Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,368,181 C→G 5.9% P119A (CCG→GCG) ‡ yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,1810CG 5.9% 545.6 / 18.3 149P119A (CCG→GCG) ‡yhcGDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
Reads supporting (aligned to +/- strand):  ref base C (82/58);  new base G (3/6);  total (85/64)
Fisher's exact test for biased strand distribution p-value = 1.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

GACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGC  >  NC_000913/3368032‑3368327
                                                                                                                                                     |                                                                                                                                                  
gACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAACTGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTACGCAGacac                                                                                                                                                    <  2:1645054/150‑1 (MQ=255)
gACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGacac                                                                                                                                                    <  2:1430942/150‑1 (MQ=255)
 aCTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACAcc                                                                                                                                                   <  1:1861345/150‑1 (MQ=255)
   tATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGt                                                                                                                                                 <  1:581155/150‑1 (MQ=255)
   tATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGt                                                                                                                                                 <  1:203078/150‑1 (MQ=255)
   tATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGt                                                                                                                                                 <  1:2031616/150‑1 (MQ=255)
      cAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTg                                                                                                                                              <  1:2703737/150‑1 (MQ=255)
        aaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGACTTAACACCATTGGGAATTCCGCAGACACCGTCTGCa                                                                                                                                            >  1:2635741/1‑150 (MQ=255)
          ggATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGa                                                                                                                                          >  1:2749931/1‑150 (MQ=255)
           gATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGaa                                                                                                                                         <  1:2649989/150‑1 (MQ=255)
                aTGTCTATGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCTGCAGACGGTGTCTGCGGAATTccc                                                                                                                                    <  2:2408178/150‑1 (MQ=255)
                aTGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAACCCGTCCGCCCAATGAGGCTTTTTTCCCTCTCTTTTCCACATGTGGAAATTCACCAGACAAGGTCTGGCGAATTAACACCATTGGGAATTCTGCAGACGGTGTCTGCGGAATTccc                                                                                                                                    <  2:2052989/150‑1 (MQ=255)
                aTGTATAAGGTATAAGCGTGGGTTCTCGGCAAACAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCTGCAGACGGTGTCTGCGGAATTccc                                                                                                                                    <  2:1622080/150‑1 (MQ=255)
                  gTCTTAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGTGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCTGCAGACGGTGTCTGCGGAATTCCCaa                                                                                                                                  <  2:934789/150‑2 (MQ=39)
                  gTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCTGCAGACGGTGTCTGCGGAATTCCCaa                                                                                                                                  <  2:1692537/150‑2 (MQ=39)
                   tCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTGTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATc                                                                                                                                 >  2:849923/1‑150 (MQ=255)
                    cTACGGTATAAGCGTGGGTTCTCTGCAAAAATCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACAGGTTGAAATTCACCAGACAATGTCTGGCGTATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCg                                                                                                                                <  2:2731393/150‑1 (MQ=255)
                    cTAAGGTATCTGCGTGGGTTCTCTGCAAAAAACTTCCGCCAAATGAGGCTTTTTTTCCTCTTTTTTCAACATGTTGAAATTCTCCAGACAATGTCTGGCGAATTTACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCg                                                                                                                                <  2:2731477/150‑1 (MQ=255)
                    cTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCg                                                                                                                                <  2:7068/150‑1 (MQ=255)
                    cTAAGGTAGAAGCGTGGGTTCTCTGCAAAAAACTTACGCCACCTGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCg                                                                                                                                <  2:1966764/150‑1 (MQ=255)
                     tAAGGTATAAGCGTGGTTTCTCTGAAAAAAACTTACGCCAACTGAGTCTTTTTTACCTCTTTTTTAAACATTTTGAAATTCACCAGAAAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCgg                                                                                                                               <  1:1763604/150‑1 (MQ=255)
                     tAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCgg                                                                                                                               >  2:331892/1‑150 (MQ=255)
                     tAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTATTGCGAATTAACACCATTGTGAATTCCGCAGACACCGTCTGCAGAATTCCCATCgg                                                                                                                               >  2:2883624/1‑150 (MQ=255)
                        ggTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCaa                                                                                                                            <  2:266123/150‑1 (MQ=255)
                         gTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATAAACACCATTGGGAATACCGCAGACACCCTCTGCAGAATTCCCATCGGCaaa                                                                                                                           >  1:1677578/1‑150 (MQ=255)
                               gCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTg                                                                                                                     >  2:1048096/1‑150 (MQ=255)
                               gCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTg                                                                                                                     >  2:1048087/1‑150 (MQ=255)
                                cGTGGGTTCTCTGCAAAAAACTTCCGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTgg                                                                                                                    <  1:2261398/150‑1 (MQ=255)
                                cGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCTAAGATTTgg                                                                                                                    <  2:1790815/150‑1 (MQ=255)
                                cGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGATTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTgg                                                                                                                    <  2:2265589/150‑1 (MQ=255)
                                    ggTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGa                                                                                                                >  1:1966881/1‑150 (MQ=255)
                                        ctctGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAcagc                                                                                                            <  1:210556/150‑4 (MQ=255)
                                        ctctGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCt                                                                                                            >  1:2504718/1‑150 (MQ=255)
                                         tctGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTg                                                                                                           >  1:1163719/1‑150 (MQ=255)
                                          ctGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCACTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTgc                                                                                                          <  1:2075095/150‑2 (MQ=255)
                                           tGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTc                                                                                                         >  1:415856/1‑150 (MQ=255)
                                           tGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTc                                                                                                         >  1:149925/1‑150 (MQ=255)
                                           tGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATGTGGCAGACGCTGTc                                                                                                         >  1:2728615/1‑150 (MQ=255)
                                            gCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTACCATCGGCAAAGATTTGGCAGACGCTGTCt                                                                                                        >  1:478636/1‑150 (MQ=255)
                                             cAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAAATCCCATCGTCAAAGATTTGGCAGACGCTGTCTg                                                                                                       >  2:2869879/1‑150 (MQ=255)
                                              aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGACTTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGc                                                                                                      >  1:2326284/1‑150 (MQ=255)
                                               aataaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGTCTAATTAACACCATTGGTAATTCCTCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGcc                                                                                                     <  2:2102128/147‑1 (MQ=255)
                                               aaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCCGACCCCGTCTGCAGACTTCCCATCGGCAAAGATTTGGCAGACGCGGTCTGcc                                                                                                     >  1:2013470/1‑150 (MQ=255)
                                                 aaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCaa                                                                                                   >  2:2025228/1‑150 (MQ=255)
                                                 aaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACAGCGTCTGCCaa                                                                                                   <  2:1196810/150‑1 (MQ=255)
                                                  aaCTAACGCCAAATGAGGCTTTTTTACCTATTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGTAATTCCGCAGACAGCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCaaa                                                                                                  <  1:1340568/150‑1 (MQ=255)
                                                   aCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAAt                                                                                                 >  2:1544923/1‑150 (MQ=255)
                                                   aCTTACGCCAAATGAGGCTTTTTTACCTATTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAAt                                                                                                 <  1:1060792/150‑1 (MQ=255)
                                                     ttACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCt                                                                                               >  2:317274/1‑150 (MQ=255)
                                                         gCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcc                                                                                           >  1:65928/1‑150 (MQ=255)
                                                         gCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCGGTCTGCCAAAACTTTcc                                                                                           >  1:1365212/1‑150 (MQ=255)
                                                          ccAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTccc                                                                                          >  1:2909871/1‑150 (MQ=255)
                                                          ccAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTccc                                                                                          <  2:2694009/150‑1 (MQ=255)
                                                            aaaTGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCt                                                                                        >  2:2339220/1‑150 (MQ=255)
                                                            aaaTGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAAATAACACCATTGGGAATTCCGCAGACACCGGCTGCAGCATTCCCATCGGCAAAGATTTGGCAGACCCGGTCTGCCAACTCTTTCCCCt                                                                                        >  1:1292272/1‑150 (MQ=255)
                                                                   gCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGTATTTCCGCAGACACCGTCTGAAGAATTCCCATCGGAAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAg                                                                                 <  1:972274/150‑1 (MQ=255)
                                                                     ttttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAAGTCCGCAGACACCGTCTGCCGAATTCCCATCGGCAAAGATGTGGAAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGt                                                                               >  2:1909070/1‑150 (MQ=255)
                                                                        tttACCTCTTTTTTCAACATGTTGAAATTCACCAGACATTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCa                                                                            <  1:2547962/150‑1 (MQ=255)
                                                                        tttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCa                                                                            <  2:1645918/150‑1 (MQ=255)
                                                                             ctctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTAc                                                                       >  2:1168618/1‑150 (MQ=255)
                                                                             ctctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTAc                                                                       >  2:2834112/1‑150 (MQ=255)
                                                                             ctctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTAc                                                                       >  1:974951/1‑150 (MQ=255)
                                                                             ctctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCGTGCCCAGGTCCACCTAc                                                                       >  1:1444996/1‑150 (MQ=255)
                                                                              tctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACg                                                                      >  1:416654/1‑150 (MQ=255)
                                                                                ttttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCTGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCACATCTTTGCCAGTGCCCAGGGCCGCCTGCggc                                                                    >  1:1834556/1‑148 (MQ=255)
                                                                                ttttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTc                                                                    >  2:1088471/1‑150 (MQ=255)
                                                                                   tttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGACTTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGt                                                                 >  2:1147287/1‑150 (MQ=255)
                                                                                   tttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGt                                                                 >  1:2165900/1‑150 (MQ=255)
                                                                                   tttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGt                                                                 >  1:2323512/1‑150 (MQ=255)
                                                                                    ttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTGTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGtgggtt                                                                >  2:753131/1‑145 (MQ=255)
                                                                                        aCATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACt                                                            >  2:387335/1‑150 (MQ=255)
                                                                                            gTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTcc                                                        <  1:164949/150‑1 (MQ=255)
                                                                                            gTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTcc                                                        <  1:164952/150‑1 (MQ=255)
                                                                                                   ttCGCCAGACATTGTCTGGTGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaaa                                                 >  1:728078/1‑150 (MQ=255)
                                                                                                   ttCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaaa                                                 >  2:1499099/1‑150 (MQ=255)
                                                                                                       ccAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCGGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGc                                             <  1:802310/150‑1 (MQ=255)
                                                                                                         aGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTg                                           <  2:2909871/150‑1 (MQ=255)
                                                                                                             aaTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGc                                       >  2:1828394/1‑150 (MQ=255)
                                                                                                             aaTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGc                                       <  1:1279439/150‑1 (MQ=255)
                                                                                                               tGTCGGGCGAATTAACACCCTTGGGATTTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGccc                                     <  1:2413812/150‑1 (MQ=255)
                                                                                                                   tGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGGATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAg                                 >  2:1951306/1‑150 (MQ=255)
                                                                                                                    ggCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGGTTACGTTCCGTTAAAAAAGCTGACGCCCGCAgg                                >  1:2125678/1‑149 (MQ=255)
                                                                                                                     gCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCt                               <  1:1033837/150‑1 (MQ=255)
                                                                                                                         cttAACCCCCTGGGGAAGTCCGCAGACACCGTCTGCAGAATTCCCATCGGCACCGATTGGGCAGCCGCTGTCTGCCAAATCTTTCCCCGGGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTa                           <  1:2855211/149‑1 (MQ=255)
                                                                                                                         aTTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTa                           <  1:1350278/150‑1 (MQ=255)
                                                                                                                          ttAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTAt                          >  2:1925966/1‑150 (MQ=255)
                                                                                                                          ttAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTAt                          >  2:722986/1‑150 (MQ=255)
                                                                                                                          ttAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCAGTCTGCCAAATCTGTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTGTAt                          >  2:2815475/1‑150 (MQ=255)
                                                                                                                            aaCACCCGTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGa                        <  2:2013470/150‑1 (MQ=255)
                                                                                                                               acCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAATGATTTGGCAGACGGTGTCTGGCAAATCTTTCGCCTTGCCCAGGTCCACCTACGTGCGTTTTCTTTCCGTTAAATTCGCTGAGGCCCGCAGCTTTTTTgtagt                     >  2:1270961/1‑146 (MQ=255)
                                                                                                                                  aTTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAaga                  <  1:1923516/150‑1 (MQ=255)
                                                                                                                                   ttGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTTGCAGACGCTGTCTGCCAAATCATTCCCCTTGCCCAGGTCCAACAACCTCCCTTTAAATTCCGTTAAAAACGCATACGCCCGCAGCTTTTATGAAAAagag                 >  1:2193968/1‑150 (MQ=255)
                                                                                                                                   atGGGAATTCTGCAGACGGTGTCTGCGGAATTCCCATCGGCAATGATTTGGCAGACGCTGTCTGCCAAATCTTTACCCGTGCCCAGGTCCACCAACGTCCGTTTACTTTACGTTAAAAACGCTGACGCCCCCAGCGTTTATGAAAAagag                 >  1:705098/2‑150 (MQ=37)
                                                                                                                                          ttCTGCAGACGGTGTCTGCGGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTcc          >  2:2152101/1‑150 (MQ=255)
                                                                                                                                          ttCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATATTTCCCCTTGCCCCGGTCCACCTACGTCCGGTTACTTTCCGTTAAAAACGCGGACGCCCGCCGCTTTTATGAAAAAGAGACGCTcc          >  1:2550808/1‑150 (MQ=255)
                                                                                                                                             cGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCt       <  1:2339220/150‑1 (MQ=255)
                                                                                                                                              gCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtg      <  2:378176/150‑1 (MQ=255)
                                                                                                                                               cAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtgt     <  2:175177/150‑1 (MQ=255)
                                                                                                                                               cAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtgt     <  1:1168618/150‑1 (MQ=255)
                                                                                                                                                  acacCGTCTGCAGAATTCCCATCGGCAAAGATTTTGCAGACGCTGTCTGCCAAAACATTCCCCTTGCCCAGGTCCACCGACGTCCCGTTACTTTACGTTAAAAACGCTGAAGCCCGCAGCGTTTATGAAAAAGAGACGCTCCGCGGTGGc  >  1:439256/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGC  >  NC_000913/3368032‑3368327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: