Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,377,771 A→G 5.8% intergenic (‑351/+44) sspA ← / ← rpsI stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog/30S ribosomal subunit protein S9

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,377,7710AG 5.8% 635.0 / 19.2 188intergenic (‑351/+44)sspA/rpsIstringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog/30S ribosomal subunit protein S9
Reads supporting (aligned to +/- strand):  ref base A (89/88);  new base G (7/4);  total (96/92)
Fisher's exact test for biased strand distribution p-value = 5.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

Only 100 of 193 total aligned reads displayed.

GAAAAAGAGATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTC  >  NC_000913/3377622‑3377919
                                                                                                                                                     |                                                                                                                                                    
gAAAAAGAGATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGaa                                                                                                                                                      <  2:629432/150‑1 (MQ=255)
   aaaGAGATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCg                                                                                                                                                   >  2:2520282/1‑150 (MQ=255)
    aaGAGATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCgg                                                                                                                                                  <  2:1221129/150‑1 (MQ=255)
       agaTGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGtt                                                                                                                                               <  2:1620655/150‑1 (MQ=255)
        gaTGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCATATTGGCGATGTGGTTTTTGTTTTACCGGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCAGCGTCAGAGTTTAGCTATAAAAAAACCCGCCGAAGCGGGttt                                                                                                                                              >  1:1125945/1‑150 (MQ=255)
         aTGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGtttt                                                                                                                                             <  2:1766891/150‑1 (MQ=255)
         aTGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGtttt                                                                                                                                             <  1:178167/150‑1 (MQ=255)
         aTGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGtttt                                                                                                                                             <  1:177815/150‑1 (MQ=255)
          tGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGttttt                                                                                                                                            <  1:2698207/150‑1 (MQ=255)
           gcgcTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGtttttt                                                                                                                                           <  2:2470122/150‑1 (MQ=255)
           gcgcTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGtttttt                                                                                                                                           >  1:2408494/1‑150 (MQ=255)
           gcgcTTTAGTCTGAAATAGTTGACTTAGTCCCTTAGTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGtttttt                                                                                                                                           <  2:1511030/150‑1 (MQ=255)
               tttAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCTTCGGCGGGtttttttata                                                                                                                                       <  1:1698687/150‑5 (MQ=255)
               tttAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCTTCGGCGGGtttttttata                                                                                                                                       <  1:1698811/150‑5 (MQ=255)
               tttAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGaa                                                                                                                                       >  2:2429923/1‑150 (MQ=255)
                 tAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCGGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGaaac                                                                                                                                     <  2:473837/150‑2 (MQ=255)
                      tGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGtt                                                                                                                                <  2:1460102/150‑1 (MQ=255)
                      tGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGtt                                                                                                                                >  2:2218734/1‑150 (MQ=255)
                       gAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATGTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGttt                                                                                                                               >  2:4711/1‑150 (MQ=255)
                        aaaTAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGtttt                                                                                                                              <  1:370216/150‑1 (MQ=255)
                            aGTTGACTTAGTCCCTTATTGGCGTTGTGTTTTTTTTTTTACCTGTCTGTCAGGTGGCTGAAAAAAGCAACTTTCCAGTTTTTACGCTTATACAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGc                                                                                                                          <  1:1018484/150‑1 (MQ=255)
                            aGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGc                                                                                                                          <  1:2458829/150‑1 (MQ=255)
                             gTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGcc                                                                                                                         >  2:1078776/1‑150 (MQ=255)
                             gTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGcc                                                                                                                         >  2:2890791/1‑150 (MQ=255)
                             gTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGcc                                                                                                                         >  1:670466/1‑150 (MQ=255)
                             gTTGACATAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGcc                                                                                                                         <  1:818382/150‑1 (MQ=255)
                                gACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTGTGCCGGa                                                                                                                      >  1:2235009/1‑150 (MQ=255)
                                gACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGa                                                                                                                      <  2:1128881/150‑1 (MQ=255)
                                gACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGa                                                                                                                      >  2:2800755/1‑150 (MQ=255)
                                gACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGa                                                                                                                      <  2:132681/150‑1 (MQ=255)
                                   ttAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACATTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCa                                                                                                                   >  1:732063/1‑150 (MQ=255)
                                         ccTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGcc                                                                                                             >  2:2503075/1‑150 (MQ=255)
                                         ccTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTAAGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTATGCCGGAGCAGAAGcc                                                                                                             >  1:2527659/1‑150 (MQ=255)
                                          cTTATTGGCGATGTGGTTTTTGTTTTAACAGTCTGTCAGGTGGCAGCAAAAAGCAACATTCCAGATTTTACGCTGATTCAGATTTTAGATATAAAAAAACCCGCCGAAGCGGGTTTTTTAGAAAATTGTTTTATGCCGGAGCAGAAGCCa                                                                                                            >  1:1814924/1‑150 (MQ=255)
                                           ttATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCaa                                                                                                           <  2:1804955/150‑1 (MQ=255)
                                             aTTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAAtt                                                                                                         <  2:1858317/150‑1 (MQ=255)
                                                 gCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACg                                                                                                     >  2:1248162/1‑150 (MQ=255)
                                                 gCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACg                                                                                                     >  2:669217/1‑150 (MQ=255)
                                                  cGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGt                                                                                                    >  2:1895943/1‑150 (MQ=255)
                                                    aTGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGttt                                                                                                  <  2:1402465/150‑1 (MQ=255)
                                                       tgGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGga                                                                                               <  1:581197/150‑1 (MQ=255)
                                                          tttttGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGaa                                                                                            <  1:1177000/150‑1 (MQ=255)
                                                            tttGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACt                                                                                          >  1:623359/1‑150 (MQ=255)
                                                               gTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCg                                                                                       <  1:2335898/150‑1 (MQ=255)
                                                                ttttACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCgg                                                                                      >  2:401961/1‑150 (MQ=255)
                                                                ttttACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCgg                                                                                      >  2:330310/1‑150 (MQ=255)
                                                                  ttACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgac                                                                                    >  2:218880/1‑150 (MQ=255)
                                                                    aCCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGTTTCAGATTTTAGCTATAAAAATACCCGCCGAAGCGGGTTTTTTCGTAATTTGTTTTCTGCCGGCGCAGTAGGGAATTAACGTGTGGAGAACTGCGgacga                                                                                  >  2:2536899/1‑150 (MQ=255)
                                                                    aCCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTATCGAAAATTGTTTTCTGCCGGAGCTGAAGGCAATTAACGATTGGAGAACTGCGgacga                                                                                  >  2:1739358/1‑150 (MQ=255)
                                                                      ctgtctgtCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacg                                                                                >  1:703616/1‑150 (MQ=255)
                                                                      ctgtctgtCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacg                                                                                >  1:1272107/1‑150 (MQ=255)
                                                                      ctgtctgtCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacg                                                                                >  2:2808460/1‑150 (MQ=255)
                                                                      ctgtctgtCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacg                                                                                >  1:2882234/1‑150 (MQ=255)
                                                                      ctgtctgtCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacg                                                                                >  1:2896932/1‑150 (MQ=255)
                                                                      ctgtctgtCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTGTGGAGAACTGCGgacgacg                                                                                >  2:1068475/1‑150 (MQ=255)
                                                                      ctgtctgtCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGGTTGGAGAACTGCGgacgacg                                                                                >  1:2894672/1‑150 (MQ=255)
                                                                      ctgtctgtCAGGTGGCAGAAAAAAGCAACTTTCCAGTTTTTACGCTGATTCATATTTTAGCTATAAAAAAAGCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacg                                                                                <  1:2220662/150‑1 (MQ=255)
                                                                       tgtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacga                                                                               >  2:2248754/1‑150 (MQ=255)
                                                                        gtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacgac                                                                              <  1:700974/150‑1 (MQ=255)
                                                                        gtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacgac                                                                              <  1:1099885/150‑1 (MQ=255)
                                                                           tgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTg                                                                           <  2:1598959/150‑1 (MQ=255)
                                                                                ggTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTa                                                                      >  2:2299614/1‑150 (MQ=255)
                                                                                 gTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTAc                                                                     <  1:441875/150‑1 (MQ=255)
                                                                                  tGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACg                                                                    <  1:1813522/150‑1 (MQ=255)
                                                                                  tGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACg                                                                    <  1:1222350/150‑1 (MQ=255)
                                                                                  tGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTGTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACg                                                                    >  2:501678/1‑150 (MQ=255)
                                                                                      agcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGa                                                                >  2:1710647/1‑150 (MQ=255)
                                                                                      agcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGa                                                                >  2:2714299/1‑150 (MQ=255)
                                                                                         aaaaaGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCg                                                             >  2:855824/1‑150 (MQ=255)
                                                                                         aaaaaGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCg                                                             >  1:1022796/1‑150 (MQ=255)
                                                                                          aaaaGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGa                                                            >  1:187529/1‑150 (MQ=255)
                                                                                          aaaaGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGa                                                            <  1:1895943/150‑1 (MQ=255)
                                                                                           aaaGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGAc                                                           >  1:69119/1‑150 (MQ=255)
                                                                                                aaCTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCt                                                      >  2:2103928/1‑150 (MQ=255)
                                                                                                aaCTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCt                                                      >  1:2565840/1‑150 (MQ=255)
                                                                                                aaCTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCATCCGGAGCAGAAGCCAATTAACGTTAGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCt                                                      >  2:2104012/1‑150 (MQ=255)
                                                                                                     tCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGt                                                 >  2:514687/1‑150 (MQ=255)
                                                                                                         gTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCaa                                             >  1:2794875/1‑150 (MQ=255)
                                                                                                          tttttACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAAc                                            <  1:89317/150‑1 (MQ=255)
                                                                                                          tttttACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAAc                                            <  1:2749134/150‑1 (MQ=255)
                                                                                                                      ttCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTc                                <  1:986489/150‑1 (MQ=255)
                                                                                                                      ttCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTc                                <  2:754219/150‑1 (MQ=255)
                                                                                                                        cAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCAc                              <  2:253222/150‑1 (MQ=255)
                                                                                                                        cAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCAc                              <  1:2606326/150‑1 (MQ=255)
                                                                                                                        cAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCAc                              >  2:1524803/1‑150 (MQ=255)
                                                                                                                                aGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACg                      <  1:2653572/150‑1 (MQ=255)
                                                                                                                                aGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACg                      <  1:2653510/150‑1 (MQ=255)
                                                                                                                                  cTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGaa                    <  1:1424107/150‑1 (MQ=255)
                                                                                                                                    ataAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGc                  >  1:2683032/1‑150 (MQ=255)
                                                                                                                                    ataAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGc                  >  2:238857/1‑150 (MQ=255)
                                                                                                                                       aaaaaaCCCGCTTCGGCGGGTTTTTTAGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATAAAACTTTGGAGAACAGCGGACGACGACGTGCTTTACGCAGACCGACATTCTAACGTTCAACCTGACGAGCGTCAAGAGTAACGAAGCCAg               >  1:794616/1‑150 (MQ=255)
                                                                                                                                        aaaaaCCCGCTTCGGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCCATTAACGTTTGGAGAACTGCGGACGACGACGTGCGTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGc              >  1:1189752/1‑150 (MQ=255)
                                                                                                                                        aaaaaCCCGCTTCGGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGc              >  1:774285/1‑150 (MQ=255)
                                                                                                                                            aCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTa          <  1:669217/150‑1 (MQ=255)
                                                                                                                                             cccGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGACAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTAc         <  1:15303/150‑1 (MQ=255)
                                                                                                                                                gccgAAGCGGGTTTTTTCGAAAATTGTTTTCTGTCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTAACGTTCAACCTGACGAGCGTCACGAGAAACGAAGCCAGCATTACGCa      >  1:2205366/1‑150 (MQ=255)
                                                                                                                                                 ccgAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAg     >  2:2836446/1‑150 (MQ=255)
                                                                                                                                                 ccgAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAg     <  1:2126903/150‑1 (MQ=255)
                                                                                                                                                    aaGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTc  <  2:897834/150‑1 (MQ=255)
                                                                                                                                                    aaGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCAGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTc  >  1:456952/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GAAAAAGAGATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTC  >  NC_000913/3377622‑3377919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: