Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,460,484 T→A 6.3% intergenic (‑120/+38) nrdG ← / ← nrdD anaerobic ribonucleotide reductase activating protein/anaerobic ribonucleoside‑triphosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,460,4840TA 6.3% 811.7 / 29.3 222intergenic (‑120/+38)nrdG/nrdDanaerobic ribonucleotide reductase activating protein/anaerobic ribonucleoside‑triphosphate reductase
Reads supporting (aligned to +/- strand):  ref base T (129/79);  new base A (11/3);  total (140/82)
Fisher's exact test for biased strand distribution p-value = 2.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 227 total aligned reads displayed.

GTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGCACACGCGGCGA  >  NC_000913/4460335‑4460633
                                                                                                                                                     |                                                                                                                                                     
 tCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTa                                                                                                                                                      >  2:162600/1‑150 (MQ=255)
 tCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTa                                                                                                                                                      >  1:1793515/1‑150 (MQ=255)
     cAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                                  >  1:722729/1‑150 (MQ=255)
     cAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                                  <  2:2369493/150‑1 (MQ=255)
        gATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATGCGGCGTAAAcgcctta                                                                                                                                               <  2:1078479/150‑4 (MQ=38)
        gATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATGCGGCGTAAAcgcctta                                                                                                                                               <  2:2312584/150‑4 (MQ=38)
          tAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                                                                                             >  1:1658339/1‑150 (MQ=255)
             tACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                          >  1:371331/1‑150 (MQ=255)
                tgatgaTAATTCATTATTTCGCCTGGCTTATAAGTCCGAGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGTCCGGATAAGGCGTTTACGCCGCATCCGGCAt                                                                                                                                       >  1:559172/1‑150 (MQ=255)
                 gatgatAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAtt                                                                                                                                      >  1:1724146/1‑150 (MQ=255)
                 gatgatAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAtt                                                                                                                                      >  2:740457/1‑150 (MQ=255)
                 gatgatAAATCATTATTACGCCTGGCTTATAAGTCCGCGAGTAGGCAGCATCCGGTAATCAATGCCTGATTCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGAAGGCCGGATAAGGAGTTTACGCCGCATCCGGCAtt                                                                                                                                      >  2:1849561/1‑150 (MQ=37)
                    gatAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGc                                                                                                                                   >  2:783437/1‑150 (MQ=255)
                    gatAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGc                                                                                                                                   >  2:1130329/1‑150 (MQ=255)
                    gatAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGc                                                                                                                                   >  2:119729/1‑150 (MQ=255)
                    gatAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTAATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGTCGTTTACGCCGCATCCGGCATTTGc                                                                                                                                   >  2:1587324/1‑150 (MQ=255)
                        aTTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAg                                                                                                                               >  2:366001/1‑150 (MQ=255)
                          tCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCt                                                                                                                             >  1:114723/1‑150 (MQ=255)
                          tCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCt                                                                                                                             >  1:714681/1‑150 (MQ=255)
                          tCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCt                                                                                                                             >  1:1891175/1‑150 (MQ=255)
                          tCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCt                                                                                                                             >  2:757073/1‑150 (MQ=255)
                          tCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCt                                                                                                                             >  1:714641/1‑150 (MQ=255)
                          tCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCt                                                                                                                             >  2:563677/1‑150 (MQ=255)
                           cATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTc                                                                                                                            >  1:1118358/1‑150 (MQ=255)
                            attattTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCa                                                                                                                           >  2:2222993/1‑150 (MQ=255)
                            attattTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCa                                                                                                                           >  2:1812332/1‑150 (MQ=255)
                            attattTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCa                                                                                                                           >  2:940769/1‑150 (MQ=255)
                            attattTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCa                                                                                                                           >  2:436511/1‑150 (MQ=255)
                              tattTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGTGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAAGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCaga                                                                                                                         <  2:538906/150‑1 (MQ=255)
                              tattTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCaga                                                                                                                         <  2:1683345/150‑1 (MQ=255)
                              tattTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCaga                                                                                                                         <  1:321686/150‑1 (MQ=255)
                               attTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCagag                                                                                                                        >  1:287208/1‑150 (MQ=255)
                                tttCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGt                                                                                                                       >  2:2503399/1‑150 (MQ=255)
                                tttCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGt                                                                                                                       >  2:253376/1‑150 (MQ=255)
                                 ttCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTg                                                                                                                      >  1:540959/1‑150 (MQ=255)
                                       tGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGAtt                                                                                                                <  1:2521446/150‑1 (MQ=255)
                                         gCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCACTGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAACTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTaa                                                                                                              <  1:1502132/150‑1 (MQ=255)
                                          cTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGATCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAAc                                                                                                             <  1:788501/150‑1 (MQ=255)
                                          cTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAAc                                                                                                             <  2:1903957/150‑1 (MQ=255)
                                          cTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAAc                                                                                                             <  2:66559/150‑1 (MQ=255)
                                            tATCAGTCCGCGTGTAGGCTGCATCCGTTAATCAAAGCCTGATGCGACGCTGTCGCGACTTATCAGACCTGGAGCAATTGCCAAATGTAGTCCGGATAAGGCGTTTACGCCGCATCCGGAATATGCTTAGCTCAGAGTGAAGATTAACCt                                                                                                           >  1:1315289/1‑150 (MQ=38)
                                            tATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCt                                                                                                           >  1:1315308/1‑150 (MQ=255)
                                             aTCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTa                                                                                                          <  1:1474710/150‑1 (MQ=255)
                                              tCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTAt                                                                                                         <  1:1069004/150‑1 (MQ=255)
                                                  tCCGCGTGTAGGCTGCATCCTGTTATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCTGAGTGAAGATTAACCTATCTGc                                                                                                     <  1:1197368/150‑1 (MQ=255)
                                                         gtAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTcc                                                                                              >  1:1829937/1‑150 (MQ=255)
                                                         gtAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTcc                                                                                              <  1:499132/150‑1 (MQ=255)
                                                            ggCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCa                                                                                           >  1:387161/1‑150 (MQ=255)
                                                               tGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTGCTTAGCTCAGAGTGAAGATTAACCTCTCTGCCCATTCCCCAAAt                                                                                        >  1:2018399/1‑150 (MQ=255)
                                                                  aTCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGtt                                                                                     >  2:1525060/1‑150 (MQ=255)
                                                                   tCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGttt                                                                                    >  1:632764/1‑150 (MQ=255)
                                                                   tCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGttt                                                                                    <  2:1829937/150‑1 (MQ=255)
                                                                     cGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTaa                                                                                  >  1:2559386/1‑150 (MQ=255)
                                                                     cGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTaa                                                                                  <  2:607592/150‑1 (MQ=255)
                                                                     cGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTaa                                                                                  >  1:309228/1‑150 (MQ=255)
                                                                      ggTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCATACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAAc                                                                                 >  1:451377/1‑150 (MQ=255)
                                                                      ggTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAAc                                                                                 >  2:160859/1‑150 (MQ=255)
                                                                        tAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAAcgc                                                                               >  2:2433242/1‑150 (MQ=255)
                                                                           tCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACgcggc                                                                            >  1:1928525/1‑150 (MQ=255)
                                                                            cAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACgcggcg                                                                           >  1:1222167/1‑150 (MQ=255)
                                                                             aaTGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGgcgc                                                                          >  2:1287323/1‑150 (MQ=255)
                                                                                     aTGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAActtc                                                                  >  2:262578/1‑150 (MQ=255)
                                                                                      tGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAAAGCGGCGCTTAActtct                                                                 >  1:2433358/1‑150 (MQ=255)
                                                                                       gCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAActtctt                                                                <  1:844655/150‑1 (MQ=255)
                                                                                         gACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATTTAGGCCGGATAAGGCGTTTACGCCGCAAACGGCATTTGCTTAGCTAAGATTGAAGATTAACCAATCTTCCCAATACCCAAATGTTTAACGCGGCGCTTAACTTATTcc                                                              >  1:508556/1‑150 (MQ=38)
                                                                                         gACGCTGTCGCGTCTTATCAGACCAGGAGCAATTTCCAAATGTAGGCCGGATAAGGCGTTTAAGCCGCATCCGGCATTTGCATAGCTCAGATTGAAGATTAAACTATCAGCCCAATCCCCAAATGTTTAAACCGGCGCTTAAATTATTcc                                                              >  1:2582294/1‑150 (MQ=37)
                                                                                           cGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTg                                                            <  1:24108/150‑1 (MQ=255)
                                                                                                   cgTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTGAACTTCTTCCTGCTTAcccg                                                    >  2:2126072/1‑148 (MQ=255)
                                                                                                      cTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGt                                                 >  2:1269419/1‑150 (MQ=255)
                                                                                                        tATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTa                                               >  1:2233717/1‑150 (MQ=255)
                                                                                                          tCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTaaa                                             <  2:1724146/150‑1 (MQ=255)
                                                                                                          tCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTaaa                                             <  1:940769/150‑1 (MQ=255)
                                                                                                          tCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTaaa                                             <  1:950150/150‑1 (MQ=255)
                                                                                                          tCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTaaa                                             <  1:740457/150‑1 (MQ=255)
                                                                                                                     gCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCAt                                  >  1:982158/1‑150 (MQ=255)
                                                                                                                         ttGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCgg                              <  1:2103951/150‑1 (MQ=255)
                                                                                                                         ttGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCgg                              <  1:2489068/150‑1 (MQ=255)
                                                                                                                         ttGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCgg                              <  1:1264777/150‑1 (MQ=255)
                                                                                                                            ccAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCt                           >  2:700936/1‑150 (MQ=255)
                                                                                                                              aaaTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTACCTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTAc                         <  2:2062273/150‑1 (MQ=255)
                                                                                                                              aaaTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTAc                         <  2:1783426/150‑1 (MQ=255)
                                                                                                                                      gCCGGATGCGGCGTAAACGCCTTATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAatat                 >  2:584428/1‑150 (MQ=255)
                                                                                                                                      gCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAatat                 >  1:1766259/1‑150 (MQ=255)
                                                                                                                                       ccGGATGCGGCGTAAACGCCTTATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATc                >  2:1282574/1‑150 (MQ=255)
                                                                                                                                       ccGGATGCGGCGTAAACGCCTTATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATc                >  1:1679260/1‑150 (MQ=255)
                                                                                                                                       ccGGATGCGGCGTAAACGCCTTATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATc                >  2:1416312/1‑150 (MQ=255)
                                                                                                                                       ccGGATGCGGCGTAAACGCCTTATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATc                >  2:3194/1‑150 (MQ=255)
                                                                                                                                       ccGGATGCGGCGTAAACGCCTTATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATc                >  2:1980768/1‑150 (MQ=255)
                                                                                                                                        cGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATcc               <  1:253376/150‑1 (MQ=255)
                                                                                                                                          gATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGc             >  1:433122/1‑150 (MQ=255)
                                                                                                                                            tAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTATACGGACGTGCATCCGGGCTACCTAAATATCCGcac           >  2:2013247/1‑150 (MQ=255)
                                                                                                                                            tAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGcac           >  2:1488398/1‑150 (MQ=255)
                                                                                                                                            tAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGcac           >  2:724271/1‑150 (MQ=255)
                                                                                                                                               ggCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGCACAcg        >  1:2269775/1‑150 (MQ=255)
                                                                                                                                                  gTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGCACACgcgg     >  2:1017817/1‑150 (MQ=255)
                                                                                                                                                  gTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGCACACgcgg     <  1:111258/150‑1 (MQ=255)
                                                                                                                                                  gTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGCACACgcgg     <  1:208783/150‑1 (MQ=255)
                                                                                                                                                     tACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGCACACGCGGCGa  <  1:2373787/150‑1 (MQ=255)
                                                                                                                                                     tACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGCACACGCGGCGa  <  1:1047489/150‑1 (MQ=255)
                                                                                                                                                     tACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGCACACGCGGCGa  <  1:2221705/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGCACACGCGGCGA  >  NC_000913/4460335‑4460633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: