Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,629,435 G→A 5.1% intergenic (+17/+18) ydfZ → / ← ydfI selenoprotein, function unknown/putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,629,4350GA 5.1% 682.1 / 17.2 195intergenic (+17/+18)ydfZ/ydfIselenoprotein, function unknown/putative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (121/64);  new base A (5/5);  total (126/69)
Fisher's exact test for biased strand distribution p-value = 3.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01

Only 100 of 209 total aligned reads displayed.

ACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGC  >  NC_000913/1629287‑1629581
                                                                                                                                                    |                                                                                                                                                  
acTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGa                                                                                                                                                   >  1:1778850/1‑150 (MQ=255)
acTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACGGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGa                                                                                                                                                   >  1:1294196/1‑150 (MQ=255)
  tGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGACTGCCGCCGCTg                                                                                                                                                 >  2:1426313/1‑150 (MQ=255)
   ggCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATgg                                                                                                                                                <  1:235851/150‑1 (MQ=255)
    gCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATggc                                                                                                                                               <  1:1839368/150‑1 (MQ=255)
    gCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATggc                                                                                                                                               <  1:433397/150‑1 (MQ=255)
      aaGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATggcgg                                                                                                                                             <  2:425266/150‑1 (MQ=255)
        gATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCATCGGCGGCa                                                                                                                                           <  2:229744/150‑1 (MQ=255)
         aTCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCAt                                                                                                                                          >  2:1225426/1‑150 (MQ=255)
          tCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCAtt                                                                                                                                         >  1:2320201/1‑150 (MQ=255)
          tCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCATCGGCGGCAtt                                                                                                                                         <  1:2518471/150‑1 (MQ=255)
            cTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGc                                                                                                                                       >  1:268344/1‑150 (MQ=255)
                   ttGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTa                                                                                                                                >  1:2525929/1‑150 (MQ=255)
                      aTACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACtt                                                                                                                             >  1:2249710/1‑150 (MQ=255)
                       tACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTACTTCa                                                                                                                           <  1:1986698/150‑1 (MQ=255)
                          tGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACg                                                                                                                         >  1:179341/1‑150 (MQ=255)
                          tGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACg                                                                                                                         >  1:698844/1‑150 (MQ=255)
                             aGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGaa                                                                                                                      <  1:1005858/150‑1 (MQ=255)
                                tCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAatat                                                                                                                   >  1:1135012/1‑150 (MQ=255)
                                        cTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCAc                                                                                                           >  1:1743305/1‑150 (MQ=255)
                                        cTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCAc                                                                                                           >  1:1917504/1‑150 (MQ=255)
                                         tGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTAAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACg                                                                                                          >  1:1282758/1‑150 (MQ=255)
                                            gCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCCTTGCTTTTTTACTTCACGGAATATTTTGCCACGGTc                                                                                                       >  2:2003929/1‑150 (MQ=255)
                                                   cgccgcGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTcg                                                                                                >  2:77083/1‑150 (MQ=255)
                                                    gccgcGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTcgc                                                                                               >  2:2166254/1‑150 (MQ=255)
                                                    gccgcGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTcgc                                                                                               <  2:1773254/150‑1 (MQ=255)
                                                     ccgcGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTcgcg                                                                                              >  1:2251805/1‑150 (MQ=255)
                                                        cgGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGGACCACTGGACCTGATTAGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCa                                                                                           <  1:628327/150‑1 (MQ=255)
                                                           aaaaaCCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGcccagc                                                                                        >  1:488831/1‑146 (MQ=255)
                                                             aaaCCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATgcgc                                                                                      <  1:1772483/150‑1 (MQ=255)
                                                                    aGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATaaa                                                                               <  2:1135012/150‑1 (MQ=255)
                                                                    aGTTGTTGAAGGGTGTGAAGAGACACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTACGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATaaa                                                                               <  1:2216035/150‑1 (MQ=255)
                                                                         ttgAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATaaataaaa                                                                          >  2:1322511/1‑145 (MQ=255)
                                                                         ttgAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTAGGGCCCTGCGCGAAAAAAGACaa                                                                          >  1:1397608/1‑150 (MQ=255)
                                                                             aGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTAc                                                                      <  1:1473500/150‑1 (MQ=255)
                                                                             aGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTAc                                                                      >  2:1559665/1‑150 (MQ=255)
                                                                               gTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGt                                                                    >  2:1801303/1‑150 (MQ=255)
                                                                               gTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAAAGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGt                                                                    >  2:1633968/1‑150 (MQ=255)
                                                                                ttGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGtt                                                                   <  1:2508241/150‑1 (MQ=255)
                                                                                   tgAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTcc                                                                <  2:2525929/150‑1 (MQ=255)
                                                                                   tgAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCGAATAAAGACAAGTACGTTTcc                                                                >  1:1867054/1‑150 (MQ=255)
                                                                                     aaGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGt                                                              >  2:835937/1‑150 (MQ=255)
                                                                                     aaGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGt                                                              >  2:1921609/1‑150 (MQ=255)
                                                                                     aaGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGt                                                              >  2:2277736/1‑150 (MQ=255)
                                                                                      agagAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTAGCGCCATGCGGTAATTAATAAAAGTACGTTTACGTc                                                             >  1:1201506/1‑150 (MQ=255)
                                                                                       gagaAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCa                                                            <  2:455275/150‑1 (MQ=255)
                                                                                       gagaAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCa                                                            >  1:1402905/1‑150 (MQ=255)
                                                                                       gagaAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTAGCGCCATGCGCTAATAAACAAAATTAAGTTTCCGTCa                                                            >  2:508535/1‑150 (MQ=255)
                                                                                          aaaCTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCActc                                                         >  2:1026385/1‑150 (MQ=255)
                                                                                            aCTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCAATGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCAAGCGCTAATAAAGACAAGTACGTTTCCGacacacgt                                                       >  2:1842007/1‑146 (MQ=255)
                                                                                                gCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCAAGCGCGAAAAAAGACAACTACCGTTCCGTCACCCTTGCAg                                                   >  1:2182191/1‑150 (MQ=255)
                                                                                                gCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCCCCCTGCGCGAATAAATACAAGAAAGTTTCCGTCACACTTGCAg                                                   >  1:2406833/1‑150 (MQ=255)
                                                                                                gCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTAGCGCCATGCGCTAATAAAGACAAGTAACTTTCCGTCACACTTGCAg                                                   >  1:1091836/1‑150 (MQ=255)
                                                                                                 caccacTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGt                                                  <  2:124532/150‑1 (MQ=255)
                                                                                                  accacTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCGAATAAAGACAACTACGTTTCCGGCACTCATGCAgga                                                 >  1:850234/1‑148 (MQ=255)
                                                                                                   ccacTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaa                                                <  2:2122024/150‑1 (MQ=255)
                                                                                                   ccacTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaa                                                <  2:2454372/150‑1 (MQ=255)
                                                                                                    cacTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaa                                               <  2:2243860/150‑1 (MQ=255)
                                                                                                     acTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaac                                              >  2:2569655/1‑150 (MQ=255)
                                                                                                      cTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAATACAAGTACGTTTCCGTCACTCATGCAGTaaaca                                             >  1:1726609/1‑150 (MQ=255)
                                                                                                      cTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGAAAAGTAAGTTTACGTCACTCGTTCAGTaaaaa                                             >  1:956579/1‑148 (MQ=255)
                                                                                                       tGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaacaa                                            <  2:914744/150‑1 (MQ=255)
                                                                                                       tGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaacaa                                            <  2:1749971/150‑1 (MQ=255)
                                                                                                        ggACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaacaaa                                           >  2:859353/1‑150 (MQ=255)
                                                                                                        ggACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaacaaa                                           <  1:789028/150‑1 (MQ=255)
                                                                                                        ggACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaacaaa                                           <  1:860381/150‑1 (MQ=255)
                                                                                                        ggACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAAAAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaacaaa                                           >  2:2179167/1‑150 (MQ=255)
                                                                                                        ggACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCAAGCGCTAATAAAGACAACTACGTTTCCGTCACTCTAGCAGTaaacaaa                                           >  2:2493735/1‑150 (MQ=255)
                                                                                                            cTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTAt                                       >  2:378606/1‑150 (MQ=255)
                                                                                                               aTTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGt                                    <  1:2168037/150‑1 (MQ=255)
                                                                                                                ccatcggcGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTACGTCACACTTGCAGTAAACAAACTATTGTc                                   >  2:2543843/8‑150 (MQ=255)
                                                                                                                   gTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCCTTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCGTTCGCGCCCGGCGCGAATAAAGACAAGTACGGTTCCGGCACGCATGCAGGAAACCAACAAATGTCTgg                                >  1:323864/1‑150 (MQ=255)
                                                                                                                   gTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTgg                                <  2:1388755/150‑1 (MQ=255)
                                                                                                                   gTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCAGGCGCGAATAAAGACAAGTAAGTTTCCGTCACTCTTGCAGTAAACAAAatatattatgg                                >  1:1855343/1‑143 (MQ=255)
                                                                                                                   gTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCGGTCGCGCCCGGCGCGAAAAAAGACAAGTACGTTTCCGGCACGCGTGCAGTAAACCAACAATTGTCTgg                                >  1:812618/1‑150 (MQ=255)
                                                                                                                    tctcGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGc                               >  2:303140/1‑150 (MQ=255)
                                                                                                                    tctcGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCGAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACAATTGTCTGGc                               >  2:44710/1‑150 (MQ=255)
                                                                                                                        ggCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAat                           >  2:1518546/1‑150 (MQ=255)
                                                                                                                         gCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAatc                          <  2:247981/150‑1 (MQ=255)
                                                                                                                          cATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCCTGCGCGAATAAAGACAAGTAAGTTTCCGTCACACGTGCAGTAAAAAAACTAATTTATGGCAAatca                         >  1:2281400/1‑150 (MQ=255)
                                                                                                                           aTGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAATACAAGTAAGTTTGCGTCACTCTTGCAGTAAACAAACTATTTTCTGGCAAatcat                        >  2:1968829/1‑150 (MQ=255)
                                                                                                                           aTGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCGAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACAAATGTCTGGCAAatcat                        >  2:1829249/1‑150 (MQ=255)
                                                                                                                            tGATCTAAGCGTGTGATTGCCGCGGATGGCGGCATTTCTTTTTTACTTCACTGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATATAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAatcatc                       <  1:1610654/150‑1 (MQ=255)
                                                                                                                            tGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCCTGCGCTAATAAAGACAAATAAGTTTACCTCACACATGCAGTAAAAAAAATATTTTATGGCAAaacata                       >  1:2217602/1‑149 (MQ=255)
                                                                                                                             gAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTAGCGCCATGCGCGAATAAAGAAAAGTACGTTTCCGGCACTCTTTCAGTAAACAAAAAATTGTATGGCAAAtaatca                      >  1:1494220/1‑150 (MQ=255)
                                                                                                                              aaCTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTAGCGCCATGCCCAAATAAATACAAGTACCTTTACGTCACACATGCAGTAAACAAACTATTGTCTGGCaaaaactcac                     >  2:1757272/1‑143 (MQ=255)
                                                                                                                              aaCTAAGCGTGTGAAGGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCAc                     <  2:1719643/150‑1 (MQ=255)
                                                                                                                                 tAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCCAGCGCGAAAAAAAAAAACTAAGTTTCCGTCCCACATGCAGGAAACAAAAAAATTTCTGGCAAAACAAAACCaa                  >  1:1861138/1‑150 (MQ=255)
                                                                                                                                 tAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCaa                  >  2:343523/1‑150 (MQ=255)
                                                                                                                                 tAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTAGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAAAAAACAATTGTCTGGCAAATCATCACCaa                  >  2:26016/1‑150 (MQ=255)
                                                                                                                                       aagcaaTGCCGCCATCGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCg            >  2:1041607/5‑150 (MQ=255)
                                                                                                                                        gtgAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGc           <  1:975592/150‑1 (MQ=255)
                                                                                                                                          gAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCt         <  2:284607/150‑1 (MQ=255)
                                                                                                                                           aaTGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCGTTCGCGCCCAGCGCGAATAAAGACAAGAAACATTCCGTCACGCATGCAGTAAACAAAAAAATGTCTGGCCAAACAAAAACAAAAATAGccat        >  1:1260104/1‑148 (MQ=255)
                                                                                                                                             tGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTaa      <  2:2249716/150‑1 (MQ=255)
                                                                                                                                               ccgccgATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATc    <  2:731558/150‑1 (MQ=255)
                                                                                                                                               ccgccgATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATc    <  1:2471611/150‑1 (MQ=255)
                                                                                                                                                cgccgATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCg   >  2:2544772/1‑150 (MQ=255)
                                                                                                                                                cgccgATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCg   >  2:2249096/1‑150 (MQ=255)
                                                                                                                                                cgccgATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCGAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCg   >  2:2215142/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGC  >  NC_000913/1629287‑1629581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: