BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,597,281	A→G	6.0%	pseudogene (707/3861 nt)	yneO ←	pseudogene, AidA homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,597,281	0	A	G	6.0%	451.6 / 12.6	133	pseudogene (707/3861 nt)	yneO	pseudogene, AidA homolog
Reads supporting (aligned to +/- strand): ref base A (57/68); new base G (4/4); total (61/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

CCAATGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGC  >  NC_000913/1597132‑1597428

ccaATGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGa                                                                                                                                                     <  2:861267/150‑1 (MQ=255)

  aaTGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGGTACCGGTGGCATTGCCACCGATa                                                                                                                                                   >  1:2137196/1‑150 (MQ=255)

  aaTGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATATCGGTGGCAATGCCACCGGTa                                                                                                                                                   <  1:1639942/150‑1 (MQ=255)

     ggatatTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATc                                                                                                                                                <  2:2449062/148‑1 (MQ=255)

       atatTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGt                                                                                                                                              <  2:453907/150‑1 (MQ=255)

         atTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTAt                                                                                                                                            >  1:732962/1‑150 (MQ=255)

         atTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCAATGCCACCGGTatcggaac                                                                                                                                            <  2:1420749/150‑8 (MQ=255)

         atTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATGACCGAAATATCCAGTATAGAGTCGGCGTCCCGTGCTGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCAAGGCCACCGGTatcggaac                                                                                                                                            <  2:1115057/150‑8 (MQ=255)

             tttGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTc                                                                                                                                        >  1:1208470/1‑150 (MQ=255)

              ttGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCa                                                                                                                                       <  1:168840/150‑1 (MQ=255)

              ttGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCa                                                                                                                                       <  1:189448/150‑1 (MQ=255)

                gTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGc                                                                                                                                     <  2:2426103/150‑1 (MQ=255)

                gTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGc                                                                                                                                     <  2:413162/150‑1 (MQ=255)

                gTTATTAATCACTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGACTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGc                                                                                                                                     <  2:217578/150‑1 (MQ=255)

                   attaATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGt                                                                                                                                  >  1:625347/1‑150 (MQ=255)

                   attaATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTACGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGt                                                                                                                                  >  1:567330/1‑150 (MQ=255)

                     taATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTaa                                                                                                                                <  2:640429/150‑1 (MQ=255)

                      aaTCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTaac                                                                                                                               <  1:1473035/150‑1 (MQ=255)

                            tCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAAccc                                                                                                                         >  1:1750120/1‑150 (MQ=255)

                              cATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAAccccc                                                                                                                       >  2:2116362/1‑150 (MQ=255)

                              cATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAAccccc                                                                                                                       >  1:1879349/1‑150 (MQ=255)

                                   ttGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTcg                                                                                                                  >  2:439997/1‑150 (MQ=255)

                                   ttGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTcg                                                                                                                  >  1:1907742/1‑150 (MQ=255)

                                    tGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCTCATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTcgc                                                                                                                 <  1:128223/150‑1 (MQ=255)

                                     ggAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCACCCCCCCCTcgcg                                                                                                                >  2:824798/1‑150 (MQ=255)

                                      gAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGa                                                                                                               >  1:1863435/1‑150 (MQ=255)

                                      gAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGa                                                                                                               >  2:157920/1‑150 (MQ=255)

                                      gAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGa                                                                                                               >  1:674491/1‑150 (MQ=255)

                                       aaaTATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGaa                                                                                                              >  1:314199/1‑150 (MQ=255)

                                       aaaTATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGaa                                                                                                              <  1:1518916/150‑1 (MQ=255)

                                         atatTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATa                                                                                                            >  1:1981555/1‑150 (MQ=255)

                                         atatTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATa                                                                                                            <  2:1170922/150‑1 (MQ=255)

                                           atTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGACTAGt                                                                                                          >  2:1839817/1‑150 (MQ=255)

                                           atTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGt                                                                                                          >  2:1839755/1‑150 (MQ=255)

                                              gACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGc                                                                                                       >  1:942586/1‑150 (MQ=255)

                                              gACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGc                                                                                                       <  1:9645/150‑1 (MQ=255)

                                                 tgtgCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTc                                                                                                    <  1:2418806/150‑1 (MQ=255)

                                                 tgtgCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTc                                                                                                    <  1:764392/150‑1 (MQ=255)

                                                 tgtgCCATGACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTc                                                                                                    <  1:626202/150‑1 (MQ=255)

                                                           ccGATATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTAAGTGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCt                                                                                          <  2:145972/150‑1 (MQ=255)

                                                           ccGAAAGATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGACGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCt                                                                                          <  2:2501847/150‑1 (MQ=255)

                                                            cGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCtt                                                                                         <  1:840196/150‑1 (MQ=255)

                                                             gAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTg                                                                                        <  1:1536218/150‑1 (MQ=255)

                                                             gAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTg                                                                                        <  1:1593100/150‑1 (MQ=255)

                                                                 gatCCCTTAGAGAGTCTGCGTACCGTGATGACAGAGTCGTGAGCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATACTCGTAATTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTa                                                                                    <  2:1421271/149‑1 (MQ=255)

                                                                   tCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTAcc                                                                                  <  1:503392/150‑1 (MQ=255)

                                                                   tCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTAcc                                                                                  <  1:380965/150‑1 (MQ=255)

                                                                   tCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTAcc                                                                                  <  1:546576/150‑1 (MQ=255)

                                                                     cATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCaa                                                                                >  2:2519749/1‑150 (MQ=255)

                                                                     cATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCaa                                                                                >  2:920037/1‑150 (MQ=255)

                                                                         ataGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATCGTCGCCTCCCCAGTGCCTTGATTACCAATTTg                                                                            >  1:2260874/1‑150 (MQ=255)

                                                                         ataGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAAttgg                                                                            >  1:449736/1‑148 (MQ=255)

                                                                         ataGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTg                                                                            >  2:2281258/1‑150 (MQ=255)

                                                                               tCTGCGTAACGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTc                                                                      >  1:1336826/1‑150 (MQ=255)

                                                                                     tACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGa                                                                <  2:1897656/150‑1 (MQ=255)

                                                                                     tACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGa                                                                <  1:853987/150‑1 (MQ=255)

                                                                                     tACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGa                                                                <  2:1024633/150‑1 (MQ=255)

                                                                                     tACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGa                                                                <  1:94286/150‑1 (MQ=255)

                                                                                      aCCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAg                                                               <  1:1884896/150‑1 (MQ=255)

                                                                                      aCCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAg                                                               <  1:923526/150‑1 (MQ=255)

                                                                                      aCCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAg                                                               <  1:2201913/150‑1 (MQ=255)

                                                                                      aCCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGGTCCGAGACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAg                                                               <  1:1884883/150‑1 (MQ=255)

                                                                                        cGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATggagga                                                             >  1:1325197/1‑150 (MQ=255)

                                                                                        cGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGa                                                             >  1:956181/1‑150 (MQ=255)

                                                                                        cGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGa                                                             >  1:1325207/1‑150 (MQ=255)

                                                                                                 agagTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCattatt                                                    <  2:972984/150‑1 (MQ=255)

                                                                                                 agagTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCattatt                                                    <  1:1622430/150‑1 (MQ=255)

                                                                                                  gagTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTAttt                                                   >  1:2005235/1‑150 (MQ=255)

                                                                                                      cTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTtta                                               <  2:1208470/150‑1 (MQ=255)

                                                                                                       ttGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTttat                                              >  2:1428511/1‑150 (MQ=255)

                                                                                                          aTCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAg                                           >  1:2543192/1‑150 (MQ=255)

                                                                                                           tCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGc                                          <  2:942586/150‑1 (MQ=255)

                                                                                                               gCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCCAGTTGAAATTCAATTGAGGACTCATTATTTTTTATTAGCCACt                                      >  1:1724763/1‑150 (MQ=255)

                                                                                                               gCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAGTTCAATTGAGGAATCATTATTTTTTATTAGCCACt                                      >  1:1620662/1‑150 (MQ=255)

                                                                                                               gCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAAGTTGAAATTCAATTGAGGAATCATGATTTGTTATTAGCCACt                                      >  1:2517577/1‑150 (MQ=255)

                                                                                                                 acaTTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCg                                    <  2:618874/150‑1 (MQ=255)

                                                                                                                 acaTTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCg                                    <  2:618947/150‑1 (MQ=255)

                                                                                                                       aGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCAccc                              <  1:23451/150‑1 (MQ=255)

                                                                                                                         ggTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCtt                            >  2:1750541/1‑150 (MQ=255)

                                                                                                                          gTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCttt                           >  2:2027154/1‑150 (MQ=255)

                                                                                                                          gTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCttt                           >  2:296969/1‑150 (MQ=255)

                                                                                                                              cGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCaa                       <  1:1113725/150‑1 (MQ=255)

                                                                                                                              cGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCaa                       >  1:2204660/1‑150 (MQ=255)

                                                                                                                              cGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCaa                       <  2:1490664/150‑1 (MQ=255)

                                                                                                                               gATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAAc                      <  1:1349005/150‑1 (MQ=255)

                                                                                                                                ttatcGGTGGCAATGCCACCGGTAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACg                     >  2:1972451/5‑150 (MQ=255)

                                                                                                                                ttatcGGTGGCAATGCCACCGGTAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACg                     >  2:1348181/5‑150 (MQ=255)

                                                                                                                                   tcGGTGGCAATGCCACCGGTAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACa                  >  1:655699/2‑150 (MQ=255)

                                                                                                                                   tcGGTGGCAATGCCACCGGTAATCGTATTTTCAGCCGTAACCAACCCCCCCGCGCGACTCGTCGCCTCCCCAGGTCCTTGATGACCCATGTGACAGTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGAcc                  >  1:729404/2‑149 (MQ=255)

                                                                                                                                   ccGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGAGTACCAATTTGAAATTCAATTGAGGAAGCATTCTTTTTTATTAGCCACTCGCCCCCCTTTTCAACGACa                  >  1:2257205/1‑150 (MQ=255)

                                                                                                                                        ggCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTg             <  2:2232929/150‑1 (MQ=255)

                                                                                                                                            ttGCCACCGATAATCTTATTTACAGCCGTAACCAACCCCCCCTCGCTAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATAATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCa         <  1:666252/150‑1 (MQ=255)

                                                                                                                                            ttGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCa         <  1:2536041/150‑1 (MQ=255)

                                                                                                                                            ttGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCa         <  1:2536178/150‑1 (MQ=255)

                                                                                                                                                cACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTAc     >  1:227108/1‑150 (MQ=255)

                                                                                                                                                  cctaTAATCGTATTTTCTGCCTTAACAAACCCCCCCTGTCGAATAGTCGCCTCCCAAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTg   <  1:1244094/147‑1 (MQ=255)

                                                                                                                                                  ccGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTg   <  1:920037/150‑1 (MQ=255)

                                                                                                                                                   cGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGc  <  2:1209456/150‑1 (MQ=255)

                                                                                                                                                   cGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGc  <  2:1209460/150‑1 (MQ=255)

                                                                                                                                                   cGATAATCGTATTTTATGCCGTATCCTACCCCCCATCTATTATAGTCGGCTCGCCATTTCCTTGATTACCAATTTGAAATTAATTTTTTGATTGATTATTTTTTATTATCCTCTCGACACCCTTTTCTACGACAACCTGTCCATTACTGc  <  2:1604249/150‑1 (MQ=255)

CCAATGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGC  >  NC_000913/1597132‑1597428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`