BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,818,049	T→A	5.2%	intergenic (‑189/+10)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,049	0	T	A	5.2%	508.1 / 10.9	153	intergenic (‑189/+10)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (84/60); new base A (4/5); total (88/65)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

CTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGAT  >  NC_000913/2817901‑2818198

ctaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTc                                                                                                                                                      >  2:1553178/1‑150 (MQ=255)

 taAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCg                                                                                                                                                     <  2:1197202/150‑1 (MQ=255)

 taAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCg                                                                                                                                                     <  2:1197099/150‑1 (MQ=255)

 taAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATAATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCg                                                                                                                                                     >  1:1247224/1‑150 (MQ=255)

 taAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCATAGCGGCTCGCCCTTCGGGCCGATGCGAAAGCAACGTTATCATCACTAGT‑GCCTGCGGTAAATTCCGCTACCTCTTCGATACCATCATTGCTGAAGATTTCg                                                                                                                                                     >  2:1182024/1‑150 (MQ=17)

   ttgCCATATCATTTATGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                                                                                                   <  1:1833694/148‑1 (MQ=255)

   aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                                                                                                   <  2:2372415/150‑1 (MQ=255)

   aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                                                                                                   >  1:303493/1‑150 (MQ=255)

   aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                                                                                                   >  1:835704/1‑150 (MQ=255)

   aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                                                                                                   >  1:303557/1‑150 (MQ=255)

      ccATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCC‑AGTGGCGTCCGTTAAATTCCCCAACATCTTCGATACATAAATTGCTGAAGATTACgtgaaa                                                                                                                                                >  2:2099421/1‑145 (MQ=14)

        atatCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAata                                                                                                                                              >  2:1112307/1‑150 (MQ=255)

         tatCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAatat                                                                                                                                             >  2:1047134/1‑150 (MQ=255)

          atattttAAGAGATGTTTCATCCGGGAGTATTAGTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATg                                                                                                                                            <  2:1588837/145‑1 (MQ=18)

              aTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGc                                                                                                                                        >  1:635466/1‑150 (MQ=255)

              aTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTAGCGGCGCGCCATACGGGCCGTTGCGAAAGCACCGGTATCCTCCCGAGG‑GCCTGCGGTTGAATCCGCTACCTCTTCGATACCTTCATAGCTGAAGAGTTCGGCGAATAGGGTGc                                                                                                                                        >  2:437912/1‑150 (MQ=17)

               ttAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCa                                                                                                                                       >  1:1003619/1‑150 (MQ=255)

               ttAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCa                                                                                                                                       >  1:2025616/1‑150 (MQ=255)

                   gagaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCg                                                                                                                                   >  2:301160/1‑150 (MQ=255)

                    agaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCgg                                                                                                                                  >  2:1309247/1‑150 (MQ=255)

                    agaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCgg                                                                                                                                  >  2:1309283/1‑150 (MQ=255)

                     gaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCggg                                                                                                                                 >  2:565558/1‑150 (MQ=255)

                     gaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCTTCGGGCCGATGCTAAAGCAACGTTATAATCCCTAGT‑GCCTGCGGTTAATACCGCTACATCTTCTATAAATTCATTGATGAAGATTTCTGAGAATATGGTGCATCCggg                                                                                                                                 >  2:2836465/1‑150 (MQ=17)

                       tGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGggag                                                                                                                               <  2:607268/150‑1 (MQ=255)

                         gTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGggagga                                                                                                                             >  1:61844/1‑150 (MQ=255)

                         gTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGggagga                                                                                                                             >  1:824204/1‑150 (MQ=255)

                                cGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTTCTAAAGCAAAGTTATCATCCCTAGT‑GCCAGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAAc                                                                                                                      <  1:1654845/150‑1 (MQ=255)

                                cGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAAc                                                                                                                      <  1:2653940/150‑1 (MQ=255)

                                cGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAAc                                                                                                                      <  1:2654210/150‑1 (MQ=255)

                                  ggaggaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAAAGTTATCATCCCTAGT‑GCCTGCGGTTAAATCCGATACCTCTTCTATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCAACAGGGAGGATTCGAACCt                                                                                                                    >  2:2932047/1‑150 (MQ=17)

                                      gaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCGGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGa                                                                                                                >  1:1312933/1‑150 (MQ=255)

                                       aTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCGACCTCTTCGATACCTTCATTGCGGAAGGTTTCGGGGAATATGGTGCGTCCGGGAGGATTCGAACCGCCGAc                                                                                                               >  1:283595/1‑150 (MQ=17)

                                            tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTACATTGCTGAAGATTTCGGAGAATATGGTGCAACCGGGAGGATTAGAACCACCGACCGCTc                                                                                                          >  1:2620582/1‑150 (MQ=255)

                                                cTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATACCTAGT‑GCCTGCGGTTAATTACGCTACCTCATCGATACCATCATTGCTGTAGATTTAGGAGAATATGGTTCAACCGGGAGGATTCGAACCTCCGACCGCTCGGtt                                                                                                      >  1:729883/1‑150 (MQ=18)

                                                 ttGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                                                                     >  2:1681589/1‑150 (MQ=255)

                                                 ctGCGGCTCGCCCTTCGGGCCGTTGCTCACGCAACGTTATCCTCCCTCGT‑GCCTGCGGTTAATACCGCTACCTCTTCGATACCTTCATGGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                                                                     <  2:1854963/149‑1 (MQ=11)

                                                 cagCGGCTCGCCCTTCGGGCCGTTGCTCCAGCATCGTTATCATCCCTCGT‑GCCTGCGGTTAATCCCGCTACCTCTTCGATCCCTTCATTGCGGAAGACTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                                                                     <  2:2217200/148‑1 (MQ=17)

                                                  tGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCg                                                                                                    <  1:2119733/150‑1 (MQ=255)

                                                   gCGGCTCGCCCTTCGGGCGTTTTCTCCAGCAACGTTATCATCCCTCGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGt                                                                                                   <  2:697323/150‑1 (MQ=17)

                                                        tCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCg                                                                                              >  2:1329346/1‑150 (MQ=255)

                                                        tCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCg                                                                                              >  2:464096/1‑150 (MQ=255)

                                                           cccTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGt                                                                                           >  1:2241138/1‑150 (MQ=255)

                                                            ccTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTa                                                                                          >  2:1973872/1‑150 (MQ=255)

                                                             cTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCAAGG‑GCCAGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCACCGACCGCTCGGTTCGTAGCCGAGTAc                                                                                         >  1:883530/1‑150 (MQ=255)

                                                                   gCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATc                                                                                   >  2:1343265/1‑150 (MQ=255)

                                                                   gCCGTTGCTAAAGCAACCTTATCAACCCTAGT‑GCCAGCCGTTAAATCCCCTACCACATCGAAACCATCATTGCTGAAGATTTCGGAGAATAAGGTGCATCCCGGAGGATTCCAACCACCCACCGCTCGGTTCGTAGCCCACTACACTATc                                                                                   >  1:2463677/1‑150 (MQ=255)

                                                                        tGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                                                              <  2:455091/150‑1 (MQ=255)

                                                                        tGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                                                              <  2:230585/150‑1 (MQ=255)

                                                                          cTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa                                                                            >  1:480636/1‑150 (MQ=255)

                                                                           tAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                           >  1:2599486/1‑150 (MQ=255)

                                                                            aaaGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGTGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGc                                                                          <  2:1402789/150‑1 (MQ=255)

                                                                            aaaGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGc                                                                          <  2:1003619/150‑1 (MQ=255)

                                                                            aaaGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGc                                                                          <  2:2025616/150‑1 (MQ=255)

                                                                            aaaGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGc                                                                          >  1:1796984/1‑150 (MQ=255)

                                                                            aaaGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGc                                                                          <  2:2203072/150‑1 (MQ=255)

                                                                            aaaGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGc                                                                          <  2:2558702/150‑1 (MQ=255)

                                                                            aaaGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCATCATTGCTGAAGATATCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGc                                                                          <  2:732283/150‑1 (MQ=255)

                                                                                cAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACg                                                                      <  1:1463074/150‑1 (MQ=255)

                                                                                cAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACg                                                                      <  1:2893842/150‑1 (MQ=255)

                                                                                    gTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCCAACCACCGACCGCGAGGTGCGTAGCCCAGTACTCTATCCATCAGAGCTAAGGATg                                                                  >  2:429930/1‑150 (MQ=14)

                                                                                     ttATCATCCCTAGT‑GCCTGCGGTTAAATCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCACCTGAGCTACGGATGc                                                                 >  1:1778324/1‑150 (MQ=255)

                                                                                          atcCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCgg                                                            >  1:1054665/1‑150 (MQ=255)

                                                                                           tcCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTCTCCAGCTGAGCTACGGATGCATCggg                                                           >  2:1975712/1‑150 (MQ=255)

                                                                                           tcCCTAGT‑GCCTGCGGTTAATTCCGCAACCTCTTCGATACCTTCATAGCTGAAGATTACGCGGGATATGCAGCCACCCGGAGGATTCGAACCTCAGCCCGCGCGGTTCGTAGCCGAGTACTCTAGCCAGCTGAGCTACGGATGCGGCggg                                                           >  2:2516371/1‑150 (MQ=11)

                                                                                              cTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGGGAATATGGTGCATCCGGGAGGATTCGAACCTACGACAGCTCGCTTCGTAGCAGAGTACTCTATCCAGATGAGCTACGGATGCAGCGGGaca                                                        >  2:194217/1‑148 (MQ=14)

                                                                                                    gCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATACAGCTGAGCTACGGATGCATCGGGAAatttat                                                   <  1:1553178/150‑1 (MQ=255)

                                                                                                      cTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatttattt                                                 <  2:2753426/150‑1 (MQ=255)

                                                                                                        gCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTa                                               >  1:116918/1‑150 (MQ=255)

                                                                                                         cGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTAc                                              >  1:1632009/1‑150 (MQ=255)

                                                                                                          ggTTAATACCGCTAACTCTTCGATACCTTCATTGCTGAAGCTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTAct                                             <  2:872219/150‑1 (MQ=255)

                                                                                                           gTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActg                                            <  2:620954/150‑1 (MQ=255)

                                                                                                              aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTATTTTActgctg                                         >  1:2675483/1‑150 (MQ=255)

                                                                                                              aaTACCGCTATCTCTTCGATACCTTCATTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgctg                                         <  1:1163075/150‑1 (MQ=9)

                                                                                                               aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa                                        <  2:2887977/150‑1 (MQ=255)

                                                                                                               aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa                                        <  2:824204/150‑1 (MQ=255)

                                                                                                               aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATGTATTTTACTGCTGa                                        >  1:912129/1‑150 (MQ=255)

                                                                                                                ttCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGat                                       >  2:645027/1‑150 (MQ=255)

                                                                                                                          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAc                                                                       >  2:2038039/1‑108 (MQ=35)

                                                                                                                          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAc                                                                       <  1:2038039/108‑1 (MQ=35)

                                                                                                                          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCg                             >  1:2340927/1‑150 (MQ=255)

                                                                                                                            ttCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGct                           >  1:2509526/1‑150 (MQ=255)

                                                                                                                               gATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgct                                          <  1:332748/132‑1 (MQ=18)

                                                                                                                               gATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgct                                          >  2:332745/1‑132 (MQ=18)

                                                                                                                                aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTATTTTACTGCTGATAATGATACCGctacta                       >  1:2921371/1‑150 (MQ=255)

                                                                                                                                   ccTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAg                    <  2:1447447/150‑1 (MQ=255)

                                                                                                                                    cTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACAGCTACTAAAGc                   >  2:59511/1‑150 (MQ=255)

                                                                                                                                    cTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGATGATATTGATACCTCTACTAAAGc                   >  2:1518672/1‑150 (MQ=255)

                                                                                                                                     ttCATTGCTGATGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAACGcc                  <  1:183578/150‑1 (MQ=255)

                                                                                                                                     ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                                                             <  1:240612/107‑1 (MQ=35)

                                                                                                                                     ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGcc                  >  1:1920246/1‑150 (MQ=255)

                                                                                                                                        aTTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCtata               >  1:2508974/1‑150 (MQ=255)

                                                                                                                                        aTTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAATTTACTGCTGATATTGATAACGCGACAAAAGACaga               >  2:700437/1‑148 (MQ=14)

                                                                                                                                          tGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGACCTACGGATGCATCGGGAAATTTATTTTACTGCAGATATTGATACCGCTACTAAAGCCATATc             >  1:357315/1‑150 (MQ=255)

                                                                                                                                           gCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGAtttttt                                  <  2:177164/128‑3 (MQ=11)

                                                                                                                                           gCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGAtttttt                                  >  1:177164/1‑126 (MQ=11)

                                                                                                                                             tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAg          <  1:1973872/150‑1 (MQ=255)

                                                                                                                                             tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAg          <  2:303557/150‑1 (MQ=255)

                                                                                                                                             tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAg          <  1:281680/150‑1 (MQ=255)

                                                                                                                                             tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAg          >  2:2422347/1‑150 (MQ=255)

                                                                                                                                             tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAg          >  2:2422299/1‑150 (MQ=255)

CTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGT‑GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGAT  >  NC_000913/2817901‑2818198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`