Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,068,408 T→A 7.3% pseudogene (12/159 nt) yoeH ← pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,068,4080TA 7.3% 353.1 / 10.3 107pseudogene (12/159 nt)yoeHpseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base T (49/49);  new base A (6/3);  total (55/52)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

GACCTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACAA  >  NC_000913/2068259‑2068553
                                                                                                                                                     |                                                                                                                                                 
gACCTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTAt                                                                                                                                                   <  2:1886925/150‑1 (MQ=255)
       tactgataTGAGTCTTACCGCATATACGTTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAaga                                                                                                                                            <  2:2522396/150‑1 (MQ=255)
         ctgataTGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGt                                                                                                                                          <  1:2073393/150‑1 (MQ=255)
          tgataTGAGTCTTACCTCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTg                                                                                                                                         <  2:2528805/150‑1 (MQ=255)
          tgataTGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTAGATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTg                                                                                                                                         <  1:733789/150‑1 (MQ=255)
          tgataTGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTg                                                                                                                                         <  1:830509/150‑1 (MQ=255)
          tgataTGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTg                                                                                                                                         <  2:924649/150‑1 (MQ=255)
          tgataTGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTg                                                                                                                                         <  2:411759/150‑1 (MQ=255)
             tatGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCgg                                                                                                                                      <  2:2061703/150‑1 (MQ=255)
              atGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCGGt                                                                                                                                     <  2:741441/150‑1 (MQ=255)
                     ttACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGa                                                                                                                              >  1:921139/1‑150 (MQ=255)
                      tACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGAt                                                                                                                             <  2:437397/150‑1 (MQ=255)
                        ccGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATAc                                                                                                                           >  2:1052081/1‑150 (MQ=255)
                        ccGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATAc                                                                                                                           >  1:1536336/1‑150 (MQ=255)
                        ccGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATAc                                                                                                                           >  2:1565486/1‑150 (MQ=255)
                           cATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAAc                                                                                                                        <  2:2244834/150‑1 (MQ=255)
                            atatACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCGGTCCGTGGaaaacgga                                                                                                                       <  2:2063428/150‑8 (MQ=38)
                                   gTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGAcc                                                                                                                <  2:1317654/150‑1 (MQ=255)
                                     cAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGa                                                                                                              <  1:406309/150‑1 (MQ=255)
                                       aTTTCAGCAATTAATTACATTATCCCCGCCAAAGTATTGGTCATCACAATGATGGTACCTTCTTTCCGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACt                                                                                                            <  2:2025531/150‑1 (MQ=255)
                                       aTTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACt                                                                                                            >  2:139884/1‑150 (MQ=255)
                                       aTTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACt                                                                                                            <  1:2304955/150‑1 (MQ=255)
                                        tttCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTg                                                                                                           >  1:2534204/1‑150 (MQ=255)
                                          tCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGac                                                                                                         <  1:1090946/150‑1 (MQ=255)
                                            aGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGacac                                                                                                       >  1:971820/1‑150 (MQ=255)
                                            aGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGacac                                                                                                       >  1:1105426/1‑150 (MQ=255)
                                               aattaattACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGacac                                                                                                       <  2:898372/147‑1 (MQ=255)
                                               aattaattACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAg                                                                                                    >  1:2022660/1‑150 (MQ=255)
                                                 ttaattaCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTc                                                                                                  <  2:1645721/150‑1 (MQ=255)
                                                    attaCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCaga                                                                                               >  1:512140/1‑150 (MQ=255)
                                                    attaCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCaga                                                                                               <  2:2183382/150‑1 (MQ=255)
                                                       aCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGCTACAACCAGAGACCGACTGACACGCGTCAGAGGa                                                                                            >  2:567147/1‑150 (MQ=255)
                                                       aCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGa                                                                                            >  2:1852410/1‑150 (MQ=255)
                                                            aTCCACGCCAAATTATTTTTAATCAAATTTATGGTACCTTCTTTCAGAAACCATTTTTTCAACTCCGTTTTCCACGGACCGAACTCTTTTGTCAAGTGTGCGTTCCGTGTATACAACCTGAGACCGACTGACACTAGTCAGTGGAAacga                                                                                       <  1:499223/150‑1 (MQ=255)
                                                            aTCCACGCCAAAGTATTTGTCATCAAAATGATGGTACCTTATTTCAGACACAATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGTGGAAacga                                                                                       <  1:53115/150‑1 (MQ=255)
                                                             tCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAacgac                                                                                      >  1:1721462/1‑150 (MQ=255)
                                                             tCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAacgac                                                                                      >  1:1822175/1‑150 (MQ=255)
                                                             tCCACGCCAAAGTAATTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCAACGGACCGCACTCTTATCTCAACAGTGCGGTCCGGGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAacgac                                                                                      >  1:1709248/1‑150 (MQ=255)
                                                              ccACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAacgacg                                                                                     <  2:946893/150‑1 (MQ=255)
                                                               cACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACgg                                                                                    <  2:10051/150‑1 (MQ=255)
                                                               cACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACgg                                                                                    >  1:668951/1‑150 (MQ=255)
                                                               cACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACgg                                                                                    <  2:636296/150‑1 (MQ=255)
                                                               cACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACgg                                                                                    <  2:2579735/150‑1 (MQ=255)
                                                               cACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACgg                                                                                    <  2:1306001/150‑1 (MQ=255)
                                                                  gCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCCGAGACCGACTGACACGAGTCAGAGGAAACGACGGata                                                                                 >  1:1900425/1‑150 (MQ=255)
                                                                  gCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGata                                                                                 >  2:1527855/1‑150 (MQ=255)
                                                                    cAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATg                                                                               >  2:1894679/1‑150 (MQ=255)
                                                                      atgtttttGTCATCACAATGTTGGTACATTCTTTCAGACACCTTTTTTACAACTCCGTTTTCCACGGACCGCACTCTTATTCCAAGTGTGCGTTCCGTGTATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGtt                                                                             <  1:1301741/145‑1 (MQ=255)
                                                                      aaGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGtt                                                                             <  2:1332208/150‑1 (MQ=255)
                                                                      aaGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGtt                                                                             >  1:347533/1‑150 (MQ=255)
                                                                       aGTATTTGTCATCACACTGTTGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGTGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTc                                                                            <  1:1474842/150‑1 (MQ=255)
                                                                           tttGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTc                                                                        <  1:139884/150‑1 (MQ=255)
                                                                            ttGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCg                                                                       >  2:90573/1‑150 (MQ=255)
                                                                            ttGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCg                                                                       >  2:1147197/1‑150 (MQ=255)
                                                                            ttGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCg                                                                       >  2:665468/1‑150 (MQ=255)
                                                                                  tcaCAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACTGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCTACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAat                                                                 <  1:2464504/150‑1 (MQ=255)
                                                                                  tcaCAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGTCCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATAAAACCAGAGACCGACTGACACGAGTCAGAGGAAAAGACGGATATGTTAAGTCGTAAAat                                                                 >  1:582126/1‑150 (MQ=255)
                                                                                  tcaCAATGATGGTACCTTCATTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCCCACACTTAAGTCAAGAGTGCGGTCCGTGGATAAAAACAGAGAACGACAGACACGAGTCAGAGGAAAAGACGGATATGTTGAGTCGTAAAat                                                                 >  1:963354/1‑150 (MQ=255)
                                                                                   cacaATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAata                                                                <  1:2544293/150‑1 (MQ=255)
                                                                                   cacaATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAata                                                                <  2:361151/150‑1 (MQ=255)
                                                                                     caATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAAtatc                                                              <  2:848357/150‑1 (MQ=255)
                                                                                     caATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAAtatc                                                              <  2:1058459/150‑1 (MQ=255)
                                                                                      aaTGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAAtatct                                                             >  2:397365/1‑150 (MQ=255)
                                                                                      aaTGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAAtatct                                                             >  2:397363/1‑150 (MQ=255)
                                                                                      aaTGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACACTTAAGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACAGACACGAGTCAGAGGAAAAGACCGAAATGTTCAGTCGTAAAatatat                                                             >  1:1080384/1‑148 (MQ=255)
                                                                                        tgatgGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAAtatctat                                                           <  2:1556307/150‑1 (MQ=255)
                                                                                             gTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTTCGGTCCGTGGATACAAACAGAGACCGACAGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCaaaa                                                      >  1:86678/1‑150 (MQ=255)
                                                                                              tACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCaaaaa                                                     >  2:499653/1‑150 (MQ=255)
                                                                                              tACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCaaaaa                                                     >  2:2128661/1‑150 (MQ=255)
                                                                                                  ttcttTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACAt                                                 <  2:1740458/150‑1 (MQ=255)
                                                                                                   tcttTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATg                                                <  2:1736560/150‑1 (MQ=255)
                                                                                                        cAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTaa                                           <  1:2060870/150‑1 (MQ=255)
                                                                                                         aGACACCATTTTTTCAAATCCGTTTTCCACCGACCGCACTAATAAGTAAATAGTGCGGTCCGTGGATACAAAACAAGACCGACTTAAACCAGAAAGATGAAAAGACGGATATTTTCAGTAGTAAAATATATATCAAAAAAAATGATTAAg                                          >  1:1439322/1‑150 (MQ=255)
                                                                                                             acCATTTTTTCAACTCCGTTTTCCACGGGCCCCACTCTTATGTCAAGAGTGCGGGCCGGGGATACAACCACAGACCGACAGACACGCGTCAGAGGCAACGACCGATATGTTCAGTCGTAAAAAATAAATAAAAAAACAAGATTAAGGTCa                                      >  1:57368/1‑150 (MQ=255)
                                                                                                                aTTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCaaaa                                   >  2:2388512/1‑150 (MQ=255)
                                                                                                                    tttCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGt                               >  2:949407/1‑150 (MQ=255)
                                                                                                                     ttCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGtt                              <  2:2227852/150‑1 (MQ=255)
                                                                                                                     ttCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGtt                              <  1:57726/150‑1 (MQ=255)
                                                                                                                          cTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGata                         >  2:2357540/1‑150 (MQ=255)
                                                                                                                           tCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCCGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTAGCAAAAAACATGATTAAGGTCAAAAATGTTTGatat                        >  2:178191/1‑150 (MQ=255)
                                                                                                                           tCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAATGTCAAAAAAGTTTGatat                        >  2:2343219/1‑150 (MQ=255)
                                                                                                                           tCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATTTTTGatat                        >  2:840793/1‑150 (MQ=255)
                                                                                                                           tCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGatat                        >  2:851467/1‑150 (MQ=255)
                                                                                                                           tCCGTTTTCCACGGACCGCACTCGTATGTCAAGAGTGCGGGCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGatat                        >  2:178156/1‑150 (MQ=255)
                                                                                                                             cGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATAttt                      >  2:389659/1‑150 (MQ=255)
                                                                                                                             cGTTTTCCACGGACCCCACTCTAATCACAACAGTGCGGTCCGAGGATACAACCACACACCCACACACAAGACACAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCAAACAAAAAACATGATAAACGTCAAAAATCATTGATAatt                      <  2:1882582/150‑1 (MQ=255)
                                                                                                                                     aCGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACTAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTa              <  2:1439322/150‑1 (MQ=255)
                                                                                                                                     aCGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTa              >  2:979443/1‑150 (MQ=255)
                                                                                                                                        gACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGCCTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGa           <  1:1975328/150‑1 (MQ=255)
                                                                                                                                         aCCGCACTCTTGACATAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGCTTAAGGTCAAAACTGTGTGATCTTTACAATTTATGaa          >  2:604478/1‑150 (MQ=255)
                                                                                                                                         aCCGCACTCTTGACATAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGaa          >  2:2305260/1‑150 (MQ=255)
                                                                                                                                         aCCGCACTCTTGACATAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGaa          >  2:1994199/1‑150 (MQ=255)
                                                                                                                                         aCCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGaa          >  1:185819/1‑150 (MQ=255)
                                                                                                                                         aCCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTAGGaa          <  2:351913/150‑1 (MQ=255)
                                                                                                                                           cGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGa        >  2:1655015/1‑150 (MQ=255)
                                                                                                                                                 ctTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACaa  >  1:712753/1‑150 (MQ=255)
                                                                                                                                                 ctTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACaa  >  1:2010269/1‑150 (MQ=255)
                                                                                                                                                 ctTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACaa  >  1:1134250/1‑150 (MQ=255)
                                                                                                                                                 ctTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACaa  >  1:1086005/1‑150 (MQ=255)
                                                                                                                                                 ctTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACaa  >  1:1029058/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GACCTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACAA  >  NC_000913/2068259‑2068553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: