Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,939,994 A→G 5.2% L67S (TTA→TCA)  lpxM ← myristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,939,9940AG 5.2% 399.6 / 8.7 116L67S (TTA→TCA) lpxMmyristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (58/51);  new base G (4/2);  total (62/54)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 121 total aligned reads displayed.

CGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCTCCCCAGTAGCGCGGGTGGCGAAAG  >  NC_000913/1939855‑1940143
                                                                                                                                           |                                                                                                                                                     
cgGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTGACGCacgacg                                                                                                                                             >  2:1710652/1‑150 (MQ=255)
cgGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTGACGCacgacg                                                                                                                                             >  2:849585/1‑150 (MQ=255)
 ggCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGAGTGATTAACGCACGACgg                                                                                                                                            >  1:2319215/1‑150 (MQ=255)
  gCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGc                                                                                                                                           >  2:241584/1‑150 (MQ=255)
     gAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCgcg                                                                                                                                        >  2:2160352/1‑148 (MQ=255)
      aaTTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTCACGCACGACGGCGTGa                                                                                                                                       <  2:172493/150‑1 (MQ=255)
      aaTTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGa                                                                                                                                       >  2:2217612/1‑150 (MQ=255)
      aaTTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGa                                                                                                                                       <  1:1166133/150‑1 (MQ=255)
       tcccattcCGGCCCGCGTATTGCCAACTCAGCCAACATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAg                                                                                                                                      >  2:2401496/7‑150 (MQ=255)
        ttttCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGc                                                                                                                                     >  1:1053433/1‑150 (MQ=255)
          ttCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCtt                                                                                                                                   <  2:2442379/150‑1 (MQ=255)
            ctcCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCtttt                                                                                                                                 <  2:1579560/150‑1 (MQ=255)
            ctcCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCtttt                                                                                                                                 <  2:1086387/150‑1 (MQ=255)
            ctcCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCtttt                                                                                                                                 <  2:1086425/150‑1 (MQ=255)
             tcCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCttttt                                                                                                                                <  1:1371363/150‑1 (MQ=255)
             tcCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCttttt                                                                                                                                <  1:2494025/150‑1 (MQ=255)
              ccGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTc                                                                                                                               >  1:1491996/1‑150 (MQ=255)
              ccGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTc                                                                                                                               <  2:261782/150‑1 (MQ=255)
                 gCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCa                                                                                                                            <  2:1778993/150‑1 (MQ=255)
                 gCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCa                                                                                                                            >  2:495144/1‑150 (MQ=255)
                 gCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCa                                                                                                                            <  2:2083174/150‑1 (MQ=255)
                    cgcgTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAgtc                                                                                                                         >  1:533950/1‑150 (MQ=255)
                    cgcgTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAgtc                                                                                                                         >  1:533963/1‑150 (MQ=255)
                            gCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTGACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCa                                                                                                                 >  1:2075571/1‑150 (MQ=255)
                            gCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTGACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCa                                                                                                                 >  1:2075574/1‑150 (MQ=255)
                            gCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCa                                                                                                                 >  2:1662068/1‑150 (MQ=255)
                            gCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCa                                                                                                                 >  2:1662161/1‑150 (MQ=255)
                              cAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCaaa                                                                                                               <  2:135301/150‑1 (MQ=255)
                              cAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCaaa                                                                                                               <  2:1500053/150‑1 (MQ=255)
                              cAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCaaa                                                                                                               <  2:1936638/150‑1 (MQ=255)
                              cAACTCAGCCATCATTGCCATCGCGTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCaaa                                                                                                               <  1:800329/150‑1 (MQ=255)
                                  tCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGt                                                                                                           >  2:2116021/1‑150 (MQ=255)
                                                cATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAg                                                                                             >  1:190823/1‑150 (MQ=255)
                                                cATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAg                                                                                             <  2:686799/150‑1 (MQ=255)
                                                  tCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGaa                                                                                           >  1:2133062/1‑150 (MQ=255)
                                                   cGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAAt                                                                                          <  2:2253464/150‑1 (MQ=255)
                                                     cTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATgg                                                                                        >  1:733032/1‑150 (MQ=255)
                                                     cTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATgg                                                                                        >  1:1449953/1‑150 (MQ=255)
                                                       tGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGa                                                                                      <  1:2401496/150‑1 (MQ=255)
                                                           gcgcGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCAc                                                                                  <  1:867877/150‑1 (MQ=255)
                                                                   gCAAACATCTCATCAACACTCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACttt                                                                          <  2:1327958/150‑1 (MQ=255)
                                                                   gCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACttt                                                                          >  1:2336820/1‑150 (MQ=255)
                                                                     aaaCATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTgg                                                                        >  2:2061938/1‑150 (MQ=255)
                                                                     aaaCATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTgg                                                                        >  2:1995687/1‑150 (MQ=255)
                                                                     aaaCATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTgg                                                                        >  2:96461/1‑150 (MQ=255)
                                                                     aaaCATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTgg                                                                        >  2:815776/1‑150 (MQ=255)
                                                                     aaaCATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTgg                                                                        >  1:2390452/1‑150 (MQ=255)
                                                                     aaaCATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTgg                                                                        >  2:2561747/1‑150 (MQ=255)
                                                                     aaaCATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTgg                                                                        >  2:480357/1‑150 (MQ=255)
                                                                     aaaCATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTgg                                                                        >  2:539943/1‑150 (MQ=255)
                                                                     aaaCATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTgg                                                                        >  2:539972/1‑150 (MQ=255)
                                                                     aaaCATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTgg                                                                        >  2:539974/1‑150 (MQ=255)
                                                                     aaaCATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTgg                                                                        >  2:815441/1‑150 (MQ=255)
                                                                      aaCATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTggc                                                                       <  1:1938467/150‑1 (MQ=255)
                                                                         aTCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTggcggc                                                                    <  1:726158/150‑1 (MQ=255)
                                                                         aTCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTggcggc                                                                    <  1:539392/150‑1 (MQ=255)
                                                                         aTCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTggcggc                                                                    <  2:2446316/150‑1 (MQ=255)
                                                                           ctcATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGt                                                                  <  2:2193861/150‑1 (MQ=255)
                                                                           ctcATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGt                                                                  <  2:588485/150‑1 (MQ=255)
                                                                            tcatcaACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGtt                                                                 >  1:2491118/1‑150 (MQ=255)
                                                                                 aacaaTCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGc                                                            >  2:2195079/1‑150 (MQ=255)
                                                                                   caaTCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGa                                                          <  1:1458719/150‑1 (MQ=255)
                                                                                       cGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATAcc                                                      <  2:2100556/150‑1 (MQ=255)
                                                                                        gCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATAccc                                                     <  2:689982/150‑1 (MQ=255)
                                                                                        gCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATAccc                                                     <  2:126780/150‑1 (MQ=255)
                                                                                                   aGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCgctgc                                          <  2:235364/150‑1 (MQ=255)
                                                                                                    gCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGCGAGCTATTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCgctgct                                         <  1:2160352/150‑1 (MQ=255)
                                                                                                     cTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTa                                        <  1:2453500/150‑1 (MQ=255)
                                                                                                     cTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTa                                        >  2:1124702/1‑150 (MQ=255)
                                                                                                      ttCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTAc                                       >  2:2405652/1‑150 (MQ=255)
                                                                                                       tCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCCGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACg                                      <  1:147813/150‑1 (MQ=255)
                                                                                                       tCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACg                                      <  1:1480808/150‑1 (MQ=255)
                                                                                                       tCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACg                                      <  1:1480806/150‑1 (MQ=255)
                                                                                                            aCGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAg                                 <  1:2224395/150‑1 (MQ=255)
                                                                                                             cGTTCTGGAAATCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGc                                <  1:555090/150‑1 (MQ=255)
                                                                                                                tCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCAt                             >  1:903009/1‑150 (MQ=255)
                                                                                                                tCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCAt                             >  1:903069/1‑150 (MQ=255)
                                                                                                                tCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCAt                             <  1:1781556/150‑1 (MQ=255)
                                                                                                                  tGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGc                           >  1:1590548/1‑150 (MQ=255)
                                                                                                                   ggAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCt                          >  2:174894/1‑150 (MQ=255)
                                                                                                                   ggAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCt                          >  2:455513/1‑150 (MQ=255)
                                                                                                                   ggAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCt                          >  1:179398/1‑150 (MQ=255)
                                                                                                                    gAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCTc                         >  2:1686546/1‑150 (MQ=255)
                                                                                                                    gAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCTc                         >  1:1602500/1‑150 (MQ=255)
                                                                                                                    gAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCTc                         >  1:1602496/1‑150 (MQ=255)
                                                                                                                     aaaGCAGAGCGACAGATTGATTGACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCTcc                        <  1:1710652/150‑1 (MQ=255)
                                                                                                                     aaaGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCTcc                        >  2:306193/1‑150 (MQ=255)
                                                                                                                     aaaGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCTcc                        >  2:306205/1‑150 (MQ=255)
                                                                                                                        gCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCTCCCCa                     <  2:581890/150‑1 (MQ=255)
                                                                                                                         cAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCTCCCCAg                    >  2:1126554/1‑150 (MQ=255)
                                                                                                                         cAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCTCCCCAg                    >  2:767780/1‑150 (MQ=255)
                                                                                                                                    attgattAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCTCCCCAGTAGCGCGGGTg         >  2:1960969/1‑150 (MQ=255)
                                                                                                                                    attgattAACGCACGACGGAGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAAAGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCAAGCTCCCCAGTAGCGCGGGTg         >  1:2584748/1‑150 (MQ=255)
                                                                                                                                     ttgattAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCTCCCCAGTAGCGCGGGTgg        <  2:2508477/150‑1 (MQ=255)
                                                                                                                                         ttAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCTCCCCAGTAGCGCGGGTGGCGaa    <  1:1056294/150‑1 (MQ=255)
                                                                                                                                           aaCGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGATCTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCTCCCCAGTAGCGCGGGTGGCGAAAg  <  1:2112074/150‑1 (MQ=255)
                                                                                                                                           aaCGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCTCCCCAGTAGCGCGGGTGGCGTAAg  <  1:295398/150‑1 (MQ=255)
                                                                                                                                           aaCGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCTCCCCAGTAGCGCGGGTGGCGAAAg  <  1:1668397/150‑1 (MQ=255)
                                                                                                                                           aaCGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCTCCCCAGTAGCGCGGGTGGCGAAAg  <  1:1812485/150‑1 (MQ=255)
                                                                                                                                           aaCGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCTCCCCAGTAGCGCGGGTGGCGAAAg  <  1:32297/150‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                     
CGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAAACGTCCCAGCCGTGCCAGAATGGGATCACGGAACTTTGGCGGCGTTAAAGCGATACCCGCCATCGCTGCTACGCCCAGCCATGCTCCCCAGTAGCGCGGGTGGCGAAAG  >  NC_000913/1939855‑1940143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: