Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,560,199 A→C 5.0% L244W (TTG→TGG)  ddpB ← D,D‑dipeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,560,1990AC 5.0% 400.9 / 8.4 131L244W (TTG→TGG) ddpBD,D‑dipeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (62/61);  new base C (3/4);  total (65/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01

Only 100 of 134 total aligned reads displayed.

CTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTC  >  NC_000913/1560050‑1560347
                                                                                                                                                     |                                                                                                                                                    
ctacCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCTTTGGGTAGCGCATAACATAAAACGATAAACCAGCCGGGCa                                                                                                                                                      >  2:1655478/1‑150 (MQ=255)
  acCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCACACCCAATACGGTAATCGATGGGATCAACGCAGTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCacg                                                                                                                                                    >  2:1747188/1‑148 (MQ=255)
  acCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAg                                                                                                                                                    >  1:1151898/1‑150 (MQ=255)
   cccATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGc                                                                                                                                                   >  2:1694046/1‑150 (MQ=255)
   cccATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGc                                                                                                                                                   <  2:721205/150‑1 (MQ=255)
      aTGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGc                                                                                                                                                <  2:1903999/150‑1 (MQ=255)
      aTGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGc                                                                                                                                                >  1:795106/1‑150 (MQ=255)
       tGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCCAGCCGCt                                                                                                                                               >  1:1485944/1‑150 (MQ=255)
         cACCCATTCCGGGCCAGGCAAAAACGGTTTCGTTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGAAATACCAGCCGGGCAAGCCGCTgg                                                                                                                                             <  1:1424948/150‑1 (MQ=255)
           cccATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGcc                                                                                                                                           >  1:928454/1‑150 (MQ=255)
           cccATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGcc                                                                                                                                           >  1:1150997/1‑150 (MQ=255)
           cccATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGcc                                                                                                                                           >  2:1732675/1‑150 (MQ=255)
            ccATTCCGGGCCAGGCAAAAACGGATTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCACTACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGccc                                                                                                                                          <  1:317448/150‑1 (MQ=255)
              aTTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTTATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCgg                                                                                                                                        <  1:471419/150‑1 (MQ=255)
               ttCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCggg                                                                                                                                       <  2:1484873/150‑1 (MQ=255)
                  cGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCCAGCGGCTTGCCCggctgg                                                                                                                                    <  2:687279/150‑5 (MQ=255)
                   gggCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGt                                                                                                                                   <  1:741207/150‑1 (MQ=255)
                   gggCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGt                                                                                                                                   >  2:2007228/1‑150 (MQ=255)
                   gggCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGt                                                                                                                                   >  1:2121887/1‑150 (MQ=255)
                    ggCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTa                                                                                                                                  <  1:889805/150‑1 (MQ=255)
                      ccAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACg                                                                                                                                <  1:1271688/150‑1 (MQ=255)
                       cAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGa                                                                                                                               >  2:2213794/1‑150 (MQ=255)
                         ggCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAAt                                                                                                                             <  2:511020/150‑1 (MQ=255)
                          gCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATg                                                                                                                            >  2:654138/1‑150 (MQ=255)
                           cAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGt                                                                                                                           <  2:1293893/150‑1 (MQ=255)
                             aaaaCGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAg                                                                                                                         <  1:1206334/150‑1 (MQ=255)
                             aaaaCGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAg                                                                                                                         >  2:1677587/1‑150 (MQ=255)
                                   gTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATAAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCa                                                                                                                   >  1:1104849/1‑150 (MQ=255)
                                      tCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTc                                                                                                                >  1:2069233/1‑150 (MQ=255)
                                        ggTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGc                                                                                                                                                >  2:842848/1‑116 (MQ=255)
                                        ggTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGc                                                                                                                                                <  1:842848/116‑1 (MQ=255)
                                        ggTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCaa                                                                                                              >  2:851563/1‑150 (MQ=255)
                                         gTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAAt                                                                                                             <  2:541503/150‑1 (MQ=255)
                                         gTGAGCACTGCGCCATACAACAAATCGCCCAGCGCAAAACCCAATACGGTAATCGCTGGGAGCAACGCATTGGGTAGCGCTTAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAAt                                                                                                             <  1:293830/150‑1 (MQ=255)
                                          tGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAAtt                                                                                                            >  2:847835/1‑150 (MQ=255)
                                             gCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGtt                                                                                                         >  2:1725606/1‑150 (MQ=255)
                                             gCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACAAGCCGGGCAAGCCGCTGGACCGGGCGGTACGAATGTAGTCTTCACTCAATTGtt                                                                                                         >  2:424303/1‑150 (MQ=255)
                                               aCTGCGCCATACAACAAATCGCCCAGCTCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTcc                                                                                                       <  1:1945983/150‑1 (MQ=255)
                                               aCTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTcc                                                                                                       <  2:2121088/150‑1 (MQ=255)
                                                 tGCGCCATACAAGAAATCGCCCAGCGACAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTATCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTTGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAg                                                                                                     <  1:1089703/150‑1 (MQ=255)
                                                  gcgcCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGc                                                                                                    >  1:1404906/1‑150 (MQ=255)
                                                         acaacaAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCAGTGGGTAGCGCATAAAATAAATCGATATACCAGCCGGCCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCATTTGTTCCAGCATCGCTg                                                                                             >  2:2015669/1‑150 (MQ=255)
                                                         aaaacaaATCGCCCAGCGACAAAACAAATACGTTAATCGATTGGATCCACGCAGTGGGTAGCGCATAACATAAAACGAAATAAAAGCCTGGCAATACGCTGGCCCGGGCGTTACTAAAGTAGGCTTAACACATTGGTTCATGCAGCGCtt                                                                                             >  2:2015667/3‑149 (MQ=255)
                                                          caacaaATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGa                                                                                            >  1:1855155/1‑150 (MQ=255)
                                                          caacaaATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCAATCAATTGTTCCAGCATCGCTGa                                                                                            >  2:1258189/1‑150 (MQ=255)
                                                          caacaaATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAAAGTAGTCTTCACTCAATTGTTCCAGCATCGCTgc                                                                                            >  1:567790/1‑149 (MQ=255)
                                                           aacaaATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGGACGCATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAg                                                                                           >  1:1978299/1‑150 (MQ=255)
                                                            acaaATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGc                                                                                          <  2:223145/150‑1 (MQ=255)
                                                            acaaATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGc                                                                                          <  1:1870388/150‑1 (MQ=255)
                                                             caaATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCg                                                                                         >  1:2102994/1‑150 (MQ=255)
                                                             caaATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCg                                                                                         <  1:587947/150‑1 (MQ=255)
                                                                          gcCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGgcgagcg                                                                            >  2:1110344/1‑150 (MQ=255)
                                                                            cAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGAc                                                                          <  2:758417/150‑1 (MQ=255)
                                                                            cAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGAc                                                                          <  2:898631/150‑1 (MQ=255)
                                                                            cAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGAc                                                                          <  2:1371168/150‑1 (MQ=255)
                                                                               aCCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAAATGTTCCAGCATCGCTGAGCGGATCTGGCGCGCGCCAAt                                                                       >  2:196893/1‑150 (MQ=255)
                                                                                 ccAATACTGTAATCGATTGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTc                                                                     <  2:1099113/150‑1 (MQ=255)
                                                                                 ccAATACGGTAATCGATGGGATCATCGCATTGGGTAGCGCATAACATATAACGATATACCAGCCGGTCTAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTc                                                                     <  2:164617/150‑1 (MQ=255)
                                                                                 ccAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTc                                                                     <  2:1106296/150‑1 (MQ=255)
                                                                                  cAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAAATGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTcc                                                                    >  1:1927915/1‑150 (MQ=255)
                                                                                   aaTACTGTAATCTATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTccc                                                                   <  2:332333/150‑1 (MQ=255)
                                                                                      aCGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAgg                                                                <  2:206583/150‑1 (MQ=255)
                                                                                         gTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTga                                                             <  1:45319/150‑1 (MQ=255)
                                                                                           aaTCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCGGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTgaac                                                           <  1:38250/150‑1 (MQ=255)
                                                                                            aTCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTgaacg                                                          >  1:667910/1‑150 (MQ=255)
                                                                                               gATGGGATCAACGCATTGGGTAGCGCATAACATAACACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTgaacgaac                                                       <  1:1747188/150‑1 (MQ=255)
                                                                                                    gATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAg                                                  >  2:255697/1‑150 (MQ=255)
                                                                                                        aaCGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGTGCGGATCTGGCGAGCGACAATTCCCACGTGAACGAACGCCAGCGtt                                              >  2:609501/1‑150 (MQ=255)
                                                                                                           gCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGCACGCCAGCGTTAAt                                           >  1:1508387/1‑150 (MQ=255)
                                                                                                            cATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATg                                          <  1:1655478/150‑1 (MQ=255)
                                                                                                                 ggTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCTATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGt                                     >  2:1250160/1‑150 (MQ=255)
                                                                                                                     gcgcATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAaga                                 >  1:2215931/1‑150 (MQ=255)
                                                                                                                        cataacataaAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAagatga                              >  2:2010215/1‑150 (MQ=255)
                                                                                                                         ataacataaAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAagatgag                             <  1:952080/150‑1 (MQ=255)
                                                                                                                         ataacataaAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAagatgag                             <  1:89658/150‑1 (MQ=255)
                                                                                                                          taacataaAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGACCGAACGCCAGCGTTAATGCCGGTAtgatgaga                            >  2:1931671/1‑150 (MQ=255)
                                                                                                                             cataaAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGCGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGt                         >  1:1649795/1‑150 (MQ=255)
                                                                                                                             cataaAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGt                         <  2:145357/150‑1 (MQ=255)
                                                                                                                              ataaAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGGGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGtt                        <  2:1876937/150‑1 (MQ=255)
                                                                                                                               taaAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGTGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTg                       >  1:367835/1‑150 (MQ=255)
                                                                                                                               taaAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGCACGCCAGCGTTAATGCCGGTAAGATGAGATGTTg                       >  2:289923/1‑150 (MQ=255)
                                                                                                                               taaAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTg                       <  2:1404906/150‑1 (MQ=255)
                                                                                                                                 aaaCGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCACAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCa                     >  2:1214620/1‑150 (MQ=255)
                                                                                                                                   aCGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAAc                   >  1:1049487/1‑150 (MQ=255)
                                                                                                                                    cGATATACCAGCCGTGCAAGCCGCGGGCCCGGGCGGGACGAATGTAGTCTTCACTCAATTTTTCCATCATAGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACg                  <  2:1427953/150‑1 (MQ=255)
                                                                                                                                    cGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACg                  <  2:721532/150‑1 (MQ=255)
                                                                                                                                     gATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGc                 <  2:928454/150‑1 (MQ=255)
                                                                                                                                     gATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTGCACTCACTTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGCACGCCAGCGTTAAGGCCTGTATGATGAGATGTTGCAACGc                 >  1:412753/1‑150 (MQ=255)
                                                                                                                                         taCCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTg             <  2:1151898/150‑1 (MQ=255)
                                                                                                                                          aCCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTga            <  1:2007228/150‑1 (MQ=255)
                                                                                                                                           ccAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTgaa           >  2:1291237/1‑150 (MQ=255)
                                                                                                                                             gcccGGGCCAGCGGCTTGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATTGCTGAGCGGCTCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTgaaga         >  1:1733910/3‑150 (MQ=255)
                                                                                                                                              gCCGGTCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGGTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTgaagaa        <  1:418582/150‑1 (MQ=255)
                                                                                                                                               ccGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGaagaag       <  2:2069233/150‑1 (MQ=255)
                                                                                                                                                cGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGCGCGACAATTCCCCGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCACCGCATTGAagaaga      >  1:942960/1‑150 (MQ=255)
                                                                                                                                                 gggCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGAc     <  2:659553/150‑1 (MQ=255)
                                                                                                                                                 gggCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGAc     <  1:851563/150‑1 (MQ=255)
                                                                                                                                                 gggCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGAc     >  2:12915/1‑150 (MQ=255)
                                                                                                                                                    cAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTc  >  2:2012476/1‑150 (MQ=255)
                                                                                                                                                    cAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAAAGCCGGTAAGATGAGATGAAGCACCGCATTGATGAAGCCTTc  >  2:117288/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTC  >  NC_000913/1560050‑1560347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: