Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 845,575 G→A 6.0% intergenic (‑95/+166) ybiO ← / ← glnQ mechanosensitive channel protein, intermediate conductance/glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913845,5750GA 6.0% 685.8 / 21.6 198intergenic (‑95/+166)ybiO/glnQmechanosensitive channel protein, intermediate conductance/glutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (96/90);  new base A (6/6);  total (102/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 203 total aligned reads displayed.

GCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCAT  >  NC_000913/845428‑845724
                                                                                                                                                   |                                                                                                                                                     
gcgTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCggg                                                                                                                                                     <  1:1851544/150‑1 (MQ=255)
gcgTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCggg                                                                                                                                                     >  2:1739438/1‑150 (MQ=255)
       cAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAgg                                                                                                                                              <  2:419898/150‑1 (MQ=255)
       cAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAgg                                                                                                                                              <  2:1013047/150‑1 (MQ=255)
       cAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAgg                                                                                                                                              <  2:345936/150‑1 (MQ=255)
         ggTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCACCCGAAAGgga                                                                                                                                            <  1:1974095/150‑1 (MQ=255)
          gTGCGCCCAGCAGGCATAAGAGGATGAACAGGATCCAACGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGggag                                                                                                                                           >  1:1960380/1‑150 (MQ=255)
          gTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGggag                                                                                                                                           >  1:431073/1‑150 (MQ=255)
           tGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCACCCGAAAGggagg                                                                                                                                          <  1:2842020/150‑1 (MQ=255)
              gcCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCGGTGAGAAATTAGAGAAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGggaggacg                                                                                                                                       >  1:1306349/1‑148 (MQ=255)
              gcCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAg                                                                                                                                       >  2:2215535/1‑150 (MQ=255)
              gcCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAg                                                                                                                                       >  2:1363075/1‑150 (MQ=255)
              gcCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAg                                                                                                                                       >  1:543731/1‑150 (MQ=255)
               cccAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAgg                                                                                                                                      <  2:229096/150‑1 (MQ=255)
               cccAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAgg                                                                                                                                      <  2:469612/150‑1 (MQ=255)
                        cagAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAg                                                                                                                             <  1:2376104/150‑1 (MQ=255)
                                gATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATTGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGc                                                                                                                     >  2:765020/1‑150 (MQ=255)
                                gATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGc                                                                                                                     >  1:2697529/1‑150 (MQ=255)
                                gATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGc                                                                                                                     <  1:1683042/150‑1 (MQ=255)
                                  tGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGATGGGTTATTAAGCAGGTTGCTg                                                                                                                   >  1:1303807/1‑150 (MQ=255)
                                  tGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTg                                                                                                                   <  2:1499005/150‑1 (MQ=255)
                                  tGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTg                                                                                                                   >  2:503451/1‑150 (MQ=255)
                                  tGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTg                                                                                                                   >  1:1588558/1‑150 (MQ=255)
                                  tGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGAGATTAAGCAGGTTGCTg                                                                                                                   >  1:1009520/1‑150 (MQ=255)
                                  ggAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCACAAAGATACGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGACAGGGAGGAAGGGATATTAAGCAGGTTGCTg                                                                                                                   >  2:2020273/2‑150 (MQ=255)
                                         gATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGt                                                                                                            >  1:1152990/1‑150 (MQ=255)
                                          aTCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTg                                                                                                           >  2:1445931/1‑150 (MQ=255)
                                           tCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGc                                                                                                          >  2:939973/1‑150 (MQ=255)
                                           tCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGc                                                                                                          >  2:2405244/1‑150 (MQ=255)
                                             cACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCgg                                                                                                        <  2:2149290/150‑1 (MQ=255)
                                             cACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCgg                                                                                                        >  2:540855/1‑150 (MQ=255)
                                             cACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCgg                                                                                                        <  2:1916368/150‑1 (MQ=255)
                                               ccGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGc                                                                                                      >  2:251803/1‑150 (MQ=255)
                                               ccGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGc                                                                                                      >  2:2771086/1‑150 (MQ=255)
                                               ccGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGc                                                                                                      >  2:2878936/1‑150 (MQ=255)
                                                 gCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCtt                                                                                                    >  2:1891057/1‑150 (MQ=255)
                                                 gCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCtt                                                                                                    >  2:2835278/1‑150 (MQ=255)
                                                   aTGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCtttt                                                                                                  >  2:394101/1‑150 (MQ=255)
                                                    tGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCttttt                                                                                                 <  1:1520541/150‑1 (MQ=255)
                                                    tGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCGGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCttttt                                                                                                 >  2:566326/1‑150 (MQ=255)
                                                     gACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCtttttt                                                                                                >  1:189858/1‑150 (MQ=255)
                                                     gACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCtttttt                                                                                                <  1:1181099/150‑1 (MQ=255)
                                                     gACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCtttttt                                                                                                <  1:1181156/150‑1 (MQ=255)
                                                     gACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCtttttt                                                                                                >  1:1641249/1‑150 (MQ=255)
                                                     gACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCtttttt                                                                                                <  2:1697669/150‑1 (MQ=255)
                                                      aCTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTg                                                                                               <  1:872432/150‑1 (MQ=255)
                                                        tcctccAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGcc                                                                                             <  2:2181883/150‑1 (MQ=255)
                                                         cctccAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCg                                                                                            <  1:1718930/150‑1 (MQ=255)
                                                               gTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATgcg                                                                                      <  1:2576639/150‑1 (MQ=255)
                                                                tGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATgcgg                                                                                     >  2:2156956/1‑150 (MQ=255)
                                                                 gagaAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATgcggc                                                                                    <  1:2180365/150‑1 (MQ=255)
                                                                     aaaTAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGa                                                                                <  2:2721861/150‑1 (MQ=255)
                                                                         agagCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGc                                                                            <  1:1331353/150‑1 (MQ=255)
                                                                            gCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCtt                                                                         <  2:836665/150‑1 (MQ=255)
                                                                               aaaaGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATc                                                                      <  1:1872743/150‑1 (MQ=255)
                                                                                    aTAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTcc                                                                 >  2:1137759/1‑150 (MQ=255)
                                                                                    aTAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTcc                                                                 <  1:646966/150‑1 (MQ=255)
                                                                                      aaGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTa                                                               >  1:629410/1‑150 (MQ=255)
                                                                                      aaGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGAAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTa                                                               >  1:1986659/1‑150 (MQ=255)
                                                                                        gTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACa                                                             >  1:2359380/1‑150 (MQ=255)
                                                                                            aGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGtt                                                         <  1:2904198/150‑1 (MQ=255)
                                                                                            aGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGtt                                                         >  2:1814962/1‑150 (MQ=255)
                                                                                              aTGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGAAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCa                                                       <  1:1417501/150‑1 (MQ=255)
                                                                                              aTGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGAAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCa                                                       <  2:2562352/150‑1 (MQ=255)
                                                                                               tGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCAt                                                      <  1:72523/150‑1 (MQ=255)
                                                                                               tGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCAt                                                      <  1:2118882/150‑1 (MQ=255)
                                                                                                gCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATg                                                     <  1:60141/150‑1 (MQ=255)
                                                                                                 cTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGc                                                    >  1:1775113/1‑150 (MQ=255)
                                                                                                 cTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGc                                                    >  1:1502696/1‑150 (MQ=255)
                                                                                                 cTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGc                                                    >  1:125067/1‑150 (MQ=255)
                                                                                                 cTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGGGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTACTACAAGTTCATGc                                                    >  1:376480/1‑150 (MQ=255)
                                                                                                    gaggagGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAAt                                                 >  2:2319492/1‑150 (MQ=255)
                                                                                                          gggCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTtaat                                           >  2:1395718/1‑150 (MQ=255)
                                                                                                            gCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTtaataa                                         >  1:228595/1‑150 (MQ=255)
                                                                                                               aTTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGtt                                      >  1:2061613/1‑150 (MQ=255)
                                                                                                                ttttAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTg                                     <  2:1083440/150‑1 (MQ=255)
                                                                                                                  ttAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGcc                                   <  2:2903168/150‑1 (MQ=255)
                                                                                                                     aaTGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGa                                >  1:2172146/1‑150 (MQ=255)
                                                                                                                       tGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGata                              <  2:1641249/150‑1 (MQ=255)
                                                                                                                       tGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGata                              <  2:189858/150‑1 (MQ=255)
                                                                                                                          gagGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATcc                           >  1:2333789/1‑150 (MQ=255)
                                                                                                                          gagGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATcc                           >  1:577672/1‑150 (MQ=255)
                                                                                                                          gagGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATcc                           <  1:2810004/150‑1 (MQ=255)
                                                                                                                           agGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCt                          <  1:332941/150‑1 (MQ=255)
                                                                                                                           agGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCt                          <  2:2448658/150‑1 (MQ=255)
                                                                                                                             gAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGt                        >  2:1267191/1‑150 (MQ=255)
                                                                                                                                     tgtgCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACACGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGa                <  2:1536700/150‑1 (MQ=255)
                                                                                                                                       ttccTCCCTTTCACCCGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATa              >  1:230297/3‑150 (MQ=255)
                                                                                                                                         ccTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAg            >  1:1057217/1‑150 (MQ=255)
                                                                                                                                          cTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGc           <  2:1135166/150‑1 (MQ=255)
                                                                                                                                          cTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGc           <  1:996946/150‑1 (MQ=255)
                                                                                                                                          cTCCCTTTCACCCGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGc           >  2:1244229/1‑150 (MQ=255)
                                                                                                                                           tCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCg          >  2:2403017/1‑150 (MQ=255)
                                                                                                                                           tCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCg          >  2:1808996/1‑150 (MQ=255)
                                                                                                                                            cccTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGt         >  1:71584/1‑150 (MQ=255)
                                                                                                                                              cTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAg       <  2:1152990/150‑1 (MQ=255)
                                                                                                                                              cTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAg       >  1:2502474/1‑150 (MQ=255)
                                                                                                                                              cTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAg       >  1:1976768/1‑150 (MQ=255)
                                                                                                                                               tttCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAgc      <  2:2637454/150‑1 (MQ=255)
                                                                                                                                                   gggTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCAt  >  2:1603280/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCAT  >  NC_000913/845428‑845724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: