Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,162,152 G→C 5.5% intergenic (+22/+18) yijD → / ← trmA DUF1422 family inner membrane protein/tRNA m(5)U54 methyltransferase, SAM‑dependent; tmRNA m(5)U341 methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,162,1520GC 5.5% 739.4 / 27.5 199intergenic (+22/+18)yijD/trmADUF1422 family inner membrane protein/tRNA m(5)U54 methyltransferase, SAM‑dependent; tmRNA m(5)U341 methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (100/88);  new base C (3/8);  total (103/96)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 206 total aligned reads displayed.

CAGCACGGTAGTTCGTGCGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAGATTCTTGCATAACGTTTCCGGGTTACA  >  NC_000913/4162004‑4162301
                                                                                                                                                    |                                                                                                                                                     
cAGCACGGTAGTTCGTGCGGACTATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGgc                                                                                                                                                      >  2:202346/1‑150 (MQ=255)
cAGCACGGTAGTTCGTGCGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGgc                                                                                                                                                      <  1:224142/150‑1 (MQ=255)
cAGCACGGTAGTTCGTGCGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGgc                                                                                                                                                      <  2:1374715/150‑1 (MQ=255)
      ggTAGTTCGTGCGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATcc                                                                                                                                                <  1:2292737/150‑1 (MQ=255)
        tAGTTCGTGCGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCgg                                                                                                                                              <  2:663905/150‑1 (MQ=255)
        tAGTTCGTGCGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCGCCAGCATCCgg                                                                                                                                              <  2:1583264/150‑1 (MQ=255)
        tAGTTCGTGCGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCGCCAGCATCCgg                                                                                                                                              <  2:1010371/150‑1 (MQ=255)
        tAGTTCGTGCGGAATATCCGGATATCGGCTCTAACTACTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCgg                                                                                                                                              <  2:1905950/150‑1 (MQ=255)
        gaGTTCGTGCGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCGCCAGCATCCgg                                                                                                                                              <  2:131663/149‑1 (MQ=255)
            tCGTGCGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATg                                                                                                                                          <  1:1611159/150‑1 (MQ=255)
                 cGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGttt                                                                                                                                     <  1:1284695/150‑1 (MQ=255)
                   gAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTa                                                                                                                                   <  1:465925/150‑1 (MQ=255)
                   gAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTa                                                                                                                                   <  2:533397/150‑1 (MQ=255)
                   gAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTa                                                                                                                                   >  1:2450102/1‑150 (MQ=255)
                    aaTATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTAc                                                                                                                                  >  1:1787189/1‑150 (MQ=255)
                     atatCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACt                                                                                                                                 >  2:1857936/1‑150 (MQ=255)
                       atCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTc                                                                                                                               <  1:1621444/150‑1 (MQ=255)
                        tCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTcg                                                                                                                              >  2:1161878/1‑150 (MQ=255)
                        tCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTcg                                                                                                                              >  1:981950/1‑150 (MQ=255)
                        tCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTcg                                                                                                                              >  2:1291167/1‑150 (MQ=255)
                         ccGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTcgc                                                                                                                             <  2:519430/150‑1 (MQ=255)
                          cGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTcgcg                                                                                                                            <  1:1538400/150‑1 (MQ=255)
                          cGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTcgcg                                                                                                                            <  1:343019/150‑1 (MQ=255)
                           ggATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCgg                                                                                                                           >  1:1743448/1‑150 (MQ=255)
                              tatCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCa                                                                                                                        <  2:2003767/150‑1 (MQ=255)
                                    ctctAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATa                                                                                                                  >  1:1528919/1‑150 (MQ=255)
                                    ctctAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATa                                                                                                                  >  2:1922352/1‑150 (MQ=255)
                                     tctAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATAc                                                                                                                 >  1:965710/1‑150 (MQ=255)
                                       tAACTTCGTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATAcgc                                                                                                               <  2:955691/150‑1 (MQ=255)
                                           ttcttcCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCa                                                                                                           <  2:863730/150‑1 (MQ=255)
                                              ttcCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTc                                                                                                        >  2:370484/1‑150 (MQ=255)
                                                cccGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCa                                                                                                      <  2:450882/150‑1 (MQ=255)
                                                cccGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCa                                                                                                      <  1:1182942/150‑1 (MQ=255)
                                                cccGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCa                                                                                                      >  2:2514770/1‑150 (MQ=255)
                                                 ccGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCat                                                                                                     >  1:444100/1‑150 (MQ=255)
                                                 ccGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCat                                                                                                     >  1:924995/1‑150 (MQ=255)
                                                  cGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCata                                                                                                    >  1:1855627/1‑150 (MQ=255)
                                                        tACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGc                                                                                              >  1:1866475/1‑150 (MQ=255)
                                                        tACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGc                                                                                              >  2:1663072/1‑150 (MQ=255)
                                                             tcCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTa                                                                                         >  1:1453391/1‑150 (MQ=255)
                                                             tcCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTa                                                                                         >  2:555661/1‑150 (MQ=255)
                                                             tcCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTAGTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTa                                                                                         >  2:2098834/1‑150 (MQ=255)
                                                               cGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAgg                                                                                       >  2:2294268/1‑150 (MQ=255)
                                                                gTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAggg                                                                                      <  2:591345/150‑1 (MQ=255)
                                                                gTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAggg                                                                                      >  1:1247180/1‑150 (MQ=255)
                                                                 tCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAgggg                                                                                     >  2:430719/1‑150 (MQ=255)
                                                                    ttATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAAc                                                                                  <  1:419312/150‑1 (MQ=255)
                                                                     tATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACt                                                                                 >  1:2214415/1‑150 (MQ=255)
                                                                        ggTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGAt                                                                              <  2:47772/150‑1 (MQ=255)
                                                                        ggTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGAt                                                                              <  2:260851/150‑1 (MQ=255)
                                                                          tgtTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGAtga                                                                            <  2:1062286/150‑3 (MQ=255)
                                                                            ttCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCaaa                                                                          <  1:2433941/150‑1 (MQ=255)
                                                                            ttCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCaaa                                                                          <  1:370484/150‑1 (MQ=255)
                                                                             tCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCAGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAAc                                                                         >  1:1712923/1‑150 (MQ=255)
                                                                              cTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACa                                                                        >  1:1023634/1‑150 (MQ=255)
                                                                               tGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACag                                                                       >  2:2341090/1‑150 (MQ=255)
                                                                               tGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACag                                                                       >  2:478662/1‑150 (MQ=255)
                                                                               tGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACag                                                                       >  2:711627/1‑150 (MQ=255)
                                                                               tGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACag                                                                       >  1:1760562/1‑150 (MQ=255)
                                                                                  aTTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGc                                                                    <  1:748157/150‑1 (MQ=255)
                                                                                     ggCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAg                                                                 <  2:1338446/150‑1 (MQ=255)
                                                                                      gcgcGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCATGTAGGGGAACTGATCAAACAGAGCCAGa                                                                <  1:2559179/150‑1 (MQ=255)
                                                                                             gATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGa                                                         <  2:1108258/150‑1 (MQ=255)
                                                                                             gATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGa                                                         <  1:1117862/150‑1 (MQ=255)
                                                                                             gATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGa                                                         <  1:1283627/150‑1 (MQ=255)
                                                                                              aTGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGAc                                                        <  1:1642903/150‑1 (MQ=255)
                                                                                               tGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGAcc                                                       >  2:2027145/1‑150 (MQ=255)
                                                                                               tGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGAcc                                                       >  2:2066516/1‑150 (MQ=255)
                                                                                                gCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCt                                                      >  2:2388717/1‑150 (MQ=255)
                                                                                                   tAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTtgt                                                   >  2:918123/1‑150 (MQ=255)
                                                                                                   tAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTtgt                                                   >  2:1199892/1‑150 (MQ=255)
                                                                                                       cGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGt                                               >  1:1043208/1‑150 (MQ=255)
                                                                                                       cGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGt                                               >  1:2110891/1‑150 (MQ=255)
                                                                                                        gTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTc                                              <  1:1922352/150‑1 (MQ=255)
                                                                                                        gTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTc                                              >  2:4163/1‑150 (MQ=255)
                                                                                                         tAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCt                                             <  2:843893/150‑1 (MQ=255)
                                                                                                         tAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCt                                             >  2:1011571/1‑150 (MQ=255)
                                                                                                           aGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTgg                                           <  1:2504377/150‑1 (MQ=255)
                                                                                                           aGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTgg                                           <  2:1456394/150‑1 (MQ=255)
                                                                                                            gCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGc                                          <  1:1228835/150‑1 (MQ=255)
                                                                                                              aGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCtt                                        >  2:1148879/1‑150 (MQ=255)
                                                                                                              aGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCtt                                        >  2:1688812/1‑150 (MQ=255)
                                                                                                              aGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCAAATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCATGTGCGTCTGGCtt                                        >  1:603637/1‑150 (MQ=255)
                                                                                                                gAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGCAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTaa                                      <  1:1899509/150‑1 (MQ=255)
                                                                                                                   gTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGt                                   <  2:1290979/150‑1 (MQ=255)
                                                                                                                   gTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGt                                   <  2:2445342/150‑1 (MQ=255)
                                                                                                                     tGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGttt                                 <  1:140567/150‑1 (MQ=255)
                                                                                                                        tGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCa                              >  2:2295364/1‑150 (MQ=255)
                                                                                                                                    attatGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGTGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAGATTCTTGCATa                  <  2:1442/150‑1 (MQ=255)
                                                                                                                                    attatGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAGATTCTTGCATa                  <  1:586746/150‑1 (MQ=255)
                                                                                                                                       atGCCGGATGCGCCAGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAGATTCTTGCATAACg               >  1:2577320/1‑150 (MQ=255)
                                                                                                                                       atGCCGGATGCGCCAGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAGATTCTTGCATAACg               >  1:1953616/1‑150 (MQ=255)
                                                                                                                                          ccGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAGATTCTTGCATAACGttt            >  2:2273843/1‑150 (MQ=255)
                                                                                                                                             gATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAGATTCTTGCATAACGTTTCCg         >  2:2451821/1‑150 (MQ=255)
                                                                                                                                             gATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAGATTCTTGCATAACGTTTCCg         >  2:2238200/1‑150 (MQ=255)
                                                                                                                                             gATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAGATTCTTGCATAACGTTTCCg         >  2:2451732/1‑150 (MQ=255)
                                                                                                                                               tGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAGATTCTTGCATAACGTTTCCggg       >  1:910403/1‑150 (MQ=255)
                                                                                                                                                 cTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAGATTCTTGCATAACGTTTCCGGGtt     >  2:1747736/1‑150 (MQ=255)
                                                                                                                                                  tGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAGATTCTTGCATAACGTTTCCGGGTTa    <  2:1228520/150‑1 (MQ=255)
                                                                                                                                                    gcgcATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAGATTCTTGCATAACGTTTCCGGGTTACa  >  1:1590392/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAGCACGGTAGTTCGTGCGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAGATTCTTGCATAACGTTTCCGGGTTACA  >  NC_000913/4162004‑4162301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: