BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,286,965	A→G	10.9%	intergenic (+439/+101)	narI → / ← rttR	nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,286,965	0	A	G	10.9%	251.3 / 11.0	101	intergenic (+439/+101)	narI/rttR	nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
Reads supporting (aligned to +/- strand): ref base A (34/49); new base G (7/2); total (46/55)
Fisher's exact test for biased strand distribution p-value = 7.26e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

TCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGG  >  NC_000913/1286817‑1287113

tcATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGACTGCGGCAACCCTCTCTCGCTGAc                                                                                                                                                     >  1:2567047/1‑150 (MQ=255)

 cagccgtCCCCGCTTGGGCAGAAGATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACg                                                                                                                                                    <  2:1665246/144‑1 (MQ=255)

   tCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCt                                                                                                                                                  >  2:2279698/1‑150 (MQ=255)

   tCCTTCCCCGCTTGGGCAGAATATTTGATTGAGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCt                                                                                                                                                  >  2:2133050/1‑150 (MQ=255)

      ttCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAGAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCgg                                                                                                                                               >  2:558603/1‑149 (MQ=255)

      ttCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGACAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                               >  2:2273984/1‑150 (MQ=255)

      ttCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGa                                                                                                                                               >  2:558609/1‑150 (MQ=255)

      ttCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGa                                                                                                                                               >  2:2667387/1‑150 (MQ=255)

      ttCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGGGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCgc                                                                                                                                               >  2:2866662/1‑149 (MQ=255)

       tgcgcGCTTGGGCTGAATATTTGATGGCGGATTCGTTTGCGCTTTCCGGGGCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAg                                                                                                                                              <  2:2373823/146‑1 (MQ=14)

       tCCGCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGCAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAg                                                                                                                                              <  2:2373248/150‑1 (MQ=255)

       tCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAg                                                                                                                                              >  2:1169839/1‑150 (MQ=255)

       tCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTCTGAGAATTCCGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAg                                                                                                                                              <  2:2281002/150‑1 (MQ=255)

        ccccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGc                                                                                                                                             <  1:2838253/150‑1 (MQ=255)

         cccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGcc                                                                                                                                            >  1:1553227/1‑150 (MQ=255)

           cGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGa                                                                                                                                          <  2:2863245/150‑1 (MQ=255)

            gCTTGGGCAGAATTTTTGATTGCGGATTCGTTTGAGAATTCCGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGaa                                                                                                                                         <  1:2249643/150‑1 (MQ=255)

            gCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGaa                                                                                                                                         <  1:2249639/150‑1 (MQ=255)

            gCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGaa                                                                                                                                         <  2:2280248/150‑1 (MQ=255)

             cTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAAc                                                                                                                                        <  2:2405697/150‑1 (MQ=255)

             cTTGGGCAGAATATTTGATTGCGGAGTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAAc                                                                                                                                        <  2:2743678/150‑1 (MQ=255)

               tGGGCAGAATATTTGATTGCGGATTCGTTTGAGATTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCt                                                                                                                                      <  1:35553/150‑1 (MQ=255)

               tGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCt                                                                                                                                      <  1:174160/150‑1 (MQ=255)

                 ggCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCttg                                                                                                                                    <  1:1169839/150‑2 (MQ=18)

                   cAGATTATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGt                                                                                                                                  <  2:2262233/150‑1 (MQ=255)

                   cAGAAGCTTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGt                                                                                                                                  <  2:2262178/150‑1 (MQ=255)

                       atatTTTATTTCGGATTAGTTTGAGAATTCCGTGTCTTTAGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGaa                                                                                                                              <  1:605500/150‑1 (MQ=14)

                       atatTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGaa                                                                                                                              <  1:1975839/150‑1 (MQ=255)

                       atatTTGATTGCGGATTCGTTTGAGAATTCCCGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGaa                                                                                                                              <  1:1005812/150‑1 (MQ=255)

                         atTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGc                                                                                                                            <  1:275701/150‑1 (MQ=255)

                           ttGATTGCGGATTCGTTTGAGAATTCCGGGTATTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCtt                                                                                                                          <  2:1806365/150‑1 (MQ=255)

                           ttGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCACTCCCAGTCGCTCCAGCCGAACCTTGGTCGAAGCtt                                                                                                                          >  2:515988/1‑150 (MQ=14)

                           ttGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCACACGCAGGCGCTCGAGCCGAAACTTGGTCGAAGCtt                                                                                                                          >  2:276176/1‑150 (MQ=11)

                                  cGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATcc                                                                                                                   <  2:1843187/150‑1 (MQ=255)

                                     aTTCGTTTGTGTTTTGCGGGTTTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTc                                                                                                                <  2:912256/150‑1 (MQ=14)

                                              agaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGCACTCACGCTGACGCTCGAGCCCAACCATAGTCGAAGCTTCTAATCCATACCCCCAAgg                                                                                                       >  1:397474/1‑150 (MQ=14)

                                              agaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGACTGCGGCAACGCACTCTCGCAGACGCTCGAGCAGAACCGTAGTCGAAGCTTCACAAACATACCCCCATgg                                                                                                       >  1:1703743/1‑150 (MQ=14)

                                               gaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCATCGCCCATCGGGTCGTTGCCTGCCGCAACGCACTCTCGCTGACCCAACATCCGAAACGTGCTCAAAGAATCTCATCCATACCCGCATgtg                                                                                                      >  2:2595192/1‑148 (MQ=14)

                                               gaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGAGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTCCCGCAAAGCGCTCTCGCTGACGCGAGAGCCGAACCATAGTCGAAGCATATCATCCATACCCGCATggg                                                                                                      >  2:1310100/1‑150 (MQ=14)

                                                   tgctggtcTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGa                                                                                                  <  2:1200797/143‑1 (MQ=255)

                                                   tcggggtCTTCTGCAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCACGGGCAGa                                                                                                  <  2:2656635/147‑1 (MQ=11)

                                                   tCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGa                                                                                                  <  2:471169/150‑1 (MQ=255)

                                                   tCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGa                                                                                                  <  2:1599406/150‑1 (MQ=255)

                                                   tCCGGGGATTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGa                                                                                                  <  2:956260/150‑1 (MQ=255)

                                                      ggttattttGCAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAata                                                                                               <  1:2649391/145‑1 (MQ=255)

                                                      ggggCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAata                                                                                               <  1:2649162/150‑1 (MQ=39)

                                                      ggggCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCCCATGGGCAGAata                                                                                               >  2:2604261/1‑150 (MQ=255)

                                                        ggCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGGCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGGCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGTATAtt                                                                                             >  2:243401/1‑150 (MQ=11)

                                                         tattttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAttt                                                                                            <  2:2402856/148‑1 (MQ=37)

                                                         gCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCATAGTCGAAGCTTCTCATCCATCCCCCCATGGGCAGAATAttt                                                                                            >  2:2026967/1‑150 (MQ=255)

                                                          cTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTg                                                                                           <  2:1341115/150‑1 (MQ=38)

                                                                   gcgATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGAt                                                                                  >  2:1518025/3‑150 (MQ=39)

                                                                       tggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGc                                                                              <  2:2138301/150‑1 (MQ=40)

                                                                         gtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCtt                                                                            <  2:826263/150‑1 (MQ=255)

                                                                              gggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGGTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGagag                                                                       <  1:1506372/150‑1 (MQ=255)

                                                                                 ggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCGTAGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCAGAATATTTGATTGCGGATTCGGTTGGGAGTTc                                                                    >  2:2064826/1‑150 (MQ=14)

                                                                                     ggATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAggg                                                                >  2:2123539/1‑150 (MQ=255)

                                                                                      gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCTGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                               >  1:2371042/1‑150 (MQ=255)

                                                                                        ttACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGTAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGTGAGTTCAGGGTCt                                                             >  2:1845508/1‑150 (MQ=255)

                                                                                            tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGGGCCGAACCGTCGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCAGGATCTTTGATTTCGGTGTCGCGTGGGAGTTCCGGGACTGTTg                                                         >  2:2521353/1‑150 (MQ=14)

                                                                                            tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCTCGCTGACGCGCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTg                                                         >  2:2521339/1‑150 (MQ=255)

                                                                                              aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                                       <  2:2750182/150‑1 (MQ=255)

                                                                                               gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                                      <  1:2270181/150‑1 (MQ=255)

                                                                                               gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                                      <  1:2509617/150‑1 (MQ=255)

                                                                                               gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                                      <  1:465852/150‑1 (MQ=255)

                                                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGaa                                                                                                 >  2:1956674/1‑106 (MQ=40)

                                                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                                                     <  2:846365/150‑1 (MQ=255)

                                                                                                 gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt                                                    <  1:2358645/150‑1 (MQ=255)

                                                                                                  cTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGGCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGGTTGGTAGTTCAGGGACTTTTGAAAGTg                                                   >  2:745931/1‑150 (MQ=11)

                                                                                                   tGCGCGCTTCGCCCTTCTGTTAGTTGCCTGCGGCAAAGCTCTCTCGCTGTCTCTCGATCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGTCAGAATATTTGATTGCGGATTCTATTGTGAGTTCAGGGACTTTTGAAAGTGa                                                  <  1:2692455/150‑1 (MQ=14)

                                                                                                   tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGATTGAGAGTTCAGGGTCTTTTGAAAGTGa                                                  <  1:73917/150‑1 (MQ=255)

                                                                                                   tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGa                                                  <  1:2692412/150‑1 (MQ=255)

                                                                                                          ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCAGTATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggt                                           >  2:374339/1‑150 (MQ=255)

                                                                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCCGAATATTTGATTGCGGATTCGCTTGTGAGTTCAGGGACTTTTGAAAGTGAGGGTGGTggg                                        >  2:1897996/1‑150 (MQ=255)

                                                                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggg                                        <  2:2157950/150‑1 (MQ=255)

                                                                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggg                                        <  1:1518025/150‑1 (MQ=255)

                                                                                                             gCCCGTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggg                                        <  1:2749665/150‑1 (MQ=255)

                                                                                                              cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTgggg                                       >  2:1629335/1‑150 (MQ=255)

                                                                                                              cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGCTTGAGAGTTCAGGGACTTGTGTAAGGGATGGTGTTgggg                                       >  2:1629328/1‑150 (MQ=14)

                                                                                                                  tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAg                                   <  2:514746/150‑1 (MQ=255)

                                                                                                                    gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                 <  1:2604261/150‑1 (MQ=255)

                                                                                                                      agcGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                               >  2:2268185/3‑150 (MQ=255)

                                                                                                                          ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCTTCTCATCCTTCCCCGCCGGGGCAGAAAATTTGATTGCGGAGTCGCGTGGGAGGTCAGGGACTTTTGAAAGTGATGGTGGGGGGGGAAGGagaacac                           >  1:2027488/1‑144 (MQ=14)

                                                                                                                          ttGCCTGCGGCAACGCTCTATCGCTGTCGCTCGAGCCGAACCTTAGTGGAATGTTATAATACTTACCCGCATGGGCCGAATATTTTTTTTGGGTTTGGGTTGTGTGTTAAGGGACTTTTTTTATTGATTGTTGTGGGGGTAGGTTTTCta                           >  1:310801/1‑149 (MQ=9)

                                                                                                                           tGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCa                          <  1:2259477/150‑1 (MQ=255)

                                                                                                                           tGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGATGGGGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAcaaa                          >  2:1795581/1‑147 (MQ=255)

                                                                                                                             ccgcaggCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAgc                        >  1:2652720/6‑150 (MQ=255)

                                                                                                                                  ggCAACGCTCTCTCGCTGACGCTCGAGCCGACCCTTAGTCGAAGCTTCTCATCCTTCCCCGCGTGGGCAGAATATTTGAGTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGCTGGTGGGGGGGGAAGGATTACTCCGCGCGgc                   >  2:1341315/1‑150 (MQ=17)

                                                                                                                                  ggCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGTGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAGTCAGCGCTgc                   >  2:36411/1‑150 (MQ=255)

                                                                                                                                  ggCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgc                   >  2:1531117/1‑150 (MQ=255)

                                                                                                                                    cAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACACAGAGCTgcgc                 >  2:2403554/1‑150 (MQ=255)

                                                                                                                                       cGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCt              >  2:413759/1‑150 (MQ=255)

                                                                                                                                       cGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCt              >  1:2900486/1‑150 (MQ=255)

                                                                                                                                       cGCTCTCTCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCCGACGATTTGATTGCGGATTCGCGGGCGAGTTCAGGGACTTGTGAAAGTGATGGGGGGGGGGGAAGGCTTACTCACCGCGGCGCGCt              >  1:1601666/1‑150 (MQ=17)

                                                                                                                                        gCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCtt             <  2:2371042/150‑1 (MQ=255)

                                                                                                                                        gCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCtt             <  1:2578647/150‑1 (MQ=255)

                                                                                                                                              tcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCATCGCCCt       >  1:679341/1‑150 (MQ=255)

                                                                                                                                                gCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTc     <  2:533027/150‑1 (MQ=255)

                                                                                                                                                  tGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCgg   <  1:2935084/150‑1 (MQ=255)

                                                                                                                                                   gACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCggg  >  2:157438/1‑150 (MQ=255)

TCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGG  >  NC_000913/1286817‑1287113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`