Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 779,660 C→G 5.2% P21P (CCC→CCG ybgF → periplasmic TolA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913779,6600CG 5.2% 642.5 / 20.8 173P21P (CCC→CCGybgFperiplasmic TolA‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (73/91);  new base G (4/5);  total (77/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 189 total aligned reads displayed.

GGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTG  >  NC_000913/779511‑779808
                                                                                                                                                     |                                                                                                                                                    
 gTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCt                                                                                                                                                     >  2:174126/1‑150 (MQ=255)
 gTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGGTGGTATAGCGGCCCCCt                                                                                                                                                     >  2:1817319/1‑150 (MQ=255)
  tAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTg                                                                                                                                                    <  2:282155/150‑1 (MQ=255)
   aaaGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTgg                                                                                                                                                   <  1:1021356/150‑1 (MQ=255)
    aaGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTggg                                                                                                                                                  <  1:2112298/150‑1 (MQ=255)
          aaCCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAACACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCAGGGGGCCGCta                                                                                                                                            <  1:780136/150‑2 (MQ=255)
           aCCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCatt                                                                                                                                           <  2:2659902/150‑1 (MQ=255)
            ccTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCAGGGGGCCGCtata                                                                                                                                          <  2:9803/150‑4 (MQ=255)
                 aGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCa                                                                                                                                     <  2:1123767/150‑1 (MQ=255)
                   tACTGGGTCATGACGAAGCGGCATACTCAAAAAACCGTCGTGCGGTACTGGTTTACTAAGATAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAgg                                                                                                                                   <  1:675683/150‑1 (MQ=255)
                      tGGGTCATGACGAAGCGGCATACTCCAAAACCCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCAc                                                                                                                                <  1:1217714/150‑1 (MQ=255)
                      tGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCAc                                                                                                                                <  1:273008/150‑1 (MQ=255)
                          tCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAAt                                                                                                                            >  1:2828246/1‑150 (MQ=255)
                           cATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACAGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATc                                                                                                                           >  1:326072/1‑150 (MQ=255)
                             tGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCag                                                                                                                         >  1:1841612/1‑150 (MQ=255)
                                 gAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGTGCAGTAACTTCAGACATCAACTAGTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCagtagt                                                                                                                     >  1:1065698/1‑150 (MQ=255)
                                 gAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCagtagt                                                                                                                     <  2:220485/150‑1 (MQ=255)
                                  aaGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGACTTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAgtg                                                                                                                    <  1:1262509/150‑1 (MQ=255)
                                   aGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAgtgt                                                                                                                   <  1:1412462/150‑1 (MQ=255)
                                    gCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTc                                                                                                                  <  1:1819167/150‑1 (MQ=255)
                                    gCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTc                                                                                                                  >  2:1683553/1‑150 (MQ=255)
                                      ggCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCgg                                                                                                                >  1:1790834/1‑150 (MQ=255)
                                         aTACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTc                                                                                                             >  2:1876851/1‑150 (MQ=255)
                                            cTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCTTGAGCAGTAACTTCATACATCAAGTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCTCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAgg                                                                                                          <  1:1219361/150‑1 (MQ=255)
                                            cTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAgg                                                                                                          >  1:989860/1‑150 (MQ=255)
                                             tCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGc                                                                                                         >  2:290867/1‑150 (MQ=255)
                                               cAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCCCCCATCAGTAGTGTCGGCGCAGGCTc                                                                                                       >  1:2727610/1‑150 (MQ=255)
                                               cAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTc                                                                                                       >  1:269203/1‑150 (MQ=255)
                                               cAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCGTTTGCTCAGGCACCAATCAGGAGTGTCGGCGCAGGCTc                                                                                                       >  1:1273857/1‑150 (MQ=255)
                                                aacaaCCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCACCTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCg                                                                                                      <  2:1794795/147‑1 (MQ=255)
                                                 aaaaCCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCgg                                                                                                     >  2:2791557/1‑150 (MQ=255)
                                                  aaaCCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGt                                                                                                    <  2:1644884/150‑1 (MQ=255)
                                                   caCCGTCGTGCGGTACTGGTTTACTAAGAGAAGTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTc                                                                                                   <  1:1204684/149‑1 (MQ=255)
                                                   aaCCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTc                                                                                                   <  1:1374989/150‑1 (MQ=255)
                                                      cgtcgtGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGaa                                                                                                <  1:555454/150‑1 (MQ=255)
                                                        tcgtGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGa                                                                                              >  2:1731126/1‑150 (MQ=255)
                                                        tcgtGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGa                                                                                              >  2:1577483/1‑150 (MQ=255)
                                                        tcgtGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGa                                                                                              >  1:1177120/1‑150 (MQ=255)
                                                          gtGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGACGAcc                                                                                            <  2:544214/150‑1 (MQ=255)
                                                          gtGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGAcc                                                                                            <  2:316549/150‑1 (MQ=255)
                                                          gtGCGGTACTGGTGTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTAGTGAGTCTGTCGTTACTGGGTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCACTCAGTAGTGTCGGCTCAGGCTCGGTCGAAGAcc                                                                                            <  2:886128/150‑1 (MQ=255)
                                                              ggTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGt                                                                                        >  2:2364206/1‑150 (MQ=255)
                                                              ggTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGt                                                                                        <  1:1500492/150‑1 (MQ=255)
                                                                tACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCa                                                                                      <  1:1811196/150‑1 (MQ=255)
                                                                     gTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCaa                                                                                 >  2:1431647/1‑150 (MQ=255)
                                                                     gTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTCGTTGGTATAGAGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCaa                                                                                 >  2:2022271/1‑150 (MQ=255)
                                                                          cTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGa                                                                            <  1:870370/150‑1 (MQ=255)
                                                                          cTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGa                                                                            <  2:2244409/150‑1 (MQ=255)
                                                                           tAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAg                                                                           <  1:2940255/150‑1 (MQ=255)
                                                                            aaGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGc                                                                          >  1:1820708/1‑150 (MQ=255)
                                                                            aaGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGc                                                                          >  2:2321573/1‑150 (MQ=255)
                                                                            aaGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGc                                                                          >  1:1155680/1‑150 (MQ=255)
                                                                             agagAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCg                                                                         >  2:1938868/1‑150 (MQ=255)
                                                                             agagAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCGTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCg                                                                         >  1:161466/1‑150 (MQ=255)
                                                                                gaATTGCATGAGCAGTAACTTCAGTCATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGATGACCGCGTCACTCAACTTGAGCGTAt                                                                      <  2:2903139/150‑1 (MQ=255)
                                                                                gaATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTAt                                                                      <  2:2045931/150‑1 (MQ=255)
                                                                                gaATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTAt                                                                      >  2:1361180/1‑150 (MQ=255)
                                                                                 aaTTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTAtt                                                                     <  1:791421/150‑1 (MQ=255)
                                                                                  aTTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTAttt                                                                    <  1:167495/150‑1 (MQ=255)
                                                                                   ttGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTc                                                                   <  1:2180000/150‑1 (MQ=255)
                                                                                   ttGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTc                                                                   <  1:2211519/150‑1 (MQ=255)
                                                                                      cATGAGCAGTAACTTCAGACCTCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTaa                                                                <  1:372156/150‑1 (MQ=255)
                                                                                        tGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCGCAGGCTCGGTCGAAGACCGCGTCCCTCCACTTGAGCGTATTTCTAATg                                                              >  2:2234377/1‑150 (MQ=255)
                                                                                               tAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCAcag                                                       >  2:2350155/1‑150 (MQ=255)
                                                                                               tAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCAcag                                                       >  2:601579/1‑150 (MQ=255)
                                                                                                 aCTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCAcagcc                                                     >  2:2483866/1‑150 (MQ=255)
                                                                                                    tCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCAcagccagc                                                  <  2:2660255/150‑1 (MQ=255)
                                                                                                      aGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCtt                                                <  2:2855389/150‑1 (MQ=255)
                                                                                                       gACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCttt                                               <  2:2675080/150‑1 (MQ=255)
                                                                                                       gACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCttt                                               <  2:1841612/150‑1 (MQ=255)
                                                                                                       gACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCttt                                               <  2:130764/150‑1 (MQ=255)
                                                                                                          aTCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTaa                                            <  2:1048338/150‑1 (MQ=255)
                                                                                                             aaCTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACATGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAAccc                                         >  1:1721051/1‑150 (MQ=255)
                                                                                                             aaCTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAAAAAGTAGTGTCGGCGCAGGCTCGGTCGAAGACCGCGTAACACAAAAATAGCCTATTTATAATACACACAACCACCATTTAAccc                                         >  1:2114352/1‑150 (MQ=255)
                                                                                                             aaCTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCGTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACACCCAGCTGTTAAacc                                         >  1:2075851/1‑150 (MQ=255)
                                                                                                                 aTTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACt                                     <  2:2487585/150‑1 (MQ=255)
                                                                                                                      gTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGc                                <  1:1250798/150‑1 (MQ=255)
                                                                                                                               tACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACttt                       <  1:60958/150‑1 (MQ=255)
                                                                                                                                  tggttggtATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACGTTCTg                    >  2:763657/1‑150 (MQ=255)
                                                                                                                                  tggttggtATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCAAGCAACAACTTTCTg                    <  1:2791557/150‑1 (MQ=255)
                                                                                                                                   ggttggtATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGa                   <  2:499459/150‑1 (MQ=255)
                                                                                                                                   ggttggtATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGa                   <  2:499539/150‑1 (MQ=255)
                                                                                                                                       ggtATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATaat               <  2:2570849/150‑1 (MQ=255)
                                                                                                                                       ggtATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATaat               <  1:459640/150‑1 (MQ=255)
                                                                                                                                         tataGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATaatca             <  2:1372705/150‑1 (MQ=255)
                                                                                                                                          aaaGCGGCCCAGGGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACGTTCTGATaatcaa            >  2:697119/3‑150 (MQ=255)
                                                                                                                                           taGCGGCCCCCTGGGCCGCGTTTTCTCAGGCCCCAATCAGTAGTGTAGGCTCAGGCTCGGTCGCATACCCCGTCACTCAACATGAGCGTATTTATAATTCTCAAACCCAGCTTTTAACCCAACTCCAGCAACAACATTATGATaataaaa           >  1:2878957/1‑149 (MQ=255)
                                                                                                                                           aaGCGGCCCAGGGGGCCGCTTTTGCGCAGGCACCAATCAGTAGTGTCGGCTCCGGCTCGGTCGAAGCCCGCGTCACTCACCGTGAGCGTATTTCTAATGCTCCCAGCCAGCTTTTAACCCAACTCCCGCACCAACTGGCGGATactcact           >  2:673165/2‑148 (MQ=255)
                                                                                                                                             gCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATcc         <  1:174126/150‑1 (MQ=255)
                                                                                                                                             gCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATcc         <  1:173924/150‑1 (MQ=255)
                                                                                                                                              cGGCCCCCTGGGCCGCGTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCACCAACTGTCTGATAATCAATCCg        >  2:2261002/1‑150 (MQ=255)
                                                                                                                                               ggCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGa       <  2:326072/150‑1 (MQ=255)
                                                                                                                                                    ccTGGGCTGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTg  <  1:1011148/150‑1 (MQ=255)
                                                                                                                                                    ccTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTg  <  1:1304456/150‑1 (MQ=255)
                                                                                                                                                    ccTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTg  <  1:776824/150‑1 (MQ=255)
                                                                                                                                                    ccTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTg  <  1:749123/150‑1 (MQ=255)
                                                                                                                                                    ccTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTg  <  1:301015/150‑1 (MQ=255)
                                                                                                                                                    ccTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTg  <  1:2313047/150‑1 (MQ=255)
                                                                                                                                                    ccTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTg  <  1:222002/150‑1 (MQ=255)
                                                                                                                                                    ccTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTg  <  1:2104865/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAGCCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTG  >  NC_000913/779511‑779808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: