Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,568,915 G→A 5.8% intergenic (‑92/+39) yddW ← / ← gadC liprotein, glycosyl hydrolase homolog/glutamate:gamma‑aminobutyric acid antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,568,9150GA 5.8% 478.7 / 12.8 138intergenic (‑92/+39)yddW/gadCliprotein, glycosyl hydrolase homolog/glutamate:gamma‑aminobutyric acid antiporter
Reads supporting (aligned to +/- strand):  ref base G (69/61);  new base A (2/6);  total (71/67)
Fisher's exact test for biased strand distribution p-value = 1.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 138 total aligned reads displayed.

AACTAGTATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGC  >  NC_000913/1568767‑1569063
                                                                                                                                                    |                                                                                                                                                    
aaCTAGTATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGa                                                                                                                                                     <  2:1307499/150‑1 (MQ=255)
aaCTAGTATCGCTGGTCTTCTAATCGTTAATTTATTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGa                                                                                                                                                     >  2:2532422/1‑150 (MQ=255)
   tAGTATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGaagt                                                                                                                                                  >  2:2438851/1‑148 (MQ=255)
     gTATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTAt                                                                                                                                                >  1:539547/1‑150 (MQ=255)
      tATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATc                                                                                                                                               >  2:1811169/1‑150 (MQ=255)
      tATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATc                                                                                                                                               >  2:795144/1‑150 (MQ=255)
      tATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATc                                                                                                                                               >  2:1620283/1‑150 (MQ=255)
      tATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATc                                                                                                                                               >  2:708226/1‑150 (MQ=255)
      tATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATc                                                                                                                                               >  2:708216/1‑150 (MQ=255)
      tATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATc                                                                                                                                               >  1:1683923/1‑150 (MQ=255)
      tATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATAGGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGCTGAATTATc                                                                                                                                               >  2:2830091/1‑150 (MQ=255)
        tCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATAAGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGc                                                                                                                                             >  1:515267/1‑150 (MQ=255)
         cGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCt                                                                                                                                            <  1:597445/150‑1 (MQ=255)
         cGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATAATTCATCGCt                                                                                                                                            <  2:528220/150‑1 (MQ=255)
         cGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATAATTCATCGCt                                                                                                                                            <  2:2013648/150‑1 (MQ=255)
         cGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATAATTCATCGCt                                                                                                                                            <  2:2013637/150‑1 (MQ=255)
          gCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTc                                                                                                                                           >  1:2903687/1‑150 (MQ=255)
          gCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATAATTCATCGCTc                                                                                                                                           <  1:1319486/150‑1 (MQ=255)
           cTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATAATTCATCGCTcc                                                                                                                                          <  2:2538597/150‑1 (MQ=255)
                cttctAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATAATTCATCGCTCCCtttt                                                                                                                                     <  1:412637/150‑3 (MQ=255)
                 ttctAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATAAGAAAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTc                                                                                                                                    >  2:1901134/1‑150 (MQ=255)
                  tctAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGCCt                                                                                                                                   >  1:2029910/1‑150 (MQ=255)
                   ctAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCtt                                                                                                                                  >  1:1144563/1‑150 (MQ=255)
                      aTCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTtata                                                                                                                               >  1:1580608/1‑150 (MQ=255)
                       tCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATaa                                                                                                                              <  2:2694554/150‑1 (MQ=255)
                               ttCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAg                                                                                                                      <  1:2321843/150‑1 (MQ=255)
                                  ttGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACa                                                                                                                   >  2:2062098/1‑150 (MQ=255)
                                    gTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATg                                                                                                                 >  2:1160414/1‑150 (MQ=255)
                                          ggAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAgt                                                                                                           >  1:1889993/1‑150 (MQ=255)
                                          ggAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAgt                                                                                                           >  1:1220203/1‑150 (MQ=255)
                                           gAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCGATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAgtg                                                                                                          >  1:2298259/1‑150 (MQ=255)
                                           gAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAgtg                                                                                                          >  1:5925/1‑150 (MQ=255)
                                           gAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAgtg                                                                                                          >  1:1282597/1‑150 (MQ=255)
                                           gAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAgtg                                                                                                          >  1:2756257/1‑150 (MQ=255)
                                              cAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGttt                                                                                                       <  1:753555/150‑1 (MQ=255)
                                               aGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTc                                                                                                      <  1:415362/150‑1 (MQ=255)
                                               aGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTc                                                                                                      <  1:504057/150‑1 (MQ=255)
                                                 atatCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCtt                                                                                                    <  1:188541/150‑1 (MQ=255)
                                                   atCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGt                                                                                                  <  2:349809/150‑1 (MQ=255)
                                                      cATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGtcat                                                                                               >  2:1693945/1‑150 (MQ=255)
                                                       atatGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGtcatt                                                                                              <  2:444770/150‑1 (MQ=255)
                                                            tgCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATtcatca                                                                                         >  1:2502245/1‑150 (MQ=255)
                                                               tGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACaa                                                                                      >  1:992022/1‑150 (MQ=255)
                                                               tGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACaa                                                                                      >  1:1813834/1‑150 (MQ=255)
                                                                  tttCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAatat                                                                                   >  1:2733438/1‑150 (MQ=255)
                                                                   ttCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAAtata                                                                                  <  1:1929390/150‑1 (MQ=255)
                                                                    tCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAg                                                                                 <  2:1543689/150‑1 (MQ=255)
                                                                     ccGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgt                                                                                <  1:333333/150‑1 (MQ=255)
                                                                     ccGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgt                                                                                <  2:2212783/150‑1 (MQ=255)
                                                                     ccGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgt                                                                                <  2:2717382/150‑1 (MQ=255)
                                                                      cGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgtg                                                                               <  1:475427/150‑1 (MQ=255)
                                                                      cGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgtg                                                                               <  1:475448/150‑1 (MQ=255)
                                                                      cGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgtg                                                                               >  1:1321546/1‑150 (MQ=255)
                                                                       ggTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgtgt                                                                              >  1:2657429/1‑150 (MQ=255)
                                                                       ggTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgtgt                                                                              >  1:594272/1‑150 (MQ=255)
                                                                        gTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGtgtg                                                                             <  2:984745/150‑1 (MQ=255)
                                                                         tAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtgg                                                                            >  2:1269692/1‑150 (MQ=255)
                                                                               gATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACg                                                                      <  1:806424/150‑1 (MQ=255)
                                                                                aTGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGt                                                                     <  2:477555/150‑1 (MQ=255)
                                                                                   tgCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCa                                                                  >  1:1638714/1‑150 (MQ=255)
                                                                                   tgCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCa                                                                  >  1:1109887/1‑150 (MQ=255)
                                                                                     cgcTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAAGAGAGTGTGGGGAACGTGCAcg                                                                >  2:27925/1‑150 (MQ=255)
                                                                                          aTTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGt                                                           >  1:380459/1‑150 (MQ=255)
                                                                                             ctcGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCa                                                        <  2:895283/150‑1 (MQ=255)
                                                                                              tcGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAg                                                       >  1:2844948/1‑150 (MQ=255)
                                                                                                gTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGga                                                     <  2:805370/150‑1 (MQ=255)
                                                                                                   cTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaaga                                                  <  1:2495113/150‑1 (MQ=255)
                                                                                                   cTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaaga                                                  <  1:2438851/150‑1 (MQ=255)
                                                                                                    ttATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaagaa                                                 <  1:2675774/150‑1 (MQ=255)
                                                                                                     tATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGaagaag                                                <  2:2694760/150‑1 (MQ=255)
                                                                                                          ccGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGAcc                                           >  1:2082373/1‑150 (MQ=255)
                                                                                                            gTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCtt                                         >  2:891585/1‑150 (MQ=255)
                                                                                                            gTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCtt                                         >  1:330009/1‑150 (MQ=255)
                                                                                                               aaGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTg                                      <  1:270222/150‑1 (MQ=255)
                                                                                                               aaGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTg                                      <  1:25445/150‑1 (MQ=255)
                                                                                                               aaGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTg                                      >  1:2160952/1‑150 (MQ=255)
                                                                                                                 gTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGt                                    <  1:1400144/150‑1 (MQ=255)
                                                                                                                 gTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGt                                    <  2:921626/150‑1 (MQ=255)
                                                                                                                 gTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGt                                    <  1:1365052/150‑1 (MQ=255)
                                                                                                                 gTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGt                                    >  2:1608338/1‑150 (MQ=255)
                                                                                                                  tCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag                                                   >  2:1325336/1‑134 (MQ=255)
                                                                                                                  tCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTg                                   >  1:2015951/1‑150 (MQ=255)
                                                                                                                  tCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTg                                   >  1:1360457/1‑150 (MQ=255)
                                                                                                                    aaTACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCg                                 >  2:2835352/1‑150 (MQ=255)
                                                                                                                    aaTACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCg                                 >  1:1266039/1‑150 (MQ=255)
                                                                                                                     aTACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGt                                <  1:2271651/150‑1 (MQ=255)
                                                                                                                     aTACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGt                                <  1:1215142/150‑1 (MQ=255)
                                                                                                                       aCGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTc                              <  1:2671147/150‑1 (MQ=255)
                                                                                                                               aagcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGtt                      <  1:1901134/150‑1 (MQ=255)
                                                                                                                               aagcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGtt                      <  2:1144563/150‑1 (MQ=255)
                                                                                                                                 gcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGa                    <  1:2471328/150‑1 (MQ=255)
                                                                                                                                    cGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTg                 >  2:765870/1‑150 (MQ=255)
                                                                                                                                    cGAAAAAGGGAGCGATGAATTATCGCACCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCAAAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTg                 >  2:1333204/1‑150 (MQ=255)
                                                                                                                                     gAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTgg                <  1:2520900/150‑1 (MQ=255)
                                                                                                                                     gAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTgg                >  2:1176639/1‑150 (MQ=255)
                                                                                                                                     gAAAAAGGGAGCGATGAATCATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTgg                <  2:49714/150‑1 (MQ=255)
                                                                                                                                      gacaaGGGAGCGATAATTCATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGc               >  1:239086/4‑150 (MQ=255)
                                                                                                                                         aaGGGAGCGATAATTCATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTcc            >  2:835928/1‑150 (MQ=255)
                                                                                                                                                 gATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGAc    >  1:29367/1‑150 (MQ=255)
                                                                                                                                                   tGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGc  >  1:1893367/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AACTAGTATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGC  >  NC_000913/1568767‑1569063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: