Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,473,927 C→A 100% A43S (GCC→TCC)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,473,9270CA100.0% 849.6 / ‑6.7 221A43S (GCC→TCC) bdcAc‑di‑GMP‑binding biofilm dispersal mediator protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (100/120);  total (101/120)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 221 total aligned reads displayed.

GGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTA  >  NC_000913/4473781‑4474073
                                                                                                                                                  |                                                                                                                                                  
ggCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATc                                                                                                                                                 <  1:648696/150‑1 (MQ=255)
   cTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCACTGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCttt                                                                                                                                              <  2:1816087/150‑1 (MQ=255)
   cTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCttt                                                                                                                                              <  2:1311411/150‑1 (MQ=255)
   cTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCttt                                                                                                                                              <  1:2724987/150‑1 (MQ=255)
   cTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCttt                                                                                                                                              <  2:324444/150‑1 (MQ=255)
    tCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTc                                                                                                                                             <  2:2512574/150‑1 (MQ=255)
    tCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTc                                                                                                                                             >  1:919920/1‑150 (MQ=255)
    tCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTc                                                                                                                                             <  2:1426455/150‑1 (MQ=255)
    tCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTc                                                                                                                                             <  2:1916552/150‑1 (MQ=255)
    tCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAAGGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTc                                                                                                                                             <  2:2214163/150‑1 (MQ=255)
      gCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGa                                                                                                                                           >  1:2186203/1‑150 (MQ=255)
      gCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGa                                                                                                                                           >  2:1884571/1‑150 (MQ=255)
       ccAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGAc                                                                                                                                          <  2:2451749/150‑1 (MQ=255)
       ccAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGAc                                                                                                                                          <  1:2114459/150‑1 (MQ=255)
        cAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGAcc                                                                                                                                         >  1:2800672/1‑150 (MQ=255)
        cAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGAcc                                                                                                                                         <  1:2467832/150‑1 (MQ=255)
        cAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGAcc                                                                                                                                         >  1:2593850/1‑150 (MQ=255)
           aGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCg                                                                                                                                      <  2:1695796/150‑1 (MQ=255)
              cGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCAt                                                                                                                                   <  2:781853/150‑1 (MQ=255)
                 cAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAgg                                                                                                                                >  1:894311/1‑150 (MQ=255)
                 cAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAgg                                                                                                                                >  1:2421996/1‑150 (MQ=255)
                  aaTACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGt                                                                                                                               <  2:1115584/150‑1 (MQ=255)
                      ccTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAAt                                                                                                                           <  2:40287/150‑1 (MQ=255)
                      ccTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAAt                                                                                                                           >  2:1604963/1‑150 (MQ=255)
                       cTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATc                                                                                                                          >  1:737716/1‑150 (MQ=255)
                       cTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATc                                                                                                                          >  2:1365917/1‑150 (MQ=255)
                       cTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATc                                                                                                                          >  1:791613/1‑150 (MQ=255)
                       cTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATc                                                                                                                          >  1:336389/1‑150 (MQ=255)
                       cTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATc                                                                                                                          >  1:1537576/1‑150 (MQ=255)
                       cTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATc                                                                                                                          >  1:2487649/1‑150 (MQ=255)
                       cTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATc                                                                                                                          >  2:238611/1‑150 (MQ=255)
                       cTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATc                                                                                                                          >  2:2007939/1‑150 (MQ=255)
                       cTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATc                                                                                                                          >  2:1905606/1‑150 (MQ=255)
                         gCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGt                                                                                                                        >  2:1257082/1‑150 (MQ=255)
                          cATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTa                                                                                                                       <  2:1968118/150‑1 (MQ=255)
                          cATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTa                                                                                                                       <  2:2040567/150‑1 (MQ=255)
                          cATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTa                                                                                                                       <  2:1714743/150‑1 (MQ=255)
                          cATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTa                                                                                                                       <  2:608332/150‑1 (MQ=255)
                            tttACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACa                                                                                                                     >  2:2201896/1‑150 (MQ=255)
                                 caccaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGc                                                                                                                <  1:474073/150‑1 (MQ=255)
                                  accaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGcc                                                                                                               >  1:2631726/1‑150 (MQ=255)
                                     aGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCa                                                                                                            <  1:2349040/150‑1 (MQ=255)
                                                 gcgcCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGaaa                                                                                                <  1:2645854/150‑1 (MQ=255)
                                                 gcgcCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGaaa                                                                                                <  2:787148/150‑1 (MQ=255)
                                                 gcgcCGCTCTTACGAACGACACCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGaaa                                                                                                <  1:1076033/150‑1 (MQ=255)
                                                  cgccgcTCTTACGAACCACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATATTTAGACCCCCCAAAGGTGAATCGTACATTGGCCCCAAACGTGACGAAac                                                                                               >  1:1318426/1‑150 (MQ=255)
                                                   gccgcTCTTACGAACGACATCAATGACCGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACCTTGGCCCCATCGGTGACGAAacg                                                                                              >  1:2917886/1‑150 (MQ=255)
                                                   gccgcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATAATGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCATTCGACCCCGCAAAGGTGAATCGTACATTGGCCCCATCGGTGACGAAacg                                                                                              >  1:2857642/1‑150 (MQ=255)
                                                    ccgcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAacga                                                                                             <  2:2103864/150‑1 (MQ=255)
                                                          ttACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACg                                                                                       <  1:1884571/150‑1 (MQ=255)
                                                              gaacgaCATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAg                                                                                   >  1:2673705/1‑150 (MQ=255)
                                                                acgaCATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCg                                                                                 <  1:2926140/150‑1 (MQ=255)
                                                                acgaCATCAATGACAGCGCCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCg                                                                                 <  1:2090038/150‑1 (MQ=255)
                                                                   aCATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCa                                                                              <  2:2796267/150‑1 (MQ=255)
                                                                    cATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCAc                                                                             <  2:2666679/150‑1 (MQ=255)
                                                                     aTCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCAcc                                                                            >  2:851088/1‑150 (MQ=255)
                                                                     aTCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCAcc                                                                            <  1:2756511/150‑1 (MQ=255)
                                                                       cAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGa                                                                          >  1:1882194/1‑150 (MQ=255)
                                                                       cAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGa                                                                          >  1:2083472/1‑150 (MQ=255)
                                                                        aaTGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGAt                                                                         <  1:1039796/150‑1 (MQ=255)
                                                                        aaTGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGAt                                                                         >  1:1623901/1‑150 (MQ=255)
                                                                          tGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATAc                                                                       <  1:2815927/150‑1 (MQ=255)
                                                                          tGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATAc                                                                       <  1:178197/150‑1 (MQ=255)
                                                                              aGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATATCGGCACCGATACCACg                                                                   >  1:524819/1‑150 (MQ=255)
                                                                               gCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACAATGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGa                                                                  >  2:2082420/1‑150 (MQ=255)
                                                                                 gTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACt                                                                >  1:439152/1‑150 (MQ=255)
                                                                                   ctctGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGc                                                              >  2:1639255/1‑150 (MQ=255)
                                                                                      tGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCAc                                                           >  1:2708603/1‑150 (MQ=255)
                                                                                      tGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCAc                                                           >  1:582983/1‑150 (MQ=255)
                                                                                          aGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAg                                                       <  2:2620210/150‑1 (MQ=255)
                                                                                            cACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGa                                                     <  1:409490/150‑1 (MQ=255)
                                                                                             aCTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTTTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACAATTGCCCCAACGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGAt                                                    >  1:845570/1‑150 (MQ=255)
                                                                                             aCTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGAt                                                    >  1:2313212/1‑150 (MQ=255)
                                                                                                  cTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGaa                                               <  1:1907295/150‑1 (MQ=255)
                                                                                                   tgtgAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAAc                                              <  1:2543613/150‑1 (MQ=255)
                                                                                                    gtgAATACTGCTGTCGCTCCAGTCTCTTTTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCAACGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCCAGGATGAGAACt                                             >  1:1131501/1‑150 (MQ=255)
                                                                                                       aaTACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTc                                          <  1:381669/150‑1 (MQ=255)
                                                                                                              ctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTg                                   >  2:882653/1‑150 (MQ=255)
                                                                                                                   gCTCCAGTCTCTTGTGCCAGGCGTTTATCGGAATCTTTCGACCCCGCATAGGTGAATAGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCAACGATACAACGACTGCCACCGAGGATGATAACTGTCTTACCTGTaaaa                              >  1:2151795/1‑150 (MQ=255)
                                                                                                                   gCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTaaaa                              >  2:564164/1‑150 (MQ=255)
                                                                                                                        aGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGcc                         >  2:2368715/1‑150 (MQ=255)
                                                                                                                        aGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGcc                         >  2:369293/1‑150 (MQ=255)
                                                                                                                          tctcTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCGCGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCa                       >  2:2693292/1‑150 (MQ=255)
                                                                                                                          tctcTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCa                       >  1:1457430/1‑150 (MQ=255)
                                                                                                                          tctcTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCa                       >  1:395120/1‑150 (MQ=255)
                                                                                                                            tctTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGATAACTGTCTTACCTGTAAAAGCGCCCATa                     >  1:2234621/1‑150 (MQ=255)
                                                                                                                             ctTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATaa                    >  2:2291129/1‑150 (MQ=255)
                                                                                                                              ttGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAg                   >  2:2144769/1‑150 (MQ=255)
                                                                                                                                 tgCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGAc                <  2:2857700/150‑1 (MQ=255)
                                                                                                                                  gCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACt               <  1:2384009/150‑1 (MQ=255)
                                                                                                                                   ccAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTc              <  2:1623901/150‑1 (MQ=255)
                                                                                                                                    cAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTcc             <  2:1092549/150‑1 (MQ=255)
                                                                                                                                       gCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTg          >  1:2126338/1‑150 (MQ=255)
                                                                                                                                       gCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTg          <  1:1111057/150‑1 (MQ=255)
                                                                                                                                         gTTTAGCGGAATCTTTCGACCCCGCATAGGTAAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGAt        >  1:196697/1‑150 (MQ=255)
                                                                                                                                            tAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATtta     <  2:1314709/150‑1 (MQ=255)
                                                                                                                                             aGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATttat    <  1:1631956/150‑1 (MQ=255)
                                                                                                                                              gCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATttatt   <  2:723901/150‑1 (MQ=255)
                                                                                                                                              gCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATttatt   <  2:2317091/150‑1 (MQ=255)
                                                                                                                                              gCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATttatt   <  1:1049547/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTA  >  NC_000913/4473781‑4474073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: