Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,114,526 G→T 5.0% intergenic (‑174/+24) pppA ← / ← sslE bifunctional prepilin leader peptidase/ methylase/putative secreted and surface‑associated lipoprotein mucinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,114,5260GT 5.0% 820.5 / 23.6 219intergenic (‑174/+24)pppA/sslEbifunctional prepilin leader peptidase/ methylase/putative secreted and surface‑associated lipoprotein mucinase
Reads supporting (aligned to +/- strand):  ref base G (104/104);  new base T (6/5);  total (110/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 223 total aligned reads displayed.

TGCTGGATGCGGCGTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCAT  >  NC_000913/3114377‑3114675
                                                                                                                                                     |                                                                                                                                                     
 gCTGGATGCGGCGTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGa                                                                                                                                                      <  1:1086916/150‑1 (MQ=255)
 gCTGGATGCGGCGTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGa                                                                                                                                                      >  2:331910/1‑150 (MQ=255)
   tGGATGCGGCGTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAgc                                                                                                                                                    >  1:2397436/1‑150 (MQ=255)
   tGGATGCGGCGTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAgc                                                                                                                                                    >  1:1805721/1‑150 (MQ=255)
   tGGATGCGGCGTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAgc                                                                                                                                                    >  1:1434102/1‑150 (MQ=255)
      aTGCGGCGTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGcc                                                                                                                                                 <  1:2809002/150‑1 (MQ=255)
           gcgTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATc                                                                                                                                            <  2:121866/150‑1 (MQ=255)
           gcgTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATGTCATGCCGGATGCGGCGCGAGCGCCTTATc                                                                                                                                            >  1:1633478/1‑150 (MQ=255)
            cgTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATcc                                                                                                                                           >  1:545765/1‑150 (MQ=255)
              tAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCgg                                                                                                                                         <  2:1724712/150‑1 (MQ=255)
              tAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCgg                                                                                                                                         <  1:2445112/150‑1 (MQ=255)
                aaaCGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTGCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATAAGGCGCTCGCGCCGCATCCGGca                                                                                                                                       <  2:1917526/150‑2 (MQ=255)
                 aaCGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATAAGGCGCTCGCGCCGCATCCGGcat                                                                                                                                      <  2:2087117/150‑3 (MQ=255)
                 aaCGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATAAGGCGCTCGCGCCGCATCCGGcat                                                                                                                                      <  2:1606212/150‑3 (MQ=255)
                  aCGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCTAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTa                                                                                                                                     <  2:651098/150‑1 (MQ=255)
                   cGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTAc                                                                                                                                    <  1:2031739/150‑1 (MQ=255)
                     ccTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACgg                                                                                                                                  >  1:1479768/1‑150 (MQ=255)
                     ccTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACgg                                                                                                                                  <  2:815649/150‑1 (MQ=255)
                      cTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACggg                                                                                                                                 <  1:1575832/150‑1 (MQ=255)
                      cTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACggg                                                                                                                                 >  1:2732784/1‑150 (MQ=255)
                       ttATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGc                                                                                                                                <  1:482382/150‑1 (MQ=255)
                          tCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTa                                                                                                                             >  2:2718277/1‑150 (MQ=255)
                          tCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTa                                                                                                                             <  1:2205412/150‑1 (MQ=255)
                             gTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTc                                                                                                                          <  1:490348/150‑1 (MQ=255)
                             gTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTc                                                                                                                          >  2:379472/1‑150 (MQ=255)
                                 tACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATCAGCAGGCGCATCGGGCAAATGTGGTAACCCGGTGCGCCTTAGGTCATGCCGGAGGCGGCGCGAGCGCCGGCGCCGGCCTACGGGCTGACTCGGCa                                                                                                                      >  1:1984485/1‑150 (MQ=255)
                                 tACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCa                                                                                                                      <  1:1271037/150‑1 (MQ=255)
                                 tACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCa                                                                                                                      >  2:450381/1‑150 (MQ=255)
                                        gtCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCt                                                                                                               <  1:1872748/150‑1 (MQ=255)
                                         tCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCtt                                                                                                              <  1:146109/150‑1 (MQ=255)
                                          cTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTa                                                                                                             >  1:2172342/1‑150 (MQ=255)
                                           tGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTAt                                                                                                            <  2:1251367/150‑1 (MQ=255)
                                           tGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTAt                                                                                                            >  1:1162620/1‑150 (MQ=255)
                                                gcgcAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCg                                                                                                       >  1:2824861/1‑150 (MQ=255)
                                                   cAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTa                                                                                                    >  1:2846055/1‑150 (MQ=255)
                                                     aataatCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAAc                                                                                                  <  2:2777970/150‑1 (MQ=255)
                                                          tCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGAt                                                                                             >  1:376652/1‑150 (MQ=255)
                                                          tCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGAt                                                                                             >  2:33410/1‑150 (MQ=255)
                                                           cgcgGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGAtt                                                                                            <  1:444003/150‑1 (MQ=255)
                                                             cgGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTaa                                                                                          <  2:1519789/150‑1 (MQ=255)
                                                             cgGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTaa                                                                                          <  2:1380133/150‑1 (MQ=255)
                                                             cgGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTaa                                                                                          <  2:832320/150‑1 (MQ=255)
                                                               gCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCTCCTTATTTCATGCGGGATGCGGAGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATg                                                                                        <  2:1776287/150‑1 (MQ=255)
                                                               gCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATg                                                                                        <  2:929182/150‑1 (MQ=255)
                                                                  ttCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGtt                                                                                     >  2:2749785/1‑150 (MQ=255)
                                                                      ccGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCg                                                                                 >  1:1215204/1‑150 (MQ=255)
                                                                      ccGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCg                                                                                 >  2:1208933/1‑150 (MQ=255)
                                                                         tCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTc                                                                              <  2:2106566/150‑1 (MQ=255)
                                                                             tAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTg                                                                          >  2:1657349/1‑150 (MQ=255)
                                                                                gCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTc                                                                       <  2:2818168/150‑1 (MQ=255)
                                                                                gCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTc                                                                       >  1:1719576/1‑150 (MQ=255)
                                                                                gCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTc                                                                       >  1:69248/1‑150 (MQ=255)
                                                                                  ccGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCg                                                                     >  2:241252/1‑150 (MQ=255)
                                                                                   cGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCgg                                                                    >  1:339909/1‑150 (MQ=255)
                                                                                   cGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCgg                                                                    >  1:1080052/1‑150 (MQ=255)
                                                                                   cGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCgg                                                                    >  1:2522605/1‑150 (MQ=255)
                                                                                    gATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGt                                                                   >  2:503188/1‑150 (MQ=255)
                                                                                        aGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCg                                                               >  1:1561557/1‑150 (MQ=255)
                                                                                         gCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCgg                                                              >  2:645013/1‑150 (MQ=255)
                                                                                         gCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCgg                                                              <  2:545765/150‑1 (MQ=255)
                                                                                         gCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCgg                                                              >  2:2059903/1‑150 (MQ=255)
                                                                                         gCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCgg                                                              >  2:1059620/1‑150 (MQ=255)
                                                                                           aGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCa                                                            >  2:1476726/1‑150 (MQ=255)
                                                                                              cgcATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGAt                                                         >  2:1649949/1‑150 (MQ=255)
                                                                                                 aTCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGa                                                      <  1:833825/150‑1 (MQ=255)
                                                                                                  tCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAg                                                     <  1:2810688/150‑1 (MQ=255)
                                                                                                  tCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAg                                                     <  2:2629296/150‑1 (MQ=255)
                                                                                                  tCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAg                                                     >  2:2275026/1‑150 (MQ=255)
                                                                                                     ggCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGa                                                  <  2:1411426/150‑1 (MQ=255)
                                                                                                     ggCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGa                                                  <  2:1433415/150‑1 (MQ=255)
                                                                                                       cAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGAcg                                                >  2:697064/1‑150 (MQ=255)
                                                                                                        aaaTGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGAcgc                                               <  2:2154815/150‑1 (MQ=255)
                                                                                                               ttAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCgtg                                        <  1:241252/150‑1 (MQ=255)
                                                                                                                 aaCCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGtgtt                                      <  1:1424161/150‑1 (MQ=255)
                                                                                                                  aCCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGtgttg                                     >  1:324607/1‑150 (MQ=255)
                                                                                                                  aCCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGtgttg                                     >  1:506162/1‑150 (MQ=255)
                                                                                                                     cGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTaa                                  <  2:2920093/150‑1 (MQ=255)
                                                                                                                     cGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTaa                                  >  2:1242356/1‑150 (MQ=255)
                                                                                                                      ggTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAg                                 >  1:1130691/1‑150 (MQ=255)
                                                                                                                           gcCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGAc                            >  1:2895485/1‑150 (MQ=255)
                                                                                                                             cTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTg                          >  1:358067/1‑150 (MQ=255)
                                                                                                                              ttATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTgg                         >  1:1648414/1‑150 (MQ=255)
                                                                                                                                aTTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCt                       <  2:2111236/150‑1 (MQ=255)
                                                                                                                                  ttCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTTACGCGAGCGTTTTGTAAGCAGACTGGCTca                     <  2:794396/150‑1 (MQ=255)
                                                                                                                                  ttCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTca                     <  1:197405/150‑1 (MQ=255)
                                                                                                                                  ttCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTca                     <  2:2127494/150‑1 (MQ=255)
                                                                                                                                      ggCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACAcc                 >  2:1934291/2‑150 (MQ=255)
                                                                                                                                       gtcGGATAAGGCGCTCGCGCCGCATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTGCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCg                >  2:2178757/3‑150 (MQ=255)
                                                                                                                                       gCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCg                >  1:896862/1‑150 (MQ=255)
                                                                                                                                        ccGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGc               >  2:372133/1‑150 (MQ=255)
                                                                                                                                           gATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCt            >  2:2734756/1‑150 (MQ=255)
                                                                                                                                               cggcgCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGaa        <  2:2824861/150‑1 (MQ=255)
                                                                                                                                                ggcgCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAg       <  1:787243/150‑1 (MQ=255)
                                                                                                                                                ggcgCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAg       <  2:563830/150‑1 (MQ=255)
                                                                                                                                                ggcgCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAg       <  2:463957/150‑1 (MQ=255)
                                                                                                                                                ggcgCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAg       <  2:2895714/150‑1 (MQ=255)
                                                                                                                                                ggcgCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAg       <  1:1957859/150‑1 (MQ=255)
                                                                                                                                                ggcgCGAGCGCCTTATCCGGCCTACGGGCTTACTCGACAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAg       <  2:719428/150‑1 (MQ=255)
                                                                                                                                                 gcgcGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGc      >  1:1060954/1‑150 (MQ=255)
                                                                                                                                                   gcgTGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTc    <  1:2926079/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCTGGATGCGGCGTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCAT  >  NC_000913/3114377‑3114675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑