Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,679,430 Δ1 bp 6.3% intergenic (‑63/+34) yphF ← / ← yphG putative sugar ABC transporter periplasmic binding protein/DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,679,4300G. 6.3% 571.4 / 33.8 158intergenic (‑63/+34)yphF/yphGputative sugar ABC transporter periplasmic binding protein/DUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (67/80);  new base . (4/6);  total (72/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 161 total aligned reads displayed.

TTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCC  >  NC_000913/2679283‑2679579
                                                                                                                                                   |                                                                                                                                                      
     gCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGc                                                                                                                                                >  2:2786881/1‑150 (MQ=255)
      cAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGcc                                                                                                                                               <  2:2635579/150‑1 (MQ=255)
        gCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGc                                                                                                                                             >  2:2499458/1‑150 (MQ=255)
         cccTGGCAAATAGTGCGCTACCTAATCGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTGTGTAGGCATAGCGCCTCCTGTAGGGTTTTTATTAACACCGGCTTATTCTAATTATTTTGTGATGCGCGGCAGCGCGTGCCGCt                                                                                                                                            <  1:1253093/150‑1 (MQ=255)
         cccTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGAGCCGCt                                                                                                                                            >  1:371433/1‑150 (MQ=255)
          ccgggCAAATAGGGCGCTCCCTATTCGCGTAGCAATAAATAATTCATTCCGGTTTGTTCTCATTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTCa                                                                                                                                          <  1:2500068/147‑1 (MQ=255)
           cTGGCAAATAGTGCGCTACCTAATCGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCa                                                                                                                                          <  1:1854976/150‑1 (MQ=255)
           cTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCa                                                                                                                                          <  1:1854979/150‑1 (MQ=255)
            tGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCaa                                                                                                                                         >  1:260941/1‑150 (MQ=255)
            tGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCaa                                                                                                                                         >  1:2818478/1‑150 (MQ=255)
            tGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTCat                                                                                                                                         <  1:1694824/150‑2 (MQ=255)
            tGGAAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTCat                                                                                                                                         <  1:1039096/150‑2 (MQ=255)
            gggCAAATAGGGCGCGACCTAATCGCGTAGCCATTAATAATAGATTCCGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTCat                                                                                                                                         <  1:779421/149‑2 (MQ=255)
               cAAATAGTGCGCTACCTAATAGCGTATCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAata                                                                                                                                      <  1:2728909/150‑1 (MQ=255)
                 aaTAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATAtt                                                                                                                                    <  2:1430209/150‑1 (MQ=255)
                 aaTAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATAtt                                                                                                                                    >  1:1999153/1‑150 (MQ=255)
                    aGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACa                                                                                                                                 <  2:2317239/150‑1 (MQ=255)
                    aGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACa                                                                                                                                 <  1:213320/150‑1 (MQ=255)
                    aGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACa                                                                                                                                 <  2:2420442/150‑1 (MQ=255)
                    aGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACa                                                                                                                                 <  1:962702/150‑1 (MQ=255)
                    aGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACa                                                                                                                                 <  1:1300051/150‑1 (MQ=255)
                         gcTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTaa                                                                                                                            <  2:74056/150‑1 (MQ=255)
                         gcTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTaa                                                                                                                            <  2:1583518/150‑1 (MQ=255)
                         gcTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTaa                                                                                                                            <  1:1423341/150‑1 (MQ=255)
                           tACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATa                                                                                                                          >  1:2146306/1‑150 (MQ=255)
                              cTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAggg                                                                                                                       <  2:710804/150‑1 (MQ=255)
                                aaTAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGtt                                                                                                                     >  1:273191/1‑150 (MQ=255)
                                aaTAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGtt                                                                                                                     >  1:1772446/1‑150 (MQ=255)
                                aaTAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGACGCTCAAATATTACAGTTAATAGGGtt                                                                                                                     >  1:2863293/1‑150 (MQ=255)
                                  tAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTa                                                                                                                   <  1:2409164/150‑1 (MQ=255)
                                    gCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGATTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGt                                                                                                                 <  2:915137/150‑1 (MQ=255)
                                            aTTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAGTAGGGTTTAGTGAACGCgg                                                                                                         <  2:2206765/150‑1 (MQ=255)
                                              taataaTAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGtt                                                                                                       <  1:43220/150‑1 (MQ=255)
                                               aataatAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTa                                                                                                      >  1:449760/1‑150 (MQ=255)
                                                 taataGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTaaa                                                                                                    <  2:648366/150‑1 (MQ=255)
                                                  aataGATTTCGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTACTTTAGGTTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTaaaa                                                                                                   <  1:1823216/150‑1 (MQ=255)
                                                       aTTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATaaa                                                                                              <  1:1122686/150‑1 (MQ=255)
                                                            gggTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCa                                                                                         <  1:391945/150‑1 (MQ=255)
                                                              gttgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATc                                                                                       <  2:1999153/150‑1 (MQ=255)
                                                                  ttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACgg                                                                                   <  1:267970/150‑1 (MQ=255)
                                                                   tctcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggt                                                                                  <  2:2095084/150‑1 (MQ=255)
                                                                   tctcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTCTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggt                                                                                  <  2:2095089/150‑1 (MQ=255)
                                                                     tcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtgg                                                                                >  1:2901740/1‑150 (MQ=255)
                                                                     tcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtgg                                                                                >  2:621997/1‑150 (MQ=255)
                                                                     tcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtgg                                                                                >  1:1752230/1‑150 (MQ=255)
                                                                      cATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtggt                                                                               <  1:1268147/150‑1 (MQ=255)
                                                                      cATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtggt                                                                               <  1:2722143/150‑1 (MQ=255)
                                                                      cATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtggt                                                                               <  1:93564/150‑1 (MQ=255)
                                                                       aTTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTATTTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGgtggtg                                                                              >  2:149968/1‑150 (MQ=255)
                                                                       aTTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGgtggtg                                                                              >  2:2772354/1‑150 (MQ=255)
                                                                       aTTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGgtggtg                                                                              >  2:149981/1‑150 (MQ=255)
                                                                       aTTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGgtggtg                                                                              >  2:538073/1‑150 (MQ=255)
                                                                        tttttGTAGGCATAGAGCCTCCTGGAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGa                                                                             >  1:2581689/1‑150 (MQ=255)
                                                                         ttttGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGaa                                                                            <  2:337089/150‑1 (MQ=255)
                                                                         ttttGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGaa                                                                            >  2:868447/1‑150 (MQ=255)
                                                                         ttttGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGaa                                                                            >  1:101140/1‑150 (MQ=255)
                                                                            tGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGaataa                                                                         <  2:1379434/150‑1 (MQ=255)
                                                                             gTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGaataat                                                                        <  2:329455/150‑1 (MQ=255)
                                                                                   aTAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCt                                                                  >  1:2350422/1‑150 (MQ=255)
                                                                                       agCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCg                                                              <  2:2406958/150‑1 (MQ=255)
                                                                                        gCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCgg                                                             <  2:864145/150‑1 (MQ=255)
                                                                                         cctcctGTAGGGTTTTTATTAAAAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACATTTAAAATGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGc                                                            >  1:2273266/1‑150 (MQ=255)
                                                                                           tcctGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCa                                                          >  2:222875/1‑150 (MQ=255)
                                                                                            cctGTAGGGTTTTTATTAACAACGGCTTATTCTCATTATTTTGTGATGAGCGGCAGCGCGTGACGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTTAATAATGCCGCTTTCGGCCaa                                                         <  2:1637333/150‑1 (MQ=255)
                                                                                                   ggTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGAt                                                  <  2:1158758/150‑1 (MQ=255)
                                                                                                    gTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGAtt                                                 >  2:799959/1‑150 (MQ=255)
                                                                                                     tttttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTa                                                >  2:318641/1‑150 (MQ=255)
                                                                                                     tttttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTa                                                >  2:569182/1‑150 (MQ=255)
                                                                                                     tttttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTa                                                >  1:2572278/1‑150 (MQ=255)
                                                                                                      ttttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTaa                                               <  2:2087440/150‑1 (MQ=255)
                                                                                                           ttaACACCGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGcc                                          <  2:403272/150‑1 (MQ=255)
                                                                                                           ttaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGcc                                          >  2:2803266/1‑150 (MQ=255)
                                                                                                             aacaacGGCTTTTTCTATTTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGt                                        <  2:371433/150‑1 (MQ=255)
                                                                                                                aacGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCAGCTAAAAAATAACAGATAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCCAGCCGGATTAATCGCCGTCaa                                     >  2:1929825/1‑150 (MQ=255)
                                                                                                                    gCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCa                                 >  1:2629002/1‑150 (MQ=255)
                                                                                                                     cTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAg                                >  2:247906/1‑150 (MQ=255)
                                                                                                                     cTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAg                                >  2:2486187/1‑150 (MQ=255)
                                                                                                                       tATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAgcg                              <  2:2722010/150‑1 (MQ=255)
                                                                                                                           cTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAg                          >  2:110023/1‑150 (MQ=255)
                                                                                                                                    ttGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCat                 >  2:1154958/1‑150 (MQ=255)
                                                                                                                                    ttGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCat                 >  2:1339670/1‑150 (MQ=255)
                                                                                                                                     tgtgATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTTGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGACGTCAATTCAGCGCGAGCAGACTCata                <  1:2865779/150‑1 (MQ=255)
                                                                                                                                     tgtgATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCata                <  1:1108483/150‑1 (MQ=255)
                                                                                                                                          tgaGCGGCACGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTgcg           >  2:1076069/1‑150 (MQ=255)
                                                                                                                                           gaGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTgcgc          <  2:1803546/150‑1 (MQ=255)
                                                                                                                                           gaGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTgcgc          <  2:1775290/150‑1 (MQ=255)
                                                                                                                                           gaGCGGCACGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAATGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAAATCAGCGCGAGCAGACTCAAATTgcgc          >  1:1743960/1‑150 (MQ=255)
                                                                                                                                           gaGCGGCACGCGCTGCCGCTCAAATAGTACAGGTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGACTACTGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTgcgc          >  2:1684436/1‑150 (MQ=255)
                                                                                                                                            aGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa         <  1:2772354/150‑1 (MQ=255)
                                                                                                                                            aGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa         >  1:84197/1‑150 (MQ=255)
                                                                                                                                            aGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa         <  1:149981/150‑1 (MQ=255)
                                                                                                                                            aGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa         >  2:55421/1‑150 (MQ=255)
                                                                                                                                            aGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGAATCATATTGCGCa         >  1:465974/1‑150 (MQ=255)
                                                                                                                                             gCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCaa        >  1:2026542/1‑150 (MQ=255)
                                                                                                                                             gCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCaa        >  2:1864054/1‑150 (MQ=255)
                                                                                                                                              cGGCAGCGCGTGCCGCTCAAATATTACAGTTAATATGGTTTAGTGAAAGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc       >  1:1720153/1‑150 (MQ=255)
                                                                                                                                              cGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc       >  1:2071055/1‑150 (MQ=255)
                                                                                                                                              cGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc       >  1:1388076/1‑150 (MQ=255)
                                                                                                                                                 cAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTc    <  1:657606/150‑1 (MQ=255)
                                                                                                                                                   gcgcGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTccc  <  1:1837336/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
TTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCC  >  NC_000913/2679283‑2679579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: