Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,993,896 T→A 6.1% intergenic (+40/‑43) ygeI → / → pbl uncharacterized protein/pseudogene, peptidoglycan‑binding enzyme family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,993,8960TA 6.1% 516.2 / 17.6 147intergenic (+40/‑43)ygeI/pbluncharacterized protein/pseudogene, peptidoglycan‑binding enzyme family
Reads supporting (aligned to +/- strand):  ref base T (57/81);  new base A (6/3);  total (63/84)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 155 total aligned reads displayed.

TACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTT  >  NC_000913/2993748‑2994045
                                                                                                                                                    |                                                                                                                                                     
tACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTataata                                                                                                                                                      >  1:412825/1‑150 (MQ=255)
tACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTataata                                                                                                                                                      >  2:2886355/1‑150 (MQ=255)
 aCAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAg                                                                                                                                                     >  2:115056/1‑150 (MQ=255)
 aCAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAg                                                                                                                                                     >  2:52790/1‑150 (MQ=255)
 aCAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAg                                                                                                                                                     >  1:289392/1‑150 (MQ=255)
 aCAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAg                                                                                                                                                     >  1:2737636/1‑150 (MQ=255)
    gATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTAt                                                                                                                                                  >  2:1883051/1‑150 (MQ=255)
    gATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTAt                                                                                                                                                  >  2:1883170/1‑150 (MQ=255)
       cAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTATTATAAAAGTAtctc                                                                                                                                               <  1:619143/150‑1 (MQ=255)
       cAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTAGCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTAtctc                                                                                                                                               <  2:1025331/150‑1 (MQ=255)
        aaaTACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCg                                                                                                                                              <  1:12402/150‑1 (MQ=255)
        aaaTACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCg                                                                                                                                              >  2:464842/1‑150 (MQ=255)
           tACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTc                                                                                                                                           >  2:147982/1‑150 (MQ=255)
           tACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTc                                                                                                                                           >  1:1676804/1‑150 (MQ=255)
             cAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTATTATAAAAGTATCTCGttatg                                                                                                                                         <  1:1001804/150‑4 (MQ=255)
             cAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTATTATAAAAGTATCTCGttatg                                                                                                                                         <  1:2058825/150‑4 (MQ=255)
               aGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCttt                                                                                                                                       >  1:1708438/1‑150 (MQ=255)
                 cATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttca                                                                                                                                     <  2:1337620/150‑1 (MQ=255)
                 cATTTATAGTATCAACATGGATGGCGACTTTTCAAAATCATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttca                                                                                                                                     <  1:2118118/150‑1 (MQ=255)
                      ataGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCaa                                                                                                                                <  1:1370189/150‑1 (MQ=255)
                          tttCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCt                                                                                                                            >  2:1159486/1‑150 (MQ=255)
                          tttCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCt                                                                                                                            >  2:2046051/1‑150 (MQ=255)
                                cATGGATGGCGCCTTTTATATATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAATTTGAGTGTTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCTTTCTATCCTcacaca                                                                                                                      <  2:2406185/150‑1 (MQ=255)
                                   ggatggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGa                                                                                                                   >  1:476368/1‑150 (MQ=255)
                                    gatggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGaa                                                                                                                  >  2:56319/1‑150 (MQ=255)
                                    gatggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGaa                                                                                                                  >  2:56285/1‑150 (MQ=255)
                                     atggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCCCCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGaaa                                                                                                                 <  1:2771531/150‑1 (MQ=255)
                                          gcCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAata                                                                                                            <  1:482929/150‑1 (MQ=255)
                                           ccTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAatat                                                                                                           >  1:1156545/1‑150 (MQ=255)
                                                   aaaTGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCAc                                                                                                   >  2:504241/1‑150 (MQ=255)
                                                        atatGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCaaaa                                                                                              <  2:2287770/150‑1 (MQ=255)
                                                        atatGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCaaaa                                                                                              >  1:2163577/1‑150 (MQ=255)
                                                        atatGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCaaaa                                                                                              <  1:650609/150‑1 (MQ=255)
                                                          atGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGt                                                                                            <  1:1592621/150‑1 (MQ=255)
                                                                   gaagaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCtt                                                                                   >  2:2739203/1‑150 (MQ=255)
                                                                   gaagaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCtt                                                                                   >  2:2542682/1‑150 (MQ=255)
                                                                    aagaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTa                                                                                  >  2:2063708/1‑150 (MQ=255)
                                                                     agaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTaa                                                                                 <  2:1523164/150‑1 (MQ=255)
                                                                     agaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTaa                                                                                 <  1:504935/150‑1 (MQ=255)
                                                                      gaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAAc                                                                                <  2:824940/150‑1 (MQ=255)
                                                                         aaTCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCAt                                                                             <  1:115056/150‑1 (MQ=255)
                                                                         aaTCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCAt                                                                             <  1:2242856/150‑1 (MQ=255)
                                                                         aaTCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCAt                                                                             <  1:52790/150‑1 (MQ=255)
                                                                          aTCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATc                                                                            >  1:1289470/1‑150 (MQ=255)
                                                                          aTCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCACACCAAAAGTTAAATTCTTAACCATc                                                                            >  2:318226/1‑150 (MQ=255)
                                                                            ccTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCtt                                                                          <  2:1196004/150‑1 (MQ=255)
                                                                                tctcACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACg                                                                      <  2:2648421/150‑1 (MQ=255)
                                                                                 ctcACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGt                                                                     <  2:1156545/150‑1 (MQ=255)
                                                                                     ccTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTg                                                                 >  1:2353726/1‑150 (MQ=255)
                                                                                     ccTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTg                                                                 >  1:2353715/1‑150 (MQ=255)
                                                                                     ccTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTg                                                                 >  1:742489/1‑150 (MQ=255)
                                                                                        tACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGtat                                                              <  1:648108/150‑1 (MQ=255)
                                                                                               aaaTTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTAtatttatt                                                       <  2:2682982/150‑1 (MQ=255)
                                                                                                aaTTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATttatta                                                      <  2:214248/150‑1 (MQ=255)
                                                                                                aaTTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATttatta                                                      <  1:777006/150‑1 (MQ=255)
                                                                                                 aTTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTAc                                                     <  2:1280039/150‑1 (MQ=255)
                                                                                                      gTGGTAGTCGATTGACCATATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATTTGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACaaaaa                                                <  2:2697183/150‑1 (MQ=255)
                                                                                                      gTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACaaaaa                                                <  2:2628450/150‑1 (MQ=255)
                                                                                                       tGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAAt                                               >  1:286898/1‑150 (MQ=255)
                                                                                                       tGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAAt                                               >  1:1210750/1‑150 (MQ=255)
                                                                                                       tGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAAt                                               >  1:2326635/1‑150 (MQ=255)
                                                                                                        ggTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATg                                              <  2:2200770/150‑1 (MQ=255)
                                                                                                           aGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCt                                           >  1:1459574/1‑150 (MQ=255)
                                                                                                            gACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAATTATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATGTATTACAAAAATGTCtt                                          >  2:2764770/1‑150 (MQ=255)
                                                                                                            gACGATTGACAAAATAAATCATAACGAGTTACTTTTATAATAGTATCGCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCCTCTTCTCGTTGTGTATATTTATTACAAAGGTGTCtt                                          >  2:1959194/1‑150 (MQ=255)
                                                                                                             aCGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCttt                                         >  1:2401610/1‑150 (MQ=255)
                                                                                                              cGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTc                                        <  2:476368/150‑1 (MQ=255)
                                                                                                               gATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCg                                       <  1:1022557/150‑1 (MQ=255)
                                                                                                               gATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCg                                       <  2:1989164/150‑1 (MQ=255)
                                                                                                                aTTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGc                                      <  2:777338/150‑1 (MQ=255)
                                                                                                                aTTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGc                                      <  1:56319/150‑1 (MQ=255)
                                                                                                                aTTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGc                                      <  2:421612/150‑1 (MQ=255)
                                                                                                                aTTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGc                                      <  2:2520926/150‑1 (MQ=255)
                                                                                                                  tGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCa                                    >  2:794308/1‑150 (MQ=255)
                                                                                                                  tGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCa                                    <  1:393667/150‑1 (MQ=255)
                                                                                                                  tGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCa                                    <  2:1708438/150‑1 (MQ=255)
                                                                                                                    aCCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGc                                  >  1:2651562/1‑150 (MQ=255)
                                                                                                                     ccAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCa                                 <  2:1337717/150‑1 (MQ=255)
                                                                                                                     ccAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCa                                 >  1:97366/1‑150 (MQ=255)
                                                                                                                           aaatCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCtt                           >  1:552235/1‑150 (MQ=255)
                                                                                                                             atCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGc                         <  2:215656/150‑1 (MQ=255)
                                                                                                                              tCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGct                        >  1:2069357/1‑150 (MQ=255)
                                                                                                                              tCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGct                        >  2:1864672/1‑150 (MQ=255)
                                                                                                                              tCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGct                        >  1:2525875/1‑150 (MQ=255)
                                                                                                                                  aaCGAGATACTTTTATATTAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAATATATGCGCTCACCAATAGTTATATTATTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTaa                    <  2:2412187/150‑1 (MQ=255)
                                                                                                                                  aaCGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTaa                    <  1:2421179/150‑1 (MQ=255)
                                                                                                                                     gagaTACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGa                 <  2:1559768/150‑1 (MQ=255)
                                                                                                                                     gagaTACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGa                 <  2:1559787/150‑1 (MQ=255)
                                                                                                                                      agaTACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGaa                >  1:560695/1‑150 (MQ=255)
                                                                                                                                      agaTACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGaa                >  1:604106/1‑150 (MQ=255)
                                                                                                                                      agaTACTATTATAAAAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGaa                >  1:35933/1‑150 (MQ=255)
                                                                                                                                      agaTACTATTATAAAAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGaa                >  1:1552509/1‑150 (MQ=255)
                                                                                                                                           cTTTTATAATAGTATCTCGTTCCTTTCATTCACTCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAgg           <  2:2249880/150‑1 (MQ=255)
                                                                                                                                            ttttATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGa          <  2:159714/150‑1 (MQ=255)
                                                                                                                                            ttttATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGa          >  2:2406569/1‑150 (MQ=255)
                                                                                                                                             tttATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGaa         <  2:2467579/150‑1 (MQ=255)
                                                                                                                                             tttATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGaa         <  2:2353726/150‑1 (MQ=255)
                                                                                                                                             tttATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGaa         <  2:2353715/150‑1 (MQ=255)
                                                                                                                                                 taataGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAAt     <  2:2498216/150‑1 (MQ=255)
                                                                                                                                                    taGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGtt  >  1:2018771/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTT  >  NC_000913/2993748‑2994045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: