Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,154,847 A→G 5.7% S278G (AGT→GGT)  mdtA → multidrug efflux system, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,154,8470AG 5.7% 478.9 / 12.8 140S278G (AGT→GGT) mdtAmultidrug efflux system, subunit A
Reads supporting (aligned to +/- strand):  ref base A (56/76);  new base G (4/4);  total (60/80)
Fisher's exact test for biased strand distribution p-value = 7.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 144 total aligned reads displayed.

TCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACGCCGTAGTGATCCCAACAG  >  NC_000913/2154698‑2154994
                                                                                                                                                     |                                                                                                                                                   
tCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTaa                                                                                                                                                     <  1:1056981/150‑1 (MQ=255)
tCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTaa                                                                                                                                                     <  2:2404177/150‑1 (MQ=255)
tCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTaa                                                                                                                                                     >  1:78661/1‑150 (MQ=255)
tCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTaa                                                                                                                                                     <  1:1707754/150‑1 (MQ=255)
  aCCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGt                                                                                                                                                   >  1:686688/1‑150 (MQ=255)
  aCCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGt                                                                                                                                                   >  1:625607/1‑150 (MQ=255)
      aGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCATCTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAg                                                                                                                                               <  2:1896589/150‑1 (MQ=255)
      aGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAg                                                                                                                                               <  2:2454750/150‑1 (MQ=255)
          gCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATaa                                                                                                                                           <  2:958996/150‑1 (MQ=255)
          gCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATaa                                                                                                                                           <  1:107645/150‑1 (MQ=255)
           cATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAAc                                                                                                                                          >  1:39984/1‑150 (MQ=255)
           cATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAAc                                                                                                                                          >  1:1352328/1‑150 (MQ=255)
            aTCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAAcc                                                                                                                                         <  1:2418272/150‑1 (MQ=255)
              ccTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCaa                                                                                                                                       <  1:1860212/150‑1 (MQ=255)
               cTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCaaa                                                                                                                                      <  2:81302/150‑1 (MQ=255)
                 aTCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATc                                                                                                                                    >  1:1008812/1‑150 (MQ=255)
                 aTCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATc                                                                                                                                    >  1:1008811/1‑150 (MQ=255)
                 aTCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATc                                                                                                                                    >  1:1311812/1‑150 (MQ=255)
                  tCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCg                                                                                                                                   >  1:1169132/1‑150 (MQ=255)
                    gATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGAt                                                                                                                                 >  1:195735/1‑150 (MQ=255)
                       ttAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGcc                                                                                                                              >  2:1307970/1‑150 (MQ=255)
                             tttACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTAcc                                                                                                                        >  1:1471245/1‑150 (MQ=255)
                             tttACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTAcc                                                                                                                        >  1:1468139/1‑150 (MQ=255)
                             tttACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTAcc                                                                                                                        >  1:1467910/1‑150 (MQ=255)
                             tttACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTAcc                                                                                                                        >  1:1953150/1‑150 (MQ=255)
                              ttACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTTTTAAGTCTAGATAGCCAAATCGATGCCACTACCg                                                                                                                       <  2:1581216/150‑1 (MQ=255)
                                  ccTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTAc                                                                                                                   <  2:831657/150‑1 (MQ=255)
                                  ccTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTAc                                                                                                                   <  1:1358567/150‑1 (MQ=255)
                                  ccTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTAc                                                                                                                   <  2:2090065/150‑1 (MQ=255)
                                   cTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACg                                                                                                                  >  2:1154598/1‑150 (MQ=255)
                                    tGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGa                                                                                                                 >  1:1206075/1‑150 (MQ=255)
                                         gAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTaaa                                                                                                            >  2:2173539/1‑150 (MQ=255)
                                         gAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTaaa                                                                                                            <  2:465882/150‑1 (MQ=255)
                                           aaGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGt                                                                                                          <  2:1606506/150‑1 (MQ=255)
                                            aGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTg                                                                                                         >  2:624092/1‑150 (MQ=255)
                                               gATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGaaa                                                                                                      <  2:1993675/150‑1 (MQ=255)
                                                atatCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAg                                                                                                     <  2:1218886/150‑1 (MQ=255)
                                                      cTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCt                                                                                               >  1:1078956/1‑150 (MQ=255)
                                                       tACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAGGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCtt                                                                                              >  1:1478128/1‑150 (MQ=255)
                                                       tACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAGGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCtt                                                                                              >  1:1489848/1‑150 (MQ=255)
                                                       tACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAGGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCtt                                                                                              >  1:1916078/1‑150 (MQ=255)
                                                       tACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAGGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCtt                                                                                              >  1:1915982/1‑150 (MQ=255)
                                                        aCCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCttt                                                                                             <  1:2473387/150‑1 (MQ=255)
                                                              gTGCAGGCGCAGAAAGCCGGAATATCGCTGGTGGAAGAAGCCTGGGATCGCACCATCTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCTCGCTTTaataat                                                                                       >  1:1546377/1‑150 (MQ=255)
                                                                      gcAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGgatga                                                                               <  2:2267595/150‑1 (MQ=255)
                                                                      gcAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGgatga                                                                               <  2:2475061/150‑1 (MQ=255)
                                                                       cAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGgatgat                                                                              >  1:498555/1‑150 (MQ=255)
                                                                           aaGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATgcgc                                                                          <  1:2000713/150‑1 (MQ=255)
                                                                                 gAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTc                                                                    <  2:2010015/150‑1 (MQ=255)
                                                                                 gAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTc                                                                    <  2:1105422/150‑1 (MQ=255)
                                                                                 gAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTc                                                                    <  2:1105428/150‑1 (MQ=255)
                                                                                 gAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTc                                                                    <  1:2577127/150‑1 (MQ=255)
                                                                                 gAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTc                                                                    <  2:2310608/150‑1 (MQ=255)
                                                                                 gAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTc                                                                    >  2:130345/1‑150 (MQ=255)
                                                                                 gAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTc                                                                    >  2:130519/1‑150 (MQ=255)
                                                                                     aCCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCaa                                                                <  2:263813/150‑1 (MQ=255)
                                                                                       cGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATc                                                              >  1:796902/1‑150 (MQ=255)
                                                                                             tggtAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTg                                                        >  1:1297614/1‑150 (MQ=255)
                                                                                                tAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTa                                                     >  2:1312619/1‑150 (MQ=255)
                                                                                                  gAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGATAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAAc                                                   <  2:1468196/150‑1 (MQ=255)
                                                                                                  gAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAAc                                                   <  2:1468139/150‑1 (MQ=255)
                                                                                                  gAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAAc                                                   <  2:1953150/150‑1 (MQ=255)
                                                                                                        tGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACTCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCACTCAGTTTGTTAACgcgcgc                                             <  2:1434778/150‑1 (MQ=255)
                                                                                                        tGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTTAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACgcgcgc                                             <  2:305412/150‑1 (MQ=255)
                                                                                                        tGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACgcgcgc                                             <  2:305415/150‑1 (MQ=255)
                                                                                                        tGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACgcgcgc                                             <  2:1725158/150‑1 (MQ=255)
                                                                                                        tGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACgcgcgc                                             <  2:1431619/150‑1 (MQ=255)
                                                                                                          ggATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCAt                                           <  2:1266313/150‑1 (MQ=255)
                                                                                                          ggATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCAt                                           <  2:953160/150‑1 (MQ=255)
                                                                                                          ggATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACGACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCAt                                           <  2:2326567/150‑1 (MQ=255)
                                                                                                            aTCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGt                                         <  1:1634650/150‑1 (MQ=255)
                                                                                                              cGCACCAACTCGAAGACATTAAGTGCAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTa                                       <  1:2402911/150‑1 (MQ=255)
                                                                                                              cGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTa                                       <  1:691951/150‑1 (MQ=255)
                                                                                                                cACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGt                                     <  2:1661655/150‑1 (MQ=255)
                                                                                                                cACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGt                                     >  1:1531158/1‑150 (MQ=255)
                                                                                                                 aCCAACTCGAAGAAATTAAGTGACGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTc                                    <  1:234345/150‑1 (MQ=255)
                                                                                                                 aCCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTc                                    <  1:2008969/150‑1 (MQ=255)
                                                                                                                 aCCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTc                                    <  1:601713/150‑1 (MQ=255)
                                                                                                                          aagaaATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAACTCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGaa                           <  1:1809948/150‑1 (MQ=255)
                                                                                                                                ttAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAAc                     >  2:616816/1‑150 (MQ=255)
                                                                                                                                 tAAGTGAAGGCACGCTGTTAGGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACg                    <  2:1489848/150‑1 (MQ=255)
                                                                                                                                 tAAGTGAAGGCACGCTGTTAGGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACg                    <  2:1916078/150‑1 (MQ=255)
                                                                                                                                 tAAGTGAAGGCACGCTGTTAGGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACg                    <  2:1478128/150‑1 (MQ=255)
                                                                                                                                   aGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACGcc                  <  2:1352328/150‑1 (MQ=255)
                                                                                                                                   aGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACGcc                  <  2:39984/150‑1 (MQ=255)
                                                                                                                                      gAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACGCCGTa               >  1:1250080/1‑150 (MQ=255)
                                                                                                                                      gAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACGCCGTa               >  1:1250088/1‑150 (MQ=255)
                                                                                                                                        aGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACGCCGTAGt             >  1:1925679/1‑150 (MQ=255)
                                                                                                                                        aGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACGCCGTAGt             >  2:222364/1‑150 (MQ=255)
                                                                                                                                        aGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACGCCGTAGt             >  2:843489/1‑150 (MQ=255)
                                                                                                                                        aGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGATTCCCAATCAGTTTGTTAACGCGCGCATGTTATTCGACACCGAACAAAACGCCGAAGt             >  1:655555/1‑150 (MQ=255)
                                                                                                                                            aCGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACGCCGTAGTGATc         >  2:2565389/1‑150 (MQ=255)
                                                                                                                                               cTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACGCCGTAGTGATCCCa      >  2:719472/1‑150 (MQ=255)
                                                                                                                                               cTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACGCCGTAGTGATCCCa      >  2:719523/1‑150 (MQ=255)
                                                                                                                                                ttttAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACGCCGTAGTGATCCCaa     <  1:838539/148‑1 (MQ=255)
                                                                                                                                                 gTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACGCCGTAGTGATCCCAAc    <  2:1008811/150‑1 (MQ=255)
                                                                                                                                                 gTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACGCCGTAGTGATCCCAAc    <  2:1008812/150‑1 (MQ=255)
                                                                                                                                                  ttAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAACGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACGCCGTAGTGATCCCAACa   <  1:1307970/150‑1 (MQ=255)
                                                                                                                                                   tAAGTCTATATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACGCCGTAGTGATCCCAACAg  <  1:8758/150‑1 (MQ=255)
                                                                                                                                                   tAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACGCCGTAGTGATCCCAACAg  <  2:2095734/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAACGCCGTAGTGATCCCAACAG  >  NC_000913/2154698‑2154994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: