Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,568,915 G→A 5.0% intergenic (‑92/+39) yddW ← / ← gadC liprotein, glycosyl hydrolase homolog/glutamate:gamma‑aminobutyric acid antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,568,9150GA 5.0% 447.5 / 6.3 138intergenic (‑92/+39)yddW/gadCliprotein, glycosyl hydrolase homolog/glutamate:gamma‑aminobutyric acid antiporter
Reads supporting (aligned to +/- strand):  ref base G (76/54);  new base A (5/2);  total (81/57)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 141 total aligned reads displayed.

CAACTAGTATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGC  >  NC_000913/1568766‑1569063
                                                                                                                                                      |                                                                                                                                                    
cAACTAGTATCGCTGTTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATg                                                                                                                                                      <  1:746920/150‑1 (MQ=255)
cAACTAGTATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATg                                                                                                                                                      >  1:795187/1‑150 (MQ=255)
cAACTAGTATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATg                                                                                                                                                      >  2:468911/1‑150 (MQ=255)
     aGTATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTa                                                                                                                                                 >  1:929039/1‑150 (MQ=255)
        aTCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGCTTTCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGCGCGATGAATTATCg                                                                                                                                              >  2:1988340/1‑150 (MQ=255)
          cGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCt                                                                                                                                            <  1:39933/150‑1 (MQ=255)
           gCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTAACGCta                                                                                                                                           >  2:1946166/1‑149 (MQ=255)
           gCTGGTCTTCTAATCCTTAATTTCTAGTTTCGGGAGCAGATATCCATATGTGATGGATTCCGGTAAAAAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGAAAGCACGAAAAACTGAGCGATAAATTATAGcaa                                                                                                                                           >  2:1550220/1‑148 (MQ=255)
             tGGTCTTCTAATCGTTACTTTCTTGTTTCGGGCGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCTTACTTATCCCCGTTAAGTCAATTCGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTccc                                                                                                                                         <  2:1710359/150‑1 (MQ=255)
             tGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTccc                                                                                                                                         >  2:598442/1‑150 (MQ=255)
             tGGTCTTCAAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATAATTCATCGCTccc                                                                                                                                         <  2:826778/150‑1 (MQ=255)
              ggTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAATCACGAAAAAGGGAGCGATTAATTATCGCTCCCt                                                                                                                                        <  2:1038152/150‑1 (MQ=255)
                   tctAATCGTTAATTACTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAACAGGGAGCGATGAATTATCGCTCCCTTGTCt                                                                                                                                   >  1:1496027/1‑150 (MQ=255)
                    ctAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCtt                                                                                                                                  >  2:718050/1‑150 (MQ=255)
                       aTCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTtata                                                                                                                               <  1:1772924/150‑1 (MQ=255)
                        tCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATaa                                                                                                                              >  1:1173852/1‑150 (MQ=255)
                          gTTAATTTCTTGTTTCGGGAGCAGAAATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTTCTTATCCCCGTTAAGTCAATACGACAGAAAGCACAAAAAACTGAGCGATGAATTATCGCTCCCTTGTCTTATAccc                                                                                                                            >  1:366286/1‑150 (MQ=255)
                           ttAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCa                                                                                                                           >  2:1357234/1‑150 (MQ=255)
                              aTTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCAtta                                                                                                                        >  2:1845828/1‑149 (MQ=255)
                               tttCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCa                                                                                                                       >  2:2099199/1‑150 (MQ=255)
                                  cTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGAc                                                                                                                    >  2:1722806/1‑150 (MQ=255)
                                   ttGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAAACATTCAGACa                                                                                                                   >  2:754331/1‑150 (MQ=255)
                                    tGTTTCGGTCGCAGATATCCATATGTGCTGTTTGCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAACTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCTTTCAGACAt                                                                                                                  <  1:207487/150‑1 (MQ=255)
                                     gTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATg                                                                                                                 <  1:1357234/150‑1 (MQ=255)
                                      tttCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATgg                                                                                                                <  2:627871/150‑1 (MQ=255)
                                       ttCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGAGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTTTCTTATAACCATTCAGACAAGGt                                                                                                               >  1:559071/1‑150 (MQ=255)
                                        tagggAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGtt                                                                                                              >  1:106075/3‑150 (MQ=255)
                                         cGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTa                                                                                                             >  1:1183223/1‑150 (MQ=255)
                                           ggAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAgt                                                                                                           <  2:736656/150‑1 (MQ=255)
                                            gAGCAGATATCCATTTGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAgtg                                                                                                          <  2:681213/150‑1 (MQ=255)
                                                aGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTc                                                                                                      >  2:1295939/1‑150 (MQ=255)
                                                aGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTc                                                                                                      >  2:1524064/1‑150 (MQ=255)
                                                 gATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCt                                                                                                     >  1:1233073/1‑150 (MQ=255)
                                                   tatCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTg                                                                                                   <  1:1410917/150‑1 (MQ=255)
                                                     tCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCATTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGtc                                                                                                 <  1:1783258/150‑1 (MQ=255)
                                                          atGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGtcattca                                                                                            >  1:1693185/1‑150 (MQ=255)
                                                           tgtgCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGtcattcat                                                                                           <  1:5043/150‑1 (MQ=255)
                                                            gtgCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATtcatc                                                                                          >  1:1905874/1‑150 (MQ=255)
                                                            gtgCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATtcatc                                                                                          >  1:1270144/1‑150 (MQ=255)
                                                              gCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATcac                                                                                        >  1:1148140/1‑150 (MQ=255)
                                                              gCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATcac                                                                                        >  1:1013913/1‑150 (MQ=255)
                                                              gCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCCTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATcac                                                                                        <  2:1668654/150‑1 (MQ=255)
                                                              gCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCCTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATcac                                                                                        <  2:888589/150‑1 (MQ=255)
                                                              gCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAAATATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATcac                                                                                        <  2:808886/150‑1 (MQ=255)
                                                               cTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATcac                                                                                        <  1:875126/150‑1 (MQ=255)
                                                                 ggTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAat                                                                                     >  2:184684/1‑150 (MQ=255)
                                                                 ggTTTCCGGTAAACAGATGTGCGATCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAat                                                                                     <  1:826022/150‑1 (MQ=255)
                                                                  gTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAata                                                                                    >  1:2099810/1‑150 (MQ=255)
                                                                  gTTTCCGGTAAACAGATGTACGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAata                                                                                    >  1:1747217/1‑150 (MQ=255)
                                                                    ttCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAAtata                                                                                  >  2:1166573/1‑150 (MQ=255)
                                                                       cGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgtg                                                                               >  1:772149/1‑150 (MQ=255)
                                                                       cGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgtg                                                                               >  1:748210/1‑150 (MQ=255)
                                                                        ggTAAACAGATGTGCTCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAATAAGGGAGCGATGAATTATCGCTCCCTTGTCTTTTATCCATTCATACATGGTTAGTGTTTCTTGTCTTTCATCACAATATAgtgt                                                                              <  1:1150404/150‑1 (MQ=255)
                                                                        ggTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAACCACGAAAAAGGGAGCGATGAATTATCGCTCCCTAGTATTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCAAAATATAgtgt                                                                              >  2:1998863/1‑150 (MQ=255)
                                                                          tAAACAGATGTGCGCGCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtgg                                                                            <  1:1413729/150‑1 (MQ=255)
                                                                            aaCAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggtg                                                                          >  2:1427568/1‑150 (MQ=255)
                                                                                gATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAAcc                                                                      >  2:494294/1‑149 (MQ=255)
                                                                                  tgtgCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTg                                                                    <  1:606677/150‑1 (MQ=255)
                                                                                  tgtgCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTg                                                                    >  2:1718523/1‑150 (MQ=255)
                                                                                  tgtgCGCTCATTCTCGTACTTATCCACGTTAAGTCAATACGACAGCAATCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTCTAACCAATCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTTAACGTg                                                                    <  2:328840/150‑1 (MQ=255)
                                                                                    tgCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCa                                                                  >  2:557579/1‑150 (MQ=255)
                                                                                       gcTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcgc                                                               >  1:1012837/1‑150 (MQ=255)
                                                                                          cATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCggg                                                            >  1:1623314/1‑150 (MQ=255)
                                                                                                cGTACTTATCCCCGTTAAGTCAATACGACATCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAgg                                                      <  1:546267/150‑1 (MQ=255)
                                                                                                  tACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaa                                                    >  2:2137419/1‑150 (MQ=255)
                                                                                                      tATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGTCATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGaagaag                                                >  2:1054651/1‑150 (MQ=255)
                                                                                                       aTCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGt                                               <  2:1246594/150‑1 (MQ=255)
                                                                                                         ccccGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATACCCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGa                                             >  2:504671/1‑150 (MQ=255)
                                                                                                          cccGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCACACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGaa                                            >  2:1631383/1‑149 (MQ=255)
                                                                                                                 aGTCAATTCGACAGCAAGCACGAAAAAGGGATCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTATTGTCATTCATCACAATATAGTGTTGTGAACGTGCACGCTGGTGCAGGAAGAAGTGACCTTTTgg                                     <  1:1600451/150‑1 (MQ=255)
                                                                                                                 aGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTgg                                     <  2:1143884/150‑1 (MQ=255)
                                                                                                                  gTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGt                                    >  1:791296/1‑150 (MQ=255)
                                                                                                                    cAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGc                                  <  2:479502/150‑1 (MQ=255)
                                                                                                                    cAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGc                                  >  2:1986686/1‑150 (MQ=255)
                                                                                                                    cAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTACAACCTGTCAGACATGGTTAGTGTTTCTTGTCAGTCATCACAATATAGTGGGGTGCACGTGCACGCGGGTGCAGGAAGAAGTGCCCGTTTGGTgg                                  >  2:878156/1‑149 (MQ=255)
                                                                                                                       tACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGtt                               <  2:1218197/150‑1 (MQ=255)
                                                                                                                         cGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTGCTTGTCATTCATCACAATATAGGGGGGTGAACGTGCACGCGGGTGCAGGATGAAGTGACCTTTTGGTGCGTTCt                             <  1:279703/150‑1 (MQ=255)
                                                                                                                           aCAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGAGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTGTGa                           >  1:304244/1‑150 (MQ=255)
                                                                                                                             agcaagcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACt                         >  2:2149139/1‑150 (MQ=255)
                                                                                                                              gcaagcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTg                        >  1:384663/1‑150 (MQ=255)
                                                                                                                                aagcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGtt                      >  1:1338944/1‑150 (MQ=255)
                                                                                                                                 agcaCGAAATAGTGAGAGATGTTTTTTCGCTCTGTTGTCTTATTATCATTCAGACATGGTTAGTGTTGCTTGTCATTCATCACAATATAGTGTGTGAACGTGCTCGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGa                    <  1:331630/150‑1 (MQ=255)
                                                                                                                                  gcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGACCGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGa                    >  2:290458/1‑150 (MQ=255)
                                                                                                                                   caCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACATTATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTGCTGACTGTTGAt                   <  1:1166573/150‑1 (MQ=255)
                                                                                                                                   caCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGGGACCTTTTGGTGCGTTCTGACTGTTGAt                   <  1:708517/150‑1 (MQ=255)
                                                                                                                                    ccGAAAAAGGGAGCGATGAATTATCGCGCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGGGTGGTGAACGTGCACGCGGGGGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGAtt                  <  1:536446/149‑1 (MQ=255)
                                                                                                                                     cGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTg                 <  2:1882475/150‑1 (MQ=255)
                                                                                                                                          caaGGGAGCGATAATTCATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGAGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTc             >  2:1743173/2‑150 (MQ=255)
                                                                                                                                            aGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCa           <  1:1295939/150‑1 (MQ=255)
                                                                                                                                              ggAGCGATAATTCATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCaga         >  1:1549109/1‑150 (MQ=255)
                                                                                                                                               gAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCagag        <  2:1747217/150‑1 (MQ=255)
                                                                                                                                                aGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGGGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGt       <  1:1711559/150‑1 (MQ=255)
                                                                                                                                                 gCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTg      <  1:803449/150‑1 (MQ=255)
                                                                                                                                                 gCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTg      <  1:198774/150‑1 (MQ=255)
                                                                                                                                                  cGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGa     >  1:21295/1‑150 (MQ=255)
                                                                                                                                                  cGATGAATTAGCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGGGTGGTGAACGTGCACGCGGGGGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGa     <  2:48023/150‑1 (MQ=255)
                                                                                                                                                   gATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGAc    >  2:679702/1‑150 (MQ=255)
                                                                                                                                                   gATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGAc    >  1:881422/1‑150 (MQ=255)
                                                                                                                                                     tGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGCAGTGCCCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGc  >  2:1157379/1‑150 (MQ=255)
                                                                                                                                                     tGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGGGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGc  <  1:1546290/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
CAACTAGTATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGC  >  NC_000913/1568766‑1569063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: