Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,602,242 A→T 6.2% L251F (TTA→TTT lsrA → autoinducer 2 import ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,602,2420AT 6.2% 352.4 / 9.9 126L251F (TTA→TTTlsrAautoinducer 2 import ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (62/55);  new base T (4/4);  total (66/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.22e-01

Only 100 of 128 total aligned reads displayed.

GAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTGGCTGGGCTGGTGGGGGCCGG  >  NC_000913/1602093‑1602391
                                                                                                                                                     |                                                                                                                                                     
gAAATTCGCCAGATTGCCGAGCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTa                                                                                                                                                       <  2:1553164/150‑1 (MQ=255)
 aaaTTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTAt                                                                                                                                                      <  2:847544/150‑1 (MQ=255)
      cGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGATAAATCGCTCTCTGCCAGCCAAAAATTATGGCTg                                                                                                                                                 >  1:1910968/1‑150 (MQ=255)
        ccAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATAATTTTTGGCTggc                                                                                                                                               <  1:23306/150‑2 (MQ=255)
        ccAGATTGCAGATCGAATTAGCGTGATGCGCGACGGAACCTTCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATAATTTTTGGCTggc                                                                                                                                               <  2:817911/150‑2 (MQ=255)
        ccAGATGGCCGATCGAATTCGCGTGCTGCGCGACGGAACCATCGCCTTAAGCGGCAAACCCAGCGAACTGTCTACCGACGACATTAATCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATAATTTTTGGCTggc                                                                                                                                               <  1:2139752/150‑2 (MQ=255)
         cAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAg                                                                                                                                              <  2:1839907/150‑1 (MQ=255)
         cAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAg                                                                                                                                              <  1:1588158/150‑1 (MQ=255)
          aGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATAATTTTTGGCTggcag                                                                                                                                             <  2:1597213/150‑4 (MQ=255)
           gATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGtt                                                                                                                                            >  2:274158/1‑150 (MQ=255)
              tGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTAcc                                                                                                                                         <  1:1509198/150‑1 (MQ=255)
                ccGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAACACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACTGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGttcactg                                                                                                                                       >  1:1107573/1‑145 (MQ=255)
                ccGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCCGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTTTGGCTGGAGTTACCTg                                                                                                                                       >  2:385595/1‑150 (MQ=255)
                ccGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTg                                                                                                                                       >  2:190500/1‑150 (MQ=255)
                ccGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTg                                                                                                                                       <  1:1625851/150‑1 (MQ=255)
                    tCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCTAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTTCGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTaa                                                                                                                                   <  1:1062922/150‑1 (MQ=255)
                    tCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAAATATGGCTGGAGTTACCTGGTaa                                                                                                                                   >  1:1106972/1‑150 (MQ=255)
                     cGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAAc                                                                                                                                  <  1:1006386/150‑1 (MQ=255)
                      gAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATAAATTATGGCTGGAGTTACCTGGTAAcc                                                                                                                                 >  2:559459/1‑150 (MQ=255)
                          tAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGccc                                                                                                                             <  1:1886570/150‑1 (MQ=255)
                            gCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCTAAACCAGCCAACTGTCTACCGACGACATTAATCAGGCCATCACCCCAGCCGATCGGGAAAAATCGCGGGCTGCCTGCCAAAAATTATGCCTGGAGTTACCTGCTAACCGCCcac                                                                                                                           >  1:1182772/1‑150 (MQ=255)
                            gCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCcac                                                                                                                           <  2:657746/150‑1 (MQ=255)
                            gCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCcac                                                                                                                           <  1:962657/150‑1 (MQ=255)
                                 aTGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACAt                                                                                                                      >  1:497120/1‑150 (MQ=255)
                                            aaCCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGTCATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAAc                                                                                                           <  1:1362928/150‑1 (MQ=255)
                                              ccATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGc                                                                                                         >  1:2054348/1‑150 (MQ=255)
                                              ccATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTAAGGCCATCACCCCAGCGGTACGGGAATAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGc                                                                                                         >  2:2130180/1‑150 (MQ=255)
                                              ccATAGCATTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACAGAATTAAGGCCATCACCACAGCGGTACGGGAAAAGTCGCTGTCTGCCAGGCAAAATTGATCCATGAAGTTAGATGGTAACCGAACACTACAGGACTCCGGAACGc                                                                                                         >  2:887859/1‑150 (MQ=255)
                                               cATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAAAGcc                                                                                                        <  1:1277410/150‑1 (MQ=255)
                                               cATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTATGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGcc                                                                                                        >  2:1700166/1‑150 (MQ=255)
                                                 tCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGTCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCgg                                                                                                      <  1:375586/150‑1 (MQ=255)
                                                  cGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGt                                                                                                     <  2:2076077/150‑1 (MQ=255)
                                                   cccTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACTGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTg                                                                                                    <  2:263823/149‑1 (MQ=255)
                                                    ccTTAAGCGGCAATACCAGCGAACTGTCTACCGACGACATTATTCAGGACATCAACCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGc                                                                                                   <  1:1583974/150‑1 (MQ=255)
                                                    ccTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGc                                                                                                   <  2:23177/150‑1 (MQ=255)
                                                      ttAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTg                                                                                                 >  1:2179110/1‑150 (MQ=255)
                                                      ttAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTg                                                                                                 <  1:1808855/150‑1 (MQ=255)
                                                       tAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGa                                                                                                >  2:2210989/1‑150 (MQ=255)
                                                        aaGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGac                                                                                               <  1:576839/150‑1 (MQ=255)
                                                         aGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCCCCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCGAAAAATTATGGCTGGAGTTCCCTGGTAACCGCCCACAACATGCCGCCGGACCGCCGGTGGTGcca                                                                                              >  2:127510/1‑150 (MQ=255)
                                                          gCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACACCATGCCGCCGGACCGCCGGTGCTGacac                                                                                             >  1:767542/1‑150 (MQ=255)
                                                          gCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGacac                                                                                             >  1:1283648/1‑150 (MQ=255)
                                                            ggCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCCGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTACCCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTg                                                                                           >  1:1754716/1‑150 (MQ=255)
                                                             gCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTgg                                                                                          <  2:405280/150‑1 (MQ=255)
                                                                    cttcGAACTGTCTACCGACTAAATTATTCAGGCCATACCCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCt                                                                                   <  2:2182687/147‑1 (MQ=255)
                                                                     aGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAAAATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTg                                                                                  <  1:166984/150‑1 (MQ=255)
                                                                         aaCTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCg                                                                              <  2:1249634/150‑1 (MQ=255)
                                                                           cTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGGGCTGACACTGGAAAATCTGACAGGc                                                                            >  1:2173476/1‑150 (MQ=255)
                                                                              tCTACCGACGACATTATTCAGGCCACCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGaa                                                                         <  2:1532399/150‑1 (MQ=255)
                                                                               cTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCACTCTGCCAGCCAAAAATTATGGCAGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAg                                                                        >  1:636704/1‑150 (MQ=255)
                                                                                tACCGACGACATTATTCCGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAgg                                                                       >  2:681371/1‑150 (MQ=255)
                                                                                 acagacgacATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGCCCGGCGAAGGt                                                                      >  1:362834/4‑150 (MQ=255)
                                                                                  ccGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGtt                                                                     >  1:2041288/1‑150 (MQ=255)
                                                                                   cgacgaCATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGttt                                                                    >  1:338481/1‑150 (MQ=255)
                                                                                     acgacATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGTAAAATCTGACCGGCGAAGGTTTct                                                                  <  1:274158/150‑2 (MQ=255)
                                                                                        acATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGa                                                               >  1:13664/1‑150 (MQ=255)
                                                                                        acATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGaa                                                              >  2:786518/1‑150 (MQ=255)
                                                                                               tCAGGCCATCACCCCAGCGGTACGGGAAAAATCTCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAAGCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAg                                                        <  1:1762027/150‑1 (MQ=255)
                                                                                                cAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGCGTTACCTGGTAACCGCCCCCAACATGCCGCCGGAACGCCGGTGCTGAGACTGGAAAATCTGACCGGCGAAGGTTTCAGGACTGTCAGc                                                       >  1:188745/1‑150 (MQ=255)
                                                                                                cAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTCCCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGCCCGGCGAAGCTTTCAGGAATGTCAGc                                                       >  1:513345/1‑150 (MQ=255)
                                                                                                 aGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGcc                                                      <  1:617706/150‑1 (MQ=255)
                                                                                                 aGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAAACGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGct                                                      <  2:1351832/150‑2 (MQ=255)
                                                                                                  ggCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCt                                                     <  1:170055/150‑1 (MQ=255)
                                                                                                   gCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACCACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTg                                                    >  2:927439/1‑150 (MQ=255)
                                                                                                    ccATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGa                                                   >  1:1594614/1‑150 (MQ=255)
                                                                                                     cATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGAc                                                  <  2:535471/150‑1 (MQ=255)
                                                                                                         aCCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTc                                              >  1:1776209/1‑150 (MQ=255)
                                                                                                          ccccAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAAATATGGCTGGAGTTACCTGGAAACCGCCCACAACATGCCGCCGGACCGCCGGTGCTCACACTGGATAATCTGACCGGCGAAGGATACAGGAATGTCCCCCTGACGCTcc                                             >  1:430002/1‑149 (MQ=255)
                                                                                                            ccAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAAt                                           >  2:89540/1‑150 (MQ=255)
                                                                                                             cAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATg                                          >  1:2060780/1‑150 (MQ=255)
                                                                                                              aGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGc                                         <  1:252635/150‑1 (MQ=255)
                                                                                                                 ggTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCAGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCgg                                      >  1:1546572/1‑150 (MQ=255)
                                                                                                                 ggTAAGGGAAAAAGCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCgg                                      <  2:1329342/150‑1 (MQ=255)
                                                                                                                   tACGGGAAAAATCGCTCTCTGCCAGCCAAAAAATATGGCTGGATTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATTCCGgag                                    <  2:1146864/150‑1 (MQ=255)
                                                                                                                       ggAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACTCTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAAt                                >  2:1178988/1‑150 (MQ=255)
                                                                                                                       ggAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAAt                                >  1:1993189/1‑150 (MQ=255)
                                                                                                                        gAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAAtt                               >  1:781160/1‑150 (MQ=255)
                                                                                                                         aaaaaTCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTc                              <  2:46597/150‑1 (MQ=255)
                                                                                                                           aaaTCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTg                            >  2:832683/1‑150 (MQ=255)
                                                                                                                           aaaTCGCTCTCTGCCACGCAAAATATATGGCTGGAGTAACCTGGAAACCGCCCACACCATGCCGCCGGAACGCCGGTGCTGACACTGGAAAAACTGCCCGGCGATGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTg                            <  2:1340406/150‑1 (MQ=255)
                                                                                                                            aaTCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTgg                           <  1:787592/150‑1 (MQ=255)
                                                                                                                             aTCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTggg                          >  2:2194262/1‑150 (MQ=255)
                                                                                                                             aTCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAAACGACCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCACTGCCGGAGAAATTCAggg                          >  1:698570/1‑150 (MQ=255)
                                                                                                                                 ctctctGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCctg                      >  1:956766/1‑150 (MQ=255)
                                                                                                                                     ctGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCctggctg                  <  2:2089516/150‑1 (MQ=255)
                                                                                                                                     ctGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGGTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCctggctg                  <  1:952393/150‑1 (MQ=255)
                                                                                                                                      tGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGACGCCGGAACGACGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCctggctgg                 >  2:192444/1‑150 (MQ=255)
                                                                                                                                       tccagccaTAATTTTTGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTggctggg                >  2:1905295/2‑150 (MQ=255)
                                                                                                                                        ccagccaTAATTTTTGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTggctgggc               >  1:2130340/1‑150 (MQ=255)
                                                                                                                                        ccagccaAAAATTCAGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTggctgggc               <  2:1475568/150‑1 (MQ=255)
                                                                                                                                         cagccaAAATTTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGCACGCCGGTGCGGACACTGGAAACTCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCGCAATGCCGGAGAAATTCTGGGCCTggctgggct              <  2:317917/150‑1 (MQ=255)
                                                                                                                                         cagccaAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTggctgggct              <  2:1214873/150‑1 (MQ=255)
                                                                                                                                         cagcaaATAATTATGCGTGGATTTACCCGGTAACCGCCCACCACCTGCCGCCGGAACGCCGGTGCTGACACTCGAAAATCTGACCGGCGAAGGTTTCAGGACTGTCAGCCTGACGCTCAATGCCGCAGAAATTCTGGGCCTggctgggct              <  2:218398/150‑1 (MQ=255)
                                                                                                                                          agcaaAACATTATGGCTGGAGTTACCTGGTAACAGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCGGCCGGACGCTCAATGCCGGAGACATTCTGGGCCTggctcggctg             <  2:1396434/150‑1 (MQ=255)
                                                                                                                                           gccaAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTGgctgggctgg            >  2:418738/1‑150 (MQ=255)
                                                                                                                                            ccaAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTGGCTGGGCtggt           >  1:634455/1‑150 (MQ=255)
                                                                                                                                              aaaaaTTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGTTGCTGACACTGGAAAATCTGACCGGCGATGGTTACAGGAATGTCCGCAGGACGCTCAATGCCGGAGCAATTATGGGCCTGGCTGGGCtgttgg         >  2:1109380/1‑150 (MQ=255)
                                                                                                                                                aaaTTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTGGCTGGGCTGGTgggg       <  2:623277/150‑1 (MQ=255)
                                                                                                                                                   ttATGGCTGGAGTTACCTGGTAACCGCCCACAACAAGCCGCCGGAACGCCGGTGCGGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTGGCTGGGCTGGTGGGGGcc    <  1:1334886/150‑1 (MQ=255)
                                                                                                                                                     aTGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTTGCTGGGCTGGTGGGGGCCgg  >  2:487679/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTGGCTGGGCTGGTGGGGGCCGG  >  NC_000913/1602093‑1602391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: