Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,947,051 A→C 5.2% intergenic (+19/+77) trpT → / ← hdfR tRNA‑Trp/flhDC operon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,947,0510AC 5.2% 856.0 / 26.0 232intergenic (+19/+77)trpT/hdfRtRNA‑Trp/flhDC operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (130/90);  new base C (4/8);  total (134/98)
Fisher's exact test for biased strand distribution p-value = 1.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 240 total aligned reads displayed.

GCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTATTTTGCA  >  NC_000913/3946902‑3947198
                                                                                                                                                     |                                                                                                                                                   
gcctgTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGaaa                                                                                                                                                     >  1:346499/1‑150 (MQ=255)
  ctgTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCCCCCGAATTAGGGGCGTAGGTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGGTGGGAGTTCGCGTCTCTCCGCCCCGGCCAGAAAGCATCCGTAGCGAAAGc                                                                                                                                                   >  1:1020039/1‑150 (MQ=38)
        cGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCTTTCGCTAAGGa                                                                                                                                             <  1:2711329/150‑1 (MQ=255)
           aGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAttt                                                                                                                                          >  2:1949807/1‑150 (MQ=255)
              gggTCGCGGGTTCGAGTGCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCTGAAATCATCCTTAGCGAAAGCTAAGGAtttttt                                                                                                                                       <  2:1549521/150‑1 (MQ=255)
              gggTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtttttt                                                                                                                                       >  2:1327523/1‑150 (MQ=255)
               ggTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCATTAGCTTTCGCTAAGGAtgatttc                                                                                                                                      <  2:326303/150‑7 (MQ=255)
               ggTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCATTAGCTTTCGCTAAGGAtgatttc                                                                                                                                      <  2:326242/150‑7 (MQ=255)
                  cgcgGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAt                                                                                                                                   <  2:2885637/150‑1 (MQ=255)
                   gcgGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAAATGGTAGAGAACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATAATACATAGCGAAAGCTAAAGAATTTTTTTAta                                                                                                                                  >  1:2644651/1‑149 (MQ=255)
                   gcgGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGGAGGTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCGAAGGATTTTTTTTATc                                                                                                                                  >  1:357746/1‑150 (MQ=255)
                    cgGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCt                                                                                                                                 >  2:1200665/1‑150 (MQ=255)
                      ggTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGa                                                                                                                               <  2:455812/150‑1 (MQ=255)
                      ggTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGa                                                                                                                               <  2:570373/150‑1 (MQ=255)
                        ttCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGaaa                                                                                                                             >  1:1906723/1‑150 (MQ=255)
                        ttCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGaaa                                                                                                                             <  2:489829/150‑1 (MQ=255)
                          cGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATa                                                                                                                           >  1:1703905/1‑150 (MQ=255)
                          cGAGTCCCGTCCGTTCCGCCACCCGAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAAACGGGTGTTGGGAGTTCGAGTCACTCCGCCCCTGCCAGAAATCAACCATAGCGAAAGCTAAGGATTTTTTTTATCTGAAATa                                                                                                                           >  1:1048962/1‑150 (MQ=255)
                            aGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAc                                                                                                                         >  1:1970169/1‑150 (MQ=255)
                             gTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAcc                                                                                                                        <  1:2414035/150‑1 (MQ=255)
                              tCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAccc                                                                                                                       >  2:174003/1‑150 (MQ=255)
                              tCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAccc                                                                                                                       >  1:2764087/1‑150 (MQ=255)
                                ccgtccgtTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctc                                                                                                                     >  2:2478627/1‑150 (MQ=255)
                                ccgtccgtTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctc                                                                                                                     >  1:1114704/1‑150 (MQ=255)
                                  gtccgtTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCtctc                                                                                                                   <  1:1775698/150‑1 (MQ=255)
                                      gtTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGaa                                                                                                               <  2:129009/150‑1 (MQ=255)
                                      gtTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGaa                                                                                                               <  2:2134110/150‑1 (MQ=255)
                                      gtTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGaa                                                                                                               <  2:1068431/150‑1 (MQ=255)
                                      gtTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGaa                                                                                                               <  1:142852/150‑1 (MQ=255)
                                        tCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGt                                                                                                             <  1:588438/150‑1 (MQ=255)
                                           gCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTaaa                                                                                                          >  1:872251/1‑150 (MQ=255)
                                           gCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTaaa                                                                                                          <  2:942274/150‑1 (MQ=255)
                                               cccTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCt                                                                                                      >  1:898364/1‑150 (MQ=255)
                                               cccTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCt                                                                                                      >  1:801374/1‑150 (MQ=255)
                                                ccTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCtt                                                                                                     <  1:1933612/150‑1 (MQ=255)
                                                ccTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCtt                                                                                                     >  1:1586222/1‑150 (MQ=255)
                                                       aGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCgg                                                                                              >  1:2028507/1‑150 (MQ=255)
                                                         gggCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCa                                                                                            >  1:340602/1‑150 (MQ=255)
                                                         gggCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCCGCa                                                                                            >  1:1826609/1‑150 (MQ=255)
                                                                   cAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccag                                                                                  >  1:1456289/1‑150 (MQ=255)
                                                                    aaTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagc                                                                                 >  1:450139/1‑150 (MQ=255)
                                                                    aaTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagc                                                                                 >  2:2694573/1‑150 (MQ=255)
                                                                    aaTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagc                                                                                 <  2:329411/150‑1 (MQ=255)
                                                                      ttGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagcca                                                                               <  2:2180541/150‑1 (MQ=255)
                                                                          tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCa                                                                           >  1:1084730/1‑150 (MQ=255)
                                                                             agCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCatat                                                                        <  1:1314688/150‑1 (MQ=255)
                                                                             agCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAGTCatat                                                                        >  1:2583156/1‑150 (MQ=255)
                                                                              gCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCCTTCATAtt                                                                       >  1:527651/1‑150 (MQ=255)
                                                                                aCCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTaa                                                                     <  1:1076021/150‑1 (MQ=255)
                                                                                  cGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTCGCGACAGCTAAGGATTTTTTTTATCTGAATTACCCCTCTCCGAAGTAAATCCTTCTACCGGCAGCCTTGCCCGCCATGCCTATTactc                                                                   >  1:707668/1‑147 (MQ=255)
                                                                                  cGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATa                                                                   <  1:940248/150‑1 (MQ=255)
                                                                                    gTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATaca                                                                 <  1:2077/150‑1 (MQ=255)
                                                                                       tcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACtt                                                              >  1:1295134/1‑150 (MQ=255)
                                                                                        ccAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCCTTCATATTAATACACTTc                                                             >  2:366407/1‑150 (MQ=255)
                                                                                           aaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATc                                                          <  1:2698900/150‑1 (MQ=255)
                                                                                            aaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATcc                                                         <  2:2307319/150‑1 (MQ=255)
                                                                                             aCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCa                                                        <  2:750454/150‑1 (MQ=255)
                                                                                                gggTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTGCTACCGGCATCCTTGCCAGCCCTTCATATGAATACACTTCATCCAGcc                                                     >  2:89953/1‑149 (MQ=255)
                                                                                                  gtgtTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACg                                                   >  1:1390017/1‑150 (MQ=255)
                                                                                                  gtgtTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAGTCATATTAATACACTTCATCCAGCACg                                                   >  1:2521700/1‑150 (MQ=255)
                                                                                                     ttGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCTAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTGTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTa                                                >  2:2050836/1‑150 (MQ=255)
                                                                                                     ttGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTa                                                >  1:382053/1‑150 (MQ=255)
                                                                                                      tGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTaa                                               >  2:1480992/1‑150 (MQ=255)
                                                                                                        ggAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAAtt                                             >  2:659891/1‑150 (MQ=255)
                                                                                                        ggAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAAtt                                             >  1:1863916/1‑150 (MQ=255)
                                                                                                        ggAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAAtt                                             >  2:241358/1‑150 (MQ=255)
                                                                                                           gTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTc                                          <  2:1168393/150‑1 (MQ=255)
                                                                                                            ttCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCa                                         >  1:628881/1‑150 (MQ=255)
                                                                                                             tCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAATCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCaa                                        <  1:2897897/150‑1 (MQ=255)
                                                                                                               gAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCaaaa                                      <  2:2292114/150‑1 (MQ=255)
                                                                                                                  tctctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGAt                                   >  1:167494/1‑150 (MQ=255)
                                                                                                                  tctctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGAt                                   >  1:1563639/1‑150 (MQ=255)
                                                                                                                   ctctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATACTTCTACCGGCATCCTTGCAAGCCATTCATATTAATACACTTCATCCAGAACGTTAATTTTCAAATGATc                                  >  2:1151216/1‑150 (MQ=255)
                                                                                                                    tctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATcg                                 <  1:649993/150‑1 (MQ=255)
                                                                                                                    tctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATcg                                 >  1:1463704/1‑150 (MQ=255)
                                                                                                                      tcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATcgcg                               >  1:1515905/1‑150 (MQ=255)
                                                                                                                      tcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGATAATTTTCAAAAGATcgcg                               >  1:309543/1‑150 (MQ=255)
                                                                                                                       ccGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGa                              <  1:2772385/150‑1 (MQ=255)
                                                                                                                         gCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAAt                            <  2:2487648/150‑1 (MQ=255)
                                                                                                                          ccccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATc                           >  1:2203959/1‑150 (MQ=255)
                                                                                                                          ccccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATc                           >  1:1532038/1‑150 (MQ=255)
                                                                                                                            ccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCaa                         >  1:400957/1‑150 (MQ=255)
                                                                                                                             cTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAAc                        >  1:2761953/1‑150 (MQ=255)
                                                                                                                              tGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACg                       >  2:182604/1‑150 (MQ=255)
                                                                                                                              tGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACg                       >  2:1676152/1‑150 (MQ=255)
                                                                                                                                  aGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCAtt                   >  1:2901915/1‑150 (MQ=255)
                                                                                                                                     aaTCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCAttttt                <  2:1407411/150‑1 (MQ=255)
                                                                                                                                      atcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTa               >  1:2097763/1‑150 (MQ=255)
                                                                                                                                      aaaatcCTTAGCTTTCGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTa               >  2:2549839/4‑150 (MQ=255)
                                                                                                                                         atcCTTAGCTTTCGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCAttt                  >  1:485810/1‑144 (MQ=255)
                                                                                                                                         atcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCCTTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGTTCGCGAAGCAACGCATTTGTATCg            >  1:2146441/1‑150 (MQ=255)
                                                                                                                                          tcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGc           >  1:992501/1‑150 (MQ=255)
                                                                                                                                           ccTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCt          >  1:1363836/1‑150 (MQ=255)
                                                                                                                                             ttAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTAt        >  1:2337828/1‑150 (MQ=255)
                                                                                                                                             ttAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTAt        >  1:2337840/1‑150 (MQ=255)
                                                                                                                                             ttAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCAAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTAt        >  2:784801/1‑150 (MQ=255)
                                                                                                                                               aGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTAttt      <  1:2254375/150‑1 (MQ=255)
                                                                                                                                                 cGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTATTTTg    >  2:1891556/1‑150 (MQ=255)
                                                                                                                                                   aaaGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTATTTTGCa  >  2:2794490/1‑150 (MQ=255)
                                                                                                                                                   aaaGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTATTTTGCa  >  2:2794450/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTATTTTGCA  >  NC_000913/3946902‑3947198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: