Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,752,027 C→T 7.9% A602T (GCA→ACA)  ydhV ← putative oxidoreductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,752,0270CT 7.9% 583.6 / 28.5 176A602T (GCA→ACA) ydhVputative oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base C (81/81);  new base T (7/7);  total (88/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 184 total aligned reads displayed.

ATCCATTTTGTCAGTACCTTCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTAT  >  NC_000913/1751878‑1752176
                                                                                                                                                     |                                                                                                                                                     
aTCCATTTTGTCAGTACCTTCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGc                                                                                                                                                       >  1:117277/1‑150 (MQ=255)
aTCCATTTTGTCAGTACCTTCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGc                                                                                                                                                       >  2:773546/1‑150 (MQ=255)
aTCCATTTTGTCAGTACCTTCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATAAGCTCTGc                                                                                                                                                       >  1:816159/1‑150 (MQ=255)
  ccATTTTGTCAGTACCTTCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAg                                                                                                                                                     <  1:131484/150‑1 (MQ=255)
     ttttGTCAGTACCTTCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTa                                                                                                                                                  >  2:28455/1‑150 (MQ=255)
     ttttGTCAGTACCTTCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTa                                                                                                                                                  >  2:28376/1‑150 (MQ=255)
      tttGTCAGTACCTTCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTaa                                                                                                                                                 <  2:1952311/150‑1 (MQ=255)
          tCAGTACCTTCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCt                                                                                                                                             >  2:1798109/1‑150 (MQ=255)
          tCAGTACCTTCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCt                                                                                                                                             >  2:2794899/1‑150 (MQ=255)
            aGTACCTTCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTaa                                                                                                                                           <  2:1455640/150‑1 (MQ=255)
            aGTACCTTCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTaa                                                                                                                                           <  2:2139654/150‑1 (MQ=255)
            aGTACCTTCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTaa                                                                                                                                           <  2:667087/150‑1 (MQ=255)
             gTACCTTCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAAt                                                                                                                                          >  1:2728456/1‑150 (MQ=255)
               aCCTTCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAAttt                                                                                                                                        <  2:2105119/150‑1 (MQ=255)
                  ttCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTc                                                                                                                                     >  2:2925285/1‑150 (MQ=255)
                      gTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGa                                                                                                                                 <  2:1971583/150‑1 (MQ=255)
                      gTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGa                                                                                                                                 <  1:2038438/150‑1 (MQ=255)
                      gTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGa                                                                                                                                 <  1:2038398/150‑1 (MQ=255)
                       tAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAg                                                                                                                                >  2:2119611/1‑150 (MQ=255)
                         aaGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTACATCTAATTTTTCCTGAGTc                                                                                                                              >  1:1568328/1‑150 (MQ=255)
                         aaGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTc                                                                                                                              <  2:1113699/150‑1 (MQ=255)
                          aGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCa                                                                                                                             >  2:2502805/1‑150 (MQ=255)
                             ccGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCAtct                                                                                                                          <  1:377504/150‑1 (MQ=255)
                              cGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCAtctc                                                                                                                         >  2:374172/1‑150 (MQ=255)
                                   tCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGc                                                                                                                    <  1:1629504/150‑1 (MQ=255)
                                        ggttCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTg                                                                                                               >  1:2667506/4‑150 (MQ=255)
                                         gATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGa                                                                                                              >  2:1242636/1‑150 (MQ=255)
                                          aTCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGAt                                                                                                             >  1:2582405/1‑150 (MQ=255)
                                          aTCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGAt                                                                                                             >  2:2237837/1‑150 (MQ=255)
                                          aTCCTAGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCGAAATCTAATTTTTCCTGAGTCATCTCTTAGCAGGTGAt                                                                                                             >  1:2040620/1‑150 (MQ=255)
                                            ccTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCg                                                                                                           <  2:1457413/150‑1 (MQ=255)
                                              ttGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTCAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCt                                                                                                         >  1:904826/1‑150 (MQ=255)
                                              ttGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCt                                                                                                         >  1:800730/1‑150 (MQ=255)
                                               tGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCtt                                                                                                        <  2:1523263/150‑1 (MQ=255)
                                                  cGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTga                                                                                                     <  2:458119/150‑1 (MQ=255)
                                                  cGAATACCCAGGAACAGATAAGATCGTGTACATTACGCATATCATTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTga                                                                                                     <  2:444278/150‑1 (MQ=255)
                                                         ccAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAAttt                                                                                              >  2:1511214/1‑150 (MQ=255)
                                                         ccAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAAttt                                                                                              >  1:973365/1‑150 (MQ=255)
                                                         ccAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAAttt                                                                                              >  2:1416807/1‑150 (MQ=255)
                                                         ccAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAAttt                                                                                              >  2:2590793/1‑150 (MQ=255)
                                                          cAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTg                                                                                             <  2:2459736/150‑1 (MQ=255)
                                                          cAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTg                                                                                             >  2:2901353/1‑150 (MQ=255)
                                                             gAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTGTGAAGAATTTGGCt                                                                                          >  2:2688001/1‑150 (MQ=255)
                                                              aaCAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGTAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCtt                                                                                         >  2:1966237/1‑150 (MQ=255)
                                                              aaCAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGTAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCtt                                                                                         >  2:1606883/1‑150 (MQ=255)
                                                              aaCAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGTAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCtt                                                                                         >  2:1344608/1‑150 (MQ=255)
                                                                  gATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTAAAg                                                                                     <  1:28376/150‑1 (MQ=255)
                                                                     aaGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAgcgc                                                                                  <  2:1381983/150‑1 (MQ=255)
                                                                         tCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGa                                                                              >  2:463294/1‑150 (MQ=255)
                                                                         tCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGa                                                                              >  2:723999/1‑150 (MQ=255)
                                                                           gtgtTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATc                                                                            <  1:1771250/150‑1 (MQ=255)
                                                                           gtgtTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATc                                                                            >  1:1838695/1‑150 (MQ=255)
                                                                           gtgtTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATc                                                                            <  1:173440/150‑1 (MQ=255)
                                                                           gtgtTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATc                                                                            <  1:1939112/150‑1 (MQ=255)
                                                                                  ttACGCATATCTTTGGTTTGCTTCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCgg                                                                     <  2:1755549/150‑1 (MQ=255)
                                                                                  ttACGCATATCTATGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTACAAGCGCCAGATCGCCCCgg                                                                     >  1:2164164/1‑150 (MQ=255)
                                                                                    aCGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTa                                                                   >  1:191368/1‑150 (MQ=255)
                                                                                      gCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAAt                                                                 >  2:333344/1‑150 (MQ=255)
                                                                                      gCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATAGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAAt                                                                 >  2:333354/1‑150 (MQ=255)
                                                                                        atatCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTa                                                               >  1:2373965/1‑150 (MQ=255)
                                                                                         tatCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTATTTAc                                                              <  2:748874/150‑1 (MQ=255)
                                                                                         tatCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTAc                                                              <  1:2704966/150‑1 (MQ=255)
                                                                                           tCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACgg                                                            <  1:1065810/150‑1 (MQ=255)
                                                                                           tCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACgg                                                            >  2:1929250/1‑150 (MQ=255)
                                                                                             tttGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCt                                                          >  1:2515582/1‑150 (MQ=255)
                                                                                             tttGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCt                                                          >  1:1242051/1‑150 (MQ=255)
                                                                                              ttGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCtt                                                         <  2:822442/150‑1 (MQ=255)
                                                                                              ttGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCtt                                                         <  1:1085670/150‑1 (MQ=255)
                                                                                                ggTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCtttt                                                       <  1:2304997/150‑1 (MQ=255)
                                                                                                  tttGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCa                                                     <  2:1838695/150‑1 (MQ=255)
                                                                                                    tGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAgc                                                   <  2:2855647/150‑1 (MQ=255)
                                                                                                        tCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCAGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAgcggcg                                               >  1:2358919/1‑150 (MQ=255)
                                                                                                         cAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGa                                              <  2:2065282/150‑1 (MQ=255)
                                                                                                          aGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGaa                                             >  1:485839/1‑150 (MQ=255)
                                                                                                           gTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGaaa                                            <  2:837860/150‑1 (MQ=255)
                                                                                                           gTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGaaa                                            <  2:2305848/150‑1 (MQ=255)
                                                                                                            ttttACCGTGTAGGCACGATGCAACGTACAAATACGCTCTGCAGCTCAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGACATTGGCTTCAAGCGCCAGATCGCCCCGTTAATTACGGCTTTGCAGCGGCGAAAc                                           >  2:2527967/1‑150 (MQ=255)
                                                                                                            ttttACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAAc                                           <  1:2925285/150‑1 (MQ=255)
                                                                                                              ttACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACgg                                         >  1:517609/1‑150 (MQ=255)
                                                                                                               tACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGt                                        <  1:2643038/150‑1 (MQ=255)
                                                                                                               tACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGt                                        <  1:1509061/150‑1 (MQ=255)
                                                                                                                 ccGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCa                                      <  2:2749137/150‑1 (MQ=255)
                                                                                                                     ggaGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTgg                                  <  1:2729658/148‑1 (MQ=255)
                                                                                                                      tAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGc                                 >  1:1680003/1‑150 (MQ=255)
                                                                                                                          cACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGa                             <  1:571046/150‑1 (MQ=255)
                                                                                                                              aTGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCa                         <  2:604536/150‑1 (MQ=255)
                                                                                                                                   aCGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGc                    <  1:2174726/150‑1 (MQ=255)
                                                                                                                                   aCGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGc                    >  1:1818668/1‑150 (MQ=255)
                                                                                                                                    cGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGca                   >  1:119290/1‑150 (MQ=255)
                                                                                                                                     gTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGcac                  >  2:2624378/1‑150 (MQ=255)
                                                                                                                                      tAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGcaca                 >  1:135377/1‑150 (MQ=255)
                                                                                                                                       aaaaaTACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATAGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAg                <  1:333354/150‑1 (MQ=255)
                                                                                                                                        aaaaTACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAgg               >  1:2868579/1‑150 (MQ=255)
                                                                                                                                        aaaaTACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAgg               <  1:2304959/150‑1 (MQ=255)
                                                                                                                                         aaaTACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAggg              >  1:505424/1‑150 (MQ=255)
                                                                                                                                            tACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGa           <  1:919505/150‑1 (MQ=255)
                                                                                                                                              cGCTCTGTAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACg         <  1:1966237/150‑1 (MQ=255)
                                                                                                                                              cGCTCTGTAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACg         <  1:1345754/150‑1 (MQ=255)
                                                                                                                                              cGCTCTGTAGATAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACg         <  1:1606883/150‑1 (MQ=255)
                                                                                                                                                     cAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTAt  >  2:1303225/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCCATTTTGTCAGTACCTTCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTAT  >  NC_000913/1751878‑1752176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: