Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,489,998 A→G 5.5% P61P (CCT→CCC argD ← bifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,489,9980AG 5.5% 497.4 / 13.9 146P61P (CCT→CCCargDbifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (78/59);  new base G (4/4);  total (82/64)
Fisher's exact test for biased strand distribution p-value = 7.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 149 total aligned reads displayed.

GCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGT  >  NC_000913/3489849‑3490147
                                                                                                                                                     |                                                                                                                                                     
gCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCa                                                                                                                                                       <  1:738894/150‑1 (MQ=255)
       tGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGc                                                                                                                                                <  2:1683198/150‑1 (MQ=255)
       tGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGATTTTAACGCGTTCACCAGCGCAGGATGGc                                                                                                                                                <  2:2507193/150‑1 (MQ=255)
           ggAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATg                                                                                                                                            >  1:964639/1‑150 (MQ=255)
             aGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGc                                                                                                                                          <  1:2185470/150‑1 (MQ=255)
             aGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGc                                                                                                                                          <  2:2249475/150‑1 (MQ=255)
             aGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGc                                                                                                                                          <  1:1836305/150‑1 (MQ=255)
               ttCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGccc                                                                                                                                        >  1:907956/1‑150 (MQ=255)
                 cATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCaa                                                                                                                                      >  1:2470352/1‑150 (MQ=255)
                  aTAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAAc                                                                                                                                     >  1:395984/1‑150 (MQ=255)
                   tAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACg                                                                                                                                    >  2:1929138/1‑150 (MQ=255)
                    aaaCACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGc                                                                                                                                   >  1:1659277/1‑150 (MQ=255)
                     aaCACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCGGGATGGCAATGGCCCAACGcc                                                                                                                                  >  2:1145025/1‑150 (MQ=255)
                     aaCACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCGGGATGGCAATGGCCCAACGcc                                                                                                                                  >  2:2424170/1‑150 (MQ=255)
                     aaCACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCGGGATGGCAATGGCCCAACGcc                                                                                                                                  >  2:2424268/1‑150 (MQ=255)
                            cgcgTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTg                                                                                                                           <  2:2200466/150‑1 (MQ=255)
                            cgcgTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTg                                                                                                                           <  2:168025/150‑1 (MQ=255)
                            cgcgTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTg                                                                                                                           <  2:609476/150‑1 (MQ=255)
                            cgcgTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTg                                                                                                                           >  1:290169/1‑150 (MQ=255)
                            cgcgTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTg                                                                                                                           <  2:960661/150‑1 (MQ=255)
                              cgTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCa                                                                                                                         <  2:1208207/150‑1 (MQ=255)
                              cgTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCa                                                                                                                         <  2:2462070/150‑1 (MQ=255)
                               gTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCaa                                                                                                                        <  2:2216774/150‑1 (MQ=255)
                               gTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCaa                                                                                                                        >  1:1304660/1‑150 (MQ=255)
                                ttCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAAt                                                                                                                       >  2:1126809/1‑150 (MQ=255)
                                ttCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAAt                                                                                                                       >  1:774389/1‑150 (MQ=255)
                                          gTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCcgcg                                                                                                             >  1:1559752/1‑150 (MQ=255)
                                            tGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGaa                                                                                                           >  1:1120230/1‑150 (MQ=255)
                                               cTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATc                                                                                                        >  1:417968/1‑150 (MQ=255)
                                               cTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATc                                                                                                        >  2:2375437/1‑150 (MQ=255)
                                               cTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATc                                                                                                        <  1:1379843/150‑1 (MQ=255)
                                               cTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATc                                                                                                        >  1:927497/1‑150 (MQ=255)
                                                tCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCg                                                                                                       >  1:2476294/1‑150 (MQ=255)
                                                tCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCg                                                                                                       >  1:1342871/1‑150 (MQ=255)
                                                tCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCg                                                                                                       >  1:606525/1‑150 (MQ=255)
                                                 cAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGa                                                                                                      <  2:505936/150‑1 (MQ=255)
                                                 cAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGa                                                                                                      <  2:1806932/150‑1 (MQ=255)
                                                   aTCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACa                                                                                                    <  2:757561/150‑1 (MQ=255)
                                                    tCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACAt                                                                                                   >  1:2437337/1‑150 (MQ=255)
                                                    tCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACAt                                                                                                   >  2:913845/1‑150 (MQ=255)
                                                    tCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACAt                                                                                                   >  1:2437334/1‑150 (MQ=255)
                                                              cccAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTccttgcc                                                                                         >  1:365289/1‑150 (MQ=255)
                                                                   gACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCt                                                                                    <  2:1017496/150‑1 (MQ=255)
                                                                   gACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCt                                                                                    >  2:1203021/1‑150 (MQ=255)
                                                                      gCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGAt                                                                                 >  1:1800770/1‑150 (MQ=255)
                                                                          cgcCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCa                                                                             <  1:29405/150‑1 (MQ=255)
                                                                          cgcCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCa                                                                             <  1:561110/150‑1 (MQ=255)
                                                                          cgcCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCa                                                                             <  2:1496763/150‑1 (MQ=255)
                                                                          cgcCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCa                                                                             <  2:954938/150‑1 (MQ=255)
                                                                                     tGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGc                                                                  >  2:665376/1‑150 (MQ=255)
                                                                                     tGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGc                                                                  >  1:1603876/1‑150 (MQ=255)
                                                                                     tGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGc                                                                  >  2:1920127/1‑150 (MQ=255)
                                                                                       gTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATc                                                                                <  2:109722/134‑1 (MQ=255)
                                                                                       gTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATc                                                                                >  1:109722/1‑134 (MQ=255)
                                                                                          aaaaCGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGa                                                             >  1:2382403/1‑150 (MQ=255)
                                                                                          aaaaCGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGa                                                             >  2:841823/1‑150 (MQ=255)
                                                                                          aaaaCGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGa                                                             >  2:50381/1‑150 (MQ=255)
                                                                                          aaaaCGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGa                                                             >  1:1375005/1‑150 (MQ=255)
                                                                                           aaaCGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCTCGAAATCGACTTACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGAc                                                            <  2:1004925/150‑1 (MQ=255)
                                                                                           aaaCGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGAc                                                            <  2:816351/150‑1 (MQ=255)
                                                                                           aaaCGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGAc                                                            <  2:816380/150‑1 (MQ=255)
                                                                                           aaaCGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGAc                                                            <  2:1622228/150‑1 (MQ=255)
                                                                                            aaCGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGAcc                                                           >  2:855764/1‑150 (MQ=255)
                                                                                                    tGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCGGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCg                                                   <  1:2424170/150‑1 (MQ=255)
                                                                                                    tGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCGGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCg                                                   <  1:1145025/150‑1 (MQ=255)
                                                                                                    tGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCAAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTGTTACCg                                                   >  1:17417/1‑150 (MQ=255)
                                                                                                     gATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCgg                                                  <  1:2080897/150‑1 (MQ=255)
                                                                                                       tatGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGaa                                                >  2:2170691/1‑150 (MQ=255)
                                                                                                       tatGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGaa                                                <  2:774389/150‑1 (MQ=255)
                                                                                                         tGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATa                                              <  2:203794/150‑1 (MQ=255)
                                                                                                           ccACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATaaa                                            <  2:448757/150‑1 (MQ=255)
                                                                                                           ccACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATaaa                                            <  2:1091283/150‑1 (MQ=255)
                                                                                                            cacaGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAAc                                           >  1:993239/1‑150 (MQ=255)
                                                                                                             acaGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAAct                                          >  2:1427031/1‑150 (MQ=255)
                                                                                                             acaGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAAct                                          >  2:1422998/1‑150 (MQ=255)
                                                                                                             acaGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAAct                                          >  2:1566075/1‑150 (MQ=255)
                                                                                                               agagTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAActct                                        >  2:231564/1‑150 (MQ=255)
                                                                                                               agagTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAActct                                        >  2:1831029/1‑150 (MQ=255)
                                                                                                                   tttCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCg                                    >  1:504861/1‑150 (MQ=255)
                                                                                                                            gggTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATaaa                           >  1:139836/1‑150 (MQ=255)
                                                                                                                              gTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATc                         >  1:1483691/1‑150 (MQ=255)
                                                                                                                              gTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATc                         >  1:789061/1‑150 (MQ=255)
                                                                                                                                   tAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAg                    <  1:307892/150‑1 (MQ=255)
                                                                                                                                   tAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAg                    <  2:1659277/150‑1 (MQ=255)
                                                                                                                                    aaCGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAgg                   >  2:912283/1‑150 (MQ=255)
                                                                                                                                     aCGCGTTCACCAGCGCTGGTTGGCAAGGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTTATCCCATATTCGGCTGCCCTGACCTTTTACCGTAATAAACTCTGCCGGAGCATAAATCGGCAGGa                  <  1:2217447/150‑1 (MQ=255)
                                                                                                                                     aCGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGa                  <  2:2392716/150‑1 (MQ=255)
                                                                                                                                     aCGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGa                  <  2:1375005/150‑1 (MQ=255)
                                                                                                                                       gcgTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGACTAAACTCTGCCGGAGCATAAATCGGCAGGATc                >  1:2114549/1‑150 (MQ=255)
                                                                                                                                             aCCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCa          >  1:52002/1‑150 (MQ=255)
                                                                                                                                               cAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATc        >  2:1321843/1‑150 (MQ=255)
                                                                                                                                               cAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATAGGCAGGATCACTTCATc        >  2:540163/1‑150 (MQ=255)
                                                                                                                                                aGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCg       >  2:305811/1‑150 (MQ=255)
                                                                                                                                                aGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCg       >  2:778514/1‑150 (MQ=255)
                                                                                                                                                aGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCg       >  2:2137082/1‑150 (MQ=255)
                                                                                                                                                aGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCg       >  2:2105931/1‑150 (MQ=255)
                                                                                                                                                aGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCg       >  2:1108826/1‑150 (MQ=255)
                                                                                                                                                  cgcAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGaa     <  1:1270729/150‑1 (MQ=255)
                                                                                                                                                     aGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGACTAAACTCTGCCGGAGCATAAATCGGCCGGATCCCTTCCTCGAAAGt  >  1:806261/1‑150 (MQ=255)
                                                                                                                                                     aGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGt  >  1:1278285/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGT  >  NC_000913/3489849‑3490147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: