BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	781,237	C→G	5.4%	intergenic (+15/‑132)	lysY → / → lysZ	tRNA‑Lys/tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	781,237	0	C	G	5.4%	428.1 / 14.1	131	intergenic (+15/‑132)	lysY/lysZ	tRNA‑Lys/tRNA‑Lys
Reads supporting (aligned to +/- strand): ref base C (77/45); new base G (4/3); total (83/48)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.76e-01

Only 100 of 131 total aligned reads displayed.

CACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTT  >  NC_000913/781092‑781385

cACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAg                                                                                                                                                  >  1:2002630/1‑150 (MQ=40)

    tCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATAAAACTCAGATGt                                                                                                                                              >  1:1009687/1‑150 (MQ=255)

     cccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGtt                                                                                                                                             >  2:2362332/1‑150 (MQ=255)

       cTTACCTGGAGGGGGTCGGCGGTTCGTTCACTTCATCACAGACCTGTGGTGTCGTTATCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCTGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCTGAGTTTGATGTTaa                                                                                                                                           <  1:2862816/150‑1 (MQ=11)

         tACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTAAACATCAAACTAAGAAGTTAAGc                                                                                                                                         >  2:437478/1‑150 (MQ=255)

             aggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGa                                                                                                                                     >  2:375420/1‑150 (MQ=255)

              ggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGaa                                                                                                                                    >  2:542152/1‑150 (MQ=255)

              ggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGaa                                                                                                                                    >  1:2831999/1‑150 (MQ=255)

                    ggTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATaa                                                                                                                              <  1:1747816/150‑1 (MQ=255)

                        ggcggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGtt                                                                                                                          >  2:1227249/1‑150 (MQ=255)

                         gcggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTg                                                                                                                         >  2:2058585/1‑150 (MQ=255)

                          cggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgc                                                                                                                        <  1:263485/150‑1 (MQ=255)

                             ttCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAGTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGcc                                                                                                                     >  1:2038188/1‑150 (MQ=255)

                               cGATCCCGGCATCCCCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAg                                                                                                                   <  2:2349396/150‑1 (MQ=255)

                                 aTCCCGTCATCACCCACCACTCGGGTCGTTATATAAGTTTGTAGATCCGTTGACTTTTAATAAATATGTCGCAGGTTCGAAACCTGCACGACCCAACAGTTTTAACATCAAACTCAGATCTTAAGCGTGAAGGATAACGATGCGCCAGCa                                                                                                                 >  1:2702706/1‑150 (MQ=14)

                                 aTCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATTTTAAGCGTGAAGGATAACGTTGCGCCAGCa                                                                                                                 >  1:2001908/1‑150 (MQ=255)

                                 aTCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCAAGACCCACCACGATTAAAATGAAACTCAGAGGTTAAGCGGGCGGGAGACCGTTGCGCCAGCa                                                                                                                 >  1:938263/1‑150 (MQ=14)

                                  tCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAATCTCAGATGTTAACCGTGAAGTAAAACGTTGCGCCAGcta                                                                                                                >  2:1830220/1‑148 (MQ=255)

                                  tCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACGCAGATGTGAAGCGTGAAGGATAACGTTGCGCAAGCac                                                                                                                >  2:85770/1‑149 (MQ=255)

                                   cccGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAAc                                                                                                               >  2:1327940/1‑150 (MQ=255)

                                          tcaCCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCga                                                                                                        >  2:292581/1‑149 (MQ=255)

                                           cacccaccACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTGAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTa                                                                                                       <  2:2675882/150‑1 (MQ=255)

                                             cccaccAC‑CGGGTCGTCAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGcc                                                                                                                     >  2:2449301/1‑133 (MQ=255)

                                             cccaccAC‑CGGGTCGTCAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGcc                                                                                                                     <  1:2449301/133‑1 (MQ=255)

                                                accacTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGgcg                                                                                                  >  2:1077109/1‑150 (MQ=255)

                                                          tCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGagcgaagcga                                                                                        <  2:1646648/150‑1 (MQ=255)

                                                          tCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGCTCGCAGGTTCGAAACCTGCACGACCCACCAGTTTAAACATCAAACACAGATGTGAATCGTGAAGGATAACGTTGCGCCAGCACCGGCCCGTCGGGCGggcgaagcga                                                                                        >  2:914195/1‑150 (MQ=11)

                                                          gcGTTAGCTCAGTTGGTAGAGCACTACACCATTTATCTAGTGCTCGCAGTTCCGAATCCTGCTCCACCCACCAGTTCTAACATCCAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGagcgaagcga                                                                                        <  2:2034377/149‑1 (MQ=14)

                                                           cGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAg                                                                                       <  2:1935032/150‑1 (MQ=255)

                                                            gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGt                                                                                      >  2:386322/1‑150 (MQ=255)

                                                            gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGt                                                                                      >  2:723526/1‑150 (MQ=255)

                                                             ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTc                                                                                     <  2:1636280/150‑1 (MQ=255)

                                                                gCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATc                                                                                  <  2:1703571/150‑1 (MQ=255)

                                                                      ttGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCAc                                                                            >  1:737808/1‑150 (MQ=255)

                                                                      ttGGTAGAGCAGTTGACTTTTAATAAAATTGTCGCAGGTTCGAAACCTGCACGACCCACCAGTTTTAAAATCAAAATCAGATTTTAAGCTTGAAGGATAACTTTTCGCCAGCAAAGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCAc                                                                            >  1:1401773/1‑150 (MQ=14)

                                                                         ttAGAGCAGTTGACTATTAATCTTTTGGTCGCAGGTGCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGAc                                                                         <  2:251212/149‑1 (MQ=255)

                                                                         gTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGa                                                                                                                                                 <  2:2589352/78‑1 (MQ=255)

                                                                         gTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGa                                                                                                                                                 <  2:2589362/78‑1 (MQ=255)

                                                                         gTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGa                                                                                                                                                 <  2:2730639/78‑1 (MQ=255)

                                                                         gTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGAc                                                                         >  2:563054/1‑150 (MQ=255)

                                                                         gTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACACAGa                                                                                                                                                 >  1:2589362/1‑78 (MQ=21)

                                                                                  ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATc                                                                >  1:1224894/1‑150 (MQ=255)

                                                                                   tGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCt                                                               >  1:335686/1‑150 (MQ=255)

                                                                                     aattttAAACAATTGGTCGCAGGTTCTAATCCTGCACGACCCACCATTTTTAAAATAAATCTCTTTTTTTAAGCGTTAAGGTTATCTTTGCGCCAGAAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTa                                                             <  1:974198/148‑1 (MQ=14)

                                                                                      cTTTTAATCAATTGGTCGCTGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaa                                                            <  1:2366970/150‑1 (MQ=255)

                                                                                      cTTTTAATATTTTGGTCGCAGGTTCTAATCCTGCACGACCCACCTTTTTTAACATAAAACTCAGATTTTATGCGTGAAGTATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaa                                                            <  1:2366983/150‑1 (MQ=14)

                                                                                       ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                                           >  1:2742179/1‑150 (MQ=255)

                                                                                       ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                                           >  2:1078821/1‑150 (MQ=255)

                                                                                         ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGa                                                         <  2:2831999/150‑1 (MQ=255)

                                                                                            aTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTg                                                      <  2:1404816/150‑1 (MQ=255)

                                                                                              cAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGTTGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTggt                                                    <  1:1506028/150‑2 (MQ=255)

                                                                                                aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGAGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGAGTGGccc                                                  >  1:688437/1‑150 (MQ=255)

                                                                                                aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGAATAAACATCAAACGCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCTCGTCCCACCGGTCTTATGGTTTGGccc                                                  >  1:2215352/1‑150 (MQ=14)

                                                                                                 ttGGTCGCTTTTTCTAATCCTGCACGACCCACCTGTTTTAACATCAAACTCAGTTTTTAAGCGTTAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGcccc                                                 <  1:1327940/150‑1 (MQ=17)

                                                                                                 ttGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGcccc                                                 >  1:1371108/1‑150 (MQ=255)

                                                                                                     tCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGACAGCAACGGCCCGTAGGGCGAGCGTAGCGAGTCAGCCAGCACGACACACCACTCTGAGATATTGTCCCCGAgg                                             >  1:1369275/1‑149 (MQ=14)

                                                                                                         aGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTaaaa                                         <  2:2789981/150‑1 (MQ=255)

                                                                                                          ggTTCGAATCCTGCACGACCCACCTGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCTGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTAGCCCCGAGTaaaaa                                        <  2:1107942/150‑1 (MQ=255)

                                                                                                          ggTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTAGCCCCGAGTaaaaa                                        <  2:41866/150‑1 (MQ=255)

                                                                                                           gTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAAGCGTAAAGATTGGCCCCGAGTaaaaag                                       >  2:260523/1‑149 (MQ=255)

                                                                                                               gAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCttt                                   <  2:2922256/150‑1 (MQ=14)

                                                                                                               gAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCttt                                   <  2:1197698/150‑1 (MQ=255)

                                                                                                                aaTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCGCCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTc                                  >  1:288757/1‑150 (MQ=255)

                                                                                                                aaTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTc                                  >  2:1586261/1‑150 (MQ=255)

                                                                                                                 aTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                 >  1:531564/1‑150 (MQ=255)

                                                                                                                 aTCCTGCACGACCCACCAGTTTAAAAATCAAACTCAGAAGTTCAGCGTGAAGGATAACTTTGCGCCAGAAACGGCCCGTAGGGCGAGAGAAGCGAGTCATCCTGCACGACCCACCAATCTGAAAGAGTGGCCCCGAGGAAAAGTCTATcg                                 >  1:2729295/1‑149 (MQ=17)

                                                                                                                    cTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGTCCCCGAGTAAAAATCTTTCAGGt                              >  2:1887861/1‑150 (MQ=255)

                                                                                                                     tGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTa                             >  1:2654791/1‑150 (MQ=255)

                                                                                                                      gCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTaa                            >  1:1140216/1‑150 (MQ=255)

                                                                                                                      gCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTaa                            >  1:1140229/1‑150 (MQ=255)

                                                                                                                       cACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGAACCGTGTAAATATCTTTCAGGTAac                           >  2:1898075/1‑150 (MQ=14)

                                                                                                                       cACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGACAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAac                           >  2:1694201/1‑150 (MQ=17)

                                                                                                                         cGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAacac                         <  1:1078821/150‑1 (MQ=255)

                                                                                                                          gACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACAcc                        >  2:1422781/1‑150 (MQ=255)

                                                                                                                           aCCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACAccc                       >  1:2884490/1‑150 (MQ=255)

                                                                                                                           aCCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACAccc                       >  1:2884868/1‑150 (MQ=255)

                                                                                                                             ccaccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGt                     >  1:818819/1‑150 (MQ=255)

                                                                                                                             ccaccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCCGATCAAACATCACCAACCACCAATCATAAATATTTGCCCCGACAAAAAAACGAACAGGTAACACACGt                     >  1:2124251/1‑150 (MQ=17)

                                                                                                                             ccaccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGAGAAGCGAGTCATACTGCACGACCCACCAATCTTAAAGATTGTCCCCGAGAAAAAAACTTACAGGTAACACACgg                     >  1:233826/1‑149 (MQ=18)

                                                                                                                                ccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTCAAAATCTGTCCGGTACCACCCggctg                  >  2:2021350/1‑146 (MQ=21)

                                                                                                                                ccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATg                  >  1:2682558/1‑150 (MQ=255)

                                                                                                                                ccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATg                  <  1:1227249/150‑1 (MQ=255)

                                                                                                                                ccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATg                  >  2:1722628/1‑150 (MQ=255)

                                                                                                                                ccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATg                  >  2:2513222/1‑150 (MQ=255)

                                                                                                                                 caGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATgg                 <  2:2654791/150‑1 (MQ=255)

                                                                                                                                 caGTTTTAACATCGAACTCAGATGTTATGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATgg                 <  2:2713248/150‑1 (MQ=255)

                                                                                                                                 caGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATgg                 <  1:563054/150‑1 (MQ=255)

                                                                                                                                 caGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATgg                 >  2:2942131/1‑150 (MQ=255)

                                                                                                                                  aGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCGCCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATggg                <  2:288757/150‑1 (MQ=255)

                                                                                                                                    ttttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTc              >  2:229983/1‑150 (MQ=255)

                                                                                                                                    ttttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAAACTTAAAGATTGACCCCGAGTAAAAAACAATCTGGTATCACGCGAAGGGGtg              >  1:366815/1‑149 (MQ=18)

                                                                                                                                    ttttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCAGCACGACCCACCCAACAAAAAGAGAGGCCCCGAGTAAAAAACTTACAGGTAACACACGAATGGTTc              >  1:577604/1‑150 (MQ=18)

                                                                                                                                    gtttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAATATCTTTCAGGTAACGCCCGTATGGGTc              >  1:683282/2‑150 (MQ=255)

                                                                                                                                           aTCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCt       <  1:2611439/150‑1 (MQ=255)

                                                                                                                                           aTCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTAGGGGTCGTTAGCt       >  2:2030879/1‑150 (MQ=255)

                                                                                                                                            tCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTc      >  2:1037606/1‑150 (MQ=255)

                                                                                                                                              aaaCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTAGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAg    >  1:423698/1‑150 (MQ=255)

                                                                                                                                                aCTCAGATGTTCAGGCTGCATGTTTACGGTGCTCCAGCAACGTCCAGTTGGGAGATCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGtt  <  1:1879216/150‑1 (MQ=37)

                                                                                                                                                aCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGtt  <  2:1009687/150‑1 (MQ=255)

                                                                                                                                                aCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGtt  >  1:1187017/1‑150 (MQ=255)

CACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTT  >  NC_000913/781092‑781385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`