Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,632,323 C→T 8.6% H531Y (CAT→TAT)  slt → lytic murein transglycosylase, soluble

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,632,3230CT 8.6% 527.6 / 28.4 162H531Y (CAT→TAT) sltlytic murein transglycosylase, soluble
Reads supporting (aligned to +/- strand):  ref base C (73/75);  new base T (7/7);  total (80/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 166 total aligned reads displayed.

ACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAAC  >  NC_000913/4632174‑4632472
                                                                                                                                                     |                                                                                                                                                     
aCGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGNGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGAccc                                                                                                                                                       >  2:2012322/1‑150 (MQ=255)
      ttttCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACgg                                                                                                                                                 >  1:1062831/1‑150 (MQ=255)
        ttCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTg                                                                                                                                               >  2:1919222/1‑150 (MQ=255)
          cAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGaa                                                                                                                                             <  1:1013979/150‑1 (MQ=255)
          cAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGaa                                                                                                                                             <  2:1708667/150‑1 (MQ=255)
           aaaCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAg                                                                                                                                            <  2:1883181/150‑1 (MQ=255)
            ttcGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAgt                                                                                                                                           >  2:2462532/3‑149 (MQ=255)
            aaCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGa                                                                                                                                           >  2:1758406/1‑150 (MQ=255)
            aaCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGa                                                                                                                                           >  1:1107604/1‑150 (MQ=255)
            aaCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGa                                                                                                                                           >  1:2134112/1‑150 (MQ=255)
             aCGCTACACCAGCGGTAAGGAGATCCCGCATAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGTTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGAt                                                                                                                                          <  2:1221968/150‑1 (MQ=255)
             aCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCTGTTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGAt                                                                                                                                          <  2:1289946/150‑1 (MQ=255)
             aCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGTTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGAt                                                                                                                                          <  2:1147939/150‑1 (MQ=255)
             aCGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGTTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGAt                                                                                                                                          <  2:2084549/150‑1 (MQ=255)
              cGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATg                                                                                                                                         <  2:1682859/150‑1 (MQ=255)
                cTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGtt                                                                                                                                       <  2:380006/150‑1 (MQ=255)
                  acacCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTtct                                                                                                                                     <  2:849019/150‑1 (MQ=255)
                    acCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTctct                                                                                                                                   >  1:2301382/1‑150 (MQ=255)
                    acCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTctct                                                                                                                                   >  1:2094686/1‑150 (MQ=255)
                        gCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTc                                                                                                                               <  2:72566/150‑1 (MQ=255)
                        gCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTc                                                                                                                               <  1:2570124/150‑1 (MQ=255)
                          ggTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTccc                                                                                                                             >  2:2286851/1‑150 (MQ=255)
                          ggTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTccc                                                                                                                             <  2:262200/150‑1 (MQ=255)
                             aaGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGt                                                                                                                          >  1:1470133/1‑150 (MQ=255)
                               ggAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCTATACGGTGAAGATGTTCTCTATTCCCGGTta                                                                                                                        >  1:972639/1‑150 (MQ=255)
                               ggAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCTATACGGTGAAGATGTTCTCTATTCCCGGTta                                                                                                                        >  1:1901578/1‑150 (MQ=255)
                               ggAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCTATACGGTGAAGATGTTCTCTATTCCCGGTta                                                                                                                        >  2:2049713/1‑150 (MQ=255)
                               ggAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCTATACGGTGAAGAGGTGCTCTAGTCCCGGTta                                                                                                                        >  1:608402/1‑150 (MQ=255)
                                 agaTCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTtata                                                                                                                      >  2:695492/1‑150 (MQ=255)
                                  gaTCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAg                                                                                                                     <  2:1940101/150‑1 (MQ=255)
                                  gaTCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAg                                                                                                                     >  2:1519268/1‑150 (MQ=255)
                                   aTCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGc                                                                                                                    >  2:1004928/1‑150 (MQ=255)
                                    tCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGGTGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCa                                                                                                                   <  1:420789/150‑1 (MQ=255)
                                    tCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCa                                                                                                                   <  2:2556674/150‑1 (MQ=255)
                                    tCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCa                                                                                                                   <  1:2310969/150‑1 (MQ=255)
                                    tCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCa                                                                                                                   <  1:1336277/150‑1 (MQ=255)
                                        gCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTcc                                                                                                               <  2:231625/150‑1 (MQ=255)
                                          aaaGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTg                                                                                                             <  1:898630/150‑1 (MQ=255)
                                          aaaGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTg                                                                                                             <  1:2104627/150‑1 (MQ=255)
                                           aaGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTgg                                                                                                            <  1:1508627/150‑1 (MQ=255)
                                            aGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTggg                                                                                                           <  2:1557407/150‑1 (MQ=255)
                                                 tGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAAtt                                                                                                      <  1:1491847/150‑1 (MQ=255)
                                                   cGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGc                                                                                                    <  2:2236285/150‑1 (MQ=255)
                                                    gATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCt                                                                                                   <  2:2503805/150‑1 (MQ=255)
                                                     aTGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTg                                                                                                  >  1:2030231/1‑150 (MQ=255)
                                                        gCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGAt                                                                                               <  1:1019258/150‑1 (MQ=255)
                                                          gATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATcc                                                                                             <  2:1080692/150‑1 (MQ=255)
                                                             tGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGa                                                                                          >  2:1986817/1‑150 (MQ=255)
                                                                  gTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGa                                                                                     >  2:2027952/1‑150 (MQ=255)
                                                                   tCAGGAGAGCGCCTGGACTCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGaa                                                                                    <  2:1848815/150‑1 (MQ=255)
                                                                   tCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGaa                                                                                    <  2:1306283/150‑1 (MQ=255)
                                                                   tCAGGAGAGCGCCGGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGCTTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGaa                                                                                    <  2:929581/150‑1 (MQ=255)
                                                                      ggAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAatat                                                                                 >  1:1947039/1‑150 (MQ=255)
                                                                      ggAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAatat                                                                                 >  1:2210030/1‑150 (MQ=255)
                                                                      ggAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAatat                                                                                 >  1:771019/1‑150 (MQ=255)
                                                                      ggAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAatat                                                                                 >  1:807800/1‑150 (MQ=255)
                                                                      ggAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAatat                                                                                 >  1:865878/1‑150 (MQ=255)
                                                                          agCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAAc                                                                             <  2:2523566/150‑1 (MQ=255)
                                                                             gcCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACAtt                                                                          >  2:746368/1‑150 (MQ=255)
                                                                              ccTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTg                                                                         >  2:572292/1‑150 (MQ=255)
                                                                                tGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGc                                                                       >  1:463358/1‑150 (MQ=255)
                                                                                 ggAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCa                                                                      <  2:781258/150‑1 (MQ=255)
                                                                                      ccGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGt                                                                 <  2:892249/150‑1 (MQ=255)
                                                                                       cGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGtt                                                                >  2:728030/1‑150 (MQ=255)
                                                                                           aGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCt                                                            >  1:1547601/1‑150 (MQ=255)
                                                                                           aGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCt                                                            >  1:1547794/1‑150 (MQ=255)
                                                                                               aaaTCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCaa                                                        >  2:518522/1‑150 (MQ=255)
                                                                                                 aTCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAata                                                      <  2:760599/150‑1 (MQ=255)
                                                                                                    aCCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGt                                                   >  2:506789/1‑150 (MQ=255)
                                                                                                       ggTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTa                                                >  1:926194/1‑150 (MQ=255)
                                                                                                        gTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTAt                                               >  2:616663/1‑150 (MQ=255)
                                                                                                             ggCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATcagca                                          <  1:695492/150‑1 (MQ=255)
                                                                                                              gCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATcagcag                                         >  2:1573909/1‑150 (MQ=255)
                                                                                                              gCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATcagcag                                         >  2:310645/1‑150 (MQ=255)
                                                                                                                   cGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTgg                                    >  2:2308131/1‑150 (MQ=255)
                                                                                                                      cTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTATGGCaa                                 >  1:1532740/1‑150 (MQ=255)
                                                                                                                       ttGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCaat                                >  2:1008995/1‑150 (MQ=255)
                                                                                                                       ttGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCaat                                >  2:396479/1‑150 (MQ=255)
                                                                                                                            gCAGATTCTGCCTGGTACAGCGACCTATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCg                           <  2:608402/150‑1 (MQ=255)
                                                                                                                            gCAGATTATGCCTGGTACAGCGACCTATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCg                           <  2:671787/150‑1 (MQ=255)
                                                                                                                            gCAGATTATGCCTGGTACAGCGACCTATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCg                           <  1:2049713/150‑1 (MQ=255)
                                                                                                                            gCAGATTATGCCTGGTACAGCGACCTATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCg                           <  2:972639/150‑1 (MQ=255)
                                                                                                                            gCAGATTATGCCTGGTACAGCGACCTATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCg                           <  2:606108/150‑1 (MQ=255)
                                                                                                                            gCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCg                           >  1:189359/1‑150 (MQ=255)
                                                                                                                             cAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGt                          >  2:459620/1‑150 (MQ=255)
                                                                                                                             cAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGt                          >  2:140473/1‑150 (MQ=255)
                                                                                                                                   aTGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTtc                    <  1:815225/150‑1 (MQ=255)
                                                                                                                                   aTGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTAGTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTtc                    <  1:815235/150‑1 (MQ=255)
                                                                                                                                    tGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTtct                   >  1:1302610/1‑150 (MQ=255)
                                                                                                                                     gCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTtctc                  <  2:819564/150‑1 (MQ=255)
                                                                                                                                      ccTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTctcc                 >  2:871202/1‑150 (MQ=255)
                                                                                                                                       cTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTctcct                >  2:1100440/1‑150 (MQ=255)
                                                                                                                                        tGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTctcctc               >  2:71263/1‑150 (MQ=255)
                                                                                                                                         ggTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTca              <  2:1837983/150‑1 (MQ=255)
                                                                                                                                            aCAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTcagca           <  2:14352/150‑1 (MQ=255)
                                                                                                                                            aCAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAAGTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTcagca           <  2:825747/150‑1 (MQ=255)
                                                                                                                                               gCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCt        >  2:2138962/1‑150 (MQ=255)
                                                                                                                                                cGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCtt       >  1:1900667/1‑150 (MQ=255)
                                                                                                                                                cGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGACAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCtt       <  2:1317763/150‑1 (MQ=255)
                                                                                                                                                 gACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTta      <  1:1155586/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAAC  >  NC_000913/4632174‑4632472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: