Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,947,049 | A→T | 5.4% | intergenic (+17/+79) | trpT → / ← hdfR | tRNA‑Trp/flhDC operon transcriptional repressor |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,947,049 | 0 | A | T | 5.4% | 845.5 / 24.9 | 232 | intergenic (+17/+79) | trpT/hdfR | tRNA‑Trp/flhDC operon transcriptional repressor |
Reads supporting (aligned to +/- strand): ref base A (130/89); new base T (4/9); total (134/98) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.91e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 242 total aligned reads displayed.
CTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTATTTTGCA > NC_000913/3946900‑3947198 | tgcctgTCACGCAGGGGGTCGCGGGTTCGAGTCCCGGCCGTTCCGCCACCCTAATTAGGGGCGTAGTGCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGaa > 2:1365245/1‑150 (MQ=255) gcctgTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGaaa > 1:346499/1‑150 (MQ=255) ctgTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCCCCCGAATTAGGGGCGTAGGTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGGTGGGAGTTCGCGTCTCTCCGCCCCGGCCAGAAAGCATCCGTAGCGAAAGc > 1:1020039/1‑150 (MQ=38) ggggTCGCGGGTTCGAGTCCCGTCCGGTCCGCCACCCGAATTAGGGGCGTAGTTCAATAGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑ttttt < 1:175514/150‑1 (MQ=255) gggTCGCGGGTTCGAGTGCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCTGAAATCATCCTTAGCGAAAGCTAA‑GGA‑tttttt < 2:1549521/150‑1 (MQ=255) gggTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGATCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCAGCCACAAAACAACCATAGCGAAAGCTAA‑GGA‑tttttt > 1:2404910/1‑150 (MQ=255) gggTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑tttttt > 2:1327493/1‑150 (MQ=255) ggTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCATTAGCTTTCGCTAA‑GGA‑tgatttc < 2:326303/150‑7 (MQ=255) ggTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCATTAGCTTTCGCTAA‑GGA‑tgatttc < 2:326160/150‑7 (MQ=255) cgcgGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTAt < 2:2885637/150‑1 (MQ=255) gcgGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTGGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATc > 1:2916661/1‑150 (MQ=255) gcgGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATc > 1:2591856/1‑150 (MQ=255) gcgGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATc < 2:2405209/150‑1 (MQ=255) gcgGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGGTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATc > 1:727650/1‑150 (MQ=255) gcgGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAAATGGTAGAGAACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATAATACATAGCGAAAGCTAA‑AGA‑ATTTTTTTAta > 1:2644651/1‑149 (MQ=255) ggTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGa < 2:455812/150‑1 (MQ=255) ggTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGa < 2:570373/150‑1 (MQ=255) ttCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGaaa < 2:489829/150‑1 (MQ=255) ttCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGaaa > 1:1906723/1‑150 (MQ=255) cGAGTCCCGTCCGTTCCGCCACCCGAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAAACGGGTGTTGGGAGTTCGAGTCACTCCGCCCCTGCCAGAAATCAACCATAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATa > 1:1048962/1‑150 (MQ=255) gAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATaa < 2:2541768/150‑1 (MQ=255) aGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAAc < 2:1124986/150‑1 (MQ=255) aGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAAc > 1:1970169/1‑150 (MQ=255) tCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAAccc > 2:174003/1‑150 (MQ=255) tCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAAccc > 1:2764259/1‑150 (MQ=255) ccgtccgtTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCctc > 1:1114704/1‑150 (MQ=255) ccgtccgtTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCctc > 2:2478627/1‑150 (MQ=255) gtccgtTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCtctc < 1:1775698/150‑1 (MQ=255) tccgtTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTcc < 1:2425576/150‑1 (MQ=255) gtTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGaa < 1:142852/150‑1 (MQ=255) tCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGt < 1:588398/150‑1 (MQ=255) ccGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGCGTTCGAGTCTCTCCGCCCCTGCCAGACATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCGCTCCGAAGTa > 2:1244636/1‑150 (MQ=255) gCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTaaa > 1:375789/1‑150 (MQ=255) gCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTaaa < 2:942274/150‑1 (MQ=255) ccTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCtt < 1:604671/150‑1 (MQ=255) ccTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCtt > 1:1586222/1‑150 (MQ=255) aGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCgg < 1:924569/150‑1 (MQ=255) aGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCgg > 1:825596/1‑150 (MQ=255) aGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCgg > 1:2028519/1‑150 (MQ=255) aGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCgg < 1:318821/150‑1 (MQ=255) gggCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCCGCa > 1:1826609/1‑150 (MQ=255) gTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGATTTTTTTTTTTATTAAATAACCAACACCGAGGTAATTCCTGCTACCGGCATcc > 2:650165/1‑150 (MQ=255) tAGTTCAATTTGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGATATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGATGTAAATCCTTCTACCGGCATCCtt < 1:2723130/150‑1 (MQ=255) gTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgc < 1:2704559/150‑1 (MQ=255) ttCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcc > 1:643336/1‑150 (MQ=255) ttCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcc < 1:217487/150‑1 (MQ=255) cAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccag > 1:1456289/1‑150 (MQ=255) aaTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGACGTAAATCCTTCTACCGGCATCCTTgccagc > 1:450193/1‑150 (MQ=255) aaTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagc > 2:2694573/1‑150 (MQ=255) aaTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagc < 2:329380/150‑1 (MQ=255) ttGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagcca < 2:2180541/150‑1 (MQ=255) tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCa > 1:1084730/1‑150 (MQ=255) agCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCatat < 1:1314688/150‑1 (MQ=255) agCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCatat < 1:2898543/150‑1 (MQ=255) aCCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTaa < 1:1076021/150‑1 (MQ=255) gTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATaca < 1:2077/150‑1 (MQ=255) ctcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACt < 2:1008175/150‑1 (MQ=255) tcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACtt > 2:1437205/1‑150 (MQ=255) tcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACtt > 1:1295134/1‑150 (MQ=255) ccAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCCTTCATATTAATACACTTc > 2:366407/1‑150 (MQ=255) aaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATc < 2:2789558/150‑1 (MQ=255) aaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATcc < 2:2307319/150‑1 (MQ=255) aCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCa < 2:750454/150‑1 (MQ=255) ccGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCCTCCATCGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAg < 2:2079157/150‑1 (MQ=255) gggTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCa > 1:382337/1‑150 (MQ=255) gggTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTGCTACCGGCATCCTTGCCAGCCCTTCATATGAATACACTTCATCCAGcc > 2:89953/1‑149 (MQ=255) ggTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCAc > 1:878766/1‑150 (MQ=255) gtgtTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACg > 2:1826450/1‑150 (MQ=255) gtgtTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACg > 1:1390017/1‑150 (MQ=255) gtgtTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAGTCATATTAATACACTTCATCCAGCACg > 1:2521700/1‑150 (MQ=255) ttGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCTAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTGTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTa > 2:2050836/1‑150 (MQ=255) tGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTaa > 2:1480992/1‑150 (MQ=255) tGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTaa < 1:2257566/150‑1 (MQ=255) ggAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAAtt > 1:1863916/1‑150 (MQ=255) gTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTc < 2:1168391/150‑1 (MQ=255) gTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTc < 2:1953702/150‑1 (MQ=255) gTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTc < 2:1168432/150‑1 (MQ=255) tctctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGAt > 1:1563639/1‑150 (MQ=255) tctctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGAt > 1:167494/1‑150 (MQ=255) tctctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGAt > 1:1524472/1‑150 (MQ=255) tctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATcg > 1:1463704/1‑150 (MQ=255) ctcCGCCCCTGCCATTAATAATCCATAGCGTAAGCTAATGTT‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATcg < 1:650165/150‑1 (MQ=255) ctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATcgc > 1:414780/1‑150 (MQ=255) ccGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGa < 1:2772385/150‑1 (MQ=255) gCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAAt < 2:2487648/150‑1 (MQ=255) ccccTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATc > 1:1532038/1‑150 (MQ=255) ccccTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATc > 1:2203959/1‑150 (MQ=255) cTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAAc > 1:2761953/1‑150 (MQ=255) tGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACg > 2:1676152/1‑150 (MQ=255) aaTCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCAttttt < 1:1949807/150‑1 (MQ=255) atcatcCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTa > 1:2097763/1‑150 (MQ=255) aaaatcCTTAGCTTTCGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTa > 1:942864/4‑150 (MQ=255) atcCTTAGCTTTCGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCAttt > 1:485911/1‑144 (MQ=255) ccTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCt > 1:1363836/1‑150 (MQ=255) ttAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTAt > 1:2337828/1‑150 (MQ=255) ttAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCAAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTAt > 2:784801/1‑150 (MQ=255) cGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTATTTTg > 2:1891556/1‑150 (MQ=255) cGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTATTTTg > 2:2254676/1‑150 (MQ=255) aaaGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTATTTTGCa < 1:340653/150‑1 (MQ=255) aaaGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTATTTTGCa > 2:2794490/1‑150 (MQ=255) | CTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAA‑GGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTATTTTGCA > NC_000913/3946900‑3947198 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |