Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,657,603 T→A 5.7% intergenic (+36/+383) hdeD → / ← arrS acid‑resistance membrane protein/antisense sRNA ArrS, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,657,6030TA 5.7% 447.2 / 11.6 123intergenic (+36/+383)hdeD/arrSacid‑resistance membrane protein/antisense sRNA ArrS, function unknown
Reads supporting (aligned to +/- strand):  ref base T (55/61);  new base A (5/2);  total (60/63)
Fisher's exact test for biased strand distribution p-value = 2.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 127 total aligned reads displayed.

GGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCATTTCAA  >  NC_000913/3657454‑3657752
                                                                                                                                                     |                                                                                                                                                     
ggTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAt                                                                                                                                                       <  1:1163105/150‑1 (MQ=255)
ggTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAt                                                                                                                                                       <  1:425912/150‑1 (MQ=255)
    cAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGa                                                                                                                                                   <  1:234250/150‑1 (MQ=255)
        gCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTAt                                                                                                                                               <  1:55142/150‑1 (MQ=255)
        gCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTAt                                                                                                                                               <  2:2475079/150‑1 (MQ=255)
           gATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGAt                                                                                                                                            >  1:2459303/1‑150 (MQ=255)
            aTGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATc                                                                                                                                           <  1:1349286/150‑1 (MQ=255)
               gtgtCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGa                                                                                                                                        <  2:1798152/150‑1 (MQ=255)
               gtgtCTGGAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGa                                                                                                                                        <  2:1798259/150‑1 (MQ=255)
                tgtCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGAt                                                                                                                                       >  2:154734/1‑150 (MQ=255)
                    tGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAAt                                                                                                                                   >  2:1260900/1‑150 (MQ=255)
                     gTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATa                                                                                                                                  >  2:870708/1‑150 (MQ=255)
                     gTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGATCCgggtgaca                                                                                                                                  <  2:1873740/150‑8 (MQ=39)
                     gTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGATCCgggtgaca                                                                                                                                  <  2:298029/150‑8 (MQ=39)
                        aCGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATActc                                                                                                                               >  2:394413/1‑150 (MQ=255)
                          gCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATActctc                                                                                                                             >  2:944628/1‑150 (MQ=255)
                          gCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATActctc                                                                                                                             >  2:659003/1‑150 (MQ=255)
                            tGGTTTCCACACTGGTGGGCATTGAACTGATATTTAGCGCCGCCAGCCTGCTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCTGATCATAGTCACTGTATGTGACTATGATCCGATTAATACtctctc                                                                                                                           <  2:1386102/150‑1 (MQ=255)
                              gTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCg                                                                                                                         >  2:1060989/1‑150 (MQ=255)
                                   cacaCTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACg                                                                                                                    <  1:2115678/150‑1 (MQ=255)
                                    acacTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGc                                                                                                                   >  1:354738/1‑150 (MQ=255)
                                     cacTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCa                                                                                                                  <  1:2142109/150‑1 (MQ=255)
                                     cacTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCa                                                                                                                  <  1:1771368/150‑1 (MQ=255)
                                     cacTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCa                                                                                                                  <  1:1771360/150‑1 (MQ=255)
                                     cacTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCa                                                                                                                  <  2:1922745/150‑1 (MQ=255)
                                      acTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAg                                                                                                                 >  1:1686837/1‑150 (MQ=255)
                                          gtggGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgtt                                                                                                             >  2:450950/1‑150 (MQ=255)
                                            gggAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgttgt                                                                                                           >  1:2316934/1‑150 (MQ=255)
                                            gggAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgttgt                                                                                                           >  2:472193/1‑150 (MQ=255)
                                            gggAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgttgt                                                                                                           >  1:1074686/1‑150 (MQ=255)
                                             ggAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTa                                                                                                          <  2:2317434/150‑1 (MQ=255)
                                             ggAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTa                                                                                                          <  2:742373/150‑1 (MQ=255)
                                               aaTTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGa                                                                                                        >  1:117654/1‑150 (MQ=255)
                                                     aCTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAAtt                                                                                                  >  2:1155979/1‑150 (MQ=255)
                                                       tGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTgc                                                                                                >  2:210790/1‑150 (MQ=255)
                                                        gATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTgcg                                                                                               <  1:1300728/150‑1 (MQ=255)
                                                         atatTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATATTTACCCCGGTTGTCACCAGGATCATAGTCACATGATGTGACTATGATCCGATAAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTgcgc                                                                                              >  2:1562754/1‑150 (MQ=255)
                                                         atatTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTgcgc                                                                                              >  2:1562674/1‑150 (MQ=255)
                                                         atatTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTgcgc                                                                                              >  2:378180/1‑150 (MQ=255)
                                                         atatTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTgagc                                                                                              >  2:378186/1‑150 (MQ=255)
                                                          tatTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCa                                                                                             >  1:2175694/1‑150 (MQ=255)
                                                             ttAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTa                                                                                          >  2:1083621/1‑150 (MQ=255)
                                                               aGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATc                                                                                        <  2:925987/150‑1 (MQ=255)
                                                                gcgcCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCa                                                                                       <  2:1849400/150‑1 (MQ=255)
                                                                gcgcCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCa                                                                                       <  1:681721/150‑1 (MQ=255)
                                                                gcgcCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCa                                                                                       <  2:299982/150‑1 (MQ=255)
                                                                   ccgccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCCATTGCGCACTATCATTg                                                                                    >  1:2285418/1‑150 (MQ=255)
                                                                    cgccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGa                                                                                   <  2:212228/150‑1 (MQ=255)
                                                                     gccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGaa                                                                                  <  1:1665218/150‑1 (MQ=255)
                                                                      ccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGaaa                                                                                 <  1:129938/150‑1 (MQ=255)
                                                                      ccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGaaa                                                                                 >  1:2459067/1‑150 (MQ=255)
                                                                      ccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGaaa                                                                                 >  2:1478057/1‑150 (MQ=255)
                                                                      ccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGaaa                                                                                 <  1:1835937/150‑1 (MQ=255)
                                                                          ccTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAaataat                                                                             <  1:688397/150‑1 (MQ=255)
                                                                          ccTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAaataat                                                                             <  1:688347/150‑1 (MQ=255)
                                                                            tGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTa                                                                           <  2:1783470/150‑1 (MQ=255)
                                                                            tGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTa                                                                           >  2:2077037/1‑150 (MQ=255)
                                                                             gTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTAc                                                                          <  2:386553/150‑1 (MQ=255)
                                                                             gTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTAc                                                                          <  2:386746/150‑1 (MQ=255)
                                                                               tCAGCTTCTCCAGTTTTTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCt                                                                        <  2:23285/150‑1 (MQ=255)
                                                                                cAGCTTCGCCATTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTg                                                                       <  1:995310/150‑1 (MQ=255)
                                                                                cAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTg                                                                       <  1:995341/150‑1 (MQ=255)
                                                                                   cTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTa                                                                    >  1:912636/1‑150 (MQ=255)
                                                                                        ccAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGat                                                               <  2:1009765/150‑1 (MQ=255)
                                                                                        ccAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGat                                                               <  2:2311731/150‑1 (MQ=255)
                                                                                         cAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGatt                                                              >  1:21454/1‑150 (MQ=255)
                                                                                                ttCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCaa                                                       >  2:431619/1‑150 (MQ=255)
                                                                                                 tCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAAc                                                      <  2:813198/150‑1 (MQ=255)
                                                                                                 tCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAAc                                                      <  2:1901006/150‑1 (MQ=255)
                                                                                                 tCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAAc                                                      <  2:2418587/150‑1 (MQ=255)
                                                                                                 tCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAAc                                                      <  2:879161/150‑1 (MQ=255)
                                                                                                       agcagcAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTg                                                >  1:979507/1‑150 (MQ=255)
                                                                                                        gcagcaATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGa                                               <  1:1162313/150‑1 (MQ=255)
                                                                                                         cagcaATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGaa                                              >  2:2203698/1‑150 (MQ=255)
                                                                                                         cagcaATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGaa                                              >  2:1559513/1‑150 (MQ=255)
                                                                                                              ataatTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCa                                         <  1:1607027/150‑1 (MQ=255)
                                                                                                                aatTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATc                                       >  2:947514/1‑150 (MQ=255)
                                                                                                                        cGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAAttttt                               >  2:1391202/1‑150 (MQ=255)
                                                                                                                        cGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTGGTAGATCAAGTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGACTTTCATCTAAttttt                               >  2:1325142/1‑150 (MQ=255)
                                                                                                                          gTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCTATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCTCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAAttttttt                             <  2:2531281/150‑1 (MQ=255)
                                                                                                                            tGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTTCTAGTGATTATTTCAACCTACTGAATGTCATCTAATTTTTTTCa                           >  1:2192160/1‑150 (MQ=255)
                                                                                                                                 cccGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTa                      <  1:1994400/150‑1 (MQ=255)
                                                                                                                                 cccGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTa                      <  1:1994553/150‑1 (MQ=255)
                                                                                                                                 cccGGATCATAGTCACTTGATGTGACTATGATCCGAATAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTa                      <  2:2370868/150‑1 (MQ=255)
                                                                                                                                    ggATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATgg                   <  2:628663/150‑1 (MQ=255)
                                                                                                                                     gATCATAGTCACATCAAGTGACTATGATCCGGTTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGc                  >  1:744650/1‑150 (MQ=255)
                                                                                                                                     gATCATAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGc                  >  1:599896/1‑150 (MQ=255)
                                                                                                                                         aTAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAAt              >  1:314955/1‑150 (MQ=255)
                                                                                                                                          tAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAAtt             >  1:2523301/1‑150 (MQ=255)
                                                                                                                                          tAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAAtt             >  2:1515886/1‑150 (MQ=255)
                                                                                                                                           aGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTa            >  1:1329451/1‑150 (MQ=255)
                                                                                                                                            gTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAg           <  1:2420016/150‑1 (MQ=255)
                                                                                                                                             tCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGc          <  1:1662485/150‑1 (MQ=255)
                                                                                                                                             tCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGc          <  2:2316934/150‑1 (MQ=255)
                                                                                                                                               aCTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCa        >  1:2023079/1‑150 (MQ=255)
                                                                                                                                                cTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCAt       >  2:79163/1‑150 (MQ=255)
                                                                                                                                                cTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCAt       >  1:96622/1‑150 (MQ=255)
                                                                                                                                                cTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCAt       >  2:272560/1‑150 (MQ=255)
                                                                                                                                                    aTGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCATTTCa   >  2:1241518/1‑150 (MQ=255)
                                                                                                                                                     tgtgACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCATTTCaa  <  1:870708/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCATTTCAA  >  NC_000913/3657454‑3657752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: