAGCTGTCTGCCAAATCTTTACCCATGCCCAGGTCCACCGACGTCCGTTTTCTTTGCGTTAAAAACGCCGACGCCCCCCGCATTTTTGAAAAAGAGACGCTCCGcgg                 >  1:1007625/1‑148 (MQ=255)
                                                                                                                                         gACACCGTCTGCAGAATTCCCATCGGCACAGCTTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTgg              <  2:511991/150‑1 (MQ=255)
                                                                                                                                          acggtgTCTGCGGAATTCCCATCGGCAAAGATTTTGCAGAAGCTGTCTGCCAAATCTTTCCCCATTCCCAGGTCCACCAACCTCCGGTTAAATTCCCTTAAAAAAGCTGACGCCCCCACCATTTATGAAAAAGAGAAGCACCGCGGGGGc             >  1:333244/6‑150 (MQ=255)
                                                                                                                                          acacCGTCTGCAGAATTCCCATCGGCAAAGATTTTGCAGAAGCGGTCTGCCAAATATTTACCCATTCCCAGGTCCACCAACGTCCGTTTACATTGCGTTAAAAAAGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGc             >  1:134413/1‑150 (MQ=255)
                                                                                                                                           cacCGTCTGCAGAATTCCCATCGGCAAAGATTTGTCATACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCTTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCt            <  1:8058/150‑1 (MQ=255)
                                                                                                                                                tCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCGTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCt       <  1:575674/150‑1 (MQ=255)
                                                                                                                                                  tGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGt     >  2:826138/1‑150 (MQ=255)
                                                                                                                                                  tGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTAGGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGt     >  2:2291327/1‑150 (MQ=255)
                                                                                                                                                   gCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGtt    <  1:1328233/150‑1 (MQ=255)
                                                                                                                                                    cAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTc   <  1:553964/150‑1 (MQ=255)
                                                                                                                                                    cAGAATTCCCATCGGCAAAGATTTGCCCGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGGTTACATTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAACAAGAGACGCTCCGCTGTGGCTGGTCTGTTc   >  2:15275/1‑150 (MQ=255)
                                                                                                                                                     aGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCg  >  2:1896034/1‑150 (MQ=255)
                                                                                                                                                     aGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCg  >  2:1895987/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCG  >  NC_000913/3368040‑3368338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: