Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,377,769 G→T 5.3% intergenic (‑349/+46) sspA ← / ← rpsI stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog/30S ribosomal subunit protein S9

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,377,7690GT 5.3% 686.8 / 16.2 187intergenic (‑349/+46)sspA/rpsIstringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog/30S ribosomal subunit protein S9
Reads supporting (aligned to +/- strand):  ref base G (89/87);  new base T (7/4);  total (96/91)
Fisher's exact test for biased strand distribution p-value = 5.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

GGGAAAAAGAGATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAG  >  NC_000913/3377620‑3377916
                                                                                                                                                     |                                                                                                                                                   
gggAAAAAGAGATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACccgccg                                                                                                                                                     >  1:1936767/1‑150 (MQ=255)
 ggAAAAAGAGATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGATATAAAAAAACCCGCCGa                                                                                                                                                    >  1:1176217/1‑150 (MQ=255)
  gAAAAAGAGATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGaa                                                                                                                                                   <  2:629432/150‑1 (MQ=255)
     aaaGAGATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAcgcg                                                                                                                                                >  2:2520259/1‑150 (MQ=255)
     aaaGAGATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCg                                                                                                                                                <  2:1071287/150‑1 (MQ=255)
     aaaGAGATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCg                                                                                                                                                >  2:1514433/1‑150 (MQ=255)
       agagATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCggg                                                                                                                                              <  2:1222349/150‑1 (MQ=255)
          gaTGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGttt                                                                                                                                           <  2:1600040/150‑1 (MQ=255)
          gaTGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCATATTGGCGATGTGGTTTTTGTTTTACCGGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCAGCGTCAGAGTTTAGCTATAAAAAAACCCGCCGAAGCGGGttt                                                                                                                                           >  1:1125945/1‑150 (MQ=255)
            tGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGttttt                                                                                                                                         <  1:2698207/150‑1 (MQ=255)
             gcgcTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGtttttt                                                                                                                                        <  2:2470123/150‑1 (MQ=255)
             gcgcTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGtttttt                                                                                                                                        <  2:2522627/150‑1 (MQ=255)
             gcgcTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGtttttt                                                                                                                                        >  1:2408494/1‑150 (MQ=255)
                cTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGa                                                                                                                                     <  2:2203560/150‑1 (MQ=255)
                 tttAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCTTCGGCGGGtttttttata                                                                                                                                    <  1:1698699/150‑5 (MQ=255)
                   tAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCGGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGaaac                                                                                                                                  <  2:473837/150‑2 (MQ=255)
                        tGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGtt                                                                                                                             >  2:1837580/1‑150 (MQ=255)
                         gAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATGTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGttt                                                                                                                            >  2:4711/1‑150 (MQ=255)
                          aaaTAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGtttt                                                                                                                           <  1:370216/150‑1 (MQ=255)
                              aGTTGACTTAGTCCCTTATTGGCGTTGTGTTTTTTTTTTTACCTGTCTGTCAGGTGGCTGAAAAAAGCAACTTTCCAGTTTTTACGCTTATACAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGc                                                                                                                       <  1:1018484/150‑1 (MQ=255)
                              aGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGc                                                                                                                       <  1:2458829/150‑1 (MQ=255)
                               gTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGcc                                                                                                                      >  2:2890791/1‑150 (MQ=255)
                                ttGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCg                                                                                                                     <  2:1007113/150‑1 (MQ=255)
                                  gACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTGTGCCGGa                                                                                                                   >  1:2235009/1‑150 (MQ=255)
                                  gACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGa                                                                                                                   <  2:229231/150‑1 (MQ=255)
                                   aCTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAg                                                                                                                  >  2:194461/1‑150 (MQ=255)
                                         tCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAg                                                                                                            >  2:2432310/1‑150 (MQ=255)
                                           ccTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTAAGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTATGCCGGAGCAGAAGcc                                                                                                          >  1:2527659/1‑150 (MQ=255)
                                           ccTTATTGGCGATGTGGTTTTTGTTTTACCAGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAAACCGCCGAAGCGGGTTTTTTCGAAAATTTTTTTATTCCGGAGCAGAAGcc                                                                                                          >  1:1278645/1‑150 (MQ=255)
                                           ccTTATTGGCGATGTGGTTTTTGTTTTAACGGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCGGATTCAGATTTTAGCTAAAAAAAAAACCGCCGAAGCGGGTTTTTTCGAAAATTTTTTTCTGCCGGAGCAGAAGcc                                                                                                          >  1:2632653/1‑150 (MQ=255)
                                            cTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCa                                                                                                         >  2:231730/1‑150 (MQ=255)
                                             ttATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCaa                                                                                                        <  1:664293/150‑1 (MQ=255)
                                                   gCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACg                                                                                                  >  2:669217/1‑150 (MQ=255)
                                                   gCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACg                                                                                                  >  2:1248162/1‑150 (MQ=255)
                                                    cGATGTGGTTTTTTTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTAAGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTATGCCGGAGCAGAAGCCAATTAACGt                                                                                                 >  1:1810058/1‑150 (MQ=255)
                                                        gtgGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTgg                                                                                             <  2:1445936/150‑1 (MQ=255)
                                                         tgGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGga                                                                                            <  1:581252/150‑1 (MQ=255)
                                                         tgGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGga                                                                                            <  1:1778631/150‑1 (MQ=255)
                                                          ggTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGgag                                                                                           >  1:731656/1‑150 (MQ=255)
                                                            tttttGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGaa                                                                                         >  2:1396015/1‑150 (MQ=255)
                                                                tgtatgaCCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGATTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGc                                                                                     <  1:697882/144‑1 (MQ=255)
                                                                tGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGc                                                                                     <  1:457625/150‑1 (MQ=255)
                                                                 gTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCg                                                                                    <  1:2335898/150‑1 (MQ=255)
                                                                 gTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCg                                                                                    <  1:868863/150‑1 (MQ=255)
                                                                  ttttACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCgg                                                                                   >  2:401961/1‑150 (MQ=255)
                                                                  ttttACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCgg                                                                                   >  2:330310/1‑150 (MQ=255)
                                                                      aCCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGTTTCAGATTTTAGCTATAAAAATACCCGCCGAAGCGGGTTTTTTCGTAATTTGTTTTCTGCCGGCGCAGTAGGGAATTAACGTGTGGAGAACTGCGgacga                                                                               >  2:2536899/1‑150 (MQ=255)
                                                                      aCCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacga                                                                               <  1:1855279/150‑1 (MQ=255)
                                                                        ctgtctgtCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacg                                                                             >  1:2896932/1‑150 (MQ=255)
                                                                        ctgtctgtCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacg                                                                             >  2:2808460/1‑150 (MQ=255)
                                                                        ctgtctgtCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacg                                                                             >  1:703370/1‑150 (MQ=255)
                                                                        ctgtctgtCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacg                                                                             >  1:2882234/1‑150 (MQ=255)
                                                                        ctgtctgtCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGGTTGGAGAACTGCGgacgacg                                                                             >  1:2894672/1‑150 (MQ=255)
                                                                        ctgtctgtCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTATACGCTGATACAGATTTTAGCTATAAAAAAAAAAGCCGAAGCGGGATATTTCGAAAATAGTTAACTGCAGTAGCAGAAGCCAATAAACGTTTGGAGAAATGCGgaagacc                                                                             >  2:2166707/1‑149 (MQ=255)
                                                                        ctgtctgtCAGGTGGCAGAAAAAAGCAACTTTCCAGTTTTTACGCTGATTCATATTTTAGCTATAAAAAAAGCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacg                                                                             <  1:2220662/150‑1 (MQ=255)
                                                                         tgtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacga                                                                            >  2:2248634/1‑150 (MQ=255)
                                                                         tgtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacga                                                                            >  2:2771380/1‑150 (MQ=255)
                                                                          gtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacgac                                                                           <  1:1099885/150‑1 (MQ=255)
                                                                          gtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATGTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacgac                                                                           >  2:1484119/1‑150 (MQ=255)
                                                                             ggacAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGAGTCAGATTTTAGCGATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCGGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTg                                                                        <  2:1087376/147‑1 (MQ=255)
                                                                                  ggTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGACAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTa                                                                   >  2:15303/1‑150 (MQ=255)
                                                                                   gTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTAc                                                                  <  1:441875/150‑1 (MQ=255)
                                                                                    tGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACg                                                                 <  1:1813522/150‑1 (MQ=255)
                                                                                    tGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTGTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACg                                                                 >  2:501678/1‑150 (MQ=255)
                                                                                      gcagcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCa                                                               >  1:1564314/1‑150 (MQ=255)
                                                                                        agcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGa                                                             >  2:2714299/1‑150 (MQ=255)
                                                                                         gcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGAc                                                            <  2:1875914/150‑1 (MQ=255)
                                                                                           aaaaaGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCg                                                          >  2:855824/1‑150 (MQ=255)
                                                                                           aaaaaGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCg                                                          >  1:1022796/1‑150 (MQ=255)
                                                                                           aaaaaGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCg                                                          >  1:793127/1‑150 (MQ=255)
                                                                                            aaaaGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGa                                                         >  1:187597/1‑150 (MQ=255)
                                                                                            aaaaGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGa                                                         <  1:815123/150‑1 (MQ=255)
                                                                                                 cAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTc                                                    <  2:2408494/150‑1 (MQ=255)
                                                                                                  aaCTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCt                                                   >  2:2103928/1‑150 (MQ=255)
                                                                                                  aaCTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCt                                                   >  1:2565840/1‑150 (MQ=255)
                                                                                                  aaCTTTCCAGTTTTTACGCTGATTCAGATGTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCt                                                   >  2:1751314/1‑150 (MQ=255)
                                                                                                       tCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGt                                              >  2:514687/1‑150 (MQ=255)
                                                                                                         cAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTc                                            >  2:1732109/1‑150 (MQ=255)
                                                                                                           gTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCaa                                          >  1:2794875/1‑150 (MQ=255)
                                                                                                            tttttACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAAc                                         <  1:2749134/150‑1 (MQ=255)
                                                                                                            tttttACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAAc                                         <  1:89378/150‑1 (MQ=255)
                                                                                                                     tGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGc                                >  2:1201429/1‑150 (MQ=255)
                                                                                                                        ttCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTc                             <  2:754219/150‑1 (MQ=255)
                                                                                                                         tCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCa                            >  1:1181944/1‑150 (MQ=255)
                                                                                                                          cAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCAc                           <  2:253222/150‑1 (MQ=255)
                                                                                                                          cAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCAc                           <  1:2606326/150‑1 (MQ=255)
                                                                                                                          cAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCAc                           >  1:1431947/1‑150 (MQ=255)
                                                                                                                                ttAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCTGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTaa                     <  2:1656377/150‑1 (MQ=255)
                                                                                                                                  aGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACg                   <  1:2653572/150‑1 (MQ=255)
                                                                                                                                  aGCTAAAAAAAAACCCGCCGAAGCGGGATTTTTCGAAAATTGATTTCTGCCGGAGCAGAAGCCAATTAACGATTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACg                   <  2:1784290/150‑1 (MQ=255)
                                                                                                                                      ataAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGc               >  1:2683032/1‑150 (MQ=255)
                                                                                                                                         aaaaaaCCCGCTTCGGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAg            >  1:119284/1‑150 (MQ=255)
                                                                                                                                          aaaaaCCCGCTTCGGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGc           >  1:764295/1‑150 (MQ=255)
                                                                                                                                          aaaaaCCCGCTTCGGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGc           >  1:1759066/1‑150 (MQ=255)
                                                                                                                                          aaaaaCCCGCTTCGGCGGGTTTTTTCGAAAATTGTTTTATGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGc           >  1:679683/1‑150 (MQ=255)
                                                                                                                                              aCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTa       <  1:669217/150‑1 (MQ=255)
                                                                                                                                              aCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTa       <  1:1248162/150‑1 (MQ=255)
                                                                                                                                                  gccgAAGCGGGTTTTTTCGAAAATTGTTTTCTGTCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTAACGTTCAACCTGACGAGCGTCACGAGAAACGAAGCCAGCATTACGCa   >  1:2205366/1‑150 (MQ=255)
                                                                                                                                                   ccgAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAg  <  1:2126903/150‑1 (MQ=255)
                                                                                                                                                   ccgAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAg  >  2:2836446/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GGGAAAAAGAGATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAG  >  NC_000913/3377620‑3377916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: