Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,602,236 A→T 5.7% Q249H (CAA→CAT lsrA → autoinducer 2 import ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,602,2360AT 5.7% 372.0 / 5.2 124Q249H (CAA→CATlsrAautoinducer 2 import ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (61/55);  new base T (4/4);  total (65/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

CCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTGGCTGGGCTGGTGG  >  NC_000913/1602090‑1602384
                                                                                                                                                  |                                                                                                                                                    
ccGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTAAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCaaaaa                                                                                                                                                   <  1:1848586/150‑1 (MQ=255)
   gAAATTCGCCAGATTGCCGAGCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTa                                                                                                                                                <  2:1553164/150‑1 (MQ=255)
    aaaTTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTAt                                                                                                                                               <  2:847544/150‑1 (MQ=255)
         cGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGATAAATCGCTCTCTGCCAGCCAAAAATTATGGCTg                                                                                                                                          >  1:1910968/1‑150 (MQ=255)
           ccAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATAATTTTTGGCTggc                                                                                                                                        <  1:23306/150‑2 (MQ=255)
           ccAGATTGCAGATCGAATTAGCGTGATGCGCGACGGAACCTTCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATAATTTTTGGCTggc                                                                                                                                        <  2:817911/150‑2 (MQ=255)
            cAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAg                                                                                                                                       <  1:1588158/150‑1 (MQ=255)
             aGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATAATTTTTGGCTggcag                                                                                                                                      <  2:1597213/150‑4 (MQ=255)
              gATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGtt                                                                                                                                     >  2:274158/1‑150 (MQ=255)
                 tGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTAcc                                                                                                                                  <  1:1509198/150‑1 (MQ=255)
                 tGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTAcc                                                                                                                                  <  2:2168547/150‑1 (MQ=255)
                   ccGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAACACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACTGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGttcactg                                                                                                                                >  1:1107573/1‑145 (MQ=255)
                   ccGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTg                                                                                                                                >  2:190500/1‑150 (MQ=255)
                   ccGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTg                                                                                                                                <  1:1625851/150‑1 (MQ=255)
                   ccGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTg                                                                                                                                <  2:421778/150‑1 (MQ=255)
                    cGATCGAATTAGCGTGATGCGCGACGTAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTgg                                                                                                                               <  1:1937839/150‑1 (MQ=255)
                     gATCGAATAAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGt                                                                                                                              >  1:1351832/1‑150 (MQ=255)
                       tCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCTAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTTCGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTaa                                                                                                                            <  1:1062922/150‑1 (MQ=255)
                       tCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAAATATGGCTGGAGTTACCTGGTaa                                                                                                                            >  1:1106972/1‑150 (MQ=255)
                        cGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAAc                                                                                                                           <  1:1006386/150‑1 (MQ=255)
                         gAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATAAATTATGGCTGGAGTTACCTGGTAAcc                                                                                                                          >  2:559459/1‑150 (MQ=255)
                             tAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGccc                                                                                                                      <  1:1886570/150‑1 (MQ=255)
                               gCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCTAAACCAGCCAACTGTCTACCGACGACATTAATCAGGCCATCACCCCAGCCGATCGGGAAAAATCGCGGGCTGCCTGCCAAAAATTATGCCTGGAGTTACCTGCTAACCGCCcac                                                                                                                    >  1:1182772/1‑150 (MQ=255)
                               gCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCcac                                                                                                                    <  1:962657/150‑1 (MQ=255)
                               gCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCcac                                                                                                                    <  2:657746/150‑1 (MQ=255)
                                 gTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCCGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCacaa                                                                                                                  >  1:16187/1‑150 (MQ=255)
                                    aTGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACAt                                                                                                               >  1:497120/1‑150 (MQ=255)
                                                 ccATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGc                                                                                                  >  1:2054348/1‑150 (MQ=255)
                                                 ccATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTAAGGCCATCACCCCAGCGGTACGGGAATAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGc                                                                                                  >  2:2130180/1‑150 (MQ=255)
                                                 ccATAGCATTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACAGAATTAAGGCCATCACCACAGCGGTACGGGAAAAGTCGCTGTCTGCCAGGCAAAATTGATCCATGAAGTTAGATGGTAACCGAACACTACAGGACTCCGGAACGc                                                                                                  >  2:887859/1‑150 (MQ=255)
                                                  cATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAAAGcc                                                                                                 <  1:1277410/150‑1 (MQ=255)
                                                  cATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTATGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGcc                                                                                                 >  2:1700166/1‑150 (MQ=255)
                                                   aTCGCCTTAAGCGGCAAACCCAGCGAACTGTCTACCGACGAGATTATTCAGGCCATCACCCCAGCGCTACCGGAAAACTCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTACCCGCCCACAACATGCCGCCGGAacgacg                                                                                                >  1:296958/1‑150 (MQ=255)
                                                      cccTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACTGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTg                                                                                             <  2:263823/149‑1 (MQ=255)
                                                         ttAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTg                                                                                          >  1:2179110/1‑150 (MQ=255)
                                                         ttAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTg                                                                                          <  1:1808855/150‑1 (MQ=255)
                                                          tAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGa                                                                                         >  1:2089516/1‑150 (MQ=255)
                                                          tAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGa                                                                                         >  2:2210989/1‑150 (MQ=255)
                                                            aGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCCCCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCGAAAAATTATGGCTGGAGTTCCCTGGTAACCGCCCACAACATGCCGCCGGACCGCCGGTGGTGcca                                                                                       >  2:127510/1‑150 (MQ=255)
                                                             gCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACACCATGCCGCCGGACCGCCGGTGCTGacac                                                                                      >  1:767542/1‑150 (MQ=255)
                                                             gCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGacac                                                                                      >  1:1283648/1‑150 (MQ=255)
                                                               ggCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCCGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTACCCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTg                                                                                    >  1:1754716/1‑150 (MQ=255)
                                                               ggCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTg                                                                                    <  1:48833/150‑1 (MQ=255)
                                                                gCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTgg                                                                                   <  2:229514/150‑1 (MQ=255)
                                                                gCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTgg                                                                                   <  2:405280/150‑1 (MQ=255)
                                                                              cTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGGGCTGACACTGGAAAATCTGACAGGc                                                                     >  1:2173476/1‑150 (MQ=255)
                                                                                 tCTACCGACGACATTATTCAGGCCACCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGaa                                                                  <  2:1532399/150‑1 (MQ=255)
                                                                                  cTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCACTCTGCCAGCCAAAAATTATGGCAGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAg                                                                 >  1:636704/1‑150 (MQ=255)
                                                                                   tACCGACGACATTATTCCGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAgg                                                                >  2:681371/1‑150 (MQ=255)
                                                                                   tACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAgg                                                                >  1:1708579/1‑150 (MQ=255)
                                                                                    acagacgacATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGCCCGGCGAAGGt                                                               >  1:362834/4‑150 (MQ=255)
                                                                                      cgacgaCATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGttt                                                             >  1:338481/1‑150 (MQ=255)
                                                                                        acgacATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGTAAAATCTGACCGGCGAAGGTTTct                                                           <  1:274158/150‑2 (MQ=255)
                                                                                           acATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGa                                                        >  1:13664/1‑150 (MQ=255)
                                                                                           acATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGaa                                                       >  2:786518/1‑150 (MQ=255)
                                                                                              ttattCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATg                                                     <  1:716169/150‑1 (MQ=255)
                                                                                                  tCAGGCCATCACCCCAGCGGTACGGGAAAAATCTCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAAGCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAg                                                 <  1:1762027/150‑1 (MQ=255)
                                                                                                   cAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTCCCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGCCCGGCGAAGCTTTCAGGAATGTCAGc                                                >  1:513345/1‑150 (MQ=255)
                                                                                                    aGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGcc                                               <  1:617706/150‑1 (MQ=255)
                                                                                                    aGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAAACGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGct                                               <  2:1351832/150‑2 (MQ=255)
                                                                                                     ggCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCt                                              <  1:170055/150‑1 (MQ=255)
                                                                                                      gCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACCACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTg                                             >  2:927439/1‑150 (MQ=255)
                                                                                                       ccATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGa                                            >  1:1594614/1‑150 (MQ=255)
                                                                                                             ccccAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAAATATGGCTGGAGTTACCTGGAAACCGCCCACAACATGCCGCCGGACCGCCGGTGCTCACACTGGATAATCTGACCGGCGAAGGATACAGGAATGTCCCCCTGACGCTcc                                      >  1:430002/1‑149 (MQ=255)
                                                                                                             ccccAGCGGTACGGGAAAAATCGCTCTCTGCCAGACAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGTAATGTCAGCCTGACGCTCa                                      >  2:359174/1‑150 (MQ=255)
                                                                                                               ccAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAAt                                    >  2:89540/1‑150 (MQ=255)
                                                                                                                cAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATg                                   >  1:2060780/1‑150 (MQ=255)
                                                                                                                    ggTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCgg                               >  2:1143398/1‑150 (MQ=255)
                                                                                                                    ggTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCAGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCgg                               >  1:1546572/1‑150 (MQ=255)
                                                                                                                    ggTAAGGGAAAAAGCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCgg                               <  2:1329342/150‑1 (MQ=255)
                                                                                                                      tACGGGAAAAATCGCTCTCTGCCAGCCAAAAAATATGGCTGGATTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATTCCGgag                             <  2:1146864/150‑1 (MQ=255)
                                                                                                                          ggAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACCACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGCCGCTCACTGCCGGAGAAAt                         >  1:1509606/1‑150 (MQ=255)
                                                                                                                          ggAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACTCTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAAt                         >  2:1178988/1‑150 (MQ=255)
                                                                                                                          ggAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAAt                         >  1:1993189/1‑150 (MQ=255)
                                                                                                                          ggAAAAATCGCTCTCTGCCAGCCAAAAAATATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAAt                         >  1:872491/1‑150 (MQ=255)
                                                                                                                           gAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAAtt                        >  1:781160/1‑150 (MQ=255)
                                                                                                                            aaaaaTCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTc                       <  2:46597/150‑1 (MQ=255)
                                                                                                                              aaaTCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTg                     >  2:832683/1‑150 (MQ=255)
                                                                                                                              aaaTCGCTCTCTGCCACGCAAAATATATGGCTGGAGTAACCTGGAAACCGCCCACACCATGCCGCCGGAACGCCGGTGCTGACACTGGAAAAACTGCCCGGCGATGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTg                     <  2:1340406/150‑1 (MQ=255)
                                                                                                                                aTCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTggg                   >  2:2194262/1‑150 (MQ=255)
                                                                                                                                    ctctctGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCctg               >  1:956766/1‑150 (MQ=255)
                                                                                                                                       tctGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCctggct            <  2:2067164/150‑1 (MQ=255)
                                                                                                                                        ctGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCctggctg           <  2:2089516/150‑1 (MQ=255)
                                                                                                                                        ctGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGGTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCctggctg           <  1:952393/150‑1 (MQ=255)
                                                                                                                                        actccagccaTAATTTTTGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCctggctg           >  1:1865117/4‑150 (MQ=255)
                                                                                                                                         tGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGACGCCGGAACGACGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCctggctgg          >  2:192444/1‑150 (MQ=255)
                                                                                                                                          tccagccaTAATTTTTGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTggctggg         >  2:1905295/2‑150 (MQ=255)
                                                                                                                                          gccagccaAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGCCCGGCGAAGGTTTCAGGAATGTCAGCCTGCCGCTCAATGCCGGAGAAATTGTGGGCCTggctggg         >  1:578762/1‑150 (MQ=255)
                                                                                                                                          gccagccaAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTggctggg         >  2:1784120/1‑150 (MQ=255)
                                                                                                                                          gccagccaAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCGCAATGCCGGAGAAATTCTGGGCCTggctggg         >  1:2198201/1‑150 (MQ=255)
                                                                                                                                           ccagccaTAATTTTTGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTggctgggc        >  1:2130340/1‑150 (MQ=255)
                                                                                                                                           ccagccaAAAATTCAGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTggctgggc        <  2:1475568/150‑1 (MQ=255)
                                                                                                                                            cagccaAAATTTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGCACGCCGGTGCGGACACTGGAAACTCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCGCAATGCCGGAGAAATTCTGGGCCTggctgggct       <  2:317917/150‑1 (MQ=255)
                                                                                                                                            cagccaAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTggctgggct       <  2:1214873/150‑1 (MQ=255)
                                                                                                                                            cagcaaATAATTATGCGTGGATTTACCCGGTAACCGCCCACCACCTGCCGCCGGAACGCCGGTGCTGACACTCGAAAATCTGACCGGCGAAGGTTTCAGGACTGTCAGCCTGACGCTCAATGCCGCAGAAATTCTGGGCCTggctgggct       <  2:218398/150‑1 (MQ=255)
                                                                                                                                             agcaaAACATTATGGCTGGAGTTACCTGGTAACAGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCGGCCGGACGCTCAATGCCGGAGACATTCTGGGCCTggctcggctg      <  2:1396434/150‑1 (MQ=255)
                                                                                                                                              gccaAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTGgctgggctgg     >  2:418738/1‑150 (MQ=255)
                                                                                                                                               ccaAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTGGCTGGGCtggt    >  1:634455/1‑150 (MQ=255)
                                                                                                                                                caAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGCCCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTGGCTGGGCtggtg   >  2:59343/1‑150 (MQ=255)
                                                                                                                                                caAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTTACGCTCAATGCAGTAGAAATTCTGGGCCTGGCTGGGCtggtg   >  2:126252/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTGGCTGGGCTGGTGG  >  NC_000913/1602090‑1602384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: