Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,375,193 A→G 5.1% L257P (CTG→CCG)  menC ← O‑succinylbenzoyl‑CoA synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,375,1930AG 5.1% 683.1 / 16.9 197L257P (CTG→CCG) menCO‑succinylbenzoyl‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base A (106/81);  new base G (5/5);  total (111/86)
Fisher's exact test for biased strand distribution p-value = 7.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 197 total aligned reads displayed.

GCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAGGCAATGGCAA  >  NC_000913/2375045‑2375340
                                                                                                                                                    |                                                                                                                                                   
gCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAg                                                                                                                                                    >  2:2390868/1‑150 (MQ=255)
    cccGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGcccc                                                                                                                                                <  1:716453/150‑1 (MQ=255)
    cccGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGcccc                                                                                                                                                >  2:1273839/1‑150 (MQ=255)
       ggCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAgc                                                                                                                                             >  1:699523/1‑150 (MQ=255)
         ccAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAgcgc                                                                                                                                           <  2:170204/150‑1 (MQ=255)
         ccAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAgcgc                                                                                                                                           <  2:2439096/150‑1 (MQ=255)
          cAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGcgcg                                                                                                                                          <  2:801888/150‑1 (MQ=255)
           aGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGt                                                                                                                                         >  1:1181506/1‑150 (MQ=255)
           aGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGt                                                                                                                                         >  1:393356/1‑150 (MQ=255)
           aGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGt                                                                                                                                         >  1:1245922/1‑150 (MQ=255)
           aGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGt                                                                                                                                         >  2:779775/1‑150 (MQ=255)
           aGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGt                                                                                                                                         >  1:342789/1‑150 (MQ=255)
                cGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGcgcc                                                                                                                                    <  1:1028147/150‑1 (MQ=255)
                  tACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGcgccgc                                                                                                                                  <  2:247601/150‑1 (MQ=255)
                   aCCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCgccgcc                                                                                                                                 <  2:1411638/150‑1 (MQ=255)
                   aCCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCgccgcc                                                                                                                                 <  2:1411714/150‑1 (MQ=255)
                    ccTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCt                                                                                                                                >  1:2475114/1‑150 (MQ=255)
                    ccTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCt                                                                                                                                <  1:2161220/150‑1 (MQ=255)
                              cTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTc                                                                                                                      <  1:937499/150‑1 (MQ=255)
                                 cATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTcgcg                                                                                                                   >  1:2297582/1‑150 (MQ=255)
                                   tCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTa                                                                                                                 >  2:2357795/1‑150 (MQ=255)
                                   tCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTa                                                                                                                 >  1:1628903/1‑150 (MQ=255)
                                   tCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTa                                                                                                                 >  1:907319/1‑150 (MQ=255)
                                     aGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCATCCCCAGCGCGTGCGCCGCCTGTACCTGATCGCGTACt                                                                                                               >  1:181533/1‑150 (MQ=255)
                                       aTCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCGGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACttt                                                                                                             >  1:1195481/1‑150 (MQ=255)
                                       aTCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCGGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACttt                                                                                                             >  1:1584171/1‑150 (MQ=255)
                                       aTCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACttt                                                                                                             >  1:1487268/1‑150 (MQ=255)
                                          cAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTc                                                                                                          <  1:1941960/150‑1 (MQ=255)
                                          cAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTc                                                                                                          >  2:133432/1‑150 (MQ=255)
                                          cAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTc                                                                                                          <  1:1823956/150‑1 (MQ=255)
                                           aGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTcc                                                                                                         >  1:942966/1‑150 (MQ=255)
                                            gCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCa                                                                                                        <  2:212646/150‑1 (MQ=255)
                                            gCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCa                                                                                                        >  1:2191721/1‑150 (MQ=255)
                                              gtgtCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGa                                                                                                      <  1:386324/150‑1 (MQ=255)
                                                 tCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTg                                                                                                   >  1:475003/1‑150 (MQ=255)
                                                 tCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTg                                                                                                   >  1:1173846/1‑150 (MQ=255)
                                                 tCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTg                                                                                                   >  1:2505109/1‑150 (MQ=255)
                                                      cccTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGt                                                                                              >  1:1398653/1‑150 (MQ=255)
                                                        cTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGTTTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGa                                                                                            <  1:958166/150‑1 (MQ=255)
                                                        cTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGa                                                                                            <  1:71232/150‑1 (MQ=255)
                                                        cTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGa                                                                                            <  1:1046224/150‑1 (MQ=255)
                                                         tGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAg                                                                                           >  1:2245253/1‑150 (MQ=255)
                                                         tGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAg                                                                                           >  1:2076659/1‑150 (MQ=255)
                                                         tGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAg                                                                                           >  2:1501141/1‑150 (MQ=255)
                                                                  gtCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGttt                                                                                  <  2:2013861/150‑1 (MQ=255)
                                                                   tCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTg                                                                                 >  2:1077423/1‑150 (MQ=255)
                                                                   tCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTg                                                                                 >  2:1565569/1‑150 (MQ=255)
                                                                   tCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTg                                                                                 >  2:555173/1‑150 (MQ=255)
                                                                   tCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTg                                                                                 >  1:2233990/1‑150 (MQ=255)
                                                                     cGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGAt                                                                               >  1:1957576/1‑150 (MQ=255)
                                                                      ggCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTCAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTGTGATa                                                                              >  1:2215207/1‑150 (MQ=255)
                                                                       gCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATaa                                                                             >  1:2534907/1‑150 (MQ=255)
                                                                        cGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAAc                                                                            <  2:731341/150‑1 (MQ=255)
                                                                            aaCCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTAcc                                                                        <  1:2440823/150‑1 (MQ=255)
                                                                                aGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACcgcgc                                                                    >  1:650425/1‑150 (MQ=255)
                                                                                aGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACcgcgc                                                                    <  2:2238465/150‑1 (MQ=255)
                                                                                 ggcggcAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACcgcgcg                                                                   <  1:1388506/150‑1 (MQ=255)
                                                                                   cggcAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCa                                                                 <  2:700405/150‑1 (MQ=255)
                                                                                   cggcAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCa                                                                 <  2:954441/150‑1 (MQ=255)
                                                                                     gcAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACg                                                               >  1:78865/1‑150 (MQ=255)
                                                                                     gcAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACg                                                               >  1:1615169/1‑150 (MQ=255)
                                                                                       aaTCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGcc                                                             <  2:1948059/150‑1 (MQ=255)
                                                                                       aaTCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGcc                                                             <  2:2144418/150‑1 (MQ=255)
                                                                                         tCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCct                                                           <  2:2049500/150‑1 (MQ=255)
                                                                                           cgcgCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCctct                                                         >  1:180202/1‑150 (MQ=255)
                                                                                             cgcCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTc                                                       <  2:407941/150‑1 (MQ=255)
                                                                                                cAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGc                                                    >  1:159967/1‑150 (MQ=255)
                                                                                                cAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGc                                                    >  1:1731406/1‑150 (MQ=255)
                                                                                                 aGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGcc                                                   <  1:2035104/150‑1 (MQ=255)
                                                                                                      cGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACaaa                                              <  2:473302/150‑1 (MQ=255)
                                                                                                      cGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACaaa                                              <  2:2527944/150‑1 (MQ=255)
                                                                                                      cGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACaaa                                              <  2:163702/150‑1 (MQ=255)
                                                                                                      cGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACaaa                                              <  2:1184731/150‑1 (MQ=255)
                                                                                                       gTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAg                                             <  1:2485850/150‑1 (MQ=255)
                                                                                                             ccTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCaaaa                                       <  2:1216251/150‑1 (MQ=255)
                                                                                                             ccTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCaaaa                                       >  1:1882033/1‑150 (MQ=255)
                                                                                                                  gCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCgg                                  <  1:1197304/150‑1 (MQ=255)
                                                                                                                  gCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCgg                                  <  1:1155486/150‑1 (MQ=255)
                                                                                                                   cTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGc                                 >  2:548380/1‑150 (MQ=255)
                                                                                                                    tCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCt                                >  1:1329378/1‑150 (MQ=255)
                                                                                                                      gaatCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTcg                              >  1:2111554/4‑150 (MQ=255)
                                                                                                                      gATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTcg                              <  2:924414/150‑1 (MQ=255)
                                                                                                                          cAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCa                          <  2:2223845/150‑1 (MQ=255)
                                                                                                                            aTGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAgg                        <  2:297492/150‑1 (MQ=255)
                                                                                                                                  gaaCTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCa                  <  1:1967826/150‑1 (MQ=255)
                                                                                                                                  gaaCTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCa                  <  1:2338492/150‑1 (MQ=255)
                                                                                                                                   aaCTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCAt                 >  2:1763058/1‑150 (MQ=255)
                                                                                                                                   aaCTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCAt                 >  2:807029/1‑150 (MQ=255)
                                                                                                                                   aaCTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCAt                 >  1:551865/1‑150 (MQ=255)
                                                                                                                                   aaCTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCAt                 >  1:2582827/1‑150 (MQ=255)
                                                                                                                                      tGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATccc              >  1:1578951/1‑150 (MQ=255)
                                                                                                                                         tCACCGCCGTCGGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAgg           <  2:1584171/150‑1 (MQ=255)
                                                                                                                                         tCACCGCCGTCGGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAgg           <  2:653606/150‑1 (MQ=255)
                                                                                                                                         tCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAgg           >  1:769143/1‑150 (MQ=255)
                                                                                                                                         tCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAgg           >  1:1798645/1‑150 (MQ=255)
                                                                                                                                           aCCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCCGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAggca         >  1:1662256/1‑150 (MQ=255)
                                                                                                                                           aCCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAggca         >  2:1480615/1‑150 (MQ=255)
                                                                                                                                           aCCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCGTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAggcc         >  1:358794/1‑149 (MQ=255)
                                                                                                                                            ccgccgTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAggcaa        >  1:2372381/1‑150 (MQ=255)
                                                                                                                                             cgccgTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAggcaat       >  1:217196/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTTAACCAGGCGGCAATCCGCGCCAGTTGCGTTAAGCCTAAGCTCGATTCAATGGAAGAACTGATCACCGCCGTCAGCCCCAGCGCGTGCGCCGCCTGTACCTGCTCGCGTACTTTTTCCAGACTGCCCGTGAGCGTGGGTTTGATAACTACCGCGCGCACGCCCTCTTCAGCCACAAAGGCAAAATCCGGCTCGCGCAGGCTTTCATCCCAGGCAATGGCAA  >  NC_000913/2375045‑2375340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: