Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,473,927 C→A 100% A43S (GCC→TCC)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,473,9270CA100.0% 607.7 / NA 158A43S (GCC→TCC) bdcAc‑di‑GMP‑binding biofilm dispersal mediator protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (86/72);  total (86/72)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 158 total aligned reads displayed.

GGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTA  >  NC_000913/4473780‑4474073
                                                                                                                                                   |                                                                                                                                                  
gggCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAAt                                                                                                                                                  <  1:2135508/150‑1 (MQ=255)
gggCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAAt                                                                                                                                                  <  1:1493163/150‑1 (MQ=255)
     tCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTc                                                                                                                                             <  1:1111621/150‑1 (MQ=255)
     tCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTc                                                                                                                                             <  1:1111637/150‑1 (MQ=255)
       gCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGGCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGa                                                                                                                                           >  1:1896219/1‑150 (MQ=255)
          aaaGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGAccc                                                                                                                                        <  2:389909/150‑1 (MQ=255)
            aGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCGGGCGTTTAGCGGAATCTTTCGACCCCg                                                                                                                                      <  2:2160039/150‑1 (MQ=255)
             gACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGc                                                                                                                                     <  1:523860/150‑1 (MQ=255)
              aCGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTTTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGATAAGCGGAATCTTTCGACCCCGCa                                                                                                                                    >  1:1203436/1‑150 (MQ=255)
              aCGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCa                                                                                                                                    >  1:470879/1‑150 (MQ=255)
                 ccAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAg                                                                                                                                 >  2:1972538/1‑150 (MQ=255)
                  cAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAgg                                                                                                                                >  2:760410/1‑150 (MQ=255)
                  cAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAgg                                                                                                                                <  2:1079236/150‑1 (MQ=255)
                  cAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCAGAgg                                                                                                                                >  2:560490/1‑150 (MQ=255)
                  cAATACCTGCATTTACCACCAGGATATCCAATGCGCCGATCTTACGATCGACATAAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCATTCTATTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAgt                                                                                                                                <  2:1079200/150‑2 (MQ=255)
                   aaTACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGt                                                                                                                               >  1:516346/1‑150 (MQ=255)
                   aaTACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGt                                                                                                                               <  1:92618/150‑1 (MQ=255)
                   aaTACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGt                                                                                                                               >  1:1811802/1‑150 (MQ=255)
                       ccTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAAt                                                                                                                           >  2:1454657/1‑150 (MQ=255)
                        cTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATc                                                                                                                          <  2:2318030/150‑1 (MQ=255)
                        cTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATc                                                                                                                          <  2:1218438/150‑1 (MQ=255)
                         tGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCg                                                                                                                         >  1:1705935/1‑150 (MQ=255)
                         tGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCg                                                                                                                         >  1:1507739/1‑150 (MQ=255)
                         tGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCg                                                                                                                         >  2:1689328/1‑150 (MQ=255)
                         tGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCg                                                                                                                         >  1:416454/1‑150 (MQ=255)
                           cATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTa                                                                                                                       >  2:2133195/1‑150 (MQ=255)
                           cATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTa                                                                                                                       >  2:2509421/1‑150 (MQ=255)
                                 ccaccaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTgg                                                                                                                 <  1:2044186/150‑1 (MQ=255)
                                  caccaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGc                                                                                                                <  2:1660606/150‑1 (MQ=255)
                                  caccaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCGTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGc                                                                                                                <  2:443442/150‑1 (MQ=255)
                                    ccaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGccc                                                                                                              >  1:1418115/1‑150 (MQ=255)
                                    ccaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGccc                                                                                                              >  1:2181991/1‑150 (MQ=255)
                                       ggATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCAt                                                                                                           >  1:748055/1‑150 (MQ=255)
                                        gATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATc                                                                                                          <  1:2170843/150‑1 (MQ=255)
                                        gATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATc                                                                                                          >  2:2183974/1‑150 (MQ=255)
                                           atCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGt                                                                                                       <  1:922342/150‑1 (MQ=255)
                                           atCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGt                                                                                                       <  1:920741/150‑1 (MQ=255)
                                           atCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGt                                                                                                       <  1:2288151/150‑1 (MQ=255)
                                              cAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGAc                                                                                                    <  1:465790/150‑1 (MQ=255)
                                                     ccgcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAacga                                                                                             <  1:318202/150‑1 (MQ=255)
                                                      cgcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAacgac                                                                                            <  1:1686792/150‑1 (MQ=255)
                                                                accgaCATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGc                                                                                  <  2:2403370/148‑1 (MQ=255)
                                                                aacgaCATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGc                                                                                  <  2:154062/150‑1 (MQ=255)
                                                                     cATCACTGCCAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCCGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATCGGTGAATCGTACATGGGCCCCCTGGGTGACGAAACGACGTACGATAGCGGCAc                                                                             <  1:938789/150‑1 (MQ=255)
                                                                      aTCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCAcc                                                                            <  2:2430167/150‑1 (MQ=255)
                                                                        cAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGa                                                                          >  2:1184368/1‑150 (MQ=255)
                                                                        cAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGa                                                                          >  2:162180/1‑150 (MQ=255)
                                                                         aaTGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGAt                                                                         >  1:2463546/1‑150 (MQ=255)
                                                                         aaTGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGAt                                                                         >  1:1254110/1‑150 (MQ=255)
                                                                             aCAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTAGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCa                                                                     >  1:2252647/1‑150 (MQ=255)
                                                                                gCGTCTCTGTCAGCACTATCTGTGAAGACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGa                                                                  <  1:393488/150‑1 (MQ=255)
                                                                                  gTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACt                                                                <  2:865616/150‑1 (MQ=255)
                                                                                         tCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCg                                                         >  2:16668/1‑150 (MQ=255)
                                                                                          cAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGa                                                        <  1:2184038/150‑1 (MQ=255)
                                                                                           aGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAg                                                       >  1:1235681/1‑150 (MQ=255)
                                                                                           aGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGCGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAg                                                       >  1:38411/1‑150 (MQ=255)
                                                                                           aGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATATTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAg                                                       >  1:759781/1‑150 (MQ=255)
                                                                                            gCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAgg                                                      >  2:2572167/1‑150 (MQ=255)
                                                                                             cACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTTCCAGGCGTTTAGCGGAATATTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAAACACGTACGATAGCGGCAACGATACCACGACTGCCACCGAGGa                                                     >  1:1434774/1‑150 (MQ=255)
                                                                                             cACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGa                                                     >  1:2045173/1‑150 (MQ=255)
                                                                                                tATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATga                                                  >  2:2471916/1‑150 (MQ=255)
                                                                                                tATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCAACGGTGACGAAACGACGTACGATAGCGGCACCGATAACACCACTGCCACCGAGGATga                                                  >  1:851712/1‑150 (MQ=255)
                                                                                                 aTCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATgag                                                 >  2:543427/1‑150 (MQ=255)
                                                                                                  tCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATgaga                                                <  2:2548430/150‑1 (MQ=255)
                                                                                                  tCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATgaga                                                >  2:1398679/1‑150 (MQ=255)
                                                                                                  tCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCAACGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATgaga                                                >  1:593548/1‑150 (MQ=255)
                                                                                                  tCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAAACGTACATTGGCCCCAACGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACAGCCACCGAGGATgaga                                                >  1:1005215/1‑150 (MQ=255)
                                                                                                  tCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCAAAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTAAGATAGCGGCACCGATACCACGACAGCCACCGAGGATgaga                                                >  1:1824651/1‑150 (MQ=255)
                                                                                                  tCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTAGACCCCGCATAGGTGAATCGTACATTTGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATgaga                                                >  1:1771089/1‑150 (MQ=255)
                                                                                                     gtgAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACt                                             <  1:1059949/150‑1 (MQ=255)
                                                                                                       gAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGt                                           <  1:115238/150‑1 (MQ=255)
                                                                                                        aaTACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTc                                          >  2:688766/1‑150 (MQ=255)
                                                                                                        aaTACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTc                                          <  2:1127567/150‑1 (MQ=255)
                                                                                                        aaTACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTc                                          >  2:1986549/1‑150 (MQ=255)
                                                                                                           aCTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTa                                       <  1:300650/150‑1 (MQ=255)
                                                                                                            ctgctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTAc                                      <  1:2189680/150‑1 (MQ=255)
                                                                                                            ctgctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTAc                                      <  2:1502384/150‑1 (MQ=255)
                                                                                                               ctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTg                                   >  2:2423908/1‑150 (MQ=255)
                                                                                                                     cTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAg                             >  2:2174571/1‑150 (MQ=255)
                                                                                                                     cTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAg                             >  1:1613636/1‑150 (MQ=255)
                                                                                                                     cTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAg                             >  2:757223/1‑150 (MQ=255)
                                                                                                                      tCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAgc                            >  2:1130275/1‑150 (MQ=255)
                                                                                                                         aGTCTCTTGTGCCAGGCGTTTAGCGTAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGcc                         <  1:1563287/150‑1 (MQ=255)
                                                                                                                         aGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGcc                         <  2:1472209/150‑1 (MQ=255)
                                                                                                                         aGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGcc                         <  2:2536342/150‑1 (MQ=255)
                                                                                                                             tctTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGTATGAGAACTGTCTTACCTGTAAAAGCGCCCATa                     <  2:2550225/150‑1 (MQ=255)
                                                                                                                              ctTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATaa                    <  2:359980/150‑1 (MQ=255)
                                                                                                                              ctTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATaa                    <  2:704766/150‑1 (MQ=255)
                                                                                                                                    cctggCGTTTCGCGGAATCTTTCGACCCCGCTTAGGTTAATGGTACATGGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGTGGTTGTGAACTGTCTTACCTTTAAAAGCGCCCATAAGGACTc              <  1:777551/147‑1 (MQ=255)
                                                                                                                                       ggCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCtt           >  2:1103790/1‑150 (MQ=255)
                                                                                                                                       ggCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCtt           >  2:498662/1‑150 (MQ=255)
                                                                                                                                       ggCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCtt           >  2:651717/1‑150 (MQ=255)
                                                                                                                                        gCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTg          >  1:2524281/1‑150 (MQ=255)
                                                                                                                                         cGTTTAGCGGAATATTTCGACCCCGCATAGGTGAATCGTACAATTGCCCCATCGGTGACGAAAACACCTACGATAGCGGCACCGAAACCACCACATCCACCGAGGATGAGAACTGTCATACCAGTAAAAGCGCCCAAAAGGACACCTTGa         >  2:2101362/1‑150 (MQ=255)
                                                                                                                                           tttAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGAtt       <  1:1940546/150‑1 (MQ=255)
                                                                                                                                              aGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATttat    <  1:2509421/150‑1 (MQ=255)
                                                                                                                                               gCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATttatt   >  2:1779737/1‑150 (MQ=255)
                                                                                                                                                cGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATttatta  >  1:1184105/1‑150 (MQ=255)
                                                                                                                                                cGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATttatta  <  1:1454657/150‑1 (MQ=255)
                                                                                                                                                cGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATttatta  <  1:895722/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTA  >  NC_000913/4473780‑4474073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: