Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,460,483 T→A 6.2% intergenic (‑119/+39) nrdG ← / ← nrdD anaerobic ribonucleotide reductase activating protein/anaerobic ribonucleoside‑triphosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,460,4830TA 6.2% 809.8 / 26.7 222intergenic (‑119/+39)nrdG/nrdDanaerobic ribonucleotide reductase activating protein/anaerobic ribonucleoside‑triphosphate reductase
Reads supporting (aligned to +/- strand):  ref base T (129/79);  new base A (11/3);  total (140/82)
Fisher's exact test for biased strand distribution p-value = 2.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 225 total aligned reads displayed.

TGTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGCACACGCGG  >  NC_000913/4460334‑4460630
                                                                                                                                                     |                                                                                                                                                   
tGTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGtt                                                                                                                                                     <  1:166706/150‑1 (MQ=255)
 gTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGttt                                                                                                                                                    <  2:1785252/150‑1 (MQ=255)
  tCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTa                                                                                                                                                   >  2:162600/1‑150 (MQ=255)
    gACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTAcg                                                                                                                                                 >  1:479858/1‑150 (MQ=255)
    gACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTAcg                                                                                                                                                 >  1:1900234/1‑150 (MQ=255)
      cAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                               <  1:408180/150‑1 (MQ=255)
      cAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                               <  1:2217083/150‑1 (MQ=255)
      cAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                               >  1:722726/1‑150 (MQ=255)
           tAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                                                                                          >  1:2541847/1‑150 (MQ=255)
            aGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                         <  2:2563513/150‑1 (MQ=255)
              tACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                       >  1:371331/1‑150 (MQ=255)
                     gatAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGc                                                                                                                                >  2:783437/1‑150 (MQ=255)
                     gatAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGc                                                                                                                                >  2:755603/1‑150 (MQ=255)
                     gatAATTCATTATTTCGCCTGGCTTATCAGACCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGGTTACGCCGCATCCGGCATTTGc                                                                                                                                >  2:1587338/1‑150 (MQ=255)
                        aaTTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTa                                                                                                                             <  2:2057672/150‑1 (MQ=255)
                         aTTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAg                                                                                                                            >  1:524993/1‑150 (MQ=255)
                         aTTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAg                                                                                                                            >  2:366001/1‑150 (MQ=255)
                           tCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCt                                                                                                                          >  2:563677/1‑150 (MQ=255)
                           tCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCt                                                                                                                          >  1:302315/1‑150 (MQ=255)
                           tCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCt                                                                                                                          >  1:714649/1‑150 (MQ=255)
                           tCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCt                                                                                                                          >  2:1110033/1‑150 (MQ=255)
                           tCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCt                                                                                                                          >  1:943226/1‑150 (MQ=255)
                           tCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCt                                                                                                                          >  2:2376286/1‑150 (MQ=255)
                           tCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCt                                                                                                                          >  2:757073/1‑150 (MQ=255)
                             attattTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCa                                                                                                                        >  2:1191573/1‑150 (MQ=255)
                             attattTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCa                                                                                                                        >  2:940769/1‑150 (MQ=255)
                             attattTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCa                                                                                                                        >  2:2222993/1‑150 (MQ=255)
                             attattTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCa                                                                                                                        >  2:1812332/1‑150 (MQ=255)
                             attattTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCa                                                                                                                        <  1:739878/150‑1 (MQ=255)
                               tattTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCACTGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCaga                                                                                                                      <  1:852055/150‑1 (MQ=255)
                               tattTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGTGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAAGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCaga                                                                                                                      <  2:538906/150‑1 (MQ=255)
                                attTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTGCTTAGCTCagag                                                                                                                     >  1:287190/1‑150 (MQ=255)
                                 tttCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGt                                                                                                                    >  2:2503399/1‑150 (MQ=255)
                                 tttCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGt                                                                                                                    <  2:440626/150‑1 (MQ=255)
                                  ttCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTg                                                                                                                   >  1:541031/1‑150 (MQ=255)
                                    cGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGaa                                                                                                                 <  1:695943/150‑1 (MQ=255)
                                          gCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCACTGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAACTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTaa                                                                                                           <  1:1502132/150‑1 (MQ=255)
                                           cTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAAc                                                                                                          <  2:1556800/150‑1 (MQ=255)
                                           cTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAAc                                                                                                          <  2:1697402/150‑1 (MQ=255)
                                           cTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAAc                                                                                                          <  1:788453/150‑1 (MQ=255)
                                           cTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAAc                                                                                                          <  2:66559/150‑1 (MQ=255)
                                             tATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCt                                                                                                        >  1:1315308/1‑150 (MQ=255)
                                              aTCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTa                                                                                                       <  1:1474710/150‑1 (MQ=255)
                                               acAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTac                                                                                                      <  1:1068866/149‑2 (MQ=255)
                                                 aGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCt                                                                                                    <  2:1618405/150‑1 (MQ=255)
                                                 aGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCt                                                                                                    <  1:1113385/150‑1 (MQ=255)
                                                 aGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCt                                                                                                    >  1:227156/1‑150 (MQ=255)
                                                   tCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGc                                                                                                  <  1:1130565/150‑1 (MQ=255)
                                                    ccGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGcc                                                                                                 <  1:2253690/150‑1 (MQ=255)
                                                        gtgtAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGGTTAACCTATCTGCCCAtt                                                                                             >  2:1077743/1‑150 (MQ=255)
                                                        gtgtAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGGTTAACCTATCTGCCCAtt                                                                                             >  2:1728525/1‑150 (MQ=255)
                                                        gtgtAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCAtt                                                                                             >  2:1302818/1‑150 (MQ=255)
                                                           tAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTccc                                                                                          >  1:1608434/1‑150 (MQ=255)
                                                               cTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCaaa                                                                                      >  1:1239348/1‑150 (MQ=255)
                                                                tGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTGCTTAGCTCAGAGTGAAGATTAACCTCTCTGCCCATTCCCCAAAt                                                                                     >  1:2018399/1‑150 (MQ=255)
                                                                      cGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTaa                                                                               <  2:607920/150‑1 (MQ=255)
                                                                      cGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTaa                                                                               >  1:1284772/1‑150 (MQ=255)
                                                                       ggTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAAc                                                                              >  1:316170/1‑150 (MQ=255)
                                                                         tAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAAcgc                                                                            >  2:2433242/1‑150 (MQ=255)
                                                                           aTCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACgcgg                                                                          >  2:1837395/1‑150 (MQ=255)
                                                                           aTCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACgcgg                                                                          >  2:1863813/1‑150 (MQ=255)
                                                                            tCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACgcggc                                                                         >  1:1928525/1‑150 (MQ=255)
                                                                             cAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACgcggcg                                                                        <  1:1077105/150‑1 (MQ=255)
                                                                             cAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACgcggcg                                                                        <  1:963049/150‑1 (MQ=255)
                                                                                      aTGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAActtc                                                               >  2:262580/1‑150 (MQ=255)
                                                                                      aTGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAActtc                                                               >  2:1956615/1‑150 (MQ=255)
                                                                                        gCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAActtctt                                                             <  2:1464783/150‑1 (MQ=255)
                                                                                          gACGCTGTCGCGTCTTATCAGACCAGGAGCAATTTCCAAATGTAGGCCGGATAAGGCGTTTAAGCCGCATCCGGCATTTGCATAGCTCAGATTGAAGATTAAACTATCAGCCCAATCCCCAAATGTTTAAACCGGCGCTTAAATTATTcc                                                           >  1:2582294/1‑150 (MQ=37)
                                                                                            cGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTg                                                         <  1:24096/150‑1 (MQ=255)
                                                                                                gTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTa                                                     >  1:1683850/1‑150 (MQ=255)
                                                                                                gTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGAGAAGATTAACCTATCAGCCCATTCCCCAAATGTTTAACGCGGCGCATAACTTCTTCCTGCTTa                                                     >  1:1815780/1‑150 (MQ=255)
                                                                                                   gcgTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCa                                                  >  2:2110128/1‑150 (MQ=255)
                                                                                                    cgTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTGAACTTCTTCCTGCTTAcccg                                                 >  2:2126072/1‑148 (MQ=255)
                                                                                                           tCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTaaa                                          <  1:1202795/150‑1 (MQ=255)
                                                                                                           tCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTaaa                                          <  1:436511/150‑1 (MQ=255)
                                                                                                           tCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTCAACTTCTTCCTGCTTACCAGCGTTaaa                                          <  1:1191573/150‑1 (MQ=255)
                                                                                                             aGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACg                                        >  2:1275209/1‑150 (MQ=255)
                                                                                                                      gCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCAt                               >  1:982247/1‑150 (MQ=255)
                                                                                                                       cAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATc                              >  1:600039/1‑150 (MQ=255)
                                                                                                                          ttGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCgg                           <  1:2489068/150‑1 (MQ=255)
                                                                                                                          ttGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCgg                           <  1:2418068/150‑1 (MQ=255)
                                                                                                                          ttGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCgg                           <  1:2103951/150‑1 (MQ=255)
                                                                                                                             ccAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCt                        >  2:700936/1‑150 (MQ=255)
                                                                                                                                  tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTa                   <  2:1283670/150‑1 (MQ=255)
                                                                                                                                     aGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAat                <  1:503568/150‑1 (MQ=255)
                                                                                                                                     aGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAat                <  1:2527009/150‑1 (MQ=255)
                                                                                                                                       gCCGGATGCGGCGTAAACGCCTTATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAatat              >  2:584428/1‑150 (MQ=255)
                                                                                                                                       gCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAatat              >  1:1756445/1‑150 (MQ=255)
                                                                                                                                       gCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAatat              >  1:1766259/1‑150 (MQ=255)
                                                                                                                                       gCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAatat              >  1:1830634/1‑150 (MQ=255)
                                                                                                                                        ccGGATGCGGCGTAAACGCCTTATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATc             >  2:1980768/1‑150 (MQ=255)
                                                                                                                                        ccGGATGCGGCGTAAACGCCTTATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATc             >  2:1219330/1‑150 (MQ=255)
                                                                                                                                        ccGGATGCGGCGTAAACGCCTTATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATc             >  2:3194/1‑150 (MQ=255)
                                                                                                                                         cGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATcc            <  1:2503399/150‑1 (MQ=255)
                                                                                                                                         cGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATcc            <  1:378616/150‑1 (MQ=255)
                                                                                                                                          ggATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAACTGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCg           <  2:2325219/150‑1 (MQ=255)
                                                                                                                                          ggATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCg           <  1:827943/150‑1 (MQ=255)
                                                                                                                                             tAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGcac        >  2:724271/1‑150 (MQ=255)
                                                                                                                                             tAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGcac        >  2:1488398/1‑150 (MQ=255)
                                                                                                                                                   gTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGCACACgcgg  <  1:208783/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGCACACGCGG  >  NC_000913/4460334‑4460630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: