Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,219,632 G→A 8.4% intergenic (‑151/+60) osmF ← / ← bglX putative ABC transporter periplasmic binding protein/beta‑D‑glucoside glucohydrolase, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,219,6320GA 8.4% 458.8 / 24.1 142intergenic (‑151/+60)osmF/bglXputative ABC transporter periplasmic binding protein/beta‑D‑glucoside glucohydrolase, periplasmic
Reads supporting (aligned to +/- strand):  ref base G (72/58);  new base A (4/8);  total (76/66)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 153 total aligned reads displayed.

ATGCTTTCCTCATTCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCAT  >  NC_000913/2219483‑2219781
                                                                                                                                                     |                                                                                                                                                     
ctgCTTTCCTCATTCTTTTACTGTTGTTTTCAGCGAATTCAGAGAAAAGCATAGGTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATg                                                                                                                                                       <  2:427181/149‑1 (MQ=255)
atgCTTTCCTCATTCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATg                                                                                                                                                       <  2:828828/150‑1 (MQ=255)
atgCTTTCCTCATTCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATg                                                                                                                                                       <  2:155495/150‑1 (MQ=255)
    tttCCTCATTCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATgcggc                                                                                                                                                   >  2:150311/1‑150 (MQ=255)
        cTCATTCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGa                                                                                                                                               >  1:551223/1‑150 (MQ=255)
           aTTCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAgcg                                                                                                                                            >  2:2475042/1‑150 (MQ=255)
               ttttACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCtt                                                                                                                                        >  1:2194259/1‑150 (MQ=255)
               ttttACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATAAGGCGCTCGCGccgc                                                                                                                                        <  2:1574092/150‑3 (MQ=255)
               ttttACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATAAGGCGCTCGCGccgc                                                                                                                                        <  2:1574095/150‑3 (MQ=255)
               ttttACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATAAGGCGCTCGCGccgc                                                                                                                                        <  2:599369/150‑3 (MQ=255)
               ttttACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATAAGGCGCTCGCGccgc                                                                                                                                        <  2:599361/150‑3 (MQ=255)
                   aCTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATcc                                                                                                                                    <  1:2197773/150‑1 (MQ=255)
                    cTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCg                                                                                                                                   <  1:553523/150‑1 (MQ=255)
                    cTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCg                                                                                                                                   <  2:150650/150‑1 (MQ=255)
                      gttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGc                                                                                                                                 <  2:2047227/150‑1 (MQ=255)
                      gttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGc                                                                                                                                 <  2:2142208/150‑1 (MQ=255)
                      gttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGc                                                                                                                                 <  1:962118/150‑1 (MQ=255)
                       ttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGTATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATAAGGCGCTCGCGCCGCATCCGGca                                                                                                                                <  1:1813746/150‑2 (MQ=255)
                       ttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGTATAAGGCGCTCGCGCCGCATCCGGca                                                                                                                                <  1:2324555/150‑2 (MQ=255)
                       ttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGcc                                                                                                                                >  1:321587/1‑150 (MQ=255)
                       ttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGcc                                                                                                                                >  1:321596/1‑150 (MQ=255)
                       ttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGcc                                                                                                                                >  1:423481/1‑150 (MQ=255)
                       ttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGcc                                                                                                                                >  1:2360535/1‑150 (MQ=255)
                       ttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGcc                                                                                                                                >  1:511615/1‑150 (MQ=255)
                       ttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGcc                                                                                                                                >  1:81069/1‑150 (MQ=255)
                          ttttCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTAc                                                                                                                             >  1:2191107/1‑150 (MQ=255)
                          ttttCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTAc                                                                                                                             >  2:562716/1‑150 (MQ=255)
                           tttCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACa                                                                                                                            >  2:1242741/1‑150 (MQ=255)
                              cAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAg                                                                                                                         <  2:795159/150‑1 (MQ=255)
                              cAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAg                                                                                                                         <  2:1886622/150‑1 (MQ=255)
                                gCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAggg                                                                                                                       >  2:2221031/1‑150 (MQ=255)
                                   aaTTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGgcgc                                                                                                                    >  2:638506/1‑150 (MQ=255)
                                          agaAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGAAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTc                                                                                                             <  1:240055/150‑1 (MQ=255)
                                           gaAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAAGACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTcg                                                                                                            <  2:1867061/150‑1 (MQ=255)
                                            aaaaGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTcgc                                                                                                           >  1:600056/1‑150 (MQ=255)
                                              aaGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCgcgc                                                                                                         <  2:1752862/150‑1 (MQ=255)
                                              aaGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCgcgc                                                                                                         <  2:1952426/150‑1 (MQ=255)
                                              aaGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCgcgc                                                                                                         >  2:319818/1‑150 (MQ=255)
                                                  aTAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCtt                                                                                                     >  1:168199/1‑150 (MQ=255)
                                                      ttGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTa                                                                                                 >  1:806106/1‑150 (MQ=255)
                                                        gATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTaaa                                                                                               <  2:2093834/150‑1 (MQ=255)
                                                        gATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTaaa                                                                                               >  2:1390895/1‑150 (MQ=255)
                                                           aaTGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCa                                                                                            <  1:150311/150‑1 (MQ=255)
                                                           aaTGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCa                                                                                            <  1:244438/150‑1 (MQ=255)
                                                               gCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTAc                                                                                        >  2:916913/1‑150 (MQ=255)
                                                                cGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACa                                                                                       <  1:1026105/150‑1 (MQ=255)
                                                                cGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACa                                                                                       <  1:805546/150‑1 (MQ=255)
                                                                cGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACa                                                                                       >  1:1682215/1‑150 (MQ=255)
                                                                   ggTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCa                                                                                    <  2:2191111/150‑1 (MQ=255)
                                                                   ggTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCa                                                                                    <  2:2191107/150‑1 (MQ=255)
                                                                    gTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCaa                                                                                   <  2:1286839/150‑1 (MQ=255)
                                                                    gTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCCTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCaa                                                                                   <  2:1684549/150‑1 (MQ=255)
                                                                            gAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACt                                                                           <  1:210716/150‑1 (MQ=255)
                                                                              gCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCg                                                                         >  1:477010/1‑150 (MQ=255)
                                                                              gCGGAAGGATTCAGTTGCAGAATAAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCg                                                                         >  2:1962258/1‑150 (MQ=255)
                                                                               cGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACACAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGc                                                                        <  2:1496146/150‑1 (MQ=255)
                                                                               cGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGc                                                                        <  2:1496254/150‑1 (MQ=255)
                                                                               cGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGc                                                                        <  2:2155009/150‑1 (MQ=255)
                                                                               cGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGACGCATATTCGCTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGc                                                                        <  2:1099899/150‑1 (MQ=255)
                                                                                ggAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGcc                                                                       >  2:1896171/1‑150 (MQ=255)
                                                                                ggAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGACCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGcc                                                                       >  1:1164797/1‑150 (MQ=255)
                                                                                 gAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCt                                                                      <  1:1564733/150‑1 (MQ=255)
                                                                                  aaGTATTCAGTTGCATAATGAGATAAATACTTAAGTGGCATATTCGTTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAATCATTACAGCAACTCAATCTCGCCtt                                                                     <  1:271207/150‑1 (MQ=39)
                                                                                  aaGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCtt                                                                     >  1:1076074/1‑150 (MQ=255)
                                                                                  aaGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCGTACTAAAGCATTACAGCCACTCAAACTCGCCtt                                                                     >  1:260781/1‑150 (MQ=255)
                                                                                            ttGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAAcgcg                                                           >  2:2504889/1‑150 (MQ=255)
                                                                                            ttGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAAcgcg                                                           >  2:650868/1‑150 (MQ=255)
                                                                                               cAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGc                                                        >  1:1500665/1‑150 (MQ=255)
                                                                                               cAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGc                                                        >  1:158406/1‑150 (MQ=255)
                                                                                               cAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGc                                                        >  2:493628/1‑150 (MQ=255)
                                                                                                aGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCg                                                       >  2:1208864/1‑150 (MQ=255)
                                                                                                    tCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAAt                                                   >  2:1326288/1‑150 (MQ=255)
                                                                                                    tCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAAt                                                   >  1:1928110/1‑150 (MQ=255)
                                                                                                    tCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTGACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAAt                                                   >  1:1928130/1‑150 (MQ=255)
                                                                                                        aTAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGt                                               <  2:370816/150‑1 (MQ=255)
                                                                                                          aaaTACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGc                                             <  1:638506/150‑1 (MQ=255)
                                                                                                                   agagGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGa                                    >  1:1758410/1‑150 (MQ=255)
                                                                                                                          tatTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCTTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGaa                             >  2:2263326/1‑150 (MQ=255)
                                                                                                                          tatTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGACTCAGAGCCGATAAAGACATTGac                             >  2:758037/1‑149 (MQ=255)
                                                                                                                          tatTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGaa                             >  2:1812630/1‑150 (MQ=255)
                                                                                                                          tatTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGaa                             >  2:757791/1‑150 (MQ=255)
                                                                                                                           atTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAAc                            <  1:1318937/150‑1 (MQ=255)
                                                                                                                           atTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAAc                            <  2:257081/150‑1 (MQ=255)
                                                                                                                            ttCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACt                           >  2:1261209/1‑150 (MQ=255)
                                                                                                                                 tCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCa                      <  2:581578/150‑1 (MQ=255)
                                                                                                                                       gTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCg                <  2:879937/150‑1 (MQ=255)
                                                                                                                                          tCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCg             <  1:1808478/150‑1 (MQ=255)
                                                                                                                                           ccTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCATTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGt            >  1:858990/1‑150 (MQ=255)
                                                                                                                                            cTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTc           >  2:2353190/1‑150 (MQ=255)
                                                                                                                                             tGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCa          <  2:2370349/150‑1 (MQ=255)
                                                                                                                                             tGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCa          <  2:103372/150‑1 (MQ=255)
                                                                                                                                             tGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCa          <  2:103433/150‑1 (MQ=255)
                                                                                                                                             tGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCa          <  2:871869/150‑1 (MQ=255)
                                                                                                                                             ggCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCa          >  1:2121770/2‑150 (MQ=255)
                                                                                                                                              gCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACAAAGGGCGCAAACGACGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACAAAGAACTTGCCAGGCTCGGCGTcct         >  1:845611/1‑148 (MQ=255)
                                                                                                                                                cGGATAAGGCGCTCGCGCCGCATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCatat       >  2:1388718/1‑150 (MQ=255)
                                                                                                                                                   aTGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTc    >  1:771601/1‑150 (MQ=255)
                                                                                                                                                     gcggcgCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCAt  <  2:1682215/150‑1 (MQ=255)
                                                                                                                                                     gcggcgCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCAt  >  2:1525873/1‑150 (MQ=255)
                                                                                                                                                     gcggcgCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCAt  >  1:1740815/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGCTTTCCTCATTCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCAT  >  NC_000913/2219483‑2219781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: