Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,546,696 A→T 6.3% S158R (AGT→AGA yddG ← aromatic amino acid exporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,546,6960AT 6.3% 699.0 / 25.8 191S158R (AGT→AGAyddGaromatic amino acid exporter
Reads supporting (aligned to +/- strand):  ref base A (86/93);  new base T (6/6);  total (92/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 199 total aligned reads displayed.

TCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGG  >  NC_000913/1546547‑1546841
                                                                                                                                                     |                                                                                                                                                 
tCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATaa                                                                                                                                                   <  2:892685/150‑1 (MQ=255)
tCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATaa                                                                                                                                                   <  2:683770/150‑1 (MQ=255)
tCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATaa                                                                                                                                                   <  1:2364180/150‑1 (MQ=255)
tCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATaa                                                                                                                                                   >  2:155360/1‑150 (MQ=255)
tCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATaa                                                                                                                                                   >  1:1401409/1‑150 (MQ=255)
  tGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACt                                                                                                                                                 >  2:2185713/1‑150 (MQ=255)
  tGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACt                                                                                                                                                 >  2:1028614/1‑150 (MQ=255)
        tgGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATgg                                                                                                                                           >  1:2525953/1‑150 (MQ=255)
        tgGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATgg                                                                                                                                           >  1:402490/1‑150 (MQ=255)
              aaGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCtggt                                                                                                                                     >  2:2458286/1‑150 (MQ=255)
                 aaaaTATTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGTTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTggtggt                                                                                                                                  <  1:19374/150‑1 (MQ=255)
                 aaaaTAGTAAACCCACAGACTTGCTCCCGTTAGCAGTACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTggtggt                                                                                                                                  <  1:803347/150‑1 (MQ=255)
                 aaaaTAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTggtggt                                                                                                                                  >  1:2008669/1‑150 (MQ=255)
                  aaaTAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGgtggtg                                                                                                                                 <  1:1560640/150‑1 (MQ=255)
                    aTAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGat                                                                                                                               <  1:1718523/150‑1 (MQ=255)
                      aGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGatat                                                                                                                             >  1:666067/1‑150 (MQ=255)
                      aGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGatat                                                                                                                             <  2:1874315/150‑1 (MQ=255)
                        tAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGAtatta                                                                                                                           >  2:670892/1‑150 (MQ=255)
                          aaCCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATattatt                                                                                                                         <  1:204717/150‑1 (MQ=255)
                            cccACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATtga                                                                                                                       <  2:2393280/150‑1 (MQ=255)
                            cccACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATtga                                                                                                                       <  2:2393495/150‑1 (MQ=255)
                               acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATATCTCAATGGGCTGGTGGTGATATTATtgatga                                                                                                                    >  2:1859730/1‑150 (MQ=255)
                               acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATATCTCAATGGGCTGGTGGTGATATTATtgatga                                                                                                                    >  2:499392/1‑150 (MQ=255)
                                    cTTGCTCCCGTTAGCATGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTtca                                                                                                               <  2:1124839/150‑1 (MQ=255)
                                         tCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATa                                                                                                          >  2:1690663/1‑150 (MQ=255)
                                          cccGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATaa                                                                                                         <  2:1303022/150‑1 (MQ=255)
                                                  cAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAAccc                                                                                                 >  1:972259/1‑150 (MQ=255)
                                                  cAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAAccc                                                                                                 >  2:2257970/1‑150 (MQ=255)
                                                  cAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAAccc                                                                                                 >  2:386672/1‑150 (MQ=255)
                                                   aGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCa                                                                                                >  1:864028/1‑150 (MQ=255)
                                                     gACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCAtt                                                                                              >  1:482227/1‑150 (MQ=255)
                                                     gACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCAtt                                                                                              <  1:995561/150‑1 (MQ=255)
                                                     gACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCAtt                                                                                              <  2:2318808/150‑1 (MQ=255)
                                                     gACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCAtt                                                                                              <  2:989482/150‑1 (MQ=255)
                                                     gACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCAtt                                                                                              <  1:1608549/150‑1 (MQ=255)
                                                     gACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCAtt                                                                                              <  1:1993592/150‑1 (MQ=255)
                                                            aCGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCg                                                                                       >  2:2535236/1‑150 (MQ=255)
                                                            aCGCTAATTCCAATAAATCCGCGTGAGTATTTATTAGTTACTGTTATATAGGCTGCCCAGATAAACGAACAAATGAAATCCAGGAAATAACTCAATGTGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAAAACAttttaacag                                                                                       >  1:2375178/1‑143 (MQ=255)
                                                              ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGcc                                                                                     >  2:2094182/1‑150 (MQ=255)
                                                              ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGcc                                                                                     >  2:212786/1‑150 (MQ=255)
                                                               gTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCt                                                                                    >  1:931642/1‑150 (MQ=255)
                                                                 aaTTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTaa                                                                                  <  1:2570358/150‑1 (MQ=255)
                                                                    tCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAacac                                                                               <  1:2407247/150‑1 (MQ=255)
                                                                      cATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACAccc                                                                             >  1:1199441/1‑150 (MQ=255)
                                                                      cATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACAccc                                                                             >  1:1199465/1‑150 (MQ=255)
                                                                       aTTAAATCCGCGTGCGTATTTATTCGCTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCa                                                                            >  2:1747742/1‑150 (MQ=255)
                                                                        ttAACTCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCaa                                                                           <  1:197705/150‑1 (MQ=255)
                                                                        ttAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCaa                                                                           <  1:2523075/150‑1 (MQ=255)
                                                                          aaaTCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACa                                                                         <  1:827413/150‑1 (MQ=255)
                                                                            aTCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGa                                                                       >  1:1832803/1‑150 (MQ=255)
                                                                                 cgtgcgtATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCcg                                                                  >  2:599982/1‑150 (MQ=255)
                                                                                     cgtATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCcgacga                                                              <  2:2284092/150‑1 (MQ=255)
                                                                                     cgtATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCcgacga                                                              <  2:2043852/150‑1 (MQ=255)
                                                                                     cgtATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACACAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCcgacga                                                              <  2:138893/150‑1 (MQ=255)
                                                                                       tatttattCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAgg                                                            <  2:1036267/150‑1 (MQ=255)
                                                                                       tatttattCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAgg                                                            <  2:2477428/150‑1 (MQ=255)
                                                                                        atttattCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCTTTGTCACCGCCTAACACCCAACAGACGCCGACGAggg                                                           <  1:222211/150‑1 (MQ=255)
                                                                                         tttattCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGc                                                          <  1:1035815/150‑1 (MQ=255)
                                                                                             ttCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCtaat                                                      >  1:1432313/1‑150 (MQ=255)
                                                                                             ttCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCtaat                                                      <  1:2284439/150‑1 (MQ=255)
                                                                                             ttCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCtaat                                                      >  2:1022920/1‑150 (MQ=255)
                                                                                                gTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCtaataat                                                   <  1:1496769/150‑1 (MQ=255)
                                                                                                gTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCtaataat                                                   <  1:259090/150‑1 (MQ=255)
                                                                                                 ttACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCtaataata                                                  >  1:1288929/1‑150 (MQ=255)
                                                                                                 ttACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCtaataata                                                  <  1:2471501/150‑1 (MQ=255)
                                                                                                  tACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCtaataataa                                                 <  2:355696/150‑1 (MQ=255)
                                                                                                     tgtgCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATcc                                              >  1:237937/1‑150 (MQ=255)
                                                                                                      gtgCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCa                                             <  2:2421919/150‑1 (MQ=255)
                                                                                                      gtgCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCa                                             <  2:2421940/150‑1 (MQ=255)
                                                                                                      gtgCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCa                                             <  2:1237413/150‑1 (MQ=255)
                                                                                                       tgCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAg                                            <  1:534052/150‑1 (MQ=255)
                                                                                                       tgCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAg                                            <  1:2470550/150‑1 (MQ=255)
                                                                                                       tgCAAAAGCCAGCCCAGAAAAACCCACCAATGAACGCCAGCAAAAAACTCAATGGTCTGCTCGTGATATTATTGATGATTACATAATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGAAGACGGCTAATAATAATCCAg                                            <  2:1433371/150‑1 (MQ=255)
                                                                                                        gCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAgg                                           <  2:1199641/150‑1 (MQ=255)
                                                                                                        gCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAgg                                           <  2:1072719/150‑1 (MQ=255)
                                                                                                          aaTAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTa                                         >  2:852118/1‑150 (MQ=255)
                                                                                                          aaTAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTa                                         >  2:1415188/1‑150 (MQ=255)
                                                                                                           aTAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTAc                                        <  1:2191591/150‑1 (MQ=255)
                                                                                                           aTAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTAc                                        <  2:2525953/150‑1 (MQ=255)
                                                                                                             aGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATATCTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACaa                                      <  1:499392/150‑1 (MQ=255)
                                                                                                              ggCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAAt                                     <  2:767348/150‑1 (MQ=255)
                                                                                                               gCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATc                                    >  1:1058856/1‑150 (MQ=255)
                                                                                                                  gCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACGGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAAc                                 >  1:1932275/1‑150 (MQ=255)
                                                                                                                  gCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAAc                                 <  2:1362806/150‑1 (MQ=255)
                                                                                                                  gCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAAc                                 <  2:852933/150‑1 (MQ=255)
                                                                                                                        aTAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTg                           <  2:108290/150‑1 (MQ=255)
                                                                                                                              gCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTc                     >  2:53606/1‑150 (MQ=255)
                                                                                                                               cACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCt                    >  1:694070/1‑150 (MQ=255)
                                                                                                                               cACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCt                    >  1:560856/1‑150 (MQ=255)
                                                                                                                                aCCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTg                   >  2:465158/1‑150 (MQ=255)
                                                                                                                                aCCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTg                   >  1:2149275/1‑150 (MQ=255)
                                                                                                                                  cAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACACTCAACCAGTTGGTTTTCTGAc                 <  2:832482/150‑1 (MQ=255)
                                                                                                                                  cAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGAc                 <  2:1827443/150‑1 (MQ=255)
                                                                                                                                  cAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGAc                 <  2:1676017/150‑1 (MQ=255)
                                                                                                                                    aTGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCa               >  1:1024280/1‑150 (MQ=255)
                                                                                                                                     tGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATCATCCAGGTACAATCAACCAGTTGGTTTTCTGACCAt              <  2:864750/150‑1 (MQ=255)
                                                                                                                                     tGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCAt              >  2:2351943/1‑150 (MQ=255)
                                                                                                                                        aCGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTaa           >  2:513043/1‑150 (MQ=255)
                                                                                                                                             aGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGa      <  1:1103965/150‑1 (MQ=255)
                                                                                                                                              ggAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGaa     <  1:2451884/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGACGCCGACGAGGGCTAATAATAATCCAGGTACAATCAACCAGTTGGTTTTCTGACCATTAAACAGAATGG  >  NC_000913/1546547‑1546841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: